Roseomonas rhizosphaerae

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodospirillales; Acetobacteraceae; Roseomonas

Average proteome isoelectric point is 7.06

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4203 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2C6Z8G4|A0A2C6Z8G4_9PROT Uncharacterized protein OS=Roseomonas rhizosphaerae OX=1335062 GN=CR162_11595 PE=4 SV=1
MM1 pKa = 7.53TSHH4 pKa = 6.75SLASFAEE11 pKa = 4.97GNASGAADD19 pKa = 3.18QSASVEE25 pKa = 4.06ASASASGDD33 pKa = 3.15GGSFAFSSATVSIEE47 pKa = 3.84GGSDD51 pKa = 2.82RR52 pKa = 11.84GDD54 pKa = 3.99FYY56 pKa = 11.88QHH58 pKa = 6.82AEE60 pKa = 4.11AEE62 pKa = 4.29ASAGGAGEE70 pKa = 4.51GMPPDD75 pKa = 3.72VPGYY79 pKa = 8.34TPPLEE84 pKa = 4.29EE85 pKa = 3.9EE86 pKa = 4.83PGFGWHH92 pKa = 7.09LPGLDD97 pKa = 4.34LGFLL101 pKa = 3.93

Molecular weight: 9.95 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2C7A6D9|A0A2C7A6D9_9PROT Inosine-5'-monophosphate dehydrogenase OS=Roseomonas rhizosphaerae OX=1335062 GN=guaB PE=3 SV=1
MM1 pKa = 7.59AGHH4 pKa = 6.97RR5 pKa = 11.84AARR8 pKa = 11.84SWPGAAARR16 pKa = 11.84GPGPGSRR23 pKa = 11.84RR24 pKa = 11.84ARR26 pKa = 11.84HH27 pKa = 5.91HH28 pKa = 6.25ARR30 pKa = 11.84HH31 pKa = 5.85HH32 pKa = 5.71ARR34 pKa = 11.84RR35 pKa = 11.84HH36 pKa = 4.54ARR38 pKa = 11.84CGGSSGGPRR47 pKa = 11.84GGAGRR52 pKa = 11.84HH53 pKa = 5.18SRR55 pKa = 11.84HH56 pKa = 5.25AAQRR60 pKa = 11.84RR61 pKa = 11.84GPRR64 pKa = 11.84RR65 pKa = 11.84LRR67 pKa = 11.84HH68 pKa = 5.36PAA70 pKa = 3.07

Molecular weight: 7.48 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4203

0

4203

1364214

31

2506

324.6

34.77

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

15.058 ± 0.071

0.826 ± 0.01

4.732 ± 0.026

5.852 ± 0.036

3.194 ± 0.024

9.55 ± 0.044

2.054 ± 0.017

3.823 ± 0.027

1.94 ± 0.03

11.335 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.414 ± 0.017

1.968 ± 0.022

6.378 ± 0.036

3.027 ± 0.022

8.542 ± 0.045

4.397 ± 0.023

4.607 ± 0.021

7.096 ± 0.03

1.455 ± 0.015

1.751 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski