Weissella paramesenteroides ATCC 33313
Average proteome isoelectric point is 6.47
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1952 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|C5RBC3|C5RBC3_WEIPA Ribosome-binding factor A OS=Weissella paramesenteroides ATCC 33313 OX=585506 GN=rbfA PE=3 SV=1
MM1 pKa = 7.42 YY2 pKa = 9.07 LTFSDD7 pKa = 3.68 TALQRR12 pKa = 11.84 ISPLVTTDD20 pKa = 2.86 TRR22 pKa = 11.84 IALDD26 pKa = 3.92 LDD28 pKa = 4.49 DD29 pKa = 5.18 GVGPFSNSATCTFDD43 pKa = 3.27 VAFNLVLCQASDD55 pKa = 3.7 LSDD58 pKa = 4.24 DD59 pKa = 3.91 FNMTIDD65 pKa = 3.71 SDD67 pKa = 4.0 LGPVYY72 pKa = 10.5 IKK74 pKa = 10.56 DD75 pKa = 3.71 YY76 pKa = 8.73 TTEE79 pKa = 4.05 QLDD82 pKa = 3.59 SALNLDD88 pKa = 3.26 VDD90 pKa = 3.47 HH91 pKa = 6.62 YY92 pKa = 11.59 LRR94 pKa = 11.84 YY95 pKa = 9.79 KK96 pKa = 10.85 LSGPSGILDD105 pKa = 3.53 PNVTLRR111 pKa = 11.84 NLQPVGG117 pKa = 3.42
Molecular weight: 12.84 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.71
IPC2_protein 3.745
IPC_protein 3.757
Toseland 3.516
ProMoST 3.973
Dawson 3.783
Bjellqvist 3.948
Wikipedia 3.795
Rodwell 3.579
Grimsley 3.427
Solomon 3.77
Lehninger 3.732
Nozaki 3.91
DTASelect 4.253
Thurlkill 3.605
EMBOSS 3.795
Sillero 3.884
Patrickios 1.926
IPC_peptide 3.757
IPC2_peptide 3.846
IPC2.peptide.svr19 3.796
Protein with the highest isoelectric point:
>tr|C5R9Z2|C5R9Z2_WEIPA NLPA lipoprotein OS=Weissella paramesenteroides ATCC 33313 OX=585506 GN=HMPREF0877_0787 PE=3 SV=1
MM1 pKa = 7.36 KK2 pKa = 9.43 RR3 pKa = 11.84 TYY5 pKa = 9.69 QPKK8 pKa = 8.34 KK9 pKa = 7.47 RR10 pKa = 11.84 HH11 pKa = 5.71 RR12 pKa = 11.84 EE13 pKa = 3.7 RR14 pKa = 11.84 VHH16 pKa = 6.23 GFRR19 pKa = 11.84 KK20 pKa = 10.04 RR21 pKa = 11.84 MSTSNGRR28 pKa = 11.84 KK29 pKa = 8.01 VLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 QKK37 pKa = 10.39 GRR39 pKa = 11.84 KK40 pKa = 8.52 VLSAA44 pKa = 4.05
Molecular weight: 5.38 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.335
IPC2_protein 10.862
IPC_protein 12.281
Toseland 12.442
ProMoST 12.925
Dawson 12.442
Bjellqvist 12.427
Wikipedia 12.91
Rodwell 12.193
Grimsley 12.486
Solomon 12.925
Lehninger 12.837
Nozaki 12.442
DTASelect 12.427
Thurlkill 12.442
EMBOSS 12.939
Sillero 12.442
Patrickios 11.93
IPC_peptide 12.939
IPC2_peptide 11.915
IPC2.peptide.svr19 9.043
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1952
0
1952
574627
39
2324
294.4
32.8
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.311 ± 0.07
0.24 ± 0.012
5.853 ± 0.05
5.576 ± 0.057
4.262 ± 0.036
6.706 ± 0.048
2.137 ± 0.028
7.331 ± 0.054
5.998 ± 0.058
9.701 ± 0.062
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.827 ± 0.029
4.801 ± 0.044
3.444 ± 0.031
4.614 ± 0.049
3.954 ± 0.043
5.943 ± 0.06
6.284 ± 0.039
7.389 ± 0.045
1.155 ± 0.022
3.475 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here