Megasphaera sp. An286

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Negativicutes; Veillonellales; Veillonellaceae; Megasphaera; unclassified Megasphaera

Average proteome isoelectric point is 6.2

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2144 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Y4CH52|A0A1Y4CH52_9FIRM HIT family protein OS=Megasphaera sp. An286 OX=1965622 GN=B5F80_06020 PE=4 SV=1
MM1 pKa = 8.06DD2 pKa = 5.37KK3 pKa = 11.07YY4 pKa = 11.04EE5 pKa = 4.27CTICGYY11 pKa = 10.17IYY13 pKa = 10.77DD14 pKa = 4.21EE15 pKa = 5.22AEE17 pKa = 3.96GDD19 pKa = 3.72AEE21 pKa = 4.39NNVAAGTKK29 pKa = 9.78FADD32 pKa = 4.79LPEE35 pKa = 5.1DD36 pKa = 3.8WACPTCGAGKK46 pKa = 10.09DD47 pKa = 3.66AFVKK51 pKa = 10.03MDD53 pKa = 3.2

Molecular weight: 5.74 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Y4CXH8|A0A1Y4CXH8_9FIRM ATPase OS=Megasphaera sp. An286 OX=1965622 GN=B5F80_02655 PE=4 SV=1
MM1 pKa = 7.52TKK3 pKa = 10.43RR4 pKa = 11.84ITRR7 pKa = 11.84TGLLLAATLILQGLRR22 pKa = 11.84LFIPIPPQVSMFVIGSLVNACLVLAVWSVGRR53 pKa = 11.84KK54 pKa = 8.71SGLVVACVTPVFAWLEE70 pKa = 3.92GMLPFLPFIFPVAAGNCAFVFAVDD94 pKa = 3.37GLRR97 pKa = 11.84RR98 pKa = 11.84FRR100 pKa = 11.84AAGLAGAALCKK111 pKa = 10.19AAVVYY116 pKa = 10.64GSFYY120 pKa = 11.22VLFSYY125 pKa = 11.14VEE127 pKa = 4.19FPAAVRR133 pKa = 11.84HH134 pKa = 5.58MILLVMSWPQIVTAAAGGVLAYY156 pKa = 10.15VIAKK160 pKa = 9.53RR161 pKa = 11.84LPRR164 pKa = 11.84LEE166 pKa = 4.11GPRR169 pKa = 3.54

Molecular weight: 18.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2144

0

2144

691686

35

5663

322.6

35.66

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.55 ± 0.071

1.459 ± 0.028

5.785 ± 0.053

6.35 ± 0.055

3.858 ± 0.045

7.896 ± 0.072

2.093 ± 0.025

6.569 ± 0.043

5.428 ± 0.05

9.043 ± 0.067

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.089 ± 0.029

3.437 ± 0.063

3.883 ± 0.042

3.524 ± 0.036

5.123 ± 0.058

5.387 ± 0.038

5.295 ± 0.051

7.565 ± 0.046

0.996 ± 0.022

3.67 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski