Amino acid dipepetide frequency for Pelagibacter ubique (strain HTCC1062)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.305AlaAla: 4.305 ± 0.154
0.653AlaCys: 0.653 ± 0.049
2.984AlaAsp: 2.984 ± 0.347
3.119AlaGlu: 3.119 ± 0.115
2.494AlaPhe: 2.494 ± 0.088
4.439AlaGly: 4.439 ± 0.121
0.869AlaHis: 0.869 ± 0.049
5.13AlaIle: 5.13 ± 0.146
4.962AlaLys: 4.962 ± 0.152
5.589AlaLeu: 5.589 ± 0.125
1.565AlaMet: 1.565 ± 0.064
2.898AlaAsn: 2.898 ± 0.107
1.676AlaPro: 1.676 ± 0.086
1.56AlaGln: 1.56 ± 0.07
2.067AlaArg: 2.067 ± 0.063
3.966AlaSer: 3.966 ± 0.189
3.056AlaThr: 3.056 ± 0.26
3.517AlaVal: 3.517 ± 0.14
0.523AlaTrp: 0.523 ± 0.043
1.798AlaTyr: 1.798 ± 0.067
0.0AlaXaa: 0.0 ± 0.0
Cys
0.567CysAla: 0.567 ± 0.043
0.122CysCys: 0.122 ± 0.018
0.583CysAsp: 0.583 ± 0.045
0.567CysGlu: 0.567 ± 0.043
0.495CysPhe: 0.495 ± 0.037
0.871CysGly: 0.871 ± 0.05
0.216CysHis: 0.216 ± 0.02
0.761CysIle: 0.761 ± 0.043
0.735CysLys: 0.735 ± 0.041
0.888CysLeu: 0.888 ± 0.045
0.178CysMet: 0.178 ± 0.021
0.547CysAsn: 0.547 ± 0.034
0.379CysPro: 0.379 ± 0.036
0.211CysGln: 0.211 ± 0.023
0.288CysArg: 0.288 ± 0.029
0.694CysSer: 0.694 ± 0.048
0.456CysThr: 0.456 ± 0.036
0.591CysVal: 0.591 ± 0.044
0.091CysTrp: 0.091 ± 0.016
0.329CysTyr: 0.329 ± 0.03
0.0CysXaa: 0.0 ± 0.0
Asp
3.075AspAla: 3.075 ± 0.379
0.574AspCys: 0.574 ± 0.04
2.823AspAsp: 2.823 ± 0.269
3.702AspGlu: 3.702 ± 0.112
2.965AspPhe: 2.965 ± 0.098
3.455AspGly: 3.455 ± 0.247
0.953AspHis: 0.953 ± 0.047
4.734AspIle: 4.734 ± 0.119
5.332AspLys: 5.332 ± 0.145
5.728AspLeu: 5.728 ± 0.117
1.066AspMet: 1.066 ± 0.056
2.984AspAsn: 2.984 ± 0.1
1.865AspPro: 1.865 ± 0.067
1.861AspGln: 1.861 ± 0.078
1.678AspArg: 1.678 ± 0.073
2.634AspSer: 2.634 ± 0.186
2.629AspThr: 2.629 ± 0.279
3.327AspVal: 3.327 ± 0.156
0.571AspTrp: 0.571 ± 0.038
2.065AspTyr: 2.065 ± 0.078
0.0AspXaa: 0.0 ± 0.0
Glu
3.608GluAla: 3.608 ± 0.123
0.497GluCys: 0.497 ± 0.037
3.241GluAsp: 3.241 ± 0.118
3.952GluGlu: 3.952 ± 0.132
2.782GluPhe: 2.782 ± 0.102
3.133GluGly: 3.133 ± 0.097
0.927GluHis: 0.927 ± 0.048
6.468GluIle: 6.468 ± 0.145
7.154GluLys: 7.154 ± 0.201
5.519GluLeu: 5.519 ± 0.155
1.431GluMet: 1.431 ± 0.06
4.506GluAsn: 4.506 ± 0.115
1.414GluPro: 1.414 ± 0.075
1.657GluGln: 1.657 ± 0.075
2.005GluArg: 2.005 ± 0.084
2.744GluSer: 2.744 ± 0.097
3.128GluThr: 3.128 ± 0.097
3.635GluVal: 3.635 ± 0.112
0.562GluTrp: 0.562 ± 0.035
1.93GluTyr: 1.93 ± 0.072
0.0GluXaa: 0.0 ± 0.0
Phe
2.578PheAla: 2.578 ± 0.099
0.557PheCys: 0.557 ± 0.04
3.087PheAsp: 3.087 ± 0.095
2.948PheGlu: 2.948 ± 0.095
3.383PhePhe: 3.383 ± 0.165
3.5PheGly: 3.5 ± 0.121
0.785PheHis: 0.785 ± 0.047
4.662PheIle: 4.662 ± 0.176
4.931PheLys: 4.931 ± 0.157
5.382PheLeu: 5.382 ± 0.197
1.049PheMet: 1.049 ± 0.058
3.531PheAsn: 3.531 ± 0.105
1.498PhePro: 1.498 ± 0.08
1.227PheGln: 1.227 ± 0.063
1.364PheArg: 1.364 ± 0.063
3.896PheSer: 3.896 ± 0.108
2.526PheThr: 2.526 ± 0.153
2.703PheVal: 2.703 ± 0.078
0.535PheTrp: 0.535 ± 0.041
2.045PheTyr: 2.045 ± 0.09
0.0PheXaa: 0.0 ± 0.0
Gly
4.091GlyAla: 4.091 ± 0.124
0.756GlyCys: 0.756 ± 0.042
3.102GlyAsp: 3.102 ± 0.124
3.282GlyGlu: 3.282 ± 0.091
3.222GlyPhe: 3.222 ± 0.105
4.626GlyGly: 4.626 ± 0.151
1.186GlyHis: 1.186 ± 0.058
5.62GlyIle: 5.62 ± 0.148
5.743GlyLys: 5.743 ± 0.15
5.776GlyLeu: 5.776 ± 0.145
1.673GlyMet: 1.673 ± 0.079
2.869GlyAsn: 2.869 ± 0.09
1.789GlyPro: 1.789 ± 0.076
1.695GlyGln: 1.695 ± 0.076
1.995GlyArg: 1.995 ± 0.082
4.576GlySer: 4.576 ± 0.311
3.774GlyThr: 3.774 ± 0.374
4.189GlyVal: 4.189 ± 0.104
0.749GlyTrp: 0.749 ± 0.049
2.442GlyTyr: 2.442 ± 0.082
0.0GlyXaa: 0.0 ± 0.0
His
0.931HisAla: 0.931 ± 0.054
0.194HisCys: 0.194 ± 0.022
0.754HisAsp: 0.754 ± 0.044
0.879HisGlu: 0.879 ± 0.05
0.727HisPhe: 0.727 ± 0.049
1.136HisGly: 1.136 ± 0.053
0.355HisHis: 0.355 ± 0.036
1.378HisIle: 1.378 ± 0.06
1.328HisLys: 1.328 ± 0.07
1.476HisLeu: 1.476 ± 0.054
0.391HisMet: 0.391 ± 0.035
0.845HisAsn: 0.845 ± 0.047
0.792HisPro: 0.792 ± 0.049
0.519HisGln: 0.519 ± 0.032
0.567HisArg: 0.567 ± 0.042
1.016HisSer: 1.016 ± 0.053
0.737HisThr: 0.737 ± 0.04
0.843HisVal: 0.843 ± 0.05
0.146HisTrp: 0.146 ± 0.023
0.636HisTyr: 0.636 ± 0.038
0.0HisXaa: 0.0 ± 0.0
Ile
5.418IleAla: 5.418 ± 0.128
0.996IleCys: 0.996 ± 0.053
5.505IleAsp: 5.505 ± 0.126
5.798IleGlu: 5.798 ± 0.149
5.258IlePhe: 5.258 ± 0.179
5.959IleGly: 5.959 ± 0.16
1.272IleHis: 1.272 ± 0.05
9.219IleIle: 9.219 ± 0.221
10.182IleLys: 10.182 ± 0.264
8.775IleLeu: 8.775 ± 0.241
1.762IleMet: 1.762 ± 0.067
6.612IleAsn: 6.612 ± 0.166
3.003IlePro: 3.003 ± 0.101
2.134IleGln: 2.134 ± 0.082
2.689IleArg: 2.689 ± 0.09
7.255IleSer: 7.255 ± 0.153
4.864IleThr: 4.864 ± 0.173
4.869IleVal: 4.869 ± 0.139
0.725IleTrp: 0.725 ± 0.045
3.032IleTyr: 3.032 ± 0.099
0.0IleXaa: 0.0 ± 0.0
Lys
4.336LysAla: 4.336 ± 0.145
0.629LysCys: 0.629 ± 0.039
5.999LysAsp: 5.999 ± 0.138
6.724LysGlu: 6.724 ± 0.199
4.895LysPhe: 4.895 ± 0.155
4.425LysGly: 4.425 ± 0.137
1.318LysHis: 1.318 ± 0.065
11.706LysIle: 11.706 ± 0.293
13.629LysLys: 13.629 ± 0.359
8.604LysLeu: 8.604 ± 0.197
2.211LysMet: 2.211 ± 0.091
9.24LysAsn: 9.24 ± 0.269
2.715LysPro: 2.715 ± 0.097
2.348LysGln: 2.348 ± 0.102
3.032LysArg: 3.032 ± 0.1
6.266LysSer: 6.266 ± 0.171
4.881LysThr: 4.881 ± 0.134
5.217LysVal: 5.217 ± 0.139
0.778LysTrp: 0.778 ± 0.05
3.354LysTyr: 3.354 ± 0.098
0.0LysXaa: 0.0 ± 0.0
Leu
5.594LeuAla: 5.594 ± 0.159
0.744LeuCys: 0.744 ± 0.045
5.094LeuAsp: 5.094 ± 0.131
5.546LeuGlu: 5.546 ± 0.162
4.717LeuPhe: 4.717 ± 0.173
5.887LeuGly: 5.887 ± 0.122
1.253LeuHis: 1.253 ± 0.061
9.348LeuIle: 9.348 ± 0.209
9.937LeuLys: 9.937 ± 0.246
7.81LeuLeu: 7.81 ± 0.21
2.127LeuMet: 2.127 ± 0.08
6.614LeuAsn: 6.614 ± 0.157
3.27LeuPro: 3.27 ± 0.106
2.209LeuGln: 2.209 ± 0.075
3.083LeuArg: 3.083 ± 0.092
7.502LeuSer: 7.502 ± 0.19
4.974LeuThr: 4.974 ± 0.16
5.114LeuVal: 5.114 ± 0.119
0.617LeuTrp: 0.617 ± 0.045
2.502LeuTyr: 2.502 ± 0.091
0.0LeuXaa: 0.0 ± 0.0
Met
1.476MetAla: 1.476 ± 0.059
0.197MetCys: 0.197 ± 0.022
1.114MetAsp: 1.114 ± 0.061
1.064MetGlu: 1.064 ± 0.057
0.984MetPhe: 0.984 ± 0.057
1.705MetGly: 1.705 ± 0.083
0.379MetHis: 0.379 ± 0.035
1.949MetIle: 1.949 ± 0.081
2.06MetLys: 2.06 ± 0.085
1.889MetLeu: 1.889 ± 0.082
0.6MetMet: 0.6 ± 0.041
1.457MetAsn: 1.457 ± 0.059
0.955MetPro: 0.955 ± 0.051
0.646MetGln: 0.646 ± 0.037
0.785MetArg: 0.785 ± 0.047
1.762MetSer: 1.762 ± 0.069
1.198MetThr: 1.198 ± 0.053
1.292MetVal: 1.292 ± 0.072
0.209MetTrp: 0.209 ± 0.026
0.519MetTyr: 0.519 ± 0.036
0.0MetXaa: 0.0 ± 0.0
Asn
2.72AsnAla: 2.72 ± 0.085
0.66AsnCys: 0.66 ± 0.044
3.186AsnAsp: 3.186 ± 0.207
4.1AsnGlu: 4.1 ± 0.152
4.06AsnPhe: 4.06 ± 0.111
3.032AsnGly: 3.032 ± 0.128
0.915AsnHis: 0.915 ± 0.054
6.609AsnIle: 6.609 ± 0.17
7.221AsnLys: 7.221 ± 0.211
7.037AsnLeu: 7.037 ± 0.185
1.371AsnMet: 1.371 ± 0.061
4.612AsnAsn: 4.612 ± 0.13
2.324AsnPro: 2.324 ± 0.087
1.993AsnGln: 1.993 ± 0.073
1.82AsnArg: 1.82 ± 0.073
4.561AsnSer: 4.561 ± 0.159
2.768AsnThr: 2.768 ± 0.084
3.061AsnVal: 3.061 ± 0.078
0.591AsnTrp: 0.591 ± 0.033
2.617AsnTyr: 2.617 ± 0.092
0.0AsnXaa: 0.0 ± 0.0
Pro
1.7ProAla: 1.7 ± 0.067
0.288ProCys: 0.288 ± 0.029
1.772ProAsp: 1.772 ± 0.065
2.293ProGlu: 2.293 ± 0.084
1.618ProPhe: 1.618 ± 0.062
2.103ProGly: 2.103 ± 0.086
0.562ProHis: 0.562 ± 0.037
2.946ProIle: 2.946 ± 0.102
3.02ProLys: 3.02 ± 0.108
2.886ProLeu: 2.886 ± 0.096
0.732ProMet: 0.732 ± 0.047
1.856ProAsn: 1.856 ± 0.069
0.871ProPro: 0.871 ± 0.052
0.771ProGln: 0.771 ± 0.044
0.972ProArg: 0.972 ± 0.051
2.197ProSer: 2.197 ± 0.081
1.777ProThr: 1.777 ± 0.064
2.072ProVal: 2.072 ± 0.083
0.375ProTrp: 0.375 ± 0.032
1.097ProTyr: 1.097 ± 0.063
0.0ProXaa: 0.0 ± 0.0
Gln
1.568GlnAla: 1.568 ± 0.061
0.206GlnCys: 0.206 ± 0.026
1.38GlnAsp: 1.38 ± 0.062
1.527GlnGlu: 1.527 ± 0.07
1.184GlnPhe: 1.184 ± 0.053
1.392GlnGly: 1.392 ± 0.066
0.396GlnHis: 0.396 ± 0.031
2.691GlnIle: 2.691 ± 0.073
2.835GlnLys: 2.835 ± 0.105
2.36GlnLeu: 2.36 ± 0.084
0.639GlnMet: 0.639 ± 0.038
1.954GlnAsn: 1.954 ± 0.062
0.783GlnPro: 0.783 ± 0.045
0.682GlnGln: 0.682 ± 0.045
0.943GlnArg: 0.943 ± 0.056
1.933GlnSer: 1.933 ± 0.075
1.356GlnThr: 1.356 ± 0.058
1.448GlnVal: 1.448 ± 0.068
0.242GlnTrp: 0.242 ± 0.029
0.79GlnTyr: 0.79 ± 0.044
0.0GlnXaa: 0.0 ± 0.0
Arg
1.928ArgAla: 1.928 ± 0.091
0.327ArgCys: 0.327 ± 0.029
1.685ArgAsp: 1.685 ± 0.066
2.002ArgGlu: 2.002 ± 0.08
1.596ArgPhe: 1.596 ± 0.068
1.942ArgGly: 1.942 ± 0.074
0.557ArgHis: 0.557 ± 0.037
2.787ArgIle: 2.787 ± 0.104
2.888ArgLys: 2.888 ± 0.1
2.89ArgLeu: 2.89 ± 0.101
0.759ArgMet: 0.759 ± 0.036
1.757ArgAsn: 1.757 ± 0.074
1.016ArgPro: 1.016 ± 0.058
0.943ArgGln: 0.943 ± 0.049
1.174ArgArg: 1.174 ± 0.065
2.12ArgSer: 2.12 ± 0.07
1.534ArgThr: 1.534 ± 0.065
1.913ArgVal: 1.913 ± 0.079
0.298ArgTrp: 0.298 ± 0.03
1.052ArgTyr: 1.052 ± 0.055
0.0ArgXaa: 0.0 ± 0.0
Ser
3.774SerAla: 3.774 ± 0.19
0.658SerCys: 0.658 ± 0.038
3.534SerAsp: 3.534 ± 0.242
4.098SerGlu: 4.098 ± 0.121
3.899SerPhe: 3.899 ± 0.109
4.825SerGly: 4.825 ± 0.165
1.162SerHis: 1.162 ± 0.05
6.247SerIle: 6.247 ± 0.13
7.051SerLys: 7.051 ± 0.183
6.897SerLeu: 6.897 ± 0.169
1.539SerMet: 1.539 ± 0.078
4.348SerAsn: 4.348 ± 0.16
2.019SerPro: 2.019 ± 0.074
1.81SerGln: 1.81 ± 0.06
2.041SerArg: 2.041 ± 0.085
5.202SerSer: 5.202 ± 0.333
3.33SerThr: 3.33 ± 0.131
3.839SerVal: 3.839 ± 0.126
0.636SerTrp: 0.636 ± 0.038
2.29SerTyr: 2.29 ± 0.093
0.0SerXaa: 0.0 ± 0.0
Thr
3.318ThrAla: 3.318 ± 0.296
0.475ThrCys: 0.475 ± 0.035
2.766ThrAsp: 2.766 ± 0.311
2.742ThrGlu: 2.742 ± 0.078
2.523ThrPhe: 2.523 ± 0.072
3.98ThrGly: 3.98 ± 0.245
0.869ThrHis: 0.869 ± 0.048
4.713ThrIle: 4.713 ± 0.134
4.369ThrLys: 4.369 ± 0.108
4.91ThrLeu: 4.91 ± 0.216
0.963ThrMet: 0.963 ± 0.044
2.948ThrAsn: 2.948 ± 0.098
2.053ThrPro: 2.053 ± 0.077
1.366ThrGln: 1.366 ± 0.079
1.517ThrArg: 1.517 ± 0.069
3.495ThrSer: 3.495 ± 0.129
2.706ThrThr: 2.706 ± 0.135
3.15ThrVal: 3.15 ± 0.381
0.451ThrTrp: 0.451 ± 0.034
1.808ThrTyr: 1.808 ± 0.311
0.0ThrXaa: 0.0 ± 0.0
Val
3.973ValAla: 3.973 ± 0.159
0.586ValCys: 0.586 ± 0.042
3.116ValAsp: 3.116 ± 0.107
3.474ValGlu: 3.474 ± 0.111
2.734ValPhe: 2.734 ± 0.085
3.98ValGly: 3.98 ± 0.105
0.864ValHis: 0.864 ± 0.048
5.061ValIle: 5.061 ± 0.119
4.922ValLys: 4.922 ± 0.146
4.864ValLeu: 4.864 ± 0.108
1.364ValMet: 1.364 ± 0.064
3.128ValAsn: 3.128 ± 0.179
2.045ValPro: 2.045 ± 0.074
1.354ValGln: 1.354 ± 0.064
1.745ValArg: 1.745 ± 0.067
4.18ValSer: 4.18 ± 0.141
3.344ValThr: 3.344 ± 0.391
3.656ValVal: 3.656 ± 0.118
0.499ValTrp: 0.499 ± 0.04
1.731ValTyr: 1.731 ± 0.081
0.0ValXaa: 0.0 ± 0.0
Trp
0.516TrpAla: 0.516 ± 0.035
0.13TrpCys: 0.13 ± 0.017
0.492TrpAsp: 0.492 ± 0.039
0.425TrpGlu: 0.425 ± 0.034
0.483TrpPhe: 0.483 ± 0.04
0.562TrpGly: 0.562 ± 0.038
0.209TrpHis: 0.209 ± 0.023
0.821TrpIle: 0.821 ± 0.047
0.787TrpLys: 0.787 ± 0.052
0.862TrpLeu: 0.862 ± 0.055
0.226TrpMet: 0.226 ± 0.023
0.576TrpAsn: 0.576 ± 0.039
0.305TrpPro: 0.305 ± 0.029
0.31TrpGln: 0.31 ± 0.028
0.348TrpArg: 0.348 ± 0.028
0.665TrpSer: 0.665 ± 0.045
0.435TrpThr: 0.435 ± 0.029
0.526TrpVal: 0.526 ± 0.047
0.094TrpTrp: 0.094 ± 0.016
0.293TrpTyr: 0.293 ± 0.029
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.695TyrAla: 1.695 ± 0.074
0.319TyrCys: 0.319 ± 0.033
1.803TyrAsp: 1.803 ± 0.064
2.053TyrGlu: 2.053 ± 0.079
2.17TyrPhe: 2.17 ± 0.096
2.201TyrGly: 2.201 ± 0.09
0.603TyrHis: 0.603 ± 0.04
2.475TyrIle: 2.475 ± 0.092
3.222TyrLys: 3.222 ± 0.101
3.798TyrLeu: 3.798 ± 0.119
0.593TyrMet: 0.593 ± 0.045
1.976TyrAsn: 1.976 ± 0.089
1.133TyrPro: 1.133 ± 0.05
1.016TyrGln: 1.016 ± 0.048
1.056TyrArg: 1.056 ± 0.055
2.535TyrSer: 2.535 ± 0.081
1.649TyrThr: 1.649 ± 0.223
1.661TyrVal: 1.661 ± 0.086
0.37TyrTrp: 0.37 ± 0.036
1.164TyrTyr: 1.164 ± 0.053
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 1354 proteins (416540 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski