Prochlorococcus phage P-HM1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Eurybiavirus; unclassified Eurybiavirus

Average proteome isoelectric point is 5.97

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 241 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|E3SMQ0|E3SMQ0_9CAUD Uncharacterized protein OS=Prochlorococcus phage P-HM1 OX=445700 GN=PHM1_069 PE=4 SV=1
MM1 pKa = 5.87TTFYY5 pKa = 11.0EE6 pKa = 4.81GDD8 pKa = 3.27QPLWQDD14 pKa = 3.17HH15 pKa = 7.03KK16 pKa = 11.31DD17 pKa = 4.0YY18 pKa = 11.41AWNEE22 pKa = 4.03YY23 pKa = 10.04KK24 pKa = 10.57HH25 pKa = 6.46LEE27 pKa = 3.79RR28 pKa = 11.84DD29 pKa = 3.4KK30 pKa = 11.48FEE32 pKa = 4.67EE33 pKa = 4.39LLDD36 pKa = 3.86MMIAAYY42 pKa = 8.95PDD44 pKa = 3.73HH45 pKa = 7.25EE46 pKa = 4.39LTEE49 pKa = 4.14WMKK52 pKa = 10.73RR53 pKa = 11.84GYY55 pKa = 10.64CMNEE59 pKa = 3.6GDD61 pKa = 4.02STISFSSLSGRR72 pKa = 11.84RR73 pKa = 11.84MMQWDD78 pKa = 3.4IDD80 pKa = 3.58HH81 pKa = 7.24WDD83 pKa = 3.6EE84 pKa = 4.77EE85 pKa = 4.55DD86 pKa = 3.72AEE88 pKa = 4.77SYY90 pKa = 11.5ALFYY94 pKa = 11.36EE95 pKa = 4.61EE96 pKa = 5.15TEE98 pKa = 4.13EE99 pKa = 5.81LDD101 pKa = 4.0WDD103 pKa = 4.32DD104 pKa = 6.09DD105 pKa = 3.93DD106 pKa = 3.92WW107 pKa = 6.58

Molecular weight: 13.2 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|E3SMR7|E3SMR7_9CAUD Baseplate wedge OS=Prochlorococcus phage P-HM1 OX=445700 GN=gp6 PE=4 SV=1
MM1 pKa = 8.17DD2 pKa = 3.78NTRR5 pKa = 11.84SIVPRR10 pKa = 11.84LGTRR14 pKa = 11.84QRR16 pKa = 11.84RR17 pKa = 11.84GIVAKK22 pKa = 10.51ANRR25 pKa = 11.84IVDD28 pKa = 3.99GKK30 pKa = 10.65RR31 pKa = 11.84NANIPVDD38 pKa = 4.01MSDD41 pKa = 3.2HH42 pKa = 6.69FYY44 pKa = 11.61DD45 pKa = 5.06HH46 pKa = 6.81GNEE49 pKa = 3.82YY50 pKa = 10.36CRR52 pKa = 11.84YY53 pKa = 9.79LITDD57 pKa = 3.75PRR59 pKa = 11.84SDD61 pKa = 3.31RR62 pKa = 11.84QGKK65 pKa = 8.28KK66 pKa = 9.61RR67 pKa = 11.84KK68 pKa = 9.09PFEE71 pKa = 3.91KK72 pKa = 10.1RR73 pKa = 11.84VV74 pKa = 3.28

Molecular weight: 8.71 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

241

0

241

59111

33

4446

245.3

27.22

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.809 ± 0.217

0.947 ± 0.074

6.544 ± 0.142

5.737 ± 0.342

4.28 ± 0.097

7.682 ± 0.387

1.682 ± 0.113

6.302 ± 0.143

5.99 ± 0.433

7.056 ± 0.192

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.135 ± 0.204

5.921 ± 0.229

3.789 ± 0.108

3.712 ± 0.083

3.862 ± 0.14

7.276 ± 0.285

8.154 ± 0.509

6.709 ± 0.168

1.149 ± 0.098

4.263 ± 0.14
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski