Candidatus Viridilinea mediisalina

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Chloroflexi; Chloroflexia; Chloroflexales; Chloroflexineae; Oscillochloridaceae; Candidatus Viridilinea

Average proteome isoelectric point is 6.36

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4287 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2A6RGH5|A0A2A6RGH5_9CHLR NHase_alpha domain-containing protein OS=Candidatus Viridilinea mediisalina OX=2024553 GN=CJ255_16240 PE=4 SV=1
MM1 pKa = 7.51LAACGGGTQPAAAPPAPPAEE21 pKa = 4.67AEE23 pKa = 4.59DD24 pKa = 3.86VTEE27 pKa = 5.36PDD29 pKa = 3.28ATDD32 pKa = 3.74DD33 pKa = 5.47AEE35 pKa = 4.39ATDD38 pKa = 4.42DD39 pKa = 5.76AEE41 pKa = 4.52ATDD44 pKa = 4.73DD45 pKa = 5.45ADD47 pKa = 3.76VTDD50 pKa = 4.88DD51 pKa = 4.24AEE53 pKa = 4.48EE54 pKa = 4.16PAAPADD60 pKa = 3.98PADD63 pKa = 4.01GPMLYY68 pKa = 10.15YY69 pKa = 10.84AFGGGPVGGVFQVYY83 pKa = 10.43AEE85 pKa = 4.18ALSLIINAAVPNVDD99 pKa = 3.66LTAEE103 pKa = 4.33GTGGSAEE110 pKa = 4.12NLRR113 pKa = 11.84SVNSGDD119 pKa = 3.42RR120 pKa = 11.84QFGIVYY126 pKa = 10.33AGDD129 pKa = 3.33VSLGAEE135 pKa = 4.04GLLPEE140 pKa = 5.71DD141 pKa = 3.9ANQYY145 pKa = 8.16TNVMPVAPLYY155 pKa = 10.95GGVIHH160 pKa = 6.67LVVSRR165 pKa = 11.84ASGIEE170 pKa = 3.99TVADD174 pKa = 3.82LPGKK178 pKa = 10.47RR179 pKa = 11.84IALGNAGSGAALSAEE194 pKa = 4.14RR195 pKa = 11.84YY196 pKa = 7.44FNHH199 pKa = 6.64MGLMDD204 pKa = 4.01QVNVEE209 pKa = 4.16YY210 pKa = 10.74LGYY213 pKa = 9.47SQAAAAMGDD222 pKa = 3.75GQLDD226 pKa = 4.11GFWILAGFPNASVTEE241 pKa = 4.08ASTYY245 pKa = 9.73TDD247 pKa = 2.43IRR249 pKa = 11.84LIDD252 pKa = 3.7VYY254 pKa = 11.49NPGVEE259 pKa = 4.12GGFFEE264 pKa = 6.01AYY266 pKa = 9.17PFYY269 pKa = 10.98SFRR272 pKa = 11.84ALDD275 pKa = 3.66GGAYY279 pKa = 9.23PGNPEE284 pKa = 4.74DD285 pKa = 3.96VPSFQDD291 pKa = 3.18TALWVAHH298 pKa = 6.23TDD300 pKa = 3.4VPEE303 pKa = 4.23DD304 pKa = 3.39VVYY307 pKa = 10.86NALRR311 pKa = 11.84AVFLDD316 pKa = 3.63GGLDD320 pKa = 3.23RR321 pKa = 11.84MVATHH326 pKa = 7.42ASAQEE331 pKa = 3.84MGLEE335 pKa = 3.99VGITGIANQLHH346 pKa = 6.65PGAVRR351 pKa = 11.84FWEE354 pKa = 4.33EE355 pKa = 3.65QGVTIPDD362 pKa = 3.54EE363 pKa = 4.14LRR365 pKa = 4.8

Molecular weight: 37.9 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2A6RJL5|A0A2A6RJL5_9CHLR Uncharacterized protein OS=Candidatus Viridilinea mediisalina OX=2024553 GN=CJ255_09795 PE=4 SV=1
MM1 pKa = 7.94RR2 pKa = 11.84SQHH5 pKa = 5.94ILASSRR11 pKa = 11.84PRR13 pKa = 11.84LLAPSPPRR21 pKa = 11.84ALASSPPRR29 pKa = 11.84LLASSPPRR37 pKa = 11.84LLASSPPRR45 pKa = 11.84LLASSPPRR53 pKa = 11.84LLASSPPRR61 pKa = 11.84LLASSPPRR69 pKa = 11.84LLASSPPRR77 pKa = 11.84LL78 pKa = 3.53

Molecular weight: 8.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4287

0

4287

1557175

25

6103

363.2

39.82

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.698 ± 0.053

0.886 ± 0.013

4.917 ± 0.037

5.814 ± 0.035

3.328 ± 0.024

7.552 ± 0.044

2.361 ± 0.019

4.96 ± 0.032

1.94 ± 0.026

12.017 ± 0.06

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.13 ± 0.016

2.794 ± 0.037

5.846 ± 0.038

4.537 ± 0.027

7.246 ± 0.04

5.223 ± 0.029

5.571 ± 0.045

7.051 ± 0.03

1.496 ± 0.017

2.632 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski