Amino acid dipepetide frequency for Scandinavium goeteborgense

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	10.057AlaAla: 10.057 ± 0.123	1.019AlaCys: 1.019 ± 0.028	5.17AlaAsp: 5.17 ± 0.073	5.748AlaGlu: 5.748 ± 0.076	3.641AlaPhe: 3.641 ± 0.063	7.487AlaGly: 7.487 ± 0.094	1.873AlaHis: 1.873 ± 0.038	5.629AlaIle: 5.629 ± 0.075	4.003AlaLys: 4.003 ± 0.063	11.829AlaLeu: 11.829 ± 0.103	3.053AlaMet: 3.053 ± 0.053	3.233AlaAsn: 3.233 ± 0.056	3.678AlaPro: 3.678 ± 0.06	4.563AlaGln: 4.563 ± 0.068	5.271AlaArg: 5.271 ± 0.077	5.619AlaSer: 5.619 ± 0.065	4.96AlaThr: 4.96 ± 0.072	6.81AlaVal: 6.81 ± 0.072	1.657AlaTrp: 1.657 ± 0.037	1.997AlaTyr: 1.997 ± 0.043	0.001AlaXaa: 0.001 ± 0.001
Cys	0.869CysAla: 0.869 ± 0.028	0.161CysCys: 0.161 ± 0.011	0.594CysAsp: 0.594 ± 0.021	0.592CysGlu: 0.592 ± 0.022	0.411CysPhe: 0.411 ± 0.015	0.993CysGly: 0.993 ± 0.03	0.297CysHis: 0.297 ± 0.015	0.518CysIle: 0.518 ± 0.021	0.312CysLys: 0.312 ± 0.015	1.007CysLeu: 1.007 ± 0.026	0.222CysMet: 0.222 ± 0.012	0.305CysAsn: 0.305 ± 0.016	0.466CysPro: 0.466 ± 0.018	0.436CysGln: 0.436 ± 0.018	0.578CysArg: 0.578 ± 0.022	0.649CysSer: 0.649 ± 0.023	0.47CysThr: 0.47 ± 0.019	0.718CysVal: 0.718 ± 0.023	0.172CysTrp: 0.172 ± 0.012	0.287CysTyr: 0.287 ± 0.013	0.0CysXaa: 0.0 ± 0.0
Asp	5.582AspAla: 5.582 ± 0.067	0.484AspCys: 0.484 ± 0.022	3.267AspAsp: 3.267 ± 0.064	3.5AspGlu: 3.5 ± 0.058	2.105AspPhe: 2.105 ± 0.049	4.126AspGly: 4.126 ± 0.071	1.003AspHis: 1.003 ± 0.025	3.439AspIle: 3.439 ± 0.05	2.593AspLys: 2.593 ± 0.052	4.644AspLeu: 4.644 ± 0.059	1.38AspMet: 1.38 ± 0.035	2.379AspAsn: 2.379 ± 0.056	2.293AspPro: 2.293 ± 0.044	1.672AspGln: 1.672 ± 0.038	2.634AspArg: 2.634 ± 0.046	3.018AspSer: 3.018 ± 0.051	2.854AspThr: 2.854 ± 0.049	4.046AspVal: 4.046 ± 0.061	0.821AspTrp: 0.821 ± 0.026	1.776AspTyr: 1.776 ± 0.042	0.0AspXaa: 0.0 ± 0.0
Glu	5.104GluAla: 5.104 ± 0.084	0.471GluCys: 0.471 ± 0.017	2.44GluAsp: 2.44 ± 0.049	3.194GluGlu: 3.194 ± 0.073	1.862GluPhe: 1.862 ± 0.043	3.697GluGly: 3.697 ± 0.055	1.39GluHis: 1.39 ± 0.033	3.213GluIle: 3.213 ± 0.048	3.168GluLys: 3.168 ± 0.053	5.853GluLeu: 5.853 ± 0.082	1.861GluMet: 1.861 ± 0.038	2.324GluAsn: 2.324 ± 0.044	2.1GluPro: 2.1 ± 0.045	3.308GluGln: 3.308 ± 0.062	3.521GluArg: 3.521 ± 0.058	3.042GluSer: 3.042 ± 0.052	2.951GluThr: 2.951 ± 0.049	3.698GluVal: 3.698 ± 0.055	0.792GluTrp: 0.792 ± 0.025	1.428GluTyr: 1.428 ± 0.03	0.0GluXaa: 0.0 ± 0.0
Phe	3.765PheAla: 3.765 ± 0.057	0.513PheCys: 0.513 ± 0.019	2.341PheAsp: 2.341 ± 0.044	1.763PheGlu: 1.763 ± 0.035	1.649PhePhe: 1.649 ± 0.039	3.25PheGly: 3.25 ± 0.054	0.769PheHis: 0.769 ± 0.025	2.484PheIle: 2.484 ± 0.051	1.281PheLys: 1.281 ± 0.032	3.245PheLeu: 3.245 ± 0.056	1.024PheMet: 1.024 ± 0.028	1.757PheAsn: 1.757 ± 0.036	1.535PhePro: 1.535 ± 0.034	1.161PheGln: 1.161 ± 0.029	1.87PheArg: 1.87 ± 0.038	3.145PheSer: 3.145 ± 0.051	2.517PheThr: 2.517 ± 0.057	2.571PheVal: 2.571 ± 0.053	0.635PheTrp: 0.635 ± 0.025	1.149PheTyr: 1.149 ± 0.028	0.0PheXaa: 0.0 ± 0.0
Gly	6.281GlyAla: 6.281 ± 0.088	0.961GlyCys: 0.961 ± 0.027	3.919GlyAsp: 3.919 ± 0.058	4.538GlyGlu: 4.538 ± 0.068	3.258GlyPhe: 3.258 ± 0.053	5.553GlyGly: 5.553 ± 0.085	1.691GlyHis: 1.691 ± 0.037	4.874GlyIle: 4.874 ± 0.065	4.038GlyLys: 4.038 ± 0.064	7.611GlyLeu: 7.611 ± 0.093	2.467GlyMet: 2.467 ± 0.05	2.893GlyAsn: 2.893 ± 0.082	2.095GlyPro: 2.095 ± 0.04	3.021GlyGln: 3.021 ± 0.051	3.648GlyArg: 3.648 ± 0.059	4.407GlySer: 4.407 ± 0.079	3.961GlyThr: 3.961 ± 0.08	5.83GlyVal: 5.83 ± 0.072	1.311GlyTrp: 1.311 ± 0.033	2.614GlyTyr: 2.614 ± 0.051	0.0GlyXaa: 0.0 ± 0.0
His	2.029HisAla: 2.029 ± 0.039	0.29HisCys: 0.29 ± 0.015	1.252HisAsp: 1.252 ± 0.029	1.082HisGlu: 1.082 ± 0.028	1.072HisPhe: 1.072 ± 0.027	1.791HisGly: 1.791 ± 0.04	0.757HisHis: 0.757 ± 0.028	1.286HisIle: 1.286 ± 0.032	0.754HisLys: 0.754 ± 0.025	2.264HisLeu: 2.264 ± 0.044	0.551HisMet: 0.551 ± 0.019	0.859HisAsn: 0.859 ± 0.023	1.284HisPro: 1.284 ± 0.033	1.165HisGln: 1.165 ± 0.032	1.22HisArg: 1.22 ± 0.032	1.313HisSer: 1.313 ± 0.034	1.136HisThr: 1.136 ± 0.027	1.394HisVal: 1.394 ± 0.033	0.42HisTrp: 0.42 ± 0.018	0.893HisTyr: 0.893 ± 0.024	0.0HisXaa: 0.0 ± 0.0
Ile	6.218IleAla: 6.218 ± 0.068	0.627IleCys: 0.627 ± 0.023	3.516IleAsp: 3.516 ± 0.058	3.188IleGlu: 3.188 ± 0.056	2.042IlePhe: 2.042 ± 0.041	4.496IleGly: 4.496 ± 0.068	1.163IleHis: 1.163 ± 0.032	3.183IleIle: 3.183 ± 0.067	2.239IleLys: 2.239 ± 0.04	4.88IleLeu: 4.88 ± 0.062	1.244IleMet: 1.244 ± 0.031	2.593IleAsn: 2.593 ± 0.05	2.606IlePro: 2.606 ± 0.04	1.823IleGln: 1.823 ± 0.041	2.845IleArg: 2.845 ± 0.05	3.847IleSer: 3.847 ± 0.059	3.532IleThr: 3.532 ± 0.059	3.934IleVal: 3.934 ± 0.057	0.673IleTrp: 0.673 ± 0.024	1.476IleTyr: 1.476 ± 0.031	0.0IleXaa: 0.0 ± 0.0
Lys	4.276LysAla: 4.276 ± 0.068	0.27LysCys: 0.27 ± 0.014	2.178LysAsp: 2.178 ± 0.047	2.447LysGlu: 2.447 ± 0.049	1.157LysPhe: 1.157 ± 0.031	3.002LysGly: 3.002 ± 0.053	0.876LysHis: 0.876 ± 0.028	2.313LysIle: 2.313 ± 0.049	2.199LysLys: 2.199 ± 0.055	4.305LysLeu: 4.305 ± 0.058	1.287LysMet: 1.287 ± 0.031	1.677LysAsn: 1.677 ± 0.044	2.191LysPro: 2.191 ± 0.045	1.989LysGln: 1.989 ± 0.041	2.507LysArg: 2.507 ± 0.051	2.365LysSer: 2.365 ± 0.046	2.616LysThr: 2.616 ± 0.046	2.967LysVal: 2.967 ± 0.056	0.461LysTrp: 0.461 ± 0.018	1.113LysTyr: 1.113 ± 0.033	0.0LysXaa: 0.0 ± 0.0
Leu	11.3LeuAla: 11.3 ± 0.11	1.244LeuCys: 1.244 ± 0.028	5.485LeuAsp: 5.485 ± 0.076	5.39LeuGlu: 5.39 ± 0.082	4.213LeuPhe: 4.213 ± 0.067	7.277LeuGly: 7.277 ± 0.077	2.348LeuHis: 2.348 ± 0.045	5.652LeuIle: 5.652 ± 0.072	4.583LeuLys: 4.583 ± 0.062	11.732LeuLeu: 11.732 ± 0.135	3.051LeuMet: 3.051 ± 0.047	4.432LeuAsn: 4.432 ± 0.072	5.56LeuPro: 5.56 ± 0.067	4.144LeuGln: 4.144 ± 0.059	5.987LeuArg: 5.987 ± 0.077	7.531LeuSer: 7.531 ± 0.089	6.527LeuThr: 6.527 ± 0.075	7.256LeuVal: 7.256 ± 0.081	1.5LeuTrp: 1.5 ± 0.038	2.507LeuTyr: 2.507 ± 0.042	0.0LeuXaa: 0.0 ± 0.0
Met	2.96MetAla: 2.96 ± 0.046	0.193MetCys: 0.193 ± 0.011	1.269MetAsp: 1.269 ± 0.027	1.231MetGlu: 1.231 ± 0.032	0.953MetPhe: 0.953 ± 0.029	1.942MetGly: 1.942 ± 0.038	0.552MetHis: 0.552 ± 0.021	1.464MetIle: 1.464 ± 0.033	1.498MetLys: 1.498 ± 0.033	3.253MetLeu: 3.253 ± 0.061	0.978MetMet: 0.978 ± 0.027	1.15MetAsn: 1.15 ± 0.027	1.421MetPro: 1.421 ± 0.028	1.294MetGln: 1.294 ± 0.026	1.527MetArg: 1.527 ± 0.037	1.971MetSer: 1.971 ± 0.037	1.881MetThr: 1.881 ± 0.041	2.004MetVal: 2.004 ± 0.038	0.261MetTrp: 0.261 ± 0.014	0.553MetTyr: 0.553 ± 0.021	0.0MetXaa: 0.0 ± 0.0
Asn	3.695AsnAla: 3.695 ± 0.058	0.343AsnCys: 0.343 ± 0.017	2.168AsnAsp: 2.168 ± 0.045	1.955AsnGlu: 1.955 ± 0.042	1.342AsnPhe: 1.342 ± 0.032	3.24AsnGly: 3.24 ± 0.083	0.851AsnHis: 0.851 ± 0.025	2.297AsnIle: 2.297 ± 0.042	1.555AsnLys: 1.555 ± 0.035	3.561AsnLeu: 3.561 ± 0.053	0.991AsnMet: 0.991 ± 0.029	1.626AsnAsn: 1.626 ± 0.047	2.104AsnPro: 2.104 ± 0.035	1.647AsnGln: 1.647 ± 0.034	2.013AsnArg: 2.013 ± 0.037	2.161AsnSer: 2.161 ± 0.049	2.107AsnThr: 2.107 ± 0.046	2.708AsnVal: 2.708 ± 0.054	0.61AsnTrp: 0.61 ± 0.022	1.186AsnTyr: 1.186 ± 0.034	0.0AsnXaa: 0.0 ± 0.0
Pro	4.402ProAla: 4.402 ± 0.062	0.358ProCys: 0.358 ± 0.015	2.849ProAsp: 2.849 ± 0.051	3.288ProGlu: 3.288 ± 0.054	1.806ProPhe: 1.806 ± 0.037	3.486ProGly: 3.486 ± 0.055	1.035ProHis: 1.035 ± 0.029	1.843ProIle: 1.843 ± 0.041	1.549ProLys: 1.549 ± 0.034	5.01ProLeu: 5.01 ± 0.065	1.179ProMet: 1.179 ± 0.029	1.314ProAsn: 1.314 ± 0.03	1.612ProPro: 1.612 ± 0.04	2.317ProGln: 2.317 ± 0.048	1.918ProArg: 1.918 ± 0.05	2.195ProSer: 2.195 ± 0.041	2.291ProThr: 2.291 ± 0.052	3.995ProVal: 3.995 ± 0.058	0.768ProTrp: 0.768 ± 0.025	1.1ProTyr: 1.1 ± 0.03	0.0ProXaa: 0.0 ± 0.0
Gln	4.682GlnAla: 4.682 ± 0.07	0.363GlnCys: 0.363 ± 0.016	1.979GlnAsp: 1.979 ± 0.039	2.159GlnGlu: 2.159 ± 0.05	1.508GlnPhe: 1.508 ± 0.034	3.149GlnGly: 3.149 ± 0.052	1.242GlnHis: 1.242 ± 0.03	2.224GlnIle: 2.224 ± 0.036	1.89GlnLys: 1.89 ± 0.041	5.029GlnLeu: 5.029 ± 0.063	1.261GlnMet: 1.261 ± 0.03	1.506GlnAsn: 1.506 ± 0.032	2.229GlnPro: 2.229 ± 0.048	3.428GlnGln: 3.428 ± 0.082	2.934GlnArg: 2.934 ± 0.053	2.476GlnSer: 2.476 ± 0.044	2.341GlnThr: 2.341 ± 0.044	3.023GlnVal: 3.023 ± 0.045	0.7GlnTrp: 0.7 ± 0.022	1.167GlnTyr: 1.167 ± 0.026	0.0GlnXaa: 0.0 ± 0.0
Arg	4.356ArgAla: 4.356 ± 0.062	0.533ArgCys: 0.533 ± 0.022	2.994ArgAsp: 2.994 ± 0.053	3.736ArgGlu: 3.736 ± 0.059	2.49ArgPhe: 2.49 ± 0.046	3.288ArgGly: 3.288 ± 0.051	1.568ArgHis: 1.568 ± 0.036	3.202ArgIle: 3.202 ± 0.05	2.342ArgLys: 2.342 ± 0.046	6.204ArgLeu: 6.204 ± 0.073	1.612ArgMet: 1.612 ± 0.028	2.035ArgAsn: 2.035 ± 0.044	2.108ArgPro: 2.108 ± 0.041	2.95ArgGln: 2.95 ± 0.053	3.33ArgArg: 3.33 ± 0.06	2.795ArgSer: 2.795 ± 0.047	2.482ArgThr: 2.482 ± 0.04	3.827ArgVal: 3.827 ± 0.053	0.972ArgTrp: 0.972 ± 0.028	2.089ArgTyr: 2.089 ± 0.042	0.0ArgXaa: 0.0 ± 0.0
Ser	5.868SerAla: 5.868 ± 0.089	0.501SerCys: 0.501 ± 0.02	3.229SerAsp: 3.229 ± 0.047	3.172SerGlu: 3.172 ± 0.05	2.356SerPhe: 2.356 ± 0.043	5.463SerGly: 5.463 ± 0.09	1.544SerHis: 1.544 ± 0.034	3.032SerIle: 3.032 ± 0.043	2.072SerLys: 2.072 ± 0.036	6.911SerLeu: 6.911 ± 0.092	1.615SerMet: 1.615 ± 0.036	2.03SerAsn: 2.03 ± 0.041	2.695SerPro: 2.695 ± 0.043	2.762SerGln: 2.762 ± 0.052	3.487SerArg: 3.487 ± 0.059	3.763SerSer: 3.763 ± 0.072	3.172SerThr: 3.172 ± 0.063	4.443SerVal: 4.443 ± 0.066	0.986SerTrp: 0.986 ± 0.029	1.64SerTyr: 1.64 ± 0.04	0.0SerXaa: 0.0 ± 0.0
Thr	5.294ThrAla: 5.294 ± 0.094	0.477ThrCys: 0.477 ± 0.018	2.887ThrAsp: 2.887 ± 0.055	2.768ThrGlu: 2.768 ± 0.051	2.129ThrPhe: 2.129 ± 0.034	4.645ThrGly: 4.645 ± 0.086	1.297ThrHis: 1.297 ± 0.034	2.826ThrIle: 2.826 ± 0.05	1.535ThrLys: 1.535 ± 0.04	7.746ThrLeu: 7.746 ± 0.101	1.201ThrMet: 1.201 ± 0.026	1.629ThrAsn: 1.629 ± 0.045	3.24ThrPro: 3.24 ± 0.062	2.342ThrGln: 2.342 ± 0.039	3.083ThrArg: 3.083 ± 0.048	3.034ThrSer: 3.034 ± 0.055	3.069ThrThr: 3.069 ± 0.067	4.064ThrVal: 4.064 ± 0.075	0.866ThrTrp: 0.866 ± 0.027	1.217ThrTyr: 1.217 ± 0.034	0.0ThrXaa: 0.0 ± 0.0
Val	7.146ValAla: 7.146 ± 0.075	0.745ValCys: 0.745 ± 0.024	3.847ValAsp: 3.847 ± 0.05	3.864ValGlu: 3.864 ± 0.067	2.622ValPhe: 2.622 ± 0.047	4.95ValGly: 4.95 ± 0.073	1.336ValHis: 1.336 ± 0.032	4.509ValIle: 4.509 ± 0.057	3.125ValLys: 3.125 ± 0.051	7.417ValLeu: 7.417 ± 0.083	2.328ValMet: 2.328 ± 0.042	2.918ValAsn: 2.918 ± 0.05	3.148ValPro: 3.148 ± 0.048	2.513ValGln: 2.513 ± 0.045	3.638ValArg: 3.638 ± 0.051	4.802ValSer: 4.802 ± 0.06	4.342ValThr: 4.342 ± 0.064	5.794ValVal: 5.794 ± 0.074	0.996ValTrp: 0.996 ± 0.023	1.704ValTyr: 1.704 ± 0.036	0.0ValXaa: 0.0 ± 0.0
Trp	1.017TrpAla: 1.017 ± 0.029	0.175TrpCys: 0.175 ± 0.012	0.667TrpAsp: 0.667 ± 0.024	0.618TrpGlu: 0.618 ± 0.022	0.693TrpPhe: 0.693 ± 0.024	0.932TrpGly: 0.932 ± 0.03	0.486TrpHis: 0.486 ± 0.019	0.732TrpIle: 0.732 ± 0.024	0.548TrpLys: 0.548 ± 0.017	2.352TrpLeu: 2.352 ± 0.052	0.461TrpMet: 0.461 ± 0.019	0.508TrpAsn: 0.508 ± 0.021	0.656TrpPro: 0.656 ± 0.022	1.207TrpGln: 1.207 ± 0.035	1.095TrpArg: 1.095 ± 0.032	0.852TrpSer: 0.852 ± 0.025	0.621TrpThr: 0.621 ± 0.022	0.979TrpVal: 0.979 ± 0.027	0.222TrpTrp: 0.222 ± 0.012	0.435TrpTyr: 0.435 ± 0.019	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.398TyrAla: 2.398 ± 0.045	0.329TyrCys: 0.329 ± 0.017	1.566TyrAsp: 1.566 ± 0.039	1.185TyrGlu: 1.185 ± 0.034	1.114TyrPhe: 1.114 ± 0.032	2.203TyrGly: 2.203 ± 0.051	0.734TyrHis: 0.734 ± 0.024	1.406TyrIle: 1.406 ± 0.033	0.917TyrLys: 0.917 ± 0.029	2.866TyrLeu: 2.866 ± 0.044	0.588TyrMet: 0.588 ± 0.022	0.991TyrAsn: 0.991 ± 0.029	1.292TyrPro: 1.292 ± 0.039	1.546TyrGln: 1.546 ± 0.036	1.806TyrArg: 1.806 ± 0.037	1.754TyrSer: 1.754 ± 0.042	1.52TyrThr: 1.52 ± 0.034	1.684TyrVal: 1.684 ± 0.032	0.433TyrTrp: 0.433 ± 0.017	0.905TyrTyr: 0.905 ± 0.027	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.001XaaSer: 0.001 ± 0.001	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4446 proteins (1402276 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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