Olsenella umbonata
Average proteome isoelectric point is 5.97
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1943 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1G6N2N2|A0A1G6N2N2_9ACTN 2-iminobutanoate/2-iminopropanoate deaminase OS=Olsenella umbonata OX=604330 GN=SAMN04487824_12821 PE=3 SV=1
MM1 pKa = 7.5 HH2 pKa = 7.47 IEE4 pKa = 3.79 IDD6 pKa = 3.51 FDD8 pKa = 3.71 GTIVSGTLNDD18 pKa = 3.73 TEE20 pKa = 4.17 TAKK23 pKa = 11.04 AFFEE27 pKa = 4.26 ALPISIHH34 pKa = 6.0 VGSSGMDD41 pKa = 3.05 FCGQMEE47 pKa = 4.11 QSFPYY52 pKa = 10.38 DD53 pKa = 3.33 AAQVGYY59 pKa = 9.43 GWSNGDD65 pKa = 2.95 INYY68 pKa = 9.87 NPGGGWFAVFFDD80 pKa = 4.94 GEE82 pKa = 4.43 DD83 pKa = 3.35 ASGSYY88 pKa = 10.69 GDD90 pKa = 3.7 QVNMGRR96 pKa = 11.84 LDD98 pKa = 4.02 DD99 pKa = 4.44 GAIEE103 pKa = 4.17 TLHH106 pKa = 6.1 GLKK109 pKa = 10.39 GSYY112 pKa = 10.54 DD113 pKa = 3.37 VTIRR117 pKa = 11.84 KK118 pKa = 8.75 AA119 pKa = 3.03
Molecular weight: 12.77 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.778
IPC2_protein 3.986
IPC_protein 3.948
Toseland 3.732
ProMoST 4.113
Dawson 3.948
Bjellqvist 4.101
Wikipedia 3.897
Rodwell 3.77
Grimsley 3.643
Solomon 3.935
Lehninger 3.884
Nozaki 4.062
DTASelect 4.304
Thurlkill 3.795
EMBOSS 3.897
Sillero 4.075
Patrickios 1.939
IPC_peptide 3.923
IPC2_peptide 4.05
IPC2.peptide.svr19 3.956
Protein with the highest isoelectric point:
>tr|A0A1H1NZW5|A0A1H1NZW5_9ACTN ATP synthase subunit beta OS=Olsenella umbonata OX=604330 GN=atpD PE=3 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.42 RR3 pKa = 11.84 TYY5 pKa = 10.34 QPNKK9 pKa = 8.62 RR10 pKa = 11.84 KK11 pKa = 9.56 RR12 pKa = 11.84 AKK14 pKa = 8.76 THH16 pKa = 5.23 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.11 GGRR28 pKa = 11.84 AVLSRR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.83 GRR39 pKa = 11.84 KK40 pKa = 8.88 RR41 pKa = 11.84 LTVV44 pKa = 3.11
Molecular weight: 5.18 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.433
IPC2_protein 11.125
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.144
Rodwell 12.398
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.135
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.06
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1943
0
1943
645073
42
2028
332.0
36.09
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.509 ± 0.078
1.532 ± 0.026
6.568 ± 0.044
6.238 ± 0.061
3.366 ± 0.033
8.514 ± 0.045
2.017 ± 0.024
4.468 ± 0.051
3.899 ± 0.045
9.083 ± 0.065
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.685 ± 0.028
2.68 ± 0.029
4.381 ± 0.039
3.003 ± 0.032
6.649 ± 0.055
5.822 ± 0.054
5.303 ± 0.037
8.474 ± 0.048
1.052 ± 0.021
2.757 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here