Pelagibacter ubique (strain HTCC1062)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Pelagibacterales; Pelagibacteraceae; Candidatus Pelagibacter; Candidatus Pelagibacter ubique

Average proteome isoelectric point is 7.4

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1354 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q4FNR8|Q4FNR8_PELUB Predicted O-linked N-acetylglucosamine transferase OS=Pelagibacter ubique (strain HTCC1062) OX=335992 GN=SAR11_0349 PE=4 SV=1
MM1 pKa = 7.47NNLKK5 pKa = 10.62KK6 pKa = 10.46IGLTALAGCLAVTSVHH22 pKa = 6.48ASEE25 pKa = 4.6LTASGSASIGFGGADD40 pKa = 3.66KK41 pKa = 9.95GTSANGFYY49 pKa = 10.55MNDD52 pKa = 3.03EE53 pKa = 4.39VTFSGSGEE61 pKa = 4.4LDD63 pKa = 3.26NGWNVTLSMQVDD75 pKa = 3.47NNEE78 pKa = 4.08NVGTSTFDD86 pKa = 3.37NRR88 pKa = 11.84SVTINMGDD96 pKa = 3.08AGTFAFGGHH105 pKa = 6.24GLDD108 pKa = 3.61SVVGGVDD115 pKa = 3.42DD116 pKa = 5.03VMPTAYY122 pKa = 10.5GEE124 pKa = 4.1TWDD127 pKa = 4.59IISNTVDD134 pKa = 2.95NGGVTSTASTLFGAIGSAGSNNMMRR159 pKa = 11.84YY160 pKa = 10.43DD161 pKa = 3.25NTTAVEE167 pKa = 4.05GLKK170 pKa = 10.16ISASYY175 pKa = 10.19VPSGTGEE182 pKa = 4.09VEE184 pKa = 3.96SSVDD188 pKa = 3.25YY189 pKa = 10.8GLEE192 pKa = 3.85YY193 pKa = 10.25TGYY196 pKa = 10.43EE197 pKa = 4.27GLTLGYY203 pKa = 11.52AMGEE207 pKa = 4.02NNAAGGTSNTDD218 pKa = 2.69NDD220 pKa = 3.82TMYY223 pKa = 11.19VKK225 pKa = 10.46YY226 pKa = 10.25AYY228 pKa = 10.36GPVTVGYY235 pKa = 9.7QKK237 pKa = 10.9SEE239 pKa = 3.64IDD241 pKa = 3.15ATTATDD247 pKa = 3.43TDD249 pKa = 3.09EE250 pKa = 3.82WTAYY254 pKa = 9.24GVTYY258 pKa = 10.48AVSDD262 pKa = 4.03SLSVGYY268 pKa = 10.72AEE270 pKa = 4.2STYY273 pKa = 11.22DD274 pKa = 3.53AGSSTTDD281 pKa = 3.15QEE283 pKa = 4.21NSNLSVSYY291 pKa = 8.81TQGGMTLAAGFAEE304 pKa = 4.36EE305 pKa = 4.44KK306 pKa = 10.68NRR308 pKa = 11.84GGLTTAVNDD317 pKa = 3.42VKK319 pKa = 11.14GYY321 pKa = 10.59DD322 pKa = 3.17ISLAFAFF329 pKa = 4.83

Molecular weight: 33.89 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q4FM56|SYE_PELUB Glutamate--tRNA ligase OS=Pelagibacter ubique (strain HTCC1062) OX=335992 GN=gltX PE=3 SV=1
MM1 pKa = 7.64KK2 pKa = 10.45KK3 pKa = 8.01ITKK6 pKa = 9.47NIKK9 pKa = 10.2VKK11 pKa = 10.5VASNKK16 pKa = 10.07KK17 pKa = 9.31KK18 pKa = 10.44VSGNILNAPKK28 pKa = 8.77THH30 pKa = 6.82KK31 pKa = 10.45LPRR34 pKa = 11.84VVKK37 pKa = 10.3ARR39 pKa = 11.84AGQTITGKK47 pKa = 10.21IGGIRR52 pKa = 11.84KK53 pKa = 7.48TPNKK57 pKa = 10.19SIAKK61 pKa = 8.93PGQTITGKK69 pKa = 9.6IRR71 pKa = 11.84RR72 pKa = 11.84RR73 pKa = 11.84TNN75 pKa = 2.63

Molecular weight: 8.17 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1354

0

1354

416540

29

7317

307.6

34.68

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.717 ± 0.094

0.957 ± 0.026

5.402 ± 0.116

6.029 ± 0.083

5.219 ± 0.07

6.315 ± 0.076

1.565 ± 0.031

9.533 ± 0.105

10.231 ± 0.169

9.394 ± 0.094

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.224 ± 0.037

6.526 ± 0.055

3.191 ± 0.041

2.631 ± 0.03

3.117 ± 0.057

6.92 ± 0.096

5.112 ± 0.181

5.662 ± 0.06

0.906 ± 0.025

3.349 ± 0.037
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski