Paracoccus zhejiangensis
Average proteome isoelectric point is 6.19
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4274 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2H5F496|A0A2H5F496_9RHOB Uncharacterized protein OS=Paracoccus zhejiangensis OX=1077935 GN=CX676_14425 PE=4 SV=1
MM1 pKa = 7.56 KK2 pKa = 10.26 LAIAAVAATLTAGSAFAGGYY22 pKa = 6.21 TSPVVEE28 pKa = 4.7 TPVIAPVAPVAVDD41 pKa = 5.07 AGTDD45 pKa = 3.12 WTGFYY50 pKa = 10.75 AGLQYY55 pKa = 11.26 GQGNAEE61 pKa = 4.11 ASLGGDD67 pKa = 3.09 NVEE70 pKa = 4.85 SDD72 pKa = 3.29 FDD74 pKa = 4.23 AYY76 pKa = 10.38 GVHH79 pKa = 6.64 GGYY82 pKa = 9.68 NHH84 pKa = 7.79 DD85 pKa = 3.86 FGNYY89 pKa = 7.67 VLGGEE94 pKa = 4.28 LDD96 pKa = 3.89 YY97 pKa = 11.94 NKK99 pKa = 10.0 VDD101 pKa = 5.38 LDD103 pKa = 4.54 DD104 pKa = 5.57 ADD106 pKa = 4.3 GDD108 pKa = 3.92 ADD110 pKa = 3.86 LWRR113 pKa = 11.84 LRR115 pKa = 11.84 GRR117 pKa = 11.84 AGYY120 pKa = 11.03 DD121 pKa = 2.81 MGKK124 pKa = 9.73 FLPYY128 pKa = 8.71 VTLGAAHH135 pKa = 7.3 ISGDD139 pKa = 3.61 NDD141 pKa = 3.4 LSEE144 pKa = 4.29 TDD146 pKa = 3.27 VTYY149 pKa = 10.66 GIGGEE154 pKa = 4.2 YY155 pKa = 9.7 MVTDD159 pKa = 4.5 RR160 pKa = 11.84 FTVGAEE166 pKa = 3.74 YY167 pKa = 9.85 TKK169 pKa = 10.7 QDD171 pKa = 3.3 FSDD174 pKa = 3.71 VQNVDD179 pKa = 3.77 GLDD182 pKa = 3.7 LDD184 pKa = 3.82 SDD186 pKa = 4.28 MVQVRR191 pKa = 11.84 ASFRR195 pKa = 11.84 FF196 pKa = 3.43
Molecular weight: 20.74 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.69
IPC2_protein 3.821
IPC_protein 3.846
Toseland 3.605
ProMoST 4.024
Dawson 3.846
Bjellqvist 3.999
Wikipedia 3.821
Rodwell 3.668
Grimsley 3.516
Solomon 3.846
Lehninger 3.795
Nozaki 3.961
DTASelect 4.266
Thurlkill 3.668
EMBOSS 3.821
Sillero 3.973
Patrickios 1.138
IPC_peptide 3.834
IPC2_peptide 3.935
IPC2.peptide.svr19 3.842
Protein with the highest isoelectric point:
>tr|A0A2H5F3C7|A0A2H5F3C7_9RHOB Glutathione S-transferase OS=Paracoccus zhejiangensis OX=1077935 GN=CX676_19340 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.61 RR3 pKa = 11.84 TFQPSNLVRR12 pKa = 11.84 ARR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.37 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.41 AGRR28 pKa = 11.84 LVLNRR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.7 GRR39 pKa = 11.84 KK40 pKa = 8.85 RR41 pKa = 11.84 LSAA44 pKa = 3.96
Molecular weight: 5.18 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.286
IPC_protein 12.881
Toseland 13.042
ProMoST 13.539
Dawson 13.042
Bjellqvist 13.042
Wikipedia 13.525
Rodwell 12.676
Grimsley 13.086
Solomon 13.539
Lehninger 13.437
Nozaki 13.042
DTASelect 13.042
Thurlkill 13.042
EMBOSS 13.539
Sillero 13.042
Patrickios 12.398
IPC_peptide 13.539
IPC2_peptide 12.53
IPC2.peptide.svr19 9.215
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4274
0
4274
1366512
30
3588
319.7
34.58
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.948 ± 0.046
0.799 ± 0.011
5.971 ± 0.03
5.715 ± 0.036
3.539 ± 0.025
9.051 ± 0.046
2.004 ± 0.019
5.187 ± 0.027
2.694 ± 0.032
10.333 ± 0.052
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.737 ± 0.02
2.44 ± 0.024
5.406 ± 0.028
3.267 ± 0.021
7.125 ± 0.046
4.989 ± 0.024
5.258 ± 0.037
6.97 ± 0.03
1.478 ± 0.014
2.088 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here