Dechloromonas sp.
Average proteome isoelectric point is 6.82
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2873 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A6N1XG58|A0A6N1XG58_9RHOO Hydrolase or metal-binding protein OS=Dechloromonas sp. OX=1917218 GN=HT580_10515 PE=4 SV=1
MM1 pKa = 7.02 NTLLDD6 pKa = 3.82 ASISGTSPFAVTLTGLPAGTVVTGMMMTMVGGAPVWSAQGTGDD49 pKa = 3.96 NNALQALLNGITVTAPANANSNTGPFGFSATLTTYY84 pKa = 11.22 DD85 pKa = 4.33 DD86 pKa = 3.69 GGGRR90 pKa = 11.84 HH91 pKa = 6.64 DD92 pKa = 4.69 SNLTVSLPVTPVSDD106 pKa = 4.73 PIVLVATDD114 pKa = 4.07 SNVSEE119 pKa = 5.37 DD120 pKa = 3.53 SLAPISIALSNVADD134 pKa = 4.09 GANTNVVGGKK144 pKa = 9.96 VYY146 pKa = 10.64 VRR148 pKa = 11.84 LDD150 pKa = 3.55 EE151 pKa = 4.92 SGMEE155 pKa = 3.94 AAGGVLRR162 pKa = 11.84 FNGNPVAATSVSGVSGIADD181 pKa = 3.17 GTYY184 pKa = 9.77 FVLNGVSNGATLNLTYY200 pKa = 10.6 QPAANASGTVGYY212 pKa = 7.91 TAFVQNQEE220 pKa = 3.6 TGAANVATSSTSGSFDD236 pKa = 3.19 INKK239 pKa = 9.38 VNDD242 pKa = 3.97 GVTLSAGNVTGAEE255 pKa = 4.06 DD256 pKa = 3.73 QAVQLAISAALIDD269 pKa = 3.73 SGEE272 pKa = 4.41 RR273 pKa = 11.84 IQSVTLSHH281 pKa = 6.61 VPDD284 pKa = 4.72 GFQVYY289 pKa = 9.41 YY290 pKa = 11.26 GNGAPGTAATNMGGGVWSIPASAYY314 pKa = 9.91 AVPAYY319 pKa = 9.56 ISLVPPPNWSGTVSGVTADD338 pKa = 3.17 VWSGEE343 pKa = 4.18 TGLDD347 pKa = 3.38 QTVTSANFDD356 pKa = 3.52 VTFNGVADD364 pKa = 5.79 GILISPTLSFGNEE377 pKa = 3.98 GQVVPLNLNSAMPDD391 pKa = 3.02 MDD393 pKa = 3.66 GSEE396 pKa = 4.08 TAIITVKK403 pKa = 10.85 GLGSFAAFYY412 pKa = 10.52 AGSSLLSATYY422 pKa = 10.6 DD423 pKa = 3.49 SGLDD427 pKa = 3.39 TYY429 pKa = 9.61 TLSGLTPAQVSSLGVIQKK447 pKa = 10.41 DD448 pKa = 3.71 GSFDD452 pKa = 4.08 LVITAQTADD461 pKa = 3.66 SPGGNLSAVVNASMHH476 pKa = 5.98 VDD478 pKa = 3.77 INPVAATTGDD488 pKa = 3.63 DD489 pKa = 3.19 RR490 pKa = 11.84 LLYY493 pKa = 10.64 DD494 pKa = 4.06 GSALNGLVGSTPSNSAA510 pKa = 3.0
Molecular weight: 50.86 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.744
IPC2_protein 3.567
IPC_protein 3.605
Toseland 3.363
ProMoST 3.795
Dawson 3.617
Bjellqvist 3.783
Wikipedia 3.592
Rodwell 3.427
Grimsley 3.274
Solomon 3.605
Lehninger 3.567
Nozaki 3.732
DTASelect 4.037
Thurlkill 3.439
EMBOSS 3.605
Sillero 3.732
Patrickios 0.629
IPC_peptide 3.605
IPC2_peptide 3.706
IPC2.peptide.svr19 3.68
Protein with the highest isoelectric point:
>tr|A0A6N1XEQ9|A0A6N1XEQ9_9RHOO YceI family protein OS=Dechloromonas sp. OX=1917218 GN=HT580_07725 PE=4 SV=1
MM1 pKa = 6.82 STWIEE6 pKa = 3.85 SSIPFVAIFVMLIVGTDD23 pKa = 3.2 LRR25 pKa = 11.84 LADD28 pKa = 4.05 FQRR31 pKa = 11.84 VRR33 pKa = 11.84 RR34 pKa = 11.84 YY35 pKa = 9.74 LVLVPGIVCGQWLLLIGVAGLVGRR59 pKa = 11.84 LLDD62 pKa = 4.78 LPPGMTGGAILLAAAPVAAMSGFYY86 pKa = 9.06 TQLAGGHH93 pKa = 6.63 LALAVTVAAVSNLLAPIVTPVAASLGFWLFLDD125 pKa = 3.62 SDD127 pKa = 4.18 KK128 pKa = 11.8 AMEE131 pKa = 4.41 LPMLKK136 pKa = 10.01 VALQAIVGLLLPLLAGMLLRR156 pKa = 11.84 HH157 pKa = 6.27 CAPRR161 pKa = 11.84 WVTRR165 pKa = 11.84 AAXLQGIAIGAVIALLGAVIVDD187 pKa = 3.22 QXRR190 pKa = 11.84 SNRR193 pKa = 11.84 EE194 pKa = 3.45 QFVLLFGVSLLFTLATLAAGWVALRR219 pKa = 11.84 LPACSTEE226 pKa = 3.85 DD227 pKa = 2.93 RR228 pKa = 11.84 XRR230 pKa = 11.84 AALGFPARR238 pKa = 11.84 NVAVATLAATSVLGKK253 pKa = 10.24 AAVVTFIAVLFTTQLTLLLSLALWLRR279 pKa = 11.84 KK280 pKa = 9.2 RR281 pKa = 11.84 AA282 pKa = 3.58
Molecular weight: 29.38 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.352
IPC2_protein 9.487
IPC_protein 10.452
Toseland 10.657
ProMoST 10.452
Dawson 10.745
Bjellqvist 10.511
Wikipedia 10.994
Rodwell 10.804
Grimsley 10.789
Solomon 10.921
Lehninger 10.877
Nozaki 10.672
DTASelect 10.496
Thurlkill 10.657
EMBOSS 11.082
Sillero 10.687
Patrickios 10.57
IPC_peptide 10.921
IPC2_peptide 9.823
IPC2.peptide.svr19 8.626
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2873
0
2873
878941
37
1957
305.9
33.3
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.749 ± 0.07
1.092 ± 0.018
5.284 ± 0.031
5.706 ± 0.042
3.799 ± 0.032
8.046 ± 0.044
2.215 ± 0.021
5.139 ± 0.029
3.825 ± 0.041
10.723 ± 0.061
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.429 ± 0.024
2.957 ± 0.029
5.016 ± 0.031
3.573 ± 0.025
6.826 ± 0.045
5.174 ± 0.032
4.893 ± 0.033
7.171 ± 0.041
1.414 ± 0.021
2.396 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here