Amino acid dipepetide frequency for Alteromonadaceae phage B23

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
12.262AlaAla: 12.262 ± 1.233
0.641AlaCys: 0.641 ± 0.23
7.138AlaAsp: 7.138 ± 0.692
8.876AlaGlu: 8.876 ± 1.212
3.203AlaPhe: 3.203 ± 0.621
10.615AlaGly: 10.615 ± 1.133
1.281AlaHis: 1.281 ± 0.327
4.941AlaIle: 4.941 ± 0.682
4.392AlaLys: 4.392 ± 0.702
9.334AlaLeu: 9.334 ± 0.994
3.203AlaMet: 3.203 ± 0.47
3.02AlaAsn: 3.02 ± 0.483
4.484AlaPro: 4.484 ± 0.646
5.673AlaGln: 5.673 ± 0.846
5.948AlaArg: 5.948 ± 0.774
5.307AlaSer: 5.307 ± 0.5
6.589AlaThr: 6.589 ± 0.658
7.321AlaVal: 7.321 ± 0.833
1.922AlaTrp: 1.922 ± 0.418
1.556AlaTyr: 1.556 ± 0.284
0.0AlaXaa: 0.0 ± 0.0
Cys
0.366CysAla: 0.366 ± 0.166
0.0CysCys: 0.0 ± 0.0
0.641CysAsp: 0.641 ± 0.189
0.183CysGlu: 0.183 ± 0.153
0.092CysPhe: 0.092 ± 0.081
0.275CysGly: 0.275 ± 0.167
0.275CysHis: 0.275 ± 0.14
0.275CysIle: 0.275 ± 0.184
0.549CysLys: 0.549 ± 0.269
1.007CysLeu: 1.007 ± 0.256
0.092CysMet: 0.092 ± 0.091
0.275CysAsn: 0.275 ± 0.16
0.458CysPro: 0.458 ± 0.178
0.275CysGln: 0.275 ± 0.137
0.732CysArg: 0.732 ± 0.214
0.458CysSer: 0.458 ± 0.158
0.183CysThr: 0.183 ± 0.13
0.183CysVal: 0.183 ± 0.131
0.0CysTrp: 0.0 ± 0.0
0.549CysTyr: 0.549 ± 0.201
0.0CysXaa: 0.0 ± 0.0
Asp
5.673AspAla: 5.673 ± 0.792
0.458AspCys: 0.458 ± 0.192
4.575AspAsp: 4.575 ± 0.693
4.484AspGlu: 4.484 ± 0.548
2.196AspPhe: 2.196 ± 0.412
6.131AspGly: 6.131 ± 0.7
1.19AspHis: 1.19 ± 0.352
3.477AspIle: 3.477 ± 0.488
2.105AspLys: 2.105 ± 0.419
7.046AspLeu: 7.046 ± 0.8
1.007AspMet: 1.007 ± 0.305
2.105AspAsn: 2.105 ± 0.491
2.562AspPro: 2.562 ± 0.447
2.837AspGln: 2.837 ± 0.501
5.124AspArg: 5.124 ± 0.741
3.203AspSer: 3.203 ± 0.367
4.941AspThr: 4.941 ± 0.801
3.935AspVal: 3.935 ± 0.565
1.281AspTrp: 1.281 ± 0.364
1.922AspTyr: 1.922 ± 0.322
0.0AspXaa: 0.0 ± 0.0
Glu
6.955GluAla: 6.955 ± 0.717
0.824GluCys: 0.824 ± 0.209
3.843GluAsp: 3.843 ± 0.573
4.758GluGlu: 4.758 ± 0.76
1.647GluPhe: 1.647 ± 0.387
4.85GluGly: 4.85 ± 0.554
1.739GluHis: 1.739 ± 0.453
3.843GluIle: 3.843 ± 0.58
3.02GluLys: 3.02 ± 0.616
7.046GluLeu: 7.046 ± 1.009
2.013GluMet: 2.013 ± 0.475
2.196GluAsn: 2.196 ± 0.426
2.928GluPro: 2.928 ± 0.682
3.294GluGln: 3.294 ± 0.474
6.314GluArg: 6.314 ± 0.733
4.667GluSer: 4.667 ± 0.777
3.935GluThr: 3.935 ± 0.62
4.026GluVal: 4.026 ± 0.568
1.464GluTrp: 1.464 ± 0.294
1.464GluTyr: 1.464 ± 0.342
0.0GluXaa: 0.0 ± 0.0
Phe
1.922PheAla: 1.922 ± 0.489
0.366PheCys: 0.366 ± 0.172
2.013PheAsp: 2.013 ± 0.42
2.105PheGlu: 2.105 ± 0.402
0.549PhePhe: 0.549 ± 0.271
2.196PheGly: 2.196 ± 0.499
0.641PheHis: 0.641 ± 0.232
0.824PheIle: 0.824 ± 0.258
1.83PheLys: 1.83 ± 0.398
2.105PheLeu: 2.105 ± 0.382
0.275PheMet: 0.275 ± 0.145
0.915PheAsn: 0.915 ± 0.293
0.641PhePro: 0.641 ± 0.274
1.19PheGln: 1.19 ± 0.292
2.013PheArg: 2.013 ± 0.429
2.013PheSer: 2.013 ± 0.398
2.288PheThr: 2.288 ± 0.488
1.373PheVal: 1.373 ± 0.428
0.549PheTrp: 0.549 ± 0.244
1.19PheTyr: 1.19 ± 0.47
0.0PheXaa: 0.0 ± 0.0
Gly
7.87GlyAla: 7.87 ± 1.794
0.366GlyCys: 0.366 ± 0.151
5.033GlyAsp: 5.033 ± 0.606
5.765GlyGlu: 5.765 ± 0.835
2.837GlyPhe: 2.837 ± 0.704
7.138GlyGly: 7.138 ± 1.107
1.373GlyHis: 1.373 ± 0.339
4.209GlyIle: 4.209 ± 0.558
2.928GlyLys: 2.928 ± 0.515
6.68GlyLeu: 6.68 ± 0.905
2.013GlyMet: 2.013 ± 0.411
3.294GlyAsn: 3.294 ± 0.6
2.745GlyPro: 2.745 ± 0.376
4.209GlyGln: 4.209 ± 0.682
7.504GlyArg: 7.504 ± 1.252
5.765GlySer: 5.765 ± 0.798
4.392GlyThr: 4.392 ± 0.74
5.216GlyVal: 5.216 ± 0.592
1.83GlyTrp: 1.83 ± 0.475
1.922GlyTyr: 1.922 ± 0.354
0.0GlyXaa: 0.0 ± 0.0
His
1.647HisAla: 1.647 ± 0.367
0.183HisCys: 0.183 ± 0.105
1.556HisAsp: 1.556 ± 0.35
0.458HisGlu: 0.458 ± 0.173
0.641HisPhe: 0.641 ± 0.198
1.647HisGly: 1.647 ± 0.434
0.915HisHis: 0.915 ± 0.366
0.824HisIle: 0.824 ± 0.249
0.732HisLys: 0.732 ± 0.297
1.922HisLeu: 1.922 ± 0.396
0.549HisMet: 0.549 ± 0.222
0.824HisAsn: 0.824 ± 0.291
1.464HisPro: 1.464 ± 0.351
0.824HisGln: 0.824 ± 0.27
1.556HisArg: 1.556 ± 0.285
0.824HisSer: 0.824 ± 0.236
0.915HisThr: 0.915 ± 0.299
0.915HisVal: 0.915 ± 0.229
0.366HisTrp: 0.366 ± 0.168
0.275HisTyr: 0.275 ± 0.184
0.0HisXaa: 0.0 ± 0.0
Ile
5.399IleAla: 5.399 ± 0.892
0.549IleCys: 0.549 ± 0.189
2.928IleAsp: 2.928 ± 0.507
3.752IleGlu: 3.752 ± 0.6
0.824IlePhe: 0.824 ± 0.339
3.569IleGly: 3.569 ± 0.647
1.647IleHis: 1.647 ± 0.342
1.556IleIle: 1.556 ± 0.381
1.647IleLys: 1.647 ± 0.367
3.111IleLeu: 3.111 ± 0.509
0.915IleMet: 0.915 ± 0.308
1.739IleAsn: 1.739 ± 0.353
1.556IlePro: 1.556 ± 0.278
2.013IleGln: 2.013 ± 0.424
3.66IleArg: 3.66 ± 0.584
2.196IleSer: 2.196 ± 0.366
3.02IleThr: 3.02 ± 0.405
3.111IleVal: 3.111 ± 0.474
0.275IleTrp: 0.275 ± 0.153
1.464IleTyr: 1.464 ± 0.33
0.0IleXaa: 0.0 ± 0.0
Lys
5.124LysAla: 5.124 ± 0.755
0.366LysCys: 0.366 ± 0.167
2.379LysAsp: 2.379 ± 0.382
2.379LysGlu: 2.379 ± 0.532
1.464LysPhe: 1.464 ± 0.484
2.745LysGly: 2.745 ± 0.496
0.458LysHis: 0.458 ± 0.175
2.562LysIle: 2.562 ± 0.422
3.111LysLys: 3.111 ± 0.686
3.66LysLeu: 3.66 ± 0.541
0.732LysMet: 0.732 ± 0.266
2.288LysAsn: 2.288 ± 0.441
3.111LysPro: 3.111 ± 0.568
2.105LysGln: 2.105 ± 0.414
2.928LysArg: 2.928 ± 0.596
1.647LysSer: 1.647 ± 0.342
2.928LysThr: 2.928 ± 0.598
2.288LysVal: 2.288 ± 0.449
0.732LysTrp: 0.732 ± 0.292
0.641LysTyr: 0.641 ± 0.219
0.0LysXaa: 0.0 ± 0.0
Leu
10.432LeuAla: 10.432 ± 0.942
0.366LeuCys: 0.366 ± 0.178
5.857LeuAsp: 5.857 ± 0.759
6.772LeuGlu: 6.772 ± 0.902
1.556LeuPhe: 1.556 ± 0.334
7.961LeuGly: 7.961 ± 1.375
1.19LeuHis: 1.19 ± 0.318
2.471LeuIle: 2.471 ± 0.445
4.118LeuLys: 4.118 ± 0.625
5.307LeuLeu: 5.307 ± 0.811
1.556LeuMet: 1.556 ± 0.318
3.935LeuAsn: 3.935 ± 0.562
4.392LeuPro: 4.392 ± 0.61
4.85LeuGln: 4.85 ± 0.662
6.223LeuArg: 6.223 ± 0.726
4.758LeuSer: 4.758 ± 0.791
5.948LeuThr: 5.948 ± 0.633
6.863LeuVal: 6.863 ± 0.993
1.098LeuTrp: 1.098 ± 0.362
2.379LeuTyr: 2.379 ± 0.59
0.0LeuXaa: 0.0 ± 0.0
Met
2.745MetAla: 2.745 ± 0.45
0.0MetCys: 0.0 ± 0.0
1.464MetAsp: 1.464 ± 0.363
1.647MetGlu: 1.647 ± 0.35
0.549MetPhe: 0.549 ± 0.247
1.464MetGly: 1.464 ± 0.438
0.183MetHis: 0.183 ± 0.135
0.915MetIle: 0.915 ± 0.258
1.647MetLys: 1.647 ± 0.374
1.098MetLeu: 1.098 ± 0.367
0.275MetMet: 0.275 ± 0.194
0.458MetAsn: 0.458 ± 0.204
1.739MetPro: 1.739 ± 0.436
0.549MetGln: 0.549 ± 0.222
1.83MetArg: 1.83 ± 0.398
1.83MetSer: 1.83 ± 0.334
1.007MetThr: 1.007 ± 0.281
1.19MetVal: 1.19 ± 0.343
0.092MetTrp: 0.092 ± 0.075
0.183MetTyr: 0.183 ± 0.139
0.0MetXaa: 0.0 ± 0.0
Asn
3.935AsnAla: 3.935 ± 0.572
0.183AsnCys: 0.183 ± 0.13
2.288AsnAsp: 2.288 ± 0.407
1.83AsnGlu: 1.83 ± 0.47
0.915AsnPhe: 0.915 ± 0.323
3.111AsnGly: 3.111 ± 0.576
0.641AsnHis: 0.641 ± 0.267
1.556AsnIle: 1.556 ± 0.534
1.19AsnLys: 1.19 ± 0.37
2.837AsnLeu: 2.837 ± 0.611
0.641AsnMet: 0.641 ± 0.262
1.281AsnAsn: 1.281 ± 0.307
2.196AsnPro: 2.196 ± 0.455
2.105AsnGln: 2.105 ± 0.488
2.562AsnArg: 2.562 ± 0.442
1.922AsnSer: 1.922 ± 0.453
1.464AsnThr: 1.464 ± 0.391
3.02AsnVal: 3.02 ± 0.492
0.366AsnTrp: 0.366 ± 0.168
0.915AsnTyr: 0.915 ± 0.242
0.0AsnXaa: 0.0 ± 0.0
Pro
4.575ProAla: 4.575 ± 0.678
0.366ProCys: 0.366 ± 0.183
4.118ProAsp: 4.118 ± 0.613
4.118ProGlu: 4.118 ± 0.698
0.824ProPhe: 0.824 ± 0.403
4.026ProGly: 4.026 ± 0.609
0.732ProHis: 0.732 ± 0.209
1.556ProIle: 1.556 ± 0.303
1.739ProLys: 1.739 ± 0.449
4.209ProLeu: 4.209 ± 0.666
0.641ProMet: 0.641 ± 0.223
1.83ProAsn: 1.83 ± 0.376
1.098ProPro: 1.098 ± 0.273
1.647ProGln: 1.647 ± 0.422
3.02ProArg: 3.02 ± 0.554
1.83ProSer: 1.83 ± 0.413
1.922ProThr: 1.922 ± 0.511
4.026ProVal: 4.026 ± 0.574
0.732ProTrp: 0.732 ± 0.288
1.373ProTyr: 1.373 ± 0.258
0.0ProXaa: 0.0 ± 0.0
Gln
6.497GlnAla: 6.497 ± 1.09
0.641GlnCys: 0.641 ± 0.2
3.294GlnAsp: 3.294 ± 0.594
3.02GlnGlu: 3.02 ± 0.603
0.824GlnPhe: 0.824 ± 0.245
2.654GlnGly: 2.654 ± 0.419
0.915GlnHis: 0.915 ± 0.302
2.379GlnIle: 2.379 ± 0.489
1.556GlnLys: 1.556 ± 0.341
4.392GlnLeu: 4.392 ± 0.534
1.281GlnMet: 1.281 ± 0.348
1.098GlnAsn: 1.098 ± 0.318
2.196GlnPro: 2.196 ± 0.432
2.196GlnGln: 2.196 ± 0.429
3.66GlnArg: 3.66 ± 0.669
2.013GlnSer: 2.013 ± 0.351
2.196GlnThr: 2.196 ± 0.373
3.386GlnVal: 3.386 ± 0.618
0.824GlnTrp: 0.824 ± 0.218
1.464GlnTyr: 1.464 ± 0.323
0.0GlnXaa: 0.0 ± 0.0
Arg
7.321ArgAla: 7.321 ± 0.728
0.458ArgCys: 0.458 ± 0.2
4.941ArgAsp: 4.941 ± 0.853
5.49ArgGlu: 5.49 ± 0.867
3.477ArgPhe: 3.477 ± 0.635
4.667ArgGly: 4.667 ± 0.758
2.196ArgHis: 2.196 ± 0.459
3.843ArgIle: 3.843 ± 0.729
3.569ArgLys: 3.569 ± 0.626
7.778ArgLeu: 7.778 ± 1.047
1.464ArgMet: 1.464 ± 0.361
2.837ArgAsn: 2.837 ± 0.593
3.111ArgPro: 3.111 ± 0.486
3.111ArgGln: 3.111 ± 0.579
7.046ArgArg: 7.046 ± 1.091
2.928ArgSer: 2.928 ± 0.63
3.569ArgThr: 3.569 ± 0.66
5.033ArgVal: 5.033 ± 0.715
1.556ArgTrp: 1.556 ± 0.359
2.196ArgTyr: 2.196 ± 0.366
0.0ArgXaa: 0.0 ± 0.0
Ser
5.948SerAla: 5.948 ± 0.874
0.366SerCys: 0.366 ± 0.197
2.745SerAsp: 2.745 ± 0.472
4.118SerGlu: 4.118 ± 0.551
1.281SerPhe: 1.281 ± 0.415
4.758SerGly: 4.758 ± 1.008
1.007SerHis: 1.007 ± 0.356
2.105SerIle: 2.105 ± 0.443
2.562SerLys: 2.562 ± 0.557
4.758SerLeu: 4.758 ± 0.672
1.007SerMet: 1.007 ± 0.27
1.922SerAsn: 1.922 ± 0.315
2.562SerPro: 2.562 ± 0.523
2.105SerGln: 2.105 ± 0.407
3.843SerArg: 3.843 ± 0.716
2.654SerSer: 2.654 ± 0.542
2.288SerThr: 2.288 ± 0.409
4.85SerVal: 4.85 ± 0.779
0.366SerTrp: 0.366 ± 0.153
1.373SerTyr: 1.373 ± 0.274
0.0SerXaa: 0.0 ± 0.0
Thr
6.863ThrAla: 6.863 ± 0.744
0.366ThrCys: 0.366 ± 0.171
4.209ThrAsp: 4.209 ± 0.744
3.752ThrGlu: 3.752 ± 0.721
1.556ThrPhe: 1.556 ± 0.377
6.772ThrGly: 6.772 ± 0.913
1.098ThrHis: 1.098 ± 0.284
2.562ThrIle: 2.562 ± 0.507
2.288ThrLys: 2.288 ± 0.538
4.85ThrLeu: 4.85 ± 0.685
1.007ThrMet: 1.007 ± 0.25
1.647ThrAsn: 1.647 ± 0.323
3.02ThrPro: 3.02 ± 0.488
2.379ThrGln: 2.379 ± 0.467
3.294ThrArg: 3.294 ± 0.544
2.013ThrSer: 2.013 ± 0.375
3.294ThrThr: 3.294 ± 0.453
4.118ThrVal: 4.118 ± 0.541
1.007ThrTrp: 1.007 ± 0.284
1.373ThrTyr: 1.373 ± 0.279
0.0ThrXaa: 0.0 ± 0.0
Val
8.51ValAla: 8.51 ± 1.02
0.183ValCys: 0.183 ± 0.124
4.85ValAsp: 4.85 ± 0.595
5.124ValGlu: 5.124 ± 0.613
1.83ValPhe: 1.83 ± 0.444
4.392ValGly: 4.392 ± 0.848
1.007ValHis: 1.007 ± 0.358
3.203ValIle: 3.203 ± 0.477
3.203ValLys: 3.203 ± 0.755
4.85ValLeu: 4.85 ± 0.674
1.281ValMet: 1.281 ± 0.361
2.196ValAsn: 2.196 ± 0.45
2.745ValPro: 2.745 ± 0.574
2.562ValGln: 2.562 ± 0.438
6.314ValArg: 6.314 ± 0.851
4.484ValSer: 4.484 ± 0.684
4.209ValThr: 4.209 ± 0.807
4.667ValVal: 4.667 ± 0.593
1.281ValTrp: 1.281 ± 0.286
1.556ValTyr: 1.556 ± 0.382
0.0ValXaa: 0.0 ± 0.0
Trp
1.464TrpAla: 1.464 ± 0.551
0.092TrpCys: 0.092 ± 0.084
0.824TrpAsp: 0.824 ± 0.338
0.732TrpGlu: 0.732 ± 0.273
0.275TrpPhe: 0.275 ± 0.18
0.915TrpGly: 0.915 ± 0.251
0.366TrpHis: 0.366 ± 0.152
0.915TrpIle: 0.915 ± 0.254
0.549TrpLys: 0.549 ± 0.22
2.745TrpLeu: 2.745 ± 0.507
0.458TrpMet: 0.458 ± 0.192
0.458TrpAsn: 0.458 ± 0.201
0.458TrpPro: 0.458 ± 0.232
1.19TrpGln: 1.19 ± 0.344
0.915TrpArg: 0.915 ± 0.267
0.915TrpSer: 0.915 ± 0.305
0.915TrpThr: 0.915 ± 0.249
1.464TrpVal: 1.464 ± 0.391
0.549TrpTrp: 0.549 ± 0.302
0.183TrpTyr: 0.183 ± 0.13
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.196TyrAla: 2.196 ± 0.407
0.092TyrCys: 0.092 ± 0.083
1.556TyrAsp: 1.556 ± 0.349
1.464TyrGlu: 1.464 ± 0.305
0.458TyrPhe: 0.458 ± 0.199
3.203TyrGly: 3.203 ± 0.433
0.183TyrHis: 0.183 ± 0.107
0.915TyrIle: 0.915 ± 0.342
1.007TyrLys: 1.007 ± 0.289
3.294TyrLeu: 3.294 ± 0.493
0.366TyrMet: 0.366 ± 0.162
0.641TyrAsn: 0.641 ± 0.217
0.915TyrPro: 0.915 ± 0.325
1.373TyrGln: 1.373 ± 0.372
2.105TyrArg: 2.105 ± 0.422
1.19TyrSer: 1.19 ± 0.266
1.464TyrThr: 1.464 ± 0.363
1.464TyrVal: 1.464 ± 0.347
0.092TyrTrp: 0.092 ± 0.102
0.458TyrTyr: 0.458 ± 0.179
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 50 proteins (10929 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski