Staphylococcus epidermidis (strain ATCC 35984 / RP62A)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Staphylococcaceae; Staphylococcus; Staphylococcus epidermidis

Average proteome isoelectric point is 6.58

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2492 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q5HMW7|Q5HMW7_STAEQ Uncharacterized protein OS=Staphylococcus epidermidis (strain ATCC 35984 / RP62A) OX=176279 GN=SERP1509 PE=4 SV=1
MM1 pKa = 7.26NALEE5 pKa = 4.12AAYY8 pKa = 9.99KK9 pKa = 10.61NVTFDD14 pKa = 3.69YY15 pKa = 11.46LEE17 pKa = 5.7DD18 pKa = 3.83NDD20 pKa = 5.18TYY22 pKa = 12.0DD23 pKa = 3.16NTQDD27 pKa = 3.25FNEE30 pKa = 5.24AIHH33 pKa = 5.24TTEE36 pKa = 5.58DD37 pKa = 3.26FLEE40 pKa = 4.22NLDD43 pKa = 3.53YY44 pKa = 11.6NFYY47 pKa = 10.94DD48 pKa = 3.89KK49 pKa = 11.06KK50 pKa = 11.13

Molecular weight: 6.02 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q5HS60|Q5HS60_STAEQ Lactose phosphotransferase system repressor OS=Staphylococcus epidermidis (strain ATCC 35984 / RP62A) OX=176279 GN=SEA0009 PE=4 SV=1
MM1 pKa = 7.49VKK3 pKa = 9.1RR4 pKa = 11.84TYY6 pKa = 10.35QPNKK10 pKa = 8.16RR11 pKa = 11.84KK12 pKa = 9.54HH13 pKa = 5.99SKK15 pKa = 8.87VHH17 pKa = 5.77GFRR20 pKa = 11.84KK21 pKa = 10.02RR22 pKa = 11.84MSTKK26 pKa = 10.18NGRR29 pKa = 11.84KK30 pKa = 8.49VLARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84KK38 pKa = 9.05GRR40 pKa = 11.84KK41 pKa = 8.7VLSAA45 pKa = 4.05

Molecular weight: 5.43 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2492

0

2492

714899

19

9439

286.9

32.47

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.898 ± 0.058

0.633 ± 0.017

5.719 ± 0.052

6.775 ± 0.066

4.442 ± 0.063

6.025 ± 0.061

2.449 ± 0.031

8.805 ± 0.076

7.656 ± 0.061

9.137 ± 0.073

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.601 ± 0.033

5.869 ± 0.084

3.137 ± 0.045

4.202 ± 0.077

3.579 ± 0.036

6.267 ± 0.079

5.701 ± 0.071

6.468 ± 0.041

0.715 ± 0.02

3.922 ± 0.045
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski