Corynebacterium phage BFK20

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Sasvirus; Corynebacterium virus BFK20

Average proteome isoelectric point is 5.86

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 54 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q3V5G0|Q3V5G0_9CAUD Gp35 OS=Corynebacterium phage BFK20 OX=28358 GN=ORF35 PE=4 SV=1
MM1 pKa = 6.98PAYY4 pKa = 10.25RR5 pKa = 11.84IRR7 pKa = 11.84PGVEE11 pKa = 3.55DD12 pKa = 4.78FNRR15 pKa = 11.84ADD17 pKa = 3.43DD18 pKa = 4.06TEE20 pKa = 4.29RR21 pKa = 11.84QAVEE25 pKa = 3.9LVRR28 pKa = 11.84EE29 pKa = 4.41DD30 pKa = 3.38GVTRR34 pKa = 11.84TFSLEE39 pKa = 3.68VDD41 pKa = 3.55SFEE44 pKa = 4.68GGLYY48 pKa = 10.61FLTGYY53 pKa = 9.45VWEE56 pKa = 4.54DD57 pKa = 3.67TQDD60 pKa = 3.88GEE62 pKa = 4.64HH63 pKa = 7.02LDD65 pKa = 3.74AGGDD69 pKa = 3.87AIEE72 pKa = 4.73CLIDD76 pKa = 3.34AHH78 pKa = 6.86DD79 pKa = 4.88LVDD82 pKa = 5.69EE83 pKa = 4.3LTAWAQAGEE92 pKa = 4.13

Molecular weight: 10.28 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q9MBJ9|Q9MBJ9_9CAUD Gp6 major capsid protein OS=Corynebacterium phage BFK20 OX=28358 GN=ORF6 PE=4 SV=2
MM1 pKa = 7.53SAQDD5 pKa = 3.3KK6 pKa = 10.55ARR8 pKa = 11.84YY9 pKa = 8.92RR10 pKa = 11.84VTLPGSSHH18 pKa = 5.71SVVMQFSSDD27 pKa = 3.67DD28 pKa = 3.85EE29 pKa = 4.38AKK31 pKa = 10.25KK32 pKa = 10.31RR33 pKa = 11.84YY34 pKa = 8.57PGAVRR39 pKa = 11.84VAAPGVGDD47 pKa = 3.9VPTKK51 pKa = 10.59ARR53 pKa = 11.84SARR56 pKa = 11.84GTRR59 pKa = 11.84GSKK62 pKa = 8.19TQTKK66 pKa = 8.96EE67 pKa = 3.52

Molecular weight: 7.17 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

54

0

54

12700

43

1604

235.2

25.68

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.449 ± 0.505

0.646 ± 0.104

6.417 ± 0.221

6.346 ± 0.328

2.74 ± 0.22

8.291 ± 0.536

1.937 ± 0.228

4.26 ± 0.207

4.031 ± 0.199

8.323 ± 0.301

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.417 ± 0.152

3.551 ± 0.297

5.551 ± 0.359

3.724 ± 0.534

5.748 ± 0.399

5.969 ± 0.261

7.291 ± 0.309

7.638 ± 0.289

2.094 ± 0.217

2.575 ± 0.23
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski