Scandinavium goeteborgense
Average proteome isoelectric point is 6.45
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4446 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4R6DP91|A0A4R6DP91_9ENTR Outer membrane autotransporter protein OS=Scandinavium goeteborgense OX=1851514 GN=EC847_1401 PE=4 SV=1
MM1 pKa = 7.27 RR2 pKa = 11.84 TMVQDD7 pKa = 3.75 EE8 pKa = 4.6 LCDD11 pKa = 3.82 YY12 pKa = 11.12 DD13 pKa = 5.4 IDD15 pKa = 5.16 DD16 pKa = 4.94 EE17 pKa = 5.19 IYY19 pKa = 10.72 FNKK22 pKa = 10.16 EE23 pKa = 3.65 DD24 pKa = 4.82 LKK26 pKa = 11.52 DD27 pKa = 3.96 GDD29 pKa = 4.41 DD30 pKa = 4.32 SYY32 pKa = 12.07 LGAQDD37 pKa = 3.98 QNGDD41 pKa = 3.39 NTGTIHH47 pKa = 6.53 ICWSSLIFMRR57 pKa = 11.84 IGVV60 pKa = 3.69
Molecular weight: 6.92 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.731
IPC2_protein 3.77
IPC_protein 3.732
Toseland 3.503
ProMoST 3.935
Dawson 3.745
Bjellqvist 3.91
Wikipedia 3.745
Rodwell 3.554
Grimsley 3.427
Solomon 3.719
Lehninger 3.681
Nozaki 3.884
DTASelect 4.151
Thurlkill 3.592
EMBOSS 3.745
Sillero 3.859
Patrickios 1.901
IPC_peptide 3.719
IPC2_peptide 3.821
IPC2.peptide.svr19 3.782
Protein with the highest isoelectric point:
>tr|A0A4R6EMU1|A0A4R6EMU1_9ENTR Ditrans polycis-undecaprenyl-diphosphate synthase ((2E 6E)-farnesyl-diphosphate specific) OS=Scandinavium goeteborgense OX=1851514 GN=uppS PE=3 SV=1
MM1 pKa = 8.0 RR2 pKa = 11.84 ISCHH6 pKa = 5.74 NRR8 pKa = 11.84 VLLLLVLIALAAAALPFVNYY28 pKa = 10.31 APNRR32 pKa = 11.84 LVSGEE37 pKa = 4.34 SRR39 pKa = 11.84 QLWQIWTFPPALLLIVPLVFVGLSLLRR66 pKa = 11.84 GRR68 pKa = 11.84 GALALTLVLAQVLFILLLWSGGKK91 pKa = 9.65 AATTLAQSGSPLARR105 pKa = 11.84 TSPGSGLWLWLAVALLICSEE125 pKa = 4.71 AIRR128 pKa = 11.84 RR129 pKa = 11.84 LTQKK133 pKa = 10.79 SLWRR137 pKa = 11.84 WLLNAQIWIIPVALLLSGEE156 pKa = 4.4 LDD158 pKa = 3.4 SLSLMKK164 pKa = 10.4 EE165 pKa = 3.8 YY166 pKa = 10.71 FNRR169 pKa = 11.84 QDD171 pKa = 3.39 VFDD174 pKa = 4.5 SALQQHH180 pKa = 7.37 LILLFGTLLPGLLIGVPLGLWCWRR204 pKa = 11.84 HH205 pKa = 5.22 QGRR208 pKa = 11.84 QTRR211 pKa = 11.84 VFTVLNIIQTIPSVALFGLLIAPLAGLVKK240 pKa = 10.39 HH241 pKa = 6.22 FPWLSAWGIGGTGLTPALIALVLYY265 pKa = 10.73 ALLPLVRR272 pKa = 11.84 GVVAGMQQVAADD284 pKa = 3.91 VLEE287 pKa = 4.64 SASAMGMSQTQRR299 pKa = 11.84 FWKK302 pKa = 9.96 VQLPLALPVLLRR314 pKa = 11.84 SLRR317 pKa = 11.84 VVTVQTVGMAVVAALIGAGGFGALVFQGLLSSALDD352 pKa = 3.67 LVLLGVIPVIAMAVVVDD369 pKa = 4.44 ALFSLWIALLGGEE382 pKa = 4.37 AHH384 pKa = 7.38 DD385 pKa = 4.16
Molecular weight: 41.37 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.352
IPC2_protein 9.502
IPC_protein 10.35
Toseland 10.54
ProMoST 10.277
Dawson 10.643
Bjellqvist 10.394
Wikipedia 10.877
Rodwell 10.76
Grimsley 10.701
Solomon 10.774
Lehninger 10.745
Nozaki 10.555
DTASelect 10.379
Thurlkill 10.54
EMBOSS 10.95
Sillero 10.584
Patrickios 10.496
IPC_peptide 10.774
IPC2_peptide 9.604
IPC2.peptide.svr19 8.595
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4446
0
4446
1402276
29
3856
315.4
34.82
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.73 ± 0.041
1.006 ± 0.012
5.362 ± 0.033
5.542 ± 0.036
3.842 ± 0.025
7.497 ± 0.037
2.281 ± 0.017
5.613 ± 0.03
4.22 ± 0.031
10.772 ± 0.047
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.836 ± 0.019
3.816 ± 0.03
4.432 ± 0.026
4.529 ± 0.03
5.491 ± 0.037
6.074 ± 0.033
5.535 ± 0.038
7.174 ± 0.03
1.526 ± 0.018
2.724 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here