Cinnamomum micranthum f. kanehirae
Average proteome isoelectric point is 6.71
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 26414 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A443NYE3|A0A443NYE3_9MAGN Transposon protein putative CACTA En/Spm sub-class OS=Cinnamomum micranthum f. kanehirae OX=337451 GN=CKAN_01224800 PE=3 SV=1
MM1 pKa = 7.75 ASFFTDD7 pKa = 4.39 IISLDD12 pKa = 3.28 GWNDD16 pKa = 3.17 FSDD19 pKa = 3.75 PTRR22 pKa = 11.84 DD23 pKa = 3.57 QLSLVALVYY32 pKa = 10.53 EE33 pKa = 4.73 DD34 pKa = 3.21 RR35 pKa = 11.84 TVFYY39 pKa = 11.26 GEE41 pKa = 4.17 YY42 pKa = 9.96 KK43 pKa = 10.77 CIGEE47 pKa = 4.32 GANMTMRR54 pKa = 11.84 VPYY57 pKa = 10.3 AQGLNEE63 pKa = 4.17 TQASPFLDD71 pKa = 2.72 ISFINDD77 pKa = 3.43 QQWLQPYY84 pKa = 8.27 QQ85 pKa = 3.27
Molecular weight: 9.78 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.743
IPC2_protein 3.821
IPC_protein 3.745
Toseland 3.541
ProMoST 3.935
Dawson 3.757
Bjellqvist 3.923
Wikipedia 3.732
Rodwell 3.579
Grimsley 3.452
Solomon 3.719
Lehninger 3.681
Nozaki 3.897
DTASelect 4.113
Thurlkill 3.617
EMBOSS 3.732
Sillero 3.872
Patrickios 0.693
IPC_peptide 3.719
IPC2_peptide 3.834
IPC2.peptide.svr19 3.788
Protein with the highest isoelectric point:
>tr|A0A3S3NNY2|A0A3S3NNY2_9MAGN Transmembrane protein 18 OS=Cinnamomum micranthum f. kanehirae OX=337451 GN=CKAN_00053100 PE=3 SV=1
MM1 pKa = 7.32 IFSRR5 pKa = 11.84 PSLPRR10 pKa = 11.84 RR11 pKa = 11.84 VSRR14 pKa = 11.84 ARR16 pKa = 11.84 AFSRR20 pKa = 11.84 LLPPTSFQTPRR31 pKa = 11.84 PLL33 pKa = 4.65
Molecular weight: 3.84 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.462
IPC2_protein 11.008
IPC_protein 12.618
Toseland 12.778
ProMoST 13.276
Dawson 12.778
Bjellqvist 12.778
Wikipedia 13.261
Rodwell 12.281
Grimsley 12.822
Solomon 13.276
Lehninger 13.173
Nozaki 12.778
DTASelect 12.778
Thurlkill 12.778
EMBOSS 13.276
Sillero 12.778
Patrickios 12.047
IPC_peptide 13.276
IPC2_peptide 12.266
IPC2.peptide.svr19 9.153
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
26414
0
26414
11631408
17
7258
440.4
49.12
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.702 ± 0.014
1.904 ± 0.009
5.201 ± 0.01
6.431 ± 0.017
4.22 ± 0.01
6.715 ± 0.02
2.439 ± 0.007
5.394 ± 0.012
5.779 ± 0.015
9.955 ± 0.021
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.517 ± 0.006
4.29 ± 0.009
5.057 ± 0.017
3.626 ± 0.012
5.515 ± 0.018
8.996 ± 0.016
4.846 ± 0.009
6.366 ± 0.012
1.33 ± 0.005
2.716 ± 0.009
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here