Alteromonadaceae phage B23

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 6.3

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 50 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2P9JZJ2|A0A2P9JZJ2_9CAUD Putative major head subunit protein OS=Alteromonadaceae phage B23 OX=2094572 PE=4 SV=1
MM1 pKa = 8.09PLTQDD6 pKa = 3.95RR7 pKa = 11.84ITPVRR12 pKa = 11.84DD13 pKa = 3.68GEE15 pKa = 4.41VMPYY19 pKa = 10.36AVAAGVEE26 pKa = 4.32IFLGAQVAVNATGYY40 pKa = 10.0LVPGSEE46 pKa = 4.33ATTLTYY52 pKa = 10.44VGRR55 pKa = 11.84ADD57 pKa = 3.28AHH59 pKa = 6.3VDD61 pKa = 3.22NSEE64 pKa = 4.06GADD67 pKa = 3.55GDD69 pKa = 4.05EE70 pKa = 4.35TCLVRR75 pKa = 11.84RR76 pKa = 11.84KK77 pKa = 9.9KK78 pKa = 10.83AFLFKK83 pKa = 11.01NSDD86 pKa = 3.83ADD88 pKa = 3.84PVDD91 pKa = 3.44QSLVGQPCYY100 pKa = 10.42ILDD103 pKa = 4.31DD104 pKa = 3.92EE105 pKa = 5.09TVAGTDD111 pKa = 3.49GTGTRR116 pKa = 11.84SEE118 pKa = 4.48SGTVLAVEE126 pKa = 4.52SAGVWVEE133 pKa = 3.71

Molecular weight: 13.94 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2P9JZH9|A0A2P9JZH9_9CAUD Uncharacterized protein OS=Alteromonadaceae phage B23 OX=2094572 PE=4 SV=1
MM1 pKa = 7.17SHH3 pKa = 6.6NLASQSAEE11 pKa = 3.33ARR13 pKa = 11.84ADD15 pKa = 3.11IQRR18 pKa = 11.84EE19 pKa = 4.03KK20 pKa = 10.94KK21 pKa = 9.49RR22 pKa = 11.84WLEE25 pKa = 3.32AWLMVRR31 pKa = 11.84NRR33 pKa = 11.84HH34 pKa = 5.15PQWIRR39 pKa = 11.84HH40 pKa = 4.73WLTTIEE46 pKa = 4.98DD47 pKa = 3.6KK48 pKa = 10.94DD49 pKa = 4.67DD50 pKa = 4.62RR51 pKa = 11.84DD52 pKa = 3.76DD53 pKa = 3.25WRR55 pKa = 11.84HH56 pKa = 5.5RR57 pKa = 11.84LNTLRR62 pKa = 11.84QKK64 pKa = 10.96GG65 pKa = 3.47

Molecular weight: 8.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

50

0

50

10929

56

697

218.6

24.12

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.77 ± 0.421

0.686 ± 0.114

6.625 ± 0.228

7.082 ± 0.329

2.699 ± 0.262

8.308 ± 0.578

1.903 ± 0.178

4.493 ± 0.202

4.374 ± 0.39

8.802 ± 0.4

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.086 ± 0.16

3.495 ± 0.237

4.438 ± 0.245

4.648 ± 0.229

7.659 ± 0.352

5.261 ± 0.204

5.746 ± 0.268

6.844 ± 0.342

1.638 ± 0.162

2.443 ± 0.187
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski