Amino acid dipepetide frequency for Prochlorococcus phage P-HM1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.141AlaAla: 6.141 ± 0.462
0.558AlaCys: 0.558 ± 0.088
4.094AlaAsp: 4.094 ± 0.258
3.265AlaGlu: 3.265 ± 0.259
2.571AlaPhe: 2.571 ± 0.216
5.549AlaGly: 5.549 ± 0.509
1.015AlaHis: 1.015 ± 0.137
4.179AlaIle: 4.179 ± 0.31
3.857AlaLys: 3.857 ± 0.379
4.432AlaLeu: 4.432 ± 0.365
1.438AlaMet: 1.438 ± 0.165
4.483AlaAsn: 4.483 ± 0.444
2.385AlaPro: 2.385 ± 0.238
2.436AlaGln: 2.436 ± 0.229
2.47AlaArg: 2.47 ± 0.166
5.549AlaSer: 5.549 ± 0.527
6.378AlaThr: 6.378 ± 0.719
4.263AlaVal: 4.263 ± 0.324
0.575AlaTrp: 0.575 ± 0.108
2.453AlaTyr: 2.453 ± 0.207
0.0AlaXaa: 0.0 ± 0.0
Cys
0.711CysAla: 0.711 ± 0.076
0.135CysCys: 0.135 ± 0.05
0.778CysAsp: 0.778 ± 0.145
0.592CysGlu: 0.592 ± 0.118
0.423CysPhe: 0.423 ± 0.086
0.423CysGly: 0.423 ± 0.1
0.288CysHis: 0.288 ± 0.072
0.609CysIle: 0.609 ± 0.101
0.423CysLys: 0.423 ± 0.103
0.643CysLeu: 0.643 ± 0.113
0.237CysMet: 0.237 ± 0.068
0.508CysAsn: 0.508 ± 0.113
0.524CysPro: 0.524 ± 0.094
0.389CysGln: 0.389 ± 0.067
0.406CysArg: 0.406 ± 0.104
0.457CysSer: 0.457 ± 0.103
0.761CysThr: 0.761 ± 0.114
0.626CysVal: 0.626 ± 0.106
0.085CysTrp: 0.085 ± 0.039
0.457CysTyr: 0.457 ± 0.11
0.0CysXaa: 0.0 ± 0.0
Asp
4.872AspAla: 4.872 ± 0.34
0.677AspCys: 0.677 ± 0.122
4.297AspAsp: 4.297 ± 0.349
4.077AspGlu: 4.077 ± 0.368
2.893AspPhe: 2.893 ± 0.192
5.126AspGly: 5.126 ± 0.346
1.218AspHis: 1.218 ± 0.197
4.196AspIle: 4.196 ± 0.26
3.908AspLys: 3.908 ± 0.448
4.652AspLeu: 4.652 ± 0.358
1.709AspMet: 1.709 ± 0.255
3.722AspAsn: 3.722 ± 0.277
3.13AspPro: 3.13 ± 0.27
1.979AspGln: 1.979 ± 0.217
2.402AspArg: 2.402 ± 0.199
4.212AspSer: 4.212 ± 0.261
4.415AspThr: 4.415 ± 0.298
3.942AspVal: 3.942 ± 0.258
0.812AspTrp: 0.812 ± 0.134
3.181AspTyr: 3.181 ± 0.267
0.0AspXaa: 0.0 ± 0.0
Glu
3.333GluAla: 3.333 ± 0.243
0.491GluCys: 0.491 ± 0.093
3.773GluAsp: 3.773 ± 0.35
4.382GluGlu: 4.382 ± 0.475
2.707GluPhe: 2.707 ± 0.202
3.722GluGly: 3.722 ± 0.295
1.32GluHis: 1.32 ± 0.183
3.722GluIle: 3.722 ± 0.337
3.756GluLys: 3.756 ± 0.479
4.957GluLeu: 4.957 ± 0.426
1.556GluMet: 1.556 ± 0.268
2.977GluAsn: 2.977 ± 0.298
1.658GluPro: 1.658 ± 0.17
2.368GluGln: 2.368 ± 0.227
2.267GluArg: 2.267 ± 0.235
3.333GluSer: 3.333 ± 0.28
3.688GluThr: 3.688 ± 0.242
3.942GluVal: 3.942 ± 0.286
0.897GluTrp: 0.897 ± 0.149
2.521GluTyr: 2.521 ± 0.242
0.0GluXaa: 0.0 ± 0.0
Phe
2.639PheAla: 2.639 ± 0.208
0.524PheCys: 0.524 ± 0.093
3.197PheAsp: 3.197 ± 0.228
2.605PheGlu: 2.605 ± 0.236
1.692PhePhe: 1.692 ± 0.182
2.825PheGly: 2.825 ± 0.25
0.761PheHis: 0.761 ± 0.125
2.301PheIle: 2.301 ± 0.183
2.571PheLys: 2.571 ± 0.26
3.299PheLeu: 3.299 ± 0.27
0.897PheMet: 0.897 ± 0.156
2.487PheAsn: 2.487 ± 0.206
1.573PhePro: 1.573 ± 0.176
1.489PheGln: 1.489 ± 0.161
1.607PheArg: 1.607 ± 0.191
3.265PheSer: 3.265 ± 0.25
3.536PheThr: 3.536 ± 0.308
3.147PheVal: 3.147 ± 0.251
0.423PheTrp: 0.423 ± 0.108
1.962PheTyr: 1.962 ± 0.179
0.0PheXaa: 0.0 ± 0.0
Gly
5.634GlyAla: 5.634 ± 0.567
0.66GlyCys: 0.66 ± 0.103
5.126GlyAsp: 5.126 ± 0.413
3.434GlyGlu: 3.434 ± 0.212
2.774GlyPhe: 2.774 ± 0.232
7.934GlyGly: 7.934 ± 0.963
0.897GlyHis: 0.897 ± 0.124
4.585GlyIle: 4.585 ± 0.244
3.942GlyLys: 3.942 ± 0.428
4.737GlyLeu: 4.737 ± 0.309
1.421GlyMet: 1.421 ± 0.205
5.177GlyAsn: 5.177 ± 0.598
1.286GlyPro: 1.286 ± 0.155
2.91GlyGln: 2.91 ± 0.311
2.555GlyArg: 2.555 ± 0.21
7.122GlySer: 7.122 ± 0.828
7.833GlyThr: 7.833 ± 0.955
4.872GlyVal: 4.872 ± 0.394
0.812GlyTrp: 0.812 ± 0.116
3.113GlyTyr: 3.113 ± 0.313
0.0GlyXaa: 0.0 ± 0.0
His
0.711HisAla: 0.711 ± 0.122
0.22HisCys: 0.22 ± 0.065
0.829HisAsp: 0.829 ± 0.153
1.184HisGlu: 1.184 ± 0.153
0.863HisPhe: 0.863 ± 0.139
1.235HisGly: 1.235 ± 0.174
0.44HisHis: 0.44 ± 0.105
1.235HisIle: 1.235 ± 0.156
0.914HisLys: 0.914 ± 0.148
1.15HisLeu: 1.15 ± 0.181
0.474HisMet: 0.474 ± 0.106
0.812HisAsn: 0.812 ± 0.104
1.066HisPro: 1.066 ± 0.164
0.474HisGln: 0.474 ± 0.086
0.677HisArg: 0.677 ± 0.127
1.083HisSer: 1.083 ± 0.161
1.353HisThr: 1.353 ± 0.168
0.863HisVal: 0.863 ± 0.131
0.355HisTrp: 0.355 ± 0.089
0.88HisTyr: 0.88 ± 0.144
0.0HisXaa: 0.0 ± 0.0
Ile
3.959IleAla: 3.959 ± 0.205
0.491IleCys: 0.491 ± 0.104
4.652IleAsp: 4.652 ± 0.275
4.077IleGlu: 4.077 ± 0.273
2.267IlePhe: 2.267 ± 0.195
4.196IleGly: 4.196 ± 0.266
1.015IleHis: 1.015 ± 0.15
3.857IleIle: 3.857 ± 0.31
3.891IleLys: 3.891 ± 0.36
4.026IleLeu: 4.026 ± 0.273
1.049IleMet: 1.049 ± 0.185
3.908IleAsn: 3.908 ± 0.285
2.977IlePro: 2.977 ± 0.257
2.216IleGln: 2.216 ± 0.196
2.673IleArg: 2.673 ± 0.215
4.686IleSer: 4.686 ± 0.299
5.667IleThr: 5.667 ± 0.522
4.331IleVal: 4.331 ± 0.286
0.541IleTrp: 0.541 ± 0.109
2.538IleTyr: 2.538 ± 0.232
0.0IleXaa: 0.0 ± 0.0
Lys
3.451LysAla: 3.451 ± 0.309
0.66LysCys: 0.66 ± 0.11
3.451LysAsp: 3.451 ± 0.369
4.009LysGlu: 4.009 ± 0.494
2.571LysPhe: 2.571 ± 0.247
3.959LysGly: 3.959 ± 0.363
1.15LysHis: 1.15 ± 0.199
3.79LysIle: 3.79 ± 0.36
5.38LysLys: 5.38 ± 0.65
4.517LysLeu: 4.517 ± 0.383
1.726LysMet: 1.726 ± 0.267
3.079LysAsn: 3.079 ± 0.314
2.013LysPro: 2.013 ± 0.242
2.03LysGln: 2.03 ± 0.249
2.622LysArg: 2.622 ± 0.331
3.925LysSer: 3.925 ± 0.342
3.553LysThr: 3.553 ± 0.299
4.382LysVal: 4.382 ± 0.332
0.897LysTrp: 0.897 ± 0.143
2.741LysTyr: 2.741 ± 0.284
0.0LysXaa: 0.0 ± 0.0
Leu
4.652LeuAla: 4.652 ± 0.33
0.897LeuCys: 0.897 ± 0.174
6.023LeuAsp: 6.023 ± 0.362
4.551LeuGlu: 4.551 ± 0.398
2.588LeuPhe: 2.588 ± 0.284
4.415LeuGly: 4.415 ± 0.353
1.167LeuHis: 1.167 ± 0.184
4.128LeuIle: 4.128 ± 0.29
4.872LeuLys: 4.872 ± 0.405
4.703LeuLeu: 4.703 ± 0.42
1.37LeuMet: 1.37 ± 0.201
4.415LeuAsn: 4.415 ± 0.3
2.487LeuPro: 2.487 ± 0.233
2.69LeuGln: 2.69 ± 0.221
3.164LeuArg: 3.164 ± 0.223
5.194LeuSer: 5.194 ± 0.285
5.058LeuThr: 5.058 ± 0.393
4.517LeuVal: 4.517 ± 0.305
0.711LeuTrp: 0.711 ± 0.112
2.961LeuTyr: 2.961 ± 0.287
0.0LeuXaa: 0.0 ± 0.0
Met
1.523MetAla: 1.523 ± 0.173
0.305MetCys: 0.305 ± 0.087
1.472MetAsp: 1.472 ± 0.207
1.269MetGlu: 1.269 ± 0.178
0.914MetPhe: 0.914 ± 0.14
1.117MetGly: 1.117 ± 0.164
0.423MetHis: 0.423 ± 0.098
1.336MetIle: 1.336 ± 0.164
1.793MetLys: 1.793 ± 0.261
1.286MetLeu: 1.286 ± 0.176
0.744MetMet: 0.744 ± 0.17
1.1MetAsn: 1.1 ± 0.173
0.846MetPro: 0.846 ± 0.171
0.93MetGln: 0.93 ± 0.16
0.863MetArg: 0.863 ± 0.139
1.675MetSer: 1.675 ± 0.248
1.709MetThr: 1.709 ± 0.211
1.201MetVal: 1.201 ± 0.143
0.186MetTrp: 0.186 ± 0.052
0.66MetTyr: 0.66 ± 0.131
0.0MetXaa: 0.0 ± 0.0
Asn
4.432AsnAla: 4.432 ± 0.405
0.44AsnCys: 0.44 ± 0.097
3.096AsnAsp: 3.096 ± 0.226
3.045AsnGlu: 3.045 ± 0.239
2.673AsnPhe: 2.673 ± 0.174
5.346AsnGly: 5.346 ± 0.55
0.914AsnHis: 0.914 ± 0.129
4.06AsnIle: 4.06 ± 0.255
3.197AsnLys: 3.197 ± 0.313
4.534AsnLeu: 4.534 ± 0.252
1.1AsnMet: 1.1 ± 0.134
3.79AsnAsn: 3.79 ± 0.366
2.791AsnPro: 2.791 ± 0.217
2.182AsnGln: 2.182 ± 0.198
2.165AsnArg: 2.165 ± 0.191
3.806AsnSer: 3.806 ± 0.351
4.28AsnThr: 4.28 ± 0.409
4.162AsnVal: 4.162 ± 0.333
0.744AsnTrp: 0.744 ± 0.103
2.453AsnTyr: 2.453 ± 0.249
0.0AsnXaa: 0.0 ± 0.0
Pro
2.25ProAla: 2.25 ± 0.23
0.271ProCys: 0.271 ± 0.075
2.673ProAsp: 2.673 ± 0.273
2.521ProGlu: 2.521 ± 0.265
1.692ProPhe: 1.692 ± 0.175
2.199ProGly: 2.199 ± 0.22
0.711ProHis: 0.711 ± 0.137
2.047ProIle: 2.047 ± 0.231
2.047ProLys: 2.047 ± 0.271
2.436ProLeu: 2.436 ± 0.18
0.626ProMet: 0.626 ± 0.12
2.284ProAsn: 2.284 ± 0.168
1.523ProPro: 1.523 ± 0.226
1.303ProGln: 1.303 ± 0.16
1.303ProArg: 1.303 ± 0.147
2.588ProSer: 2.588 ± 0.225
3.722ProThr: 3.722 ± 0.321
3.282ProVal: 3.282 ± 0.242
0.558ProTrp: 0.558 ± 0.114
1.861ProTyr: 1.861 ± 0.203
0.0ProXaa: 0.0 ± 0.0
Gln
2.132GlnAla: 2.132 ± 0.166
0.271GlnCys: 0.271 ± 0.066
1.946GlnAsp: 1.946 ± 0.182
2.149GlnGlu: 2.149 ± 0.229
1.895GlnPhe: 1.895 ± 0.15
2.605GlnGly: 2.605 ± 0.29
0.626GlnHis: 0.626 ± 0.11
2.69GlnIle: 2.69 ± 0.251
2.25GlnLys: 2.25 ± 0.225
3.35GlnLeu: 3.35 ± 0.283
0.795GlnMet: 0.795 ± 0.144
2.301GlnAsn: 2.301 ± 0.198
1.472GlnPro: 1.472 ± 0.175
1.556GlnGln: 1.556 ± 0.246
1.455GlnArg: 1.455 ± 0.164
2.673GlnSer: 2.673 ± 0.204
2.368GlnThr: 2.368 ± 0.219
2.385GlnVal: 2.385 ± 0.224
0.592GlnTrp: 0.592 ± 0.139
1.607GlnTyr: 1.607 ± 0.188
0.0GlnXaa: 0.0 ± 0.0
Arg
2.47ArgAla: 2.47 ± 0.258
0.44ArgCys: 0.44 ± 0.088
2.487ArgAsp: 2.487 ± 0.194
2.368ArgGlu: 2.368 ± 0.276
2.013ArgPhe: 2.013 ± 0.166
2.538ArgGly: 2.538 ± 0.219
0.524ArgHis: 0.524 ± 0.112
2.842ArgIle: 2.842 ± 0.233
2.656ArgLys: 2.656 ± 0.381
2.893ArgLeu: 2.893 ± 0.244
0.914ArgMet: 0.914 ± 0.143
1.827ArgAsn: 1.827 ± 0.172
1.32ArgPro: 1.32 ± 0.168
1.421ArgGln: 1.421 ± 0.189
2.064ArgArg: 2.064 ± 0.223
2.588ArgSer: 2.588 ± 0.232
2.335ArgThr: 2.335 ± 0.257
2.758ArgVal: 2.758 ± 0.2
0.44ArgTrp: 0.44 ± 0.079
1.726ArgTyr: 1.726 ± 0.194
0.0ArgXaa: 0.0 ± 0.0
Ser
5.16SerAla: 5.16 ± 0.34
0.711SerCys: 0.711 ± 0.12
4.365SerAsp: 4.365 ± 0.237
3.13SerGlu: 3.13 ± 0.228
3.265SerPhe: 3.265 ± 0.249
8.239SerGly: 8.239 ± 0.886
1.015SerHis: 1.015 ± 0.132
4.652SerIle: 4.652 ± 0.339
3.536SerLys: 3.536 ± 0.314
4.923SerLeu: 4.923 ± 0.265
1.32SerMet: 1.32 ± 0.152
4.382SerAsn: 4.382 ± 0.509
2.774SerPro: 2.774 ± 0.241
2.758SerGln: 2.758 ± 0.181
2.335SerArg: 2.335 ± 0.219
5.786SerSer: 5.786 ± 0.496
6.023SerThr: 6.023 ± 0.619
4.805SerVal: 4.805 ± 0.399
0.795SerTrp: 0.795 ± 0.103
2.791SerTyr: 2.791 ± 0.242
0.0SerXaa: 0.0 ± 0.0
Thr
5.82ThrAla: 5.82 ± 0.66
0.558ThrCys: 0.558 ± 0.115
4.669ThrAsp: 4.669 ± 0.365
3.536ThrGlu: 3.536 ± 0.248
4.06ThrPhe: 4.06 ± 0.357
7.105ThrGly: 7.105 ± 0.866
1.167ThrHis: 1.167 ± 0.154
5.583ThrIle: 5.583 ± 0.544
3.434ThrLys: 3.434 ± 0.302
5.803ThrLeu: 5.803 ± 0.401
1.489ThrMet: 1.489 ± 0.165
4.923ThrAsn: 4.923 ± 0.565
3.62ThrPro: 3.62 ± 0.314
3.045ThrGln: 3.045 ± 0.238
2.741ThrArg: 2.741 ± 0.186
6.057ThrSer: 6.057 ± 0.626
7.714ThrThr: 7.714 ± 0.925
5.921ThrVal: 5.921 ± 0.637
0.88ThrTrp: 0.88 ± 0.12
3.417ThrTyr: 3.417 ± 0.448
0.0ThrXaa: 0.0 ± 0.0
Val
5.025ValAla: 5.025 ± 0.398
0.558ValCys: 0.558 ± 0.122
4.5ValAsp: 4.5 ± 0.256
4.009ValGlu: 4.009 ± 0.29
2.724ValPhe: 2.724 ± 0.256
5.397ValGly: 5.397 ± 0.428
0.914ValHis: 0.914 ± 0.12
3.739ValIle: 3.739 ± 0.209
4.162ValLys: 4.162 ± 0.328
4.314ValLeu: 4.314 ± 0.315
1.269ValMet: 1.269 ± 0.176
3.587ValAsn: 3.587 ± 0.279
2.588ValPro: 2.588 ± 0.218
2.673ValGln: 2.673 ± 0.208
2.199ValArg: 2.199 ± 0.159
5.228ValSer: 5.228 ± 0.448
6.75ValThr: 6.75 ± 0.757
4.28ValVal: 4.28 ± 0.356
0.541ValTrp: 0.541 ± 0.109
2.639ValTyr: 2.639 ± 0.199
0.0ValXaa: 0.0 ± 0.0
Trp
0.761TrpAla: 0.761 ± 0.11
0.203TrpCys: 0.203 ± 0.061
0.744TrpAsp: 0.744 ± 0.149
0.846TrpGlu: 0.846 ± 0.13
0.491TrpPhe: 0.491 ± 0.112
0.524TrpGly: 0.524 ± 0.08
0.406TrpHis: 0.406 ± 0.09
0.643TrpIle: 0.643 ± 0.115
0.761TrpLys: 0.761 ± 0.139
0.778TrpLeu: 0.778 ± 0.121
0.355TrpMet: 0.355 ± 0.085
0.711TrpAsn: 0.711 ± 0.096
0.169TrpPro: 0.169 ± 0.055
0.541TrpGln: 0.541 ± 0.117
0.575TrpArg: 0.575 ± 0.103
0.778TrpSer: 0.778 ± 0.125
0.846TrpThr: 0.846 ± 0.122
0.744TrpVal: 0.744 ± 0.138
0.135TrpTrp: 0.135 ± 0.047
0.474TrpTyr: 0.474 ± 0.089
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.419TyrAla: 2.419 ± 0.216
0.406TyrCys: 0.406 ± 0.083
3.265TyrAsp: 3.265 ± 0.281
2.318TyrGlu: 2.318 ± 0.274
1.726TyrPhe: 1.726 ± 0.172
2.368TyrGly: 2.368 ± 0.222
0.846TyrHis: 0.846 ± 0.138
2.825TyrIle: 2.825 ± 0.218
2.555TyrLys: 2.555 ± 0.227
3.13TyrLeu: 3.13 ± 0.233
0.846TyrMet: 0.846 ± 0.164
2.741TyrAsn: 2.741 ± 0.221
1.692TyrPro: 1.692 ± 0.251
1.726TyrGln: 1.726 ± 0.187
2.081TyrArg: 2.081 ± 0.206
2.758TyrSer: 2.758 ± 0.201
3.553TyrThr: 3.553 ± 0.324
2.673TyrVal: 2.673 ± 0.201
0.508TyrTrp: 0.508 ± 0.097
2.199TyrTyr: 2.199 ± 0.205
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 241 proteins (59111 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski