Vibrio hepatarius
Average proteome isoelectric point is 5.99
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4313 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0M0HZ37|A0A0M0HZ37_9VIBR 2-octaprenyl-3-methyl-6-methoxy-1 4-benzoquinol hydroxylase OS=Vibrio hepatarius OX=171383 GN=ubiF PE=3 SV=1
MM1 pKa = 7.04 TVQRR5 pKa = 11.84 APIEE9 pKa = 4.08 MTFEE13 pKa = 3.44 EE14 pKa = 4.93 WFEE17 pKa = 4.07 KK18 pKa = 10.18 FKK20 pKa = 10.99 PVANPTGDD28 pKa = 3.35 GFVQVDD34 pKa = 3.72 DD35 pKa = 3.72 VCYY38 pKa = 10.89 VFGLHH43 pKa = 6.71 GADD46 pKa = 3.45 LSKK49 pKa = 11.24 VQAADD54 pKa = 4.01 PNCVWTLIEE63 pKa = 5.42 SDD65 pKa = 5.79 DD66 pKa = 3.98 VDD68 pKa = 4.78 CDD70 pKa = 4.34 EE71 pKa = 6.51 DD72 pKa = 5.86 DD73 pKa = 3.81 EE74 pKa = 7.27 DD75 pKa = 5.63 YY76 pKa = 10.37 DD77 pKa = 4.27 TVLLISDD84 pKa = 4.54 GYY86 pKa = 11.0 HH87 pKa = 5.23 RR88 pKa = 11.84 VNRR91 pKa = 11.84 MGHH94 pKa = 6.89 FITEE98 pKa = 4.14 VPADD102 pKa = 3.63 PEE104 pKa = 4.17 SFYY107 pKa = 10.92 EE108 pKa = 3.92 ISYY111 pKa = 10.56 DD112 pKa = 3.36
Molecular weight: 12.78 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.705
IPC2_protein 3.795
IPC_protein 3.783
Toseland 3.567
ProMoST 3.897
Dawson 3.783
Bjellqvist 3.973
Wikipedia 3.719
Rodwell 3.605
Grimsley 3.478
Solomon 3.77
Lehninger 3.719
Nozaki 3.897
DTASelect 4.126
Thurlkill 3.63
EMBOSS 3.732
Sillero 3.91
Patrickios 0.998
IPC_peptide 3.757
IPC2_peptide 3.884
IPC2.peptide.svr19 3.828
Protein with the highest isoelectric point:
>tr|A0A0M0I384|A0A0M0I384_9VIBR ABC transporter permease OS=Vibrio hepatarius OX=171383 GN=AKJ31_05990 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.5 RR3 pKa = 11.84 TFQPTVLKK11 pKa = 10.46 RR12 pKa = 11.84 KK13 pKa = 7.65 RR14 pKa = 11.84 THH16 pKa = 5.89 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.44 NGRR28 pKa = 11.84 KK29 pKa = 9.39 VINARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.22 GRR39 pKa = 11.84 ARR41 pKa = 11.84 LSKK44 pKa = 10.83
Molecular weight: 5.18 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.517
IPC2_protein 11.213
IPC_protein 12.793
Toseland 12.969
ProMoST 13.451
Dawson 12.969
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.705
Grimsley 12.998
Solomon 13.451
Lehninger 13.364
Nozaki 12.969
DTASelect 12.954
Thurlkill 12.969
EMBOSS 13.466
Sillero 12.969
Patrickios 12.427
IPC_peptide 13.466
IPC2_peptide 12.442
IPC2.peptide.svr19 9.142
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4313
0
4313
1386379
37
3656
321.4
35.71
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.68 ± 0.042
1.028 ± 0.013
5.529 ± 0.032
6.573 ± 0.036
4.108 ± 0.024
6.81 ± 0.034
2.179 ± 0.019
6.184 ± 0.026
5.33 ± 0.033
10.32 ± 0.047
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.668 ± 0.022
4.114 ± 0.024
3.893 ± 0.025
4.578 ± 0.029
4.508 ± 0.03
6.66 ± 0.032
5.278 ± 0.029
7.253 ± 0.034
1.248 ± 0.012
3.061 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here