Bifidobacterium aemilianum

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium

Average proteome isoelectric point is 6.19

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1589 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A366K8Z5|A0A366K8Z5_9BIFI 4'-phosphopantetheinyl transferase OS=Bifidobacterium aemilianum OX=2493120 GN=CRD60_05190 PE=4 SV=1
MM1 pKa = 7.57TYY3 pKa = 10.6VIAQPCVDD11 pKa = 4.41VKK13 pKa = 11.37DD14 pKa = 3.89KK15 pKa = 11.56ACVDD19 pKa = 4.0EE20 pKa = 5.6CPVDD24 pKa = 4.25CIYY27 pKa = 10.75EE28 pKa = 4.5GKK30 pKa = 10.21RR31 pKa = 11.84SLYY34 pKa = 10.15INPNEE39 pKa = 4.37CVDD42 pKa = 4.29CGACEE47 pKa = 3.85PVCPVEE53 pKa = 4.7AIFYY57 pKa = 10.61EE58 pKa = 5.38DD59 pKa = 5.5DD60 pKa = 6.07LPDD63 pKa = 3.41DD64 pKa = 3.84WAWYY68 pKa = 10.2KK69 pKa = 11.0DD70 pKa = 3.58VADD73 pKa = 4.42SYY75 pKa = 11.1FAEE78 pKa = 4.77LGDD81 pKa = 4.75LGGAQAAGPSGKK93 pKa = 10.21DD94 pKa = 3.33PEE96 pKa = 4.63QVTALPPQGEE106 pKa = 4.24

Molecular weight: 11.5 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A366K8Z2|A0A366K8Z2_9BIFI DEAD_assoc domain-containing protein OS=Bifidobacterium aemilianum OX=2493120 GN=CRD60_03335 PE=4 SV=1
MM1 pKa = 7.44KK2 pKa = 9.6RR3 pKa = 11.84TFQPNNRR10 pKa = 11.84RR11 pKa = 11.84RR12 pKa = 11.84HH13 pKa = 4.79MKK15 pKa = 9.36HH16 pKa = 5.81GFRR19 pKa = 11.84ARR21 pKa = 11.84MRR23 pKa = 11.84TRR25 pKa = 11.84SGRR28 pKa = 11.84ALINRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.24GRR39 pKa = 11.84SVLSAA44 pKa = 3.74

Molecular weight: 5.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1589

0

1589

545325

24

3171

343.2

37.32

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.483 ± 0.072

0.893 ± 0.02

6.311 ± 0.054

5.351 ± 0.066

3.221 ± 0.043

8.552 ± 0.053

2.103 ± 0.031

5.142 ± 0.051

3.972 ± 0.054

9.671 ± 0.076

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.556 ± 0.027

2.931 ± 0.037

4.941 ± 0.047

4.503 ± 0.04

5.788 ± 0.062

6.834 ± 0.055

5.607 ± 0.061

7.209 ± 0.051

1.362 ± 0.028

2.571 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski