Amino acid dipepetide frequency for Staphylococcus epidermidis (strain ATCC 35984 / RP62A)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
3.531AlaAla: 3.531 ± 0.099
0.474AlaCys: 0.474 ± 0.027
2.658AlaAsp: 2.658 ± 0.072
3.242AlaGlu: 3.242 ± 0.082
2.777AlaPhe: 2.777 ± 0.072
3.739AlaGly: 3.739 ± 0.097
1.375AlaHis: 1.375 ± 0.044
5.939AlaIle: 5.939 ± 0.125
4.515AlaLys: 4.515 ± 0.158
6.142AlaLeu: 6.142 ± 0.134
1.637AlaMet: 1.637 ± 0.05
2.974AlaAsn: 2.974 ± 0.093
1.681AlaPro: 1.681 ± 0.061
2.64AlaGln: 2.64 ± 0.115
2.142AlaArg: 2.142 ± 0.059
3.738AlaSer: 3.738 ± 0.106
3.535AlaThr: 3.535 ± 0.12
3.749AlaVal: 3.749 ± 0.091
0.347AlaTrp: 0.347 ± 0.025
2.15AlaTyr: 2.15 ± 0.056
0.0AlaXaa: 0.0 ± 0.0
Cys
0.347CysAla: 0.347 ± 0.025
0.083CysCys: 0.083 ± 0.011
0.362CysAsp: 0.362 ± 0.027
0.355CysGlu: 0.355 ± 0.026
0.245CysPhe: 0.245 ± 0.021
0.575CysGly: 0.575 ± 0.03
0.222CysHis: 0.222 ± 0.02
0.55CysIle: 0.55 ± 0.028
0.387CysLys: 0.387 ± 0.026
0.594CysLeu: 0.594 ± 0.03
0.145CysMet: 0.145 ± 0.013
0.287CysAsn: 0.287 ± 0.023
0.28CysPro: 0.28 ± 0.023
0.187CysGln: 0.187 ± 0.017
0.21CysArg: 0.21 ± 0.016
0.389CysSer: 0.389 ± 0.027
0.421CysThr: 0.421 ± 0.026
0.364CysVal: 0.364 ± 0.021
0.046CysTrp: 0.046 ± 0.008
0.278CysTyr: 0.278 ± 0.02
0.0CysXaa: 0.0 ± 0.0
Asp
3.31AspAla: 3.31 ± 0.103
0.344AspCys: 0.344 ± 0.026
3.36AspAsp: 3.36 ± 0.088
4.769AspGlu: 4.769 ± 0.098
2.575AspPhe: 2.575 ± 0.071
3.16AspGly: 3.16 ± 0.073
1.213AspHis: 1.213 ± 0.041
5.546AspIle: 5.546 ± 0.1
4.666AspLys: 4.666 ± 0.101
4.922AspLeu: 4.922 ± 0.084
1.453AspMet: 1.453 ± 0.048
3.192AspAsn: 3.192 ± 0.089
1.388AspPro: 1.388 ± 0.063
2.087AspGln: 2.087 ± 0.074
1.831AspArg: 1.831 ± 0.058
3.226AspSer: 3.226 ± 0.14
3.026AspThr: 3.026 ± 0.067
3.936AspVal: 3.936 ± 0.09
0.457AspTrp: 0.457 ± 0.028
2.733AspTyr: 2.733 ± 0.084
0.0AspXaa: 0.0 ± 0.0
Glu
4.408GluAla: 4.408 ± 0.136
0.302GluCys: 0.302 ± 0.022
4.103GluAsp: 4.103 ± 0.081
5.264GluGlu: 5.264 ± 0.121
2.465GluPhe: 2.465 ± 0.075
3.566GluGly: 3.566 ± 0.09
1.621GluHis: 1.621 ± 0.052
5.51GluIle: 5.51 ± 0.109
5.497GluLys: 5.497 ± 0.106
6.11GluLeu: 6.11 ± 0.101
1.996GluMet: 1.996 ± 0.057
4.098GluAsn: 4.098 ± 0.091
1.732GluPro: 1.732 ± 0.08
3.188GluGln: 3.188 ± 0.095
3.019GluArg: 3.019 ± 0.074
3.799GluSer: 3.799 ± 0.109
3.718GluThr: 3.718 ± 0.079
4.483GluVal: 4.483 ± 0.075
0.543GluTrp: 0.543 ± 0.027
2.328GluTyr: 2.328 ± 0.061
0.0GluXaa: 0.0 ± 0.0
Phe
2.347PheAla: 2.347 ± 0.075
0.326PheCys: 0.326 ± 0.022
2.77PheAsp: 2.77 ± 0.09
2.984PheGlu: 2.984 ± 0.081
1.999PhePhe: 1.999 ± 0.074
2.928PheGly: 2.928 ± 0.089
0.839PheHis: 0.839 ± 0.034
4.623PheIle: 4.623 ± 0.109
3.733PheLys: 3.733 ± 0.085
3.712PheLeu: 3.712 ± 0.11
1.064PheMet: 1.064 ± 0.042
3.065PheAsn: 3.065 ± 0.077
1.22PhePro: 1.22 ± 0.044
1.23PheGln: 1.23 ± 0.039
1.19PheArg: 1.19 ± 0.04
2.894PheSer: 2.894 ± 0.062
2.523PheThr: 2.523 ± 0.061
2.947PheVal: 2.947 ± 0.084
0.347PheTrp: 0.347 ± 0.022
1.677PheTyr: 1.677 ± 0.058
0.0PheXaa: 0.0 ± 0.0
Gly
4.015GlyAla: 4.015 ± 0.115
0.471GlyCys: 0.471 ± 0.025
3.034GlyAsp: 3.034 ± 0.09
3.518GlyGlu: 3.518 ± 0.126
3.01GlyPhe: 3.01 ± 0.092
4.093GlyGly: 4.093 ± 0.125
1.56GlyHis: 1.56 ± 0.054
5.483GlyIle: 5.483 ± 0.126
4.538GlyLys: 4.538 ± 0.098
5.566GlyLeu: 5.566 ± 0.12
1.698GlyMet: 1.698 ± 0.054
2.652GlyAsn: 2.652 ± 0.066
1.504GlyPro: 1.504 ± 0.052
2.343GlyGln: 2.343 ± 0.066
2.199GlyArg: 2.199 ± 0.066
3.526GlySer: 3.526 ± 0.084
3.497GlyThr: 3.497 ± 0.087
4.324GlyVal: 4.324 ± 0.09
0.518GlyTrp: 0.518 ± 0.029
2.702GlyTyr: 2.702 ± 0.061
0.0GlyXaa: 0.0 ± 0.0
His
1.389HisAla: 1.389 ± 0.047
0.186HisCys: 0.186 ± 0.018
1.288HisAsp: 1.288 ± 0.044
1.33HisGlu: 1.33 ± 0.044
1.287HisPhe: 1.287 ± 0.039
1.413HisGly: 1.413 ± 0.054
0.902HisHis: 0.902 ± 0.037
2.112HisIle: 2.112 ± 0.058
1.445HisLys: 1.445 ± 0.055
2.479HisLeu: 2.479 ± 0.071
0.62HisMet: 0.62 ± 0.028
1.141HisAsn: 1.141 ± 0.045
1.044HisPro: 1.044 ± 0.04
1.297HisGln: 1.297 ± 0.049
0.932HisArg: 0.932 ± 0.035
1.48HisSer: 1.48 ± 0.043
1.343HisThr: 1.343 ± 0.048
1.548HisVal: 1.548 ± 0.049
0.178HisTrp: 0.178 ± 0.016
1.078HisTyr: 1.078 ± 0.041
0.0HisXaa: 0.0 ± 0.0
Ile
5.819IleAla: 5.819 ± 0.104
0.628IleCys: 0.628 ± 0.036
5.42IleAsp: 5.42 ± 0.088
6.152IleGlu: 6.152 ± 0.113
3.789IlePhe: 3.789 ± 0.107
5.791IleGly: 5.791 ± 0.127
1.946IleHis: 1.946 ± 0.057
7.798IleIle: 7.798 ± 0.156
6.608IleLys: 6.608 ± 0.106
7.523IleLeu: 7.523 ± 0.149
2.021IleMet: 2.021 ± 0.068
5.398IleAsn: 5.398 ± 0.217
3.115IlePro: 3.115 ± 0.069
3.371IleGln: 3.371 ± 0.086
2.666IleArg: 2.666 ± 0.062
5.706IleSer: 5.706 ± 0.107
4.787IleThr: 4.787 ± 0.085
6.068IleVal: 6.068 ± 0.126
0.495IleTrp: 0.495 ± 0.027
2.95IleTyr: 2.95 ± 0.069
0.0IleXaa: 0.0 ± 0.0
Lys
4.454LysAla: 4.454 ± 0.106
0.355LysCys: 0.355 ± 0.023
5.31LysAsp: 5.31 ± 0.105
6.486LysGlu: 6.486 ± 0.12
2.452LysPhe: 2.452 ± 0.067
4.357LysGly: 4.357 ± 0.093
1.842LysHis: 1.842 ± 0.058
5.353LysIle: 5.353 ± 0.099
6.243LysLys: 6.243 ± 0.128
6.201LysLeu: 6.201 ± 0.104
2.251LysMet: 2.251 ± 0.062
4.844LysAsn: 4.844 ± 0.11
2.323LysPro: 2.323 ± 0.075
3.896LysGln: 3.896 ± 0.119
3.216LysArg: 3.216 ± 0.065
4.415LysSer: 4.415 ± 0.085
3.886LysThr: 3.886 ± 0.087
5.045LysVal: 5.045 ± 0.094
0.567LysTrp: 0.567 ± 0.031
3.062LysTyr: 3.062 ± 0.087
0.0LysXaa: 0.0 ± 0.0
Leu
5.458LeuAla: 5.458 ± 0.096
0.627LeuCys: 0.627 ± 0.031
4.844LeuAsp: 4.844 ± 0.096
5.672LeuGlu: 5.672 ± 0.111
4.329LeuPhe: 4.329 ± 0.126
5.559LeuGly: 5.559 ± 0.141
1.758LeuHis: 1.758 ± 0.056
8.299LeuIle: 8.299 ± 0.154
7.239LeuLys: 7.239 ± 0.117
8.57LeuLeu: 8.57 ± 0.172
2.347LeuMet: 2.347 ± 0.067
6.271LeuAsn: 6.271 ± 0.182
3.269LeuPro: 3.269 ± 0.065
2.978LeuGln: 2.978 ± 0.103
3.031LeuArg: 3.031 ± 0.081
6.668LeuSer: 6.668 ± 0.112
5.478LeuThr: 5.478 ± 0.088
5.32LeuVal: 5.32 ± 0.101
0.554LeuTrp: 0.554 ± 0.033
3.1LeuTyr: 3.1 ± 0.082
0.0LeuXaa: 0.0 ± 0.0
Met
1.592MetAla: 1.592 ± 0.06
0.157MetCys: 0.157 ± 0.015
1.224MetAsp: 1.224 ± 0.043
1.383MetGlu: 1.383 ± 0.042
1.095MetPhe: 1.095 ± 0.048
1.425MetGly: 1.425 ± 0.055
0.519MetHis: 0.519 ± 0.026
2.551MetIle: 2.551 ± 0.069
2.363MetLys: 2.363 ± 0.053
2.241MetLeu: 2.241 ± 0.062
0.827MetMet: 0.827 ± 0.037
1.863MetAsn: 1.863 ± 0.045
0.857MetPro: 0.857 ± 0.04
0.821MetGln: 0.821 ± 0.036
0.944MetArg: 0.944 ± 0.039
1.963MetSer: 1.963 ± 0.06
1.776MetThr: 1.776 ± 0.058
1.444MetVal: 1.444 ± 0.054
0.144MetTrp: 0.144 ± 0.014
0.821MetTyr: 0.821 ± 0.032
0.0MetXaa: 0.0 ± 0.0
Asn
3.273AsnAla: 3.273 ± 0.175
0.319AsnCys: 0.319 ± 0.023
3.691AsnAsp: 3.691 ± 0.107
4.61AsnGlu: 4.61 ± 0.132
2.142AsnPhe: 2.142 ± 0.057
3.283AsnGly: 3.283 ± 0.092
1.937AsnHis: 1.937 ± 0.054
4.904AsnIle: 4.904 ± 0.096
4.962AsnLys: 4.962 ± 0.115
4.45AsnLeu: 4.45 ± 0.093
1.39AsnMet: 1.39 ± 0.046
3.58AsnAsn: 3.58 ± 0.164
2.042AsnPro: 2.042 ± 0.067
3.312AsnGln: 3.312 ± 0.139
2.139AsnArg: 2.139 ± 0.05
3.068AsnSer: 3.068 ± 0.123
2.929AsnThr: 2.929 ± 0.105
3.649AsnVal: 3.649 ± 0.066
0.432AsnTrp: 0.432 ± 0.026
2.577AsnTyr: 2.577 ± 0.082
0.0AsnXaa: 0.0 ± 0.0
Pro
1.431ProAla: 1.431 ± 0.055
0.162ProCys: 0.162 ± 0.015
1.606ProAsp: 1.606 ± 0.05
2.332ProGlu: 2.332 ± 0.068
1.688ProPhe: 1.688 ± 0.056
1.904ProGly: 1.904 ± 0.113
0.871ProHis: 0.871 ± 0.041
2.774ProIle: 2.774 ± 0.064
2.223ProLys: 2.223 ± 0.059
2.949ProLeu: 2.949 ± 0.064
0.716ProMet: 0.716 ± 0.032
1.902ProAsn: 1.902 ± 0.067
0.701ProPro: 0.701 ± 0.036
1.253ProGln: 1.253 ± 0.042
0.944ProArg: 0.944 ± 0.04
2.084ProSer: 2.084 ± 0.054
2.017ProThr: 2.017 ± 0.079
2.234ProVal: 2.234 ± 0.086
0.236ProTrp: 0.236 ± 0.021
1.343ProTyr: 1.343 ± 0.046
0.0ProXaa: 0.0 ± 0.0
Gln
2.627GlnAla: 2.627 ± 0.167
0.207GlnCys: 0.207 ± 0.017
2.041GlnAsp: 2.041 ± 0.059
2.335GlnGlu: 2.335 ± 0.067
2.116GlnPhe: 2.116 ± 0.062
1.89GlnGly: 1.89 ± 0.056
1.192GlnHis: 1.192 ± 0.04
3.139GlnIle: 3.139 ± 0.083
2.711GlnLys: 2.711 ± 0.104
4.528GlnLeu: 4.528 ± 0.109
1.123GlnMet: 1.123 ± 0.041
2.501GlnAsn: 2.501 ± 0.107
1.35GlnPro: 1.35 ± 0.05
2.414GlnGln: 2.414 ± 0.129
1.716GlnArg: 1.716 ± 0.059
3.089GlnSer: 3.089 ± 0.095
2.501GlnThr: 2.501 ± 0.092
2.372GlnVal: 2.372 ± 0.069
0.386GlnTrp: 0.386 ± 0.024
1.779GlnTyr: 1.779 ± 0.054
0.0GlnXaa: 0.0 ± 0.0
Arg
2.024ArgAla: 2.024 ± 0.062
0.204ArgCys: 0.204 ± 0.018
2.066ArgAsp: 2.066 ± 0.058
2.56ArgGlu: 2.56 ± 0.076
1.642ArgPhe: 1.642 ± 0.052
1.956ArgGly: 1.956 ± 0.055
0.971ArgHis: 0.971 ± 0.035
2.826ArgIle: 2.826 ± 0.075
2.799ArgLys: 2.799 ± 0.067
3.489ArgLeu: 3.489 ± 0.091
1.041ArgMet: 1.041 ± 0.048
1.923ArgAsn: 1.923 ± 0.05
1.08ArgPro: 1.08 ± 0.046
1.683ArgGln: 1.683 ± 0.052
1.597ArgArg: 1.597 ± 0.062
1.879ArgSer: 1.879 ± 0.055
1.865ArgThr: 1.865 ± 0.051
2.307ArgVal: 2.307 ± 0.068
0.252ArgTrp: 0.252 ± 0.017
1.63ArgTyr: 1.63 ± 0.055
0.0ArgXaa: 0.0 ± 0.0
Ser
3.293SerAla: 3.293 ± 0.09
0.313SerCys: 0.313 ± 0.02
3.512SerAsp: 3.512 ± 0.127
4.09SerGlu: 4.09 ± 0.102
2.953SerPhe: 2.953 ± 0.072
3.968SerGly: 3.968 ± 0.101
1.628SerHis: 1.628 ± 0.048
5.581SerIle: 5.581 ± 0.114
4.914SerLys: 4.914 ± 0.089
5.818SerLeu: 5.818 ± 0.094
1.648SerMet: 1.648 ± 0.053
3.684SerAsn: 3.684 ± 0.107
1.778SerPro: 1.778 ± 0.055
2.633SerGln: 2.633 ± 0.066
2.205SerArg: 2.205 ± 0.056
4.029SerSer: 4.029 ± 0.111
3.735SerThr: 3.735 ± 0.234
3.872SerVal: 3.872 ± 0.08
0.462SerTrp: 0.462 ± 0.029
2.556SerTyr: 2.556 ± 0.059
0.0SerXaa: 0.0 ± 0.0
Thr
3.172ThrAla: 3.172 ± 0.086
0.346ThrCys: 0.346 ± 0.026
3.01ThrAsp: 3.01 ± 0.074
3.451ThrGlu: 3.451 ± 0.089
2.813ThrPhe: 2.813 ± 0.078
3.619ThrGly: 3.619 ± 0.088
1.529ThrHis: 1.529 ± 0.052
5.178ThrIle: 5.178 ± 0.105
3.732ThrLys: 3.732 ± 0.105
5.529ThrLeu: 5.529 ± 0.087
1.27ThrMet: 1.27 ± 0.038
3.035ThrAsn: 3.035 ± 0.119
2.284ThrPro: 2.284 ± 0.077
2.399ThrGln: 2.399 ± 0.096
1.853ThrArg: 1.853 ± 0.048
3.875ThrSer: 3.875 ± 0.225
3.504ThrThr: 3.504 ± 0.138
3.763ThrVal: 3.763 ± 0.084
0.385ThrTrp: 0.385 ± 0.02
2.263ThrTyr: 2.263 ± 0.07
0.0ThrXaa: 0.0 ± 0.0
Val
3.985ValAla: 3.985 ± 0.073
0.467ValCys: 0.467 ± 0.03
3.903ValAsp: 3.903 ± 0.094
4.286ValGlu: 4.286 ± 0.089
2.666ValPhe: 2.666 ± 0.092
4.167ValGly: 4.167 ± 0.103
1.28ValHis: 1.28 ± 0.046
5.976ValIle: 5.976 ± 0.107
4.864ValLys: 4.864 ± 0.084
5.773ValLeu: 5.773 ± 0.104
1.679ValMet: 1.679 ± 0.053
3.743ValAsn: 3.743 ± 0.126
2.221ValPro: 2.221 ± 0.058
2.115ValGln: 2.115 ± 0.061
2.151ValArg: 2.151 ± 0.066
4.159ValSer: 4.159 ± 0.079
4.064ValThr: 4.064 ± 0.098
4.556ValVal: 4.556 ± 0.102
0.441ValTrp: 0.441 ± 0.026
2.184ValTyr: 2.184 ± 0.064
0.0ValXaa: 0.0 ± 0.0
Trp
0.348TrpAla: 0.348 ± 0.023
0.048TrpCys: 0.048 ± 0.009
0.339TrpAsp: 0.339 ± 0.027
0.344TrpGlu: 0.344 ± 0.025
0.432TrpPhe: 0.432 ± 0.027
0.428TrpGly: 0.428 ± 0.025
0.157TrpHis: 0.157 ± 0.014
0.66TrpIle: 0.66 ± 0.034
0.442TrpLys: 0.442 ± 0.028
0.933TrpLeu: 0.933 ± 0.037
0.236TrpMet: 0.236 ± 0.016
0.41TrpAsn: 0.41 ± 0.023
0.185TrpPro: 0.185 ± 0.018
0.27TrpGln: 0.27 ± 0.021
0.26TrpArg: 0.26 ± 0.02
0.464TrpSer: 0.464 ± 0.027
0.369TrpThr: 0.369 ± 0.024
0.46TrpVal: 0.46 ± 0.028
0.069TrpTrp: 0.069 ± 0.01
0.297TrpTyr: 0.297 ± 0.022
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.161TyrAla: 2.161 ± 0.056
0.309TyrCys: 0.309 ± 0.022
2.654TyrAsp: 2.654 ± 0.066
2.585TyrGlu: 2.585 ± 0.068
1.944TyrPhe: 1.944 ± 0.061
2.431TyrGly: 2.431 ± 0.078
1.129TyrHis: 1.129 ± 0.039
3.248TyrIle: 3.248 ± 0.081
2.677TyrLys: 2.677 ± 0.072
3.843TyrLeu: 3.843 ± 0.087
0.846TyrMet: 0.846 ± 0.033
2.125TyrAsn: 2.125 ± 0.069
1.318TyrPro: 1.318 ± 0.047
1.901TyrGln: 1.901 ± 0.059
1.546TyrArg: 1.546 ± 0.058
2.223TyrSer: 2.223 ± 0.066
2.038TyrThr: 2.038 ± 0.062
2.237TyrVal: 2.237 ± 0.058
0.294TyrTrp: 0.294 ± 0.022
1.708TyrTyr: 1.708 ± 0.059
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2492 proteins (714899 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski