Salmonella phage PVPSE1
Average proteome isoelectric point is 6.32
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 244 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|G3BM51|G3BM51_9CAUD Uncharacterized protein 185 OS=Salmonella phage PVPSE1 OX=889338 GN=185 PE=4 SV=1
MM1 pKa = 7.83 EE2 pKa = 4.78 LTDD5 pKa = 5.14 LYY7 pKa = 11.14 SDD9 pKa = 3.62 APIRR13 pKa = 11.84 LSPEE17 pKa = 3.66 EE18 pKa = 4.15 YY19 pKa = 10.49 KK20 pKa = 11.01 NSEE23 pKa = 4.13 LVKK26 pKa = 10.42 HH27 pKa = 5.19 VVAMEE32 pKa = 3.96 FEE34 pKa = 4.27 FFEE37 pKa = 4.35 YY38 pKa = 10.73 LADD41 pKa = 4.78 FDD43 pKa = 6.83 DD44 pKa = 5.41 DD45 pKa = 4.56 FNSDD49 pKa = 3.43 GLYY52 pKa = 10.72 GAA54 pKa = 5.36
Molecular weight: 6.29 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.766
IPC2_protein 3.821
IPC_protein 3.732
Toseland 3.541
ProMoST 3.897
Dawson 3.719
Bjellqvist 3.884
Wikipedia 3.656
Rodwell 3.567
Grimsley 3.465
Solomon 3.694
Lehninger 3.643
Nozaki 3.859
DTASelect 4.012
Thurlkill 3.605
EMBOSS 3.668
Sillero 3.846
Patrickios 1.863
IPC_peptide 3.694
IPC2_peptide 3.821
IPC2.peptide.svr19 3.789
Protein with the highest isoelectric point:
>tr|G3BM69|G3BM69_9CAUD Uncharacterized protein 203 OS=Salmonella phage PVPSE1 OX=889338 GN=203 PE=4 SV=1
MM1 pKa = 7.53 KK2 pKa = 10.48 NYY4 pKa = 10.0 FNSRR8 pKa = 11.84 ALARR12 pKa = 11.84 TAAKK16 pKa = 10.68 ANGGKK21 pKa = 10.55 VIDD24 pKa = 4.7 NGTAAPAGKK33 pKa = 9.06 RR34 pKa = 11.84 WQVIPGVMVEE44 pKa = 4.99 AIQDD48 pKa = 3.72 TADD51 pKa = 2.94 QRR53 pKa = 11.84 AAMEE57 pKa = 4.28 SAPALVVTLEE67 pKa = 3.85 IKK69 pKa = 10.2 AGYY72 pKa = 9.74 RR73 pKa = 11.84 NRR75 pKa = 11.84 QAARR79 pKa = 11.84 VSYY82 pKa = 10.98 VHH84 pKa = 7.27 DD85 pKa = 4.8 ANGKK89 pKa = 8.24 PIPVTHH95 pKa = 6.8 KK96 pKa = 9.92 RR97 pKa = 11.84 SQIAARR103 pKa = 11.84 LAAHH107 pKa = 6.73 MARR110 pKa = 4.5
Molecular weight: 11.81 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.314
IPC2_protein 9.897
IPC_protein 10.774
Toseland 10.95
ProMoST 11.023
Dawson 11.023
Bjellqvist 10.745
Wikipedia 11.257
Rodwell 11.257
Grimsley 11.067
Solomon 11.184
Lehninger 11.14
Nozaki 10.921
DTASelect 10.745
Thurlkill 10.935
EMBOSS 11.359
Sillero 10.95
Patrickios 11.008
IPC_peptide 11.184
IPC2_peptide 9.575
IPC2.peptide.svr19 8.611
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
244
0
244
44306
38
929
181.6
20.46
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.153 ± 0.2
1.239 ± 0.077
6.577 ± 0.148
6.609 ± 0.179
4.239 ± 0.122
7.403 ± 0.306
1.79 ± 0.089
5.688 ± 0.133
6.875 ± 0.2
7.886 ± 0.157
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.954 ± 0.103
4.724 ± 0.13
3.74 ± 0.142
3.53 ± 0.115
4.875 ± 0.147
5.848 ± 0.169
5.909 ± 0.217
7.229 ± 0.157
1.756 ± 0.09
3.977 ± 0.111
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here