Amino acid dipepetide frequency for Megasphaera sp. An286

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
12.193AlaAla: 12.193 ± 0.214
1.499AlaCys: 1.499 ± 0.054
5.705AlaAsp: 5.705 ± 0.114
6.066AlaGlu: 6.066 ± 0.119
3.694AlaPhe: 3.694 ± 0.08
7.923AlaGly: 7.923 ± 0.167
1.697AlaHis: 1.697 ± 0.047
5.527AlaIle: 5.527 ± 0.097
4.888AlaLys: 4.888 ± 0.088
8.927AlaLeu: 8.927 ± 0.127
3.159AlaMet: 3.159 ± 0.081
2.714AlaAsn: 2.714 ± 0.104
2.918AlaPro: 2.918 ± 0.079
3.165AlaGln: 3.165 ± 0.067
3.87AlaArg: 3.87 ± 0.085
4.661AlaSer: 4.661 ± 0.098
3.558AlaThr: 3.558 ± 0.084
9.065AlaVal: 9.065 ± 0.147
0.84AlaTrp: 0.84 ± 0.038
3.429AlaTyr: 3.429 ± 0.072
0.0AlaXaa: 0.0 ± 0.0
Cys
1.201CysAla: 1.201 ± 0.042
0.308CysCys: 0.308 ± 0.025
0.752CysAsp: 0.752 ± 0.036
0.646CysGlu: 0.646 ± 0.029
0.489CysPhe: 0.489 ± 0.029
1.6CysGly: 1.6 ± 0.063
0.361CysHis: 0.361 ± 0.022
1.024CysIle: 1.024 ± 0.037
0.516CysLys: 0.516 ± 0.032
1.301CysLeu: 1.301 ± 0.043
0.393CysMet: 0.393 ± 0.024
0.383CysAsn: 0.383 ± 0.024
0.792CysPro: 0.792 ± 0.04
0.505CysGln: 0.505 ± 0.027
1.187CysArg: 1.187 ± 0.054
0.867CysSer: 0.867 ± 0.042
0.691CysThr: 0.691 ± 0.032
0.998CysVal: 0.998 ± 0.042
0.124CysTrp: 0.124 ± 0.013
0.448CysTyr: 0.448 ± 0.025
0.0CysXaa: 0.0 ± 0.0
Asp
5.291AspAla: 5.291 ± 0.104
0.768AspCys: 0.768 ± 0.037
3.393AspAsp: 3.393 ± 0.087
4.55AspGlu: 4.55 ± 0.093
2.345AspPhe: 2.345 ± 0.057
5.007AspGly: 5.007 ± 0.133
1.0AspHis: 1.0 ± 0.041
4.637AspIle: 4.637 ± 0.08
3.188AspLys: 3.188 ± 0.079
4.605AspLeu: 4.605 ± 0.097
2.114AspMet: 2.114 ± 0.055
1.921AspAsn: 1.921 ± 0.064
1.875AspPro: 1.875 ± 0.05
1.17AspGln: 1.17 ± 0.048
2.358AspArg: 2.358 ± 0.055
2.732AspSer: 2.732 ± 0.076
3.056AspThr: 3.056 ± 0.071
5.067AspVal: 5.067 ± 0.103
0.64AspTrp: 0.64 ± 0.035
2.137AspTyr: 2.137 ± 0.055
0.0AspXaa: 0.0 ± 0.0
Glu
6.12GluAla: 6.12 ± 0.099
0.636GluCys: 0.636 ± 0.034
3.601GluAsp: 3.601 ± 0.079
5.193GluGlu: 5.193 ± 0.123
2.115GluPhe: 2.115 ± 0.061
4.676GluGly: 4.676 ± 0.084
1.323GluHis: 1.323 ± 0.043
3.97GluIle: 3.97 ± 0.08
4.382GluLys: 4.382 ± 0.081
5.943GluLeu: 5.943 ± 0.106
1.933GluMet: 1.933 ± 0.054
2.258GluAsn: 2.258 ± 0.074
2.052GluPro: 2.052 ± 0.069
2.913GluGln: 2.913 ± 0.069
3.82GluArg: 3.82 ± 0.1
2.741GluSer: 2.741 ± 0.063
3.46GluThr: 3.46 ± 0.071
3.546GluVal: 3.546 ± 0.075
0.577GluTrp: 0.577 ± 0.033
2.239GluTyr: 2.239 ± 0.059
0.0GluXaa: 0.0 ± 0.0
Phe
3.636PheAla: 3.636 ± 0.09
0.651PheCys: 0.651 ± 0.036
2.3PheAsp: 2.3 ± 0.048
1.772PheGlu: 1.772 ± 0.054
1.781PhePhe: 1.781 ± 0.063
3.194PheGly: 3.194 ± 0.068
0.948PheHis: 0.948 ± 0.038
2.689PheIle: 2.689 ± 0.073
1.518PheLys: 1.518 ± 0.052
3.7PheLeu: 3.7 ± 0.102
1.135PheMet: 1.135 ± 0.047
1.285PheAsn: 1.285 ± 0.046
1.514PhePro: 1.514 ± 0.048
1.155PheGln: 1.155 ± 0.038
1.719PheArg: 1.719 ± 0.049
2.73PheSer: 2.73 ± 0.071
2.248PheThr: 2.248 ± 0.057
2.789PheVal: 2.789 ± 0.074
0.421PheTrp: 0.421 ± 0.026
1.392PheTyr: 1.392 ± 0.044
0.0PheXaa: 0.0 ± 0.0
Gly
6.848GlyAla: 6.848 ± 0.136
1.327GlyCys: 1.327 ± 0.046
4.301GlyAsp: 4.301 ± 0.111
4.405GlyGlu: 4.405 ± 0.089
3.051GlyPhe: 3.051 ± 0.07
6.338GlyGly: 6.338 ± 0.171
1.637GlyHis: 1.637 ± 0.054
5.62GlyIle: 5.62 ± 0.089
4.986GlyLys: 4.986 ± 0.096
7.038GlyLeu: 7.038 ± 0.135
2.542GlyMet: 2.542 ± 0.061
2.77GlyAsn: 2.77 ± 0.136
2.033GlyPro: 2.033 ± 0.058
2.717GlyGln: 2.717 ± 0.066
4.2GlyArg: 4.2 ± 0.081
4.515GlySer: 4.515 ± 0.159
4.707GlyThr: 4.707 ± 0.115
6.108GlyVal: 6.108 ± 0.104
0.784GlyTrp: 0.784 ± 0.038
3.036GlyTyr: 3.036 ± 0.08
0.0GlyXaa: 0.0 ± 0.0
His
1.476HisAla: 1.476 ± 0.041
0.393HisCys: 0.393 ± 0.026
1.319HisAsp: 1.319 ± 0.042
1.125HisGlu: 1.125 ± 0.041
0.863HisPhe: 0.863 ± 0.038
1.758HisGly: 1.758 ± 0.057
0.575HisHis: 0.575 ± 0.034
1.745HisIle: 1.745 ± 0.048
0.951HisLys: 0.951 ± 0.034
1.758HisLeu: 1.758 ± 0.064
0.688HisMet: 0.688 ± 0.031
0.732HisAsn: 0.732 ± 0.033
1.068HisPro: 1.068 ± 0.041
0.606HisGln: 0.606 ± 0.029
1.053HisArg: 1.053 ± 0.045
1.07HisSer: 1.07 ± 0.038
1.178HisThr: 1.178 ± 0.038
1.574HisVal: 1.574 ± 0.046
0.256HisTrp: 0.256 ± 0.019
0.742HisTyr: 0.742 ± 0.033
0.0HisXaa: 0.0 ± 0.0
Ile
6.522IleAla: 6.522 ± 0.115
1.135IleCys: 1.135 ± 0.045
4.015IleAsp: 4.015 ± 0.084
3.771IleGlu: 3.771 ± 0.076
2.425IlePhe: 2.425 ± 0.073
5.455IleGly: 5.455 ± 0.107
1.452IleHis: 1.452 ± 0.045
4.155IleIle: 4.155 ± 0.1
2.717IleLys: 2.717 ± 0.061
5.974IleLeu: 5.974 ± 0.119
1.853IleMet: 1.853 ± 0.054
2.148IleAsn: 2.148 ± 0.073
3.016IlePro: 3.016 ± 0.078
2.111IleGln: 2.111 ± 0.06
3.4IleArg: 3.4 ± 0.08
4.159IleSer: 4.159 ± 0.079
3.649IleThr: 3.649 ± 0.101
5.105IleVal: 5.105 ± 0.086
0.561IleTrp: 0.561 ± 0.035
2.07IleTyr: 2.07 ± 0.06
0.0IleXaa: 0.0 ± 0.0
Lys
5.442LysAla: 5.442 ± 0.103
0.473LysCys: 0.473 ± 0.026
3.434LysAsp: 3.434 ± 0.075
4.723LysGlu: 4.723 ± 0.091
1.479LysPhe: 1.479 ± 0.05
4.013LysGly: 4.013 ± 0.084
0.956LysHis: 0.956 ± 0.039
3.334LysIle: 3.334 ± 0.064
4.133LysLys: 4.133 ± 0.102
4.174LysLeu: 4.174 ± 0.084
1.817LysMet: 1.817 ± 0.055
2.163LysAsn: 2.163 ± 0.064
1.903LysPro: 1.903 ± 0.056
2.018LysGln: 2.018 ± 0.061
2.835LysArg: 2.835 ± 0.073
2.419LysSer: 2.419 ± 0.066
2.991LysThr: 2.991 ± 0.071
3.478LysVal: 3.478 ± 0.078
0.5LysTrp: 0.5 ± 0.027
1.989LysTyr: 1.989 ± 0.064
0.0LysXaa: 0.0 ± 0.0
Leu
8.972LeuAla: 8.972 ± 0.148
1.538LeuCys: 1.538 ± 0.052
4.884LeuAsp: 4.884 ± 0.084
5.228LeuGlu: 5.228 ± 0.107
3.672LeuPhe: 3.672 ± 0.1
6.61LeuGly: 6.61 ± 0.119
2.046LeuHis: 2.046 ± 0.061
5.024LeuIle: 5.024 ± 0.11
4.85LeuLys: 4.85 ± 0.086
8.342LeuLeu: 8.342 ± 0.164
2.459LeuMet: 2.459 ± 0.065
3.013LeuAsn: 3.013 ± 0.071
4.203LeuPro: 4.203 ± 0.093
3.58LeuGln: 3.58 ± 0.078
5.077LeuArg: 5.077 ± 0.107
5.767LeuSer: 5.767 ± 0.109
5.361LeuThr: 5.361 ± 0.103
5.802LeuVal: 5.802 ± 0.099
0.885LeuTrp: 0.885 ± 0.037
3.114LeuTyr: 3.114 ± 0.072
0.0LeuXaa: 0.0 ± 0.0
Met
3.228MetAla: 3.228 ± 0.082
0.267MetCys: 0.267 ± 0.02
1.879MetAsp: 1.879 ± 0.057
2.232MetGlu: 2.232 ± 0.058
0.867MetPhe: 0.867 ± 0.034
2.179MetGly: 2.179 ± 0.068
0.577MetHis: 0.577 ± 0.029
1.853MetIle: 1.853 ± 0.054
2.388MetLys: 2.388 ± 0.055
2.636MetLeu: 2.636 ± 0.073
0.878MetMet: 0.878 ± 0.039
1.486MetAsn: 1.486 ± 0.043
1.411MetPro: 1.411 ± 0.048
1.0MetGln: 1.0 ± 0.04
1.365MetArg: 1.365 ± 0.048
1.657MetSer: 1.657 ± 0.056
2.004MetThr: 2.004 ± 0.049
1.822MetVal: 1.822 ± 0.064
0.179MetTrp: 0.179 ± 0.016
0.977MetTyr: 0.977 ± 0.037
0.0MetXaa: 0.0 ± 0.0
Asn
2.98AsnAla: 2.98 ± 0.104
0.492AsnCys: 0.492 ± 0.028
1.806AsnAsp: 1.806 ± 0.063
1.89AsnGlu: 1.89 ± 0.066
1.214AsnPhe: 1.214 ± 0.048
2.929AsnGly: 2.929 ± 0.118
0.729AsnHis: 0.729 ± 0.032
2.738AsnIle: 2.738 ± 0.089
1.79AsnLys: 1.79 ± 0.059
2.968AsnLeu: 2.968 ± 0.072
1.097AsnMet: 1.097 ± 0.042
1.251AsnAsn: 1.251 ± 0.071
1.836AsnPro: 1.836 ± 0.05
0.959AsnGln: 0.959 ± 0.036
1.697AsnArg: 1.697 ± 0.06
1.725AsnSer: 1.725 ± 0.073
1.903AsnThr: 1.903 ± 0.101
2.747AsnVal: 2.747 ± 0.11
0.37AsnTrp: 0.37 ± 0.024
1.253AsnTyr: 1.253 ± 0.045
0.0AsnXaa: 0.0 ± 0.0
Pro
3.627ProAla: 3.627 ± 0.085
0.499ProCys: 0.499 ± 0.025
2.602ProAsp: 2.602 ± 0.062
3.078ProGlu: 3.078 ± 0.073
1.778ProPhe: 1.778 ± 0.051
2.634ProGly: 2.634 ± 0.071
0.794ProHis: 0.794 ± 0.031
2.328ProIle: 2.328 ± 0.062
1.879ProLys: 1.879 ± 0.061
3.529ProLeu: 3.529 ± 0.084
1.162ProMet: 1.162 ± 0.043
1.266ProAsn: 1.266 ± 0.043
1.236ProPro: 1.236 ± 0.047
1.512ProGln: 1.512 ± 0.043
1.372ProArg: 1.372 ± 0.051
2.056ProSer: 2.056 ± 0.059
1.83ProThr: 1.83 ± 0.052
3.51ProVal: 3.51 ± 0.073
0.419ProTrp: 0.419 ± 0.024
1.72ProTyr: 1.72 ± 0.06
0.0ProXaa: 0.0 ± 0.0
Gln
3.769GlnAla: 3.769 ± 0.084
0.467GlnCys: 0.467 ± 0.023
1.814GlnAsp: 1.814 ± 0.058
2.383GlnGlu: 2.383 ± 0.061
1.394GlnPhe: 1.394 ± 0.039
2.594GlnGly: 2.594 ± 0.067
0.708GlnHis: 0.708 ± 0.031
2.28GlnIle: 2.28 ± 0.054
2.151GlnLys: 2.151 ± 0.058
2.903GlnLeu: 2.903 ± 0.073
1.041GlnMet: 1.041 ± 0.037
1.232GlnAsn: 1.232 ± 0.044
1.282GlnPro: 1.282 ± 0.045
1.478GlnGln: 1.478 ± 0.051
1.916GlnArg: 1.916 ± 0.059
1.72GlnSer: 1.72 ± 0.052
1.825GlnThr: 1.825 ± 0.058
2.225GlnVal: 2.225 ± 0.054
0.48GlnTrp: 0.48 ± 0.03
1.577GlnTyr: 1.577 ± 0.05
0.0GlnXaa: 0.0 ± 0.0
Arg
3.607ArgAla: 3.607 ± 0.079
0.808ArgCys: 0.808 ± 0.04
2.708ArgAsp: 2.708 ± 0.066
3.367ArgGlu: 3.367 ± 0.084
1.849ArgPhe: 1.849 ± 0.057
3.16ArgGly: 3.16 ± 0.075
1.248ArgHis: 1.248 ± 0.049
3.72ArgIle: 3.72 ± 0.085
2.837ArgLys: 2.837 ± 0.069
4.933ArgLeu: 4.933 ± 0.111
1.632ArgMet: 1.632 ± 0.054
1.861ArgAsn: 1.861 ± 0.05
2.053ArgPro: 2.053 ± 0.063
2.631ArgGln: 2.631 ± 0.071
4.229ArgArg: 4.229 ± 0.12
2.665ArgSer: 2.665 ± 0.057
2.445ArgThr: 2.445 ± 0.062
3.103ArgVal: 3.103 ± 0.078
0.526ArgTrp: 0.526 ± 0.028
1.846ArgTyr: 1.846 ± 0.06
0.0ArgXaa: 0.0 ± 0.0
Ser
4.72SerAla: 4.72 ± 0.105
0.732SerCys: 0.732 ± 0.037
2.955SerAsp: 2.955 ± 0.074
2.851SerGlu: 2.851 ± 0.068
2.482SerPhe: 2.482 ± 0.066
4.613SerGly: 4.613 ± 0.119
1.29SerHis: 1.29 ± 0.048
3.463SerIle: 3.463 ± 0.078
2.432SerLys: 2.432 ± 0.073
5.449SerLeu: 5.449 ± 0.117
1.629SerMet: 1.629 ± 0.054
1.683SerAsn: 1.683 ± 0.069
2.229SerPro: 2.229 ± 0.063
1.95SerGln: 1.95 ± 0.054
2.851SerArg: 2.851 ± 0.063
2.965SerSer: 2.965 ± 0.08
2.442SerThr: 2.442 ± 0.074
4.333SerVal: 4.333 ± 0.079
0.668SerTrp: 0.668 ± 0.034
2.135SerTyr: 2.135 ± 0.06
0.0SerXaa: 0.0 ± 0.0
Thr
5.573ThrAla: 5.573 ± 0.133
0.648ThrCys: 0.648 ± 0.027
2.938ThrAsp: 2.938 ± 0.068
3.144ThrGlu: 3.144 ± 0.072
2.062ThrPhe: 2.062 ± 0.059
4.663ThrGly: 4.663 ± 0.109
0.976ThrHis: 0.976 ± 0.036
3.742ThrIle: 3.742 ± 0.079
2.584ThrLys: 2.584 ± 0.063
4.891ThrLeu: 4.891 ± 0.1
1.642ThrMet: 1.642 ± 0.046
1.853ThrAsn: 1.853 ± 0.089
2.422ThrPro: 2.422 ± 0.061
1.397ThrGln: 1.397 ± 0.047
1.959ThrArg: 1.959 ± 0.051
2.555ThrSer: 2.555 ± 0.073
2.873ThrThr: 2.873 ± 0.086
4.515ThrVal: 4.515 ± 0.112
0.492ThrTrp: 0.492 ± 0.027
2.024ThrTyr: 2.024 ± 0.057
0.0ThrXaa: 0.0 ± 0.0
Val
6.299ValAla: 6.299 ± 0.114
1.258ValCys: 1.258 ± 0.047
4.269ValAsp: 4.269 ± 0.092
4.222ValGlu: 4.222 ± 0.079
3.066ValPhe: 3.066 ± 0.083
5.614ValGly: 5.614 ± 0.132
1.518ValHis: 1.518 ± 0.046
4.758ValIle: 4.758 ± 0.094
3.755ValLys: 3.755 ± 0.077
7.245ValLeu: 7.245 ± 0.113
2.344ValMet: 2.344 ± 0.07
2.588ValAsn: 2.588 ± 0.117
3.262ValPro: 3.262 ± 0.074
2.682ValGln: 2.682 ± 0.062
3.872ValArg: 3.872 ± 0.076
4.511ValSer: 4.511 ± 0.098
4.32ValThr: 4.32 ± 0.12
6.606ValVal: 6.606 ± 0.12
0.734ValTrp: 0.734 ± 0.037
2.728ValTyr: 2.728 ± 0.065
0.0ValXaa: 0.0 ± 0.0
Trp
0.802TrpAla: 0.802 ± 0.037
0.124TrpCys: 0.124 ± 0.015
0.609TrpAsp: 0.609 ± 0.029
0.551TrpGlu: 0.551 ± 0.028
0.484TrpPhe: 0.484 ± 0.028
0.704TrpGly: 0.704 ± 0.036
0.244TrpHis: 0.244 ± 0.02
0.603TrpIle: 0.603 ± 0.028
0.648TrpLys: 0.648 ± 0.03
0.985TrpLeu: 0.985 ± 0.043
0.308TrpMet: 0.308 ± 0.019
0.471TrpAsn: 0.471 ± 0.026
0.312TrpPro: 0.312 ± 0.021
0.546TrpGln: 0.546 ± 0.032
0.544TrpArg: 0.544 ± 0.031
0.502TrpSer: 0.502 ± 0.028
0.467TrpThr: 0.467 ± 0.028
0.526TrpVal: 0.526 ± 0.028
0.117TrpTrp: 0.117 ± 0.016
0.416TrpTyr: 0.416 ± 0.026
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.189TyrAla: 3.189 ± 0.071
0.565TyrCys: 0.565 ± 0.033
2.571TyrAsp: 2.571 ± 0.067
2.302TyrGlu: 2.302 ± 0.063
1.466TyrPhe: 1.466 ± 0.048
3.299TyrGly: 3.299 ± 0.074
0.852TyrHis: 0.852 ± 0.037
2.484TyrIle: 2.484 ± 0.066
1.683TyrLys: 1.683 ± 0.047
3.127TyrLeu: 3.127 ± 0.069
1.058TyrMet: 1.058 ± 0.037
1.294TyrAsn: 1.294 ± 0.056
1.418TyrPro: 1.418 ± 0.045
1.145TyrGln: 1.145 ± 0.042
1.904TyrArg: 1.904 ± 0.059
1.856TyrSer: 1.856 ± 0.057
1.943TyrThr: 1.943 ± 0.058
2.73TyrVal: 2.73 ± 0.068
0.39TyrTrp: 0.39 ± 0.029
1.427TyrTyr: 1.427 ± 0.046
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2144 proteins (691686 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski