Bifidobacterium vespertilionis
Average proteome isoelectric point is 6.08
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2281 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A5J5DU38|A0A5J5DU38_9BIFI DUF58 domain-containing protein OS=Bifidobacterium vespertilionis OX=2562524 GN=EM848_06660 PE=4 SV=1
MM1 pKa = 7.77 SDD3 pKa = 4.27 FEE5 pKa = 4.47 PFYY8 pKa = 11.06 DD9 pKa = 4.4 VNRR12 pKa = 11.84 TYY14 pKa = 11.52 DD15 pKa = 3.53 DD16 pKa = 4.03 NYY18 pKa = 11.15 ADD20 pKa = 4.03 GPFGAFAQALKK31 pKa = 10.8 AGDD34 pKa = 3.81 AAGSPAAGSPAEE46 pKa = 4.33 SAAVPAGVAGSPASGSPATDD66 pKa = 3.31 VPAGAASTPHH76 pKa = 5.6 VPP78 pKa = 4.21
Molecular weight: 7.51 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.748
IPC2_protein 3.795
IPC_protein 3.694
Toseland 3.49
ProMoST 3.923
Dawson 3.719
Bjellqvist 3.897
Wikipedia 3.732
Rodwell 3.541
Grimsley 3.414
Solomon 3.681
Lehninger 3.643
Nozaki 3.872
DTASelect 4.113
Thurlkill 3.592
EMBOSS 3.732
Sillero 3.834
Patrickios 1.901
IPC_peptide 3.681
IPC2_peptide 3.795
IPC2.peptide.svr19 3.762
Protein with the highest isoelectric point:
>tr|A0A5J5E1Z2|A0A5J5E1Z2_9BIFI Ketol-acid reductoisomerase (NADP(+)) OS=Bifidobacterium vespertilionis OX=2562524 GN=ilvC PE=3 SV=1
MM1 pKa = 7.44 KK2 pKa = 9.6 RR3 pKa = 11.84 TFQPNNRR10 pKa = 11.84 RR11 pKa = 11.84 RR12 pKa = 11.84 HH13 pKa = 4.83 MKK15 pKa = 9.27 HH16 pKa = 5.56 GFRR19 pKa = 11.84 VRR21 pKa = 11.84 MRR23 pKa = 11.84 TRR25 pKa = 11.84 SGRR28 pKa = 11.84 AVINARR34 pKa = 11.84 RR35 pKa = 11.84 NKK37 pKa = 9.32 GRR39 pKa = 11.84 KK40 pKa = 6.43 TLSAA44 pKa = 4.15
Molecular weight: 5.34 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.286
IPC_protein 12.881
Toseland 13.042
ProMoST 13.539
Dawson 13.042
Bjellqvist 13.042
Wikipedia 13.525
Rodwell 12.676
Grimsley 13.086
Solomon 13.539
Lehninger 13.437
Nozaki 13.042
DTASelect 13.042
Thurlkill 13.042
EMBOSS 13.539
Sillero 13.042
Patrickios 12.398
IPC_peptide 13.539
IPC2_peptide 12.53
IPC2.peptide.svr19 9.215
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2281
0
2281
834372
29
3152
365.8
39.79
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.643 ± 0.066
0.919 ± 0.017
6.673 ± 0.054
5.425 ± 0.048
3.433 ± 0.035
8.381 ± 0.042
2.08 ± 0.026
5.159 ± 0.045
3.476 ± 0.047
8.897 ± 0.048
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.545 ± 0.028
3.134 ± 0.031
4.911 ± 0.035
3.124 ± 0.029
6.455 ± 0.062
5.818 ± 0.042
5.928 ± 0.041
7.781 ± 0.042
1.476 ± 0.02
2.744 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here