Flavobacterium faecale
Average proteome isoelectric point is 6.64
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3658 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2S1LDH4|A0A2S1LDH4_9FLAO DDE_3 domain-containing protein OS=Flavobacterium faecale OX=1355330 GN=FFWV33_09635 PE=4 SV=1
MM1 pKa = 7.36 KK2 pKa = 10.41 KK3 pKa = 10.26 SVLALLFLLSLNSYY17 pKa = 9.67 AQFSTTHH24 pKa = 6.45 YY25 pKa = 9.89 IPPVTCNNNLAEE37 pKa = 4.31 DD38 pKa = 3.83 QYY40 pKa = 11.69 FYY42 pKa = 10.93 ISTPSISPVNFKK54 pKa = 10.55 IIAIGGATVTGVVSNTLPYY73 pKa = 9.78 IYY75 pKa = 10.35 SIGTGSSTQLMTPKK89 pKa = 9.65 STIGKK94 pKa = 7.83 LTNKK98 pKa = 10.09 GYY100 pKa = 10.2 IIEE103 pKa = 4.99 AEE105 pKa = 4.09 NQVYY109 pKa = 10.36 VSMRR113 pKa = 11.84 LNSSRR118 pKa = 11.84 NTSSGYY124 pKa = 9.11 NHH126 pKa = 7.44 AGGIVSKK133 pKa = 10.88 GNSALGQTFRR143 pKa = 11.84 LGAMLNPVNTDD154 pKa = 2.75 GFLLNFGSILATDD167 pKa = 3.68 NNTTVTISNIPTGTLMTDD185 pKa = 3.54 GTTITGPITINLNKK199 pKa = 10.28 NEE201 pKa = 4.27 SYY203 pKa = 11.38 VLAFEE208 pKa = 4.84 NGTGSVSNSAKK219 pKa = 10.22 MIGALVQTDD228 pKa = 3.56 KK229 pKa = 11.24 PVVVNSGSFGGSNSTSLNGRR249 pKa = 11.84 DD250 pKa = 3.25 VGFDD254 pKa = 3.2 QIVSFEE260 pKa = 4.29 KK261 pKa = 10.07 TGKK264 pKa = 10.32 EE265 pKa = 3.91 YY266 pKa = 10.64 IFVKK270 pKa = 10.69 GAGTNDD276 pKa = 3.15 IEE278 pKa = 4.31 RR279 pKa = 11.84 VQLIAQVDD287 pKa = 3.65 NTIVYY292 pKa = 10.06 INGSTTPFATLNAGQYY308 pKa = 11.32 ADD310 pKa = 3.32 ITGSSFTNGNLYY322 pKa = 10.28 VQTSEE327 pKa = 4.72 PVFAYY332 pKa = 10.17 QSIGGAANPANQNLFFVPPLNCTTPKK358 pKa = 10.24 IVDD361 pKa = 4.25 NIPLVDD367 pKa = 5.52 LIGSNSFSDD376 pKa = 3.28 NFLNIVTEE384 pKa = 4.44 TGATVSINNSVLTSSPVPVSGSPGFEE410 pKa = 3.67 RR411 pKa = 11.84 YY412 pKa = 7.33 TVNSLSGNISVKK424 pKa = 7.36 STRR427 pKa = 11.84 QVYY430 pKa = 9.64 VSYY433 pKa = 10.54 FGKK436 pKa = 10.42 NGAATYY442 pKa = 10.05 GGYY445 pKa = 10.72 YY446 pKa = 10.15 SGFDD450 pKa = 3.55 TKK452 pKa = 11.18 PEE454 pKa = 4.34 IISNKK459 pKa = 8.11 ITTTNSSCIPNVEE472 pKa = 4.5 LNVNTISSYY481 pKa = 11.83 DD482 pKa = 3.45 SFQWFSNGVPISGQIANSFIPTTPGYY508 pKa = 9.26 YY509 pKa = 9.66 NVRR512 pKa = 11.84 GTVSNCPLSQPLFSDD527 pKa = 4.71 EE528 pKa = 4.7 IPVSEE533 pKa = 5.36 CPTDD537 pKa = 3.48 QDD539 pKa = 3.89 GDD541 pKa = 4.16 GVNDD545 pKa = 5.75 NIDD548 pKa = 4.02 LDD550 pKa = 4.0 NDD552 pKa = 3.27 NDD554 pKa = 5.05 GITNCTEE561 pKa = 3.97 SYY563 pKa = 11.1 GNQAINLTNSSSGTIAVGTYY583 pKa = 6.77 TTTFTGATTTSAAVSTTPFVGAADD607 pKa = 3.94 GTFVTQIPAGKK618 pKa = 10.15 DD619 pKa = 3.09 SFVKK623 pKa = 10.54 QKK625 pKa = 9.72 LTFSQPMSVALEE637 pKa = 4.14 YY638 pKa = 10.4 VTTANSTDD646 pKa = 3.77 LLNANAEE653 pKa = 4.29 YY654 pKa = 10.23 IINSDD659 pKa = 3.64 TNKK662 pKa = 10.07 TITVSNPSNQLLIDD676 pKa = 3.65 TNYY679 pKa = 11.16 DD680 pKa = 3.43 GIYY683 pKa = 10.24 EE684 pKa = 4.32 SGVTQYY690 pKa = 11.65 SSFEE694 pKa = 3.32 IRR696 pKa = 11.84 FRR698 pKa = 11.84 LNAVVPLPAGTGDD711 pKa = 3.77 FRR713 pKa = 11.84 FSSYY717 pKa = 9.07 LTNTISITHH726 pKa = 6.6 KK727 pKa = 10.82 NLTDD731 pKa = 3.31 SDD733 pKa = 4.52 ANISTFKK740 pKa = 11.01 LIATCVPIDD749 pKa = 3.6 SDD751 pKa = 4.21 GDD753 pKa = 3.77 GKK755 pKa = 10.94 ADD757 pKa = 4.61 QVDD760 pKa = 3.88 TDD762 pKa = 4.08 SDD764 pKa = 3.85 NDD766 pKa = 4.63 GILDD770 pKa = 4.19 NIEE773 pKa = 4.06 AQVNNAVSITNADD786 pKa = 3.8 TNKK789 pKa = 10.85 DD790 pKa = 3.61 GIDD793 pKa = 3.38 NAFGSGFTPVDD804 pKa = 3.34 TDD806 pKa = 3.4 GDD808 pKa = 4.53 GIPDD812 pKa = 3.98 YY813 pKa = 11.55 LDD815 pKa = 4.77 LDD817 pKa = 4.09 SDD819 pKa = 3.97 NDD821 pKa = 4.59 GINDD825 pKa = 3.59 IEE827 pKa = 4.21 EE828 pKa = 4.5 SGTNATNKK836 pKa = 10.71 DD837 pKa = 3.17 IDD839 pKa = 3.62 NDD841 pKa = 4.4 GIMNYY846 pKa = 9.94 RR847 pKa = 11.84 EE848 pKa = 4.34 LDD850 pKa = 3.58 SDD852 pKa = 4.23 NDD854 pKa = 3.51 LCLDD858 pKa = 3.76 VIEE861 pKa = 6.12 AGFLDD866 pKa = 4.41 PNNDD870 pKa = 4.01 GILGNSPVTVNTKK883 pKa = 10.22 GQVTSAVGYY892 pKa = 5.54 TTPNNNYY899 pKa = 7.95 KK900 pKa = 8.92 TAAPIIITNQPNAPATCEE918 pKa = 3.9 LQQAIIPVVANGDD931 pKa = 3.73 SYY933 pKa = 11.42 QWQVSSNGTTWTAITNGTTYY953 pKa = 11.44 SNATTNQLNVNSVTTAMNGYY973 pKa = 9.4 KK974 pKa = 10.25 YY975 pKa = 10.23 RR976 pKa = 11.84 VFINRR981 pKa = 11.84 IGNSCGLLSSEE992 pKa = 4.54 TTLTVYY998 pKa = 9.52 PIPTVNDD1005 pKa = 3.01 ITLIQCDD1012 pKa = 4.49 DD1013 pKa = 4.32 DD1014 pKa = 6.12 LDD1016 pKa = 5.46 GITAFNLTEE1025 pKa = 4.0 KK1026 pKa = 11.06 NNIISANSTAEE1037 pKa = 3.6 TFTYY1041 pKa = 10.93 YY1042 pKa = 9.17 KK1043 pKa = 9.4 TQTAATNADD1052 pKa = 3.61 SSQLIPNPLSYY1063 pKa = 11.27 TNTTPLLMQVWARR1076 pKa = 11.84 VVNANNCFSVAEE1088 pKa = 4.09 MALKK1092 pKa = 10.51 VVATQVPATFNRR1104 pKa = 11.84 SFVTCDD1110 pKa = 3.98 DD1111 pKa = 3.83 EE1112 pKa = 6.96 LDD1114 pKa = 3.67 INGLNNVNNNKK1125 pKa = 9.52 RR1126 pKa = 11.84 DD1127 pKa = 3.6 GVSAFNFSSAEE1138 pKa = 3.89 NDD1140 pKa = 2.95 IKK1142 pKa = 11.27 ALLPSTGNYY1151 pKa = 10.23 SITFYY1156 pKa = 10.97 RR1157 pKa = 11.84 NEE1159 pKa = 3.78 ADD1161 pKa = 5.27 ALSEE1165 pKa = 4.13 QNKK1168 pKa = 7.8 ITSTTNYY1175 pKa = 10.32 RR1176 pKa = 11.84 NIGYY1180 pKa = 8.62 PNQQQIWGRR1189 pKa = 11.84 VDD1191 pKa = 3.92 SDD1193 pKa = 4.09 TDD1195 pKa = 3.44 NSCYY1199 pKa = 10.79 GFGPYY1204 pKa = 8.13 ITLTVEE1210 pKa = 4.18 QLPTANPVTIARR1222 pKa = 11.84 QCDD1225 pKa = 3.62 DD1226 pKa = 3.69 NQDD1229 pKa = 3.71 GIFIFNTATLEE1240 pKa = 4.07 NTLVNGQINVTATYY1254 pKa = 10.07 FDD1256 pKa = 4.12 AMNNPLKK1263 pKa = 10.61 DD1264 pKa = 3.77 ANGLLISSRR1273 pKa = 11.84 FPATFSTKK1281 pKa = 10.05 SQTIKK1286 pKa = 10.97 AVLTNNTSSKK1296 pKa = 10.82 CFDD1299 pKa = 3.42 DD1300 pKa = 3.61 TLITFTVDD1308 pKa = 2.88 VLPQAFSVPTSLTTVCDD1325 pKa = 4.17 DD1326 pKa = 4.01 EE1327 pKa = 6.07 LDD1329 pKa = 3.98 PVNQDD1334 pKa = 2.19 GRR1336 pKa = 11.84 YY1337 pKa = 9.79 SFNTSTFEE1345 pKa = 3.9 STIIGSQTGMVVSYY1359 pKa = 10.66 TDD1361 pKa = 3.15 QNGNLLSSPLPNPFNTNTQNITATVTNPINPNCTATTVIPFIINPLPQISLNADD1415 pKa = 3.21 GTEE1418 pKa = 4.58 DD1419 pKa = 4.71 EE1420 pKa = 5.59 LICSNLPTFFVQLNAGILDD1439 pKa = 4.32 GSPEE1443 pKa = 3.86 TNYY1446 pKa = 10.32 NFKK1449 pKa = 10.05 WSRR1452 pKa = 11.84 DD1453 pKa = 3.72 GIVLPTEE1460 pKa = 4.2 TNPTLDD1466 pKa = 3.54 VNTKK1470 pKa = 10.33 GIYY1473 pKa = 9.04 TVTVSFKK1480 pKa = 8.6 TTGCEE1485 pKa = 3.5 RR1486 pKa = 11.84 TRR1488 pKa = 11.84 TIVVTPSDD1496 pKa = 3.21 IAKK1499 pKa = 10.06 ISTIDD1504 pKa = 3.46 IEE1506 pKa = 5.64 DD1507 pKa = 3.41 MTDD1510 pKa = 2.79 INTVIVNVTGTGNYY1524 pKa = 9.6 VYY1526 pKa = 10.9 SLDD1529 pKa = 3.95 DD1530 pKa = 4.2 ANTFFQEE1537 pKa = 3.85 SNIFVNVPSGIHH1549 pKa = 6.34 DD1550 pKa = 3.86 VYY1552 pKa = 11.09 IKK1554 pKa = 10.59 DD1555 pKa = 3.67 VNGCGTVSQRR1565 pKa = 11.84 ISVIGLPKK1573 pKa = 10.49 FFTPNNDD1580 pKa = 3.04 GYY1582 pKa = 11.23 NDD1584 pKa = 3.4 YY1585 pKa = 11.01 WNVQGIDD1592 pKa = 3.3 STFNANTVIYY1602 pKa = 10.46 IFDD1605 pKa = 3.85 RR1606 pKa = 11.84 YY1607 pKa = 10.61 GKK1609 pKa = 9.92 LLKK1612 pKa = 10.11 QLNPLDD1618 pKa = 3.99 QGWDD1622 pKa = 3.11 GTYY1625 pKa = 10.92 NNKK1628 pKa = 9.35 PLPSDD1633 pKa = 4.15 DD1634 pKa = 3.17 YY1635 pKa = 9.97 WYY1637 pKa = 9.53 TVKK1640 pKa = 11.1 LEE1642 pKa = 4.5 DD1643 pKa = 3.17 GRR1645 pKa = 11.84 AAKK1648 pKa = 10.34 GHH1650 pKa = 6.26 FSLKK1654 pKa = 10.28 RR1655 pKa = 3.36
Molecular weight: 178.12 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.82
IPC2_protein 4.062
IPC_protein 4.101
Toseland 3.872
ProMoST 4.253
Dawson 4.088
Bjellqvist 4.24
Wikipedia 4.024
Rodwell 3.923
Grimsley 3.783
Solomon 4.088
Lehninger 4.05
Nozaki 4.19
DTASelect 4.482
Thurlkill 3.923
EMBOSS 4.037
Sillero 4.215
Patrickios 1.43
IPC_peptide 4.088
IPC2_peptide 4.202
IPC2.peptide.svr19 4.085
Protein with the highest isoelectric point:
>tr|A0A2S1LCK1|A0A2S1LCK1_9FLAO Acyl_transf_3 domain-containing protein OS=Flavobacterium faecale OX=1355330 GN=FFWV33_07730 PE=4 SV=1
MM1 pKa = 7.54 SVRR4 pKa = 11.84 KK5 pKa = 9.68 LKK7 pKa = 10.5 PITPGQRR14 pKa = 11.84 FRR16 pKa = 11.84 VVNGYY21 pKa = 9.53 DD22 pKa = 4.32 AITTDD27 pKa = 3.14 KK28 pKa = 10.95 PEE30 pKa = 4.34 RR31 pKa = 11.84 SLIAPIKK38 pKa = 10.47 NSGGRR43 pKa = 11.84 NSQGKK48 pKa = 6.33 MTMRR52 pKa = 11.84 YY53 pKa = 7.39 TGGGHH58 pKa = 4.86 KK59 pKa = 9.44 QRR61 pKa = 11.84 YY62 pKa = 8.74 RR63 pKa = 11.84 IIDD66 pKa = 3.76 FKK68 pKa = 10.67 RR69 pKa = 11.84 AKK71 pKa = 9.81 EE72 pKa = 4.23 GIPATVKK79 pKa = 10.68 SIEE82 pKa = 4.0 YY83 pKa = 10.15 DD84 pKa = 3.43 PNRR87 pKa = 11.84 TAFIALLAYY96 pKa = 10.31 ADD98 pKa = 3.96 GEE100 pKa = 4.24 KK101 pKa = 10.14 TYY103 pKa = 10.54 IIAQNGLKK111 pKa = 10.25 VGQKK115 pKa = 9.86 LVSGPEE121 pKa = 3.92 SQPEE125 pKa = 3.92 IGNTLPLSRR134 pKa = 11.84 IPLGTVISCIEE145 pKa = 3.9 LRR147 pKa = 11.84 PGQGAVIARR156 pKa = 11.84 SAGTFAQLMARR167 pKa = 11.84 DD168 pKa = 4.26 GKK170 pKa = 10.61 FATIKK175 pKa = 9.8 MPSGEE180 pKa = 4.18 TRR182 pKa = 11.84 LILLTCSATIGAVSNSDD199 pKa = 3.15 HH200 pKa = 6.2 QLVVSGKK207 pKa = 9.95 AGRR210 pKa = 11.84 TRR212 pKa = 11.84 WLGRR216 pKa = 11.84 RR217 pKa = 11.84 PRR219 pKa = 11.84 TRR221 pKa = 11.84 PVAMNPVDD229 pKa = 3.63 HH230 pKa = 7.13 PMGGGEE236 pKa = 4.08 GRR238 pKa = 11.84 SSGGHH243 pKa = 4.7 PRR245 pKa = 11.84 SRR247 pKa = 11.84 NGIPAKK253 pKa = 10.29 GYY255 pKa = 7.29 RR256 pKa = 11.84 TRR258 pKa = 11.84 SKK260 pKa = 10.85 KK261 pKa = 10.4 NPSNKK266 pKa = 10.11 YY267 pKa = 8.41 IVEE270 pKa = 4.07 RR271 pKa = 11.84 RR272 pKa = 11.84 KK273 pKa = 10.24 KK274 pKa = 10.1
Molecular weight: 29.83 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.308
IPC2_protein 9.97
IPC_protein 10.921
Toseland 11.096
ProMoST 10.994
Dawson 11.155
Bjellqvist 10.921
Wikipedia 11.418
Rodwell 11.359
Grimsley 11.184
Solomon 11.374
Lehninger 11.316
Nozaki 11.082
DTASelect 10.921
Thurlkill 11.082
EMBOSS 11.52
Sillero 11.096
Patrickios 11.067
IPC_peptide 11.374
IPC2_peptide 9.984
IPC2.peptide.svr19 8.468
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3658
0
3658
1287814
25
6201
352.1
39.56
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.786 ± 0.048
0.776 ± 0.014
5.386 ± 0.027
6.079 ± 0.052
5.22 ± 0.033
6.38 ± 0.048
1.658 ± 0.02
7.897 ± 0.039
7.895 ± 0.052
9.09 ± 0.051
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.233 ± 0.024
6.252 ± 0.044
3.388 ± 0.028
3.527 ± 0.026
3.133 ± 0.023
6.617 ± 0.043
6.226 ± 0.076
6.342 ± 0.035
1.046 ± 0.016
4.069 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here