Paracoccus aestuarii
Average proteome isoelectric point is 6.53
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3662 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A418ZV21|A0A418ZV21_9RHOB Phosphopentomutase OS=Paracoccus aestuarii OX=453842 GN=deoB PE=3 SV=1
MM1 pKa = 7.68 TEE3 pKa = 4.23 PLMEE7 pKa = 4.75 GAPLIAPSSTDD18 pKa = 3.0 HH19 pKa = 6.99 PLYY22 pKa = 10.62 EE23 pKa = 5.26 PIVEE27 pKa = 4.27 ACKK30 pKa = 9.49 TVYY33 pKa = 10.67 DD34 pKa = 4.01 PEE36 pKa = 5.2 IPVNIHH42 pKa = 6.91 DD43 pKa = 5.23 LGLIYY48 pKa = 10.15 TVEE51 pKa = 4.11 INDD54 pKa = 3.5 ASEE57 pKa = 3.89 VRR59 pKa = 11.84 VIMTLTAPGCPVAGEE74 pKa = 4.15 MPGWVADD81 pKa = 3.81 AVEE84 pKa = 4.48 AVPGVKK90 pKa = 9.81 QVDD93 pKa = 3.63 VEE95 pKa = 4.6 MTFQPQWGMDD105 pKa = 3.58 MMSDD109 pKa = 3.68 EE110 pKa = 4.89 ARR112 pKa = 11.84 LEE114 pKa = 4.14 LGFMM118 pKa = 4.54
Molecular weight: 12.86 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.772
IPC2_protein 3.884
IPC_protein 3.808
Toseland 3.63
ProMoST 3.948
Dawson 3.77
Bjellqvist 3.935
Wikipedia 3.681
Rodwell 3.643
Grimsley 3.541
Solomon 3.757
Lehninger 3.719
Nozaki 3.897
DTASelect 4.037
Thurlkill 3.668
EMBOSS 3.694
Sillero 3.923
Patrickios 1.863
IPC_peptide 3.757
IPC2_peptide 3.897
IPC2.peptide.svr19 3.826
Protein with the highest isoelectric point:
>tr|A0A419A1U2|A0A419A1U2_9RHOB Uncharacterized protein OS=Paracoccus aestuarii OX=453842 GN=D3P06_02480 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.61 RR3 pKa = 11.84 TFQPSNLVRR12 pKa = 11.84 ARR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.37 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.39 GGRR28 pKa = 11.84 LVLNRR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.7 GRR39 pKa = 11.84 KK40 pKa = 8.85 RR41 pKa = 11.84 LSAA44 pKa = 3.96
Molecular weight: 5.16 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.286
IPC_protein 12.881
Toseland 13.042
ProMoST 13.539
Dawson 13.042
Bjellqvist 13.042
Wikipedia 13.525
Rodwell 12.676
Grimsley 13.086
Solomon 13.539
Lehninger 13.437
Nozaki 13.042
DTASelect 13.042
Thurlkill 13.042
EMBOSS 13.539
Sillero 13.042
Patrickios 12.398
IPC_peptide 13.539
IPC2_peptide 12.53
IPC2.peptide.svr19 9.215
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3662
0
3662
1101469
28
1512
300.8
32.53
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.599 ± 0.066
0.821 ± 0.011
6.355 ± 0.038
5.143 ± 0.037
3.373 ± 0.026
8.996 ± 0.038
2.113 ± 0.022
5.007 ± 0.029
2.12 ± 0.032
10.421 ± 0.045
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.863 ± 0.021
2.12 ± 0.02
5.629 ± 0.031
3.326 ± 0.021
8.073 ± 0.046
4.528 ± 0.023
5.148 ± 0.025
6.975 ± 0.033
1.49 ± 0.018
1.9 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here