Amino acid dipepetide frequency for Corynebacterium phage BFK20

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
8.19AlaAla: 8.19 ± 1.68
0.472AlaCys: 0.472 ± 0.187
5.985AlaAsp: 5.985 ± 0.802
5.276AlaGlu: 5.276 ± 0.553
2.914AlaPhe: 2.914 ± 0.402
9.765AlaGly: 9.765 ± 0.828
1.811AlaHis: 1.811 ± 0.439
5.04AlaIle: 5.04 ± 0.541
3.307AlaLys: 3.307 ± 0.952
8.505AlaLeu: 8.505 ± 1.019
3.859AlaMet: 3.859 ± 0.632
3.622AlaAsn: 3.622 ± 0.543
6.457AlaPro: 6.457 ± 1.257
4.174AlaGln: 4.174 ± 0.489
6.693AlaArg: 6.693 ± 0.682
6.615AlaSer: 6.615 ± 0.924
7.323AlaThr: 7.323 ± 0.979
8.19AlaVal: 8.19 ± 0.864
3.386AlaTrp: 3.386 ± 0.759
2.914AlaTyr: 2.914 ± 0.655
0.0AlaXaa: 0.0 ± 0.0
Cys
1.102CysAla: 1.102 ± 0.336
0.0CysCys: 0.0 ± 0.0
0.394CysAsp: 0.394 ± 0.148
0.236CysGlu: 0.236 ± 0.143
0.0CysPhe: 0.0 ± 0.0
0.63CysGly: 0.63 ± 0.236
0.236CysHis: 0.236 ± 0.15
0.236CysIle: 0.236 ± 0.134
0.315CysLys: 0.315 ± 0.139
0.551CysLeu: 0.551 ± 0.216
0.079CysMet: 0.079 ± 0.089
0.236CysAsn: 0.236 ± 0.141
0.394CysPro: 0.394 ± 0.38
0.394CysGln: 0.394 ± 0.161
0.315CysArg: 0.315 ± 0.135
0.394CysSer: 0.394 ± 0.179
0.315CysThr: 0.315 ± 0.16
0.472CysVal: 0.472 ± 0.216
0.157CysTrp: 0.157 ± 0.102
0.0CysTyr: 0.0 ± 0.0
0.0CysXaa: 0.0 ± 0.0
Asp
6.3AspAla: 6.3 ± 0.796
0.157AspCys: 0.157 ± 0.093
2.756AspAsp: 2.756 ± 0.433
4.882AspGlu: 4.882 ± 0.67
1.575AspPhe: 1.575 ± 0.434
5.748AspGly: 5.748 ± 0.645
1.181AspHis: 1.181 ± 0.374
2.992AspIle: 2.992 ± 0.337
3.465AspLys: 3.465 ± 0.518
6.142AspLeu: 6.142 ± 0.685
1.654AspMet: 1.654 ± 0.28
2.205AspAsn: 2.205 ± 0.442
3.937AspPro: 3.937 ± 0.656
1.89AspGln: 1.89 ± 0.289
2.756AspArg: 2.756 ± 0.455
3.229AspSer: 3.229 ± 0.523
5.906AspThr: 5.906 ± 0.823
4.567AspVal: 4.567 ± 0.669
0.945AspTrp: 0.945 ± 0.224
1.89AspTyr: 1.89 ± 0.321
0.0AspXaa: 0.0 ± 0.0
Glu
6.378GluAla: 6.378 ± 0.491
0.787GluCys: 0.787 ± 0.24
4.174GluAsp: 4.174 ± 0.567
4.252GluGlu: 4.252 ± 0.707
1.969GluPhe: 1.969 ± 0.381
4.882GluGly: 4.882 ± 0.532
2.284GluHis: 2.284 ± 0.417
2.047GluIle: 2.047 ± 0.434
2.52GluLys: 2.52 ± 0.41
5.591GluLeu: 5.591 ± 0.592
1.102GluMet: 1.102 ± 0.281
2.599GluAsn: 2.599 ± 0.56
1.89GluPro: 1.89 ± 0.336
3.15GluGln: 3.15 ± 0.792
5.197GluArg: 5.197 ± 0.696
3.307GluSer: 3.307 ± 0.451
3.15GluThr: 3.15 ± 0.706
5.119GluVal: 5.119 ± 0.741
1.496GluTrp: 1.496 ± 0.342
1.575GluTyr: 1.575 ± 0.302
0.0GluXaa: 0.0 ± 0.0
Phe
2.599PheAla: 2.599 ± 0.421
0.157PheCys: 0.157 ± 0.117
1.654PheAsp: 1.654 ± 0.308
2.362PheGlu: 2.362 ± 0.467
0.945PhePhe: 0.945 ± 0.256
1.732PheGly: 1.732 ± 0.402
0.394PheHis: 0.394 ± 0.175
1.181PheIle: 1.181 ± 0.299
1.102PheLys: 1.102 ± 0.303
2.126PheLeu: 2.126 ± 0.426
0.63PheMet: 0.63 ± 0.221
1.26PheAsn: 1.26 ± 0.363
1.181PhePro: 1.181 ± 0.324
2.047PheGln: 2.047 ± 1.164
1.575PheArg: 1.575 ± 0.294
1.496PheSer: 1.496 ± 0.284
2.284PheThr: 2.284 ± 0.467
1.89PheVal: 1.89 ± 0.427
0.394PheTrp: 0.394 ± 0.221
0.394PheTyr: 0.394 ± 0.165
0.0PheXaa: 0.0 ± 0.0
Gly
8.032GlyAla: 8.032 ± 0.972
0.394GlyCys: 0.394 ± 0.18
5.512GlyAsp: 5.512 ± 0.736
4.725GlyGlu: 4.725 ± 0.492
2.914GlyPhe: 2.914 ± 0.608
7.875GlyGly: 7.875 ± 1.643
1.496GlyHis: 1.496 ± 0.325
4.41GlyIle: 4.41 ± 0.534
3.701GlyLys: 3.701 ± 0.481
7.481GlyLeu: 7.481 ± 0.83
1.732GlyMet: 1.732 ± 0.321
3.386GlyAsn: 3.386 ± 0.672
1.969GlyPro: 1.969 ± 0.502
2.835GlyGln: 2.835 ± 0.484
4.567GlyArg: 4.567 ± 0.627
4.725GlySer: 4.725 ± 0.617
6.142GlyThr: 6.142 ± 1.064
5.748GlyVal: 5.748 ± 0.658
2.284GlyTrp: 2.284 ± 0.474
2.992GlyTyr: 2.992 ± 0.477
0.0GlyXaa: 0.0 ± 0.0
His
1.89HisAla: 1.89 ± 0.393
0.236HisCys: 0.236 ± 0.115
1.024HisAsp: 1.024 ± 0.367
1.024HisGlu: 1.024 ± 0.278
0.315HisPhe: 0.315 ± 0.13
1.969HisGly: 1.969 ± 0.39
0.472HisHis: 0.472 ± 0.189
0.709HisIle: 0.709 ± 0.265
0.787HisLys: 0.787 ± 0.232
2.284HisLeu: 2.284 ± 0.489
0.472HisMet: 0.472 ± 0.166
0.787HisAsn: 0.787 ± 0.254
1.024HisPro: 1.024 ± 0.338
0.236HisGln: 0.236 ± 0.137
1.181HisArg: 1.181 ± 0.371
0.787HisSer: 0.787 ± 0.257
2.284HisThr: 2.284 ± 0.616
1.26HisVal: 1.26 ± 0.336
0.394HisTrp: 0.394 ± 0.17
0.236HisTyr: 0.236 ± 0.155
0.0HisXaa: 0.0 ± 0.0
Ile
4.174IleAla: 4.174 ± 0.499
0.394IleCys: 0.394 ± 0.22
3.622IleAsp: 3.622 ± 0.437
2.992IleGlu: 2.992 ± 0.609
1.339IlePhe: 1.339 ± 0.424
3.229IleGly: 3.229 ± 0.57
0.787IleHis: 0.787 ± 0.242
1.181IleIle: 1.181 ± 0.324
2.52IleLys: 2.52 ± 0.409
4.016IleLeu: 4.016 ± 0.568
0.63IleMet: 0.63 ± 0.243
1.654IleAsn: 1.654 ± 0.37
2.205IlePro: 2.205 ± 0.439
1.417IleGln: 1.417 ± 0.237
2.205IleArg: 2.205 ± 0.418
2.205IleSer: 2.205 ± 0.315
3.544IleThr: 3.544 ± 0.653
2.599IleVal: 2.599 ± 0.327
0.787IleTrp: 0.787 ± 0.231
1.102IleTyr: 1.102 ± 0.316
0.0IleXaa: 0.0 ± 0.0
Lys
4.331LysAla: 4.331 ± 0.646
0.315LysCys: 0.315 ± 0.189
2.205LysAsp: 2.205 ± 0.371
2.126LysGlu: 2.126 ± 0.388
0.945LysPhe: 0.945 ± 0.267
2.52LysGly: 2.52 ± 0.462
0.63LysHis: 0.63 ± 0.191
1.654LysIle: 1.654 ± 0.372
2.047LysLys: 2.047 ± 0.351
3.701LysLeu: 3.701 ± 0.457
0.472LysMet: 0.472 ± 0.183
1.102LysAsn: 1.102 ± 0.227
2.599LysPro: 2.599 ± 0.573
1.102LysGln: 1.102 ± 0.315
2.599LysArg: 2.599 ± 0.375
3.15LysSer: 3.15 ± 0.488
3.701LysThr: 3.701 ± 0.948
3.229LysVal: 3.229 ± 0.498
1.102LysTrp: 1.102 ± 0.256
0.787LysTyr: 0.787 ± 0.19
0.0LysXaa: 0.0 ± 0.0
Leu
8.19LeuAla: 8.19 ± 0.926
0.551LeuCys: 0.551 ± 0.239
8.032LeuAsp: 8.032 ± 0.746
5.355LeuGlu: 5.355 ± 0.511
2.205LeuPhe: 2.205 ± 0.359
7.008LeuGly: 7.008 ± 0.721
1.417LeuHis: 1.417 ± 0.365
3.15LeuIle: 3.15 ± 0.555
2.835LeuLys: 2.835 ± 0.472
6.142LeuLeu: 6.142 ± 0.693
2.284LeuMet: 2.284 ± 0.391
2.835LeuAsn: 2.835 ± 0.588
4.804LeuPro: 4.804 ± 0.609
2.284LeuGln: 2.284 ± 0.358
4.882LeuArg: 4.882 ± 0.851
4.961LeuSer: 4.961 ± 0.649
6.063LeuThr: 6.063 ± 0.799
6.457LeuVal: 6.457 ± 0.774
1.496LeuTrp: 1.496 ± 0.269
2.284LeuTyr: 2.284 ± 0.562
0.0LeuXaa: 0.0 ± 0.0
Met
2.284MetAla: 2.284 ± 0.508
0.315MetCys: 0.315 ± 0.134
1.496MetAsp: 1.496 ± 0.326
0.945MetGlu: 0.945 ± 0.238
0.551MetPhe: 0.551 ± 0.215
1.26MetGly: 1.26 ± 0.349
0.394MetHis: 0.394 ± 0.22
0.866MetIle: 0.866 ± 0.229
1.024MetLys: 1.024 ± 0.29
1.969MetLeu: 1.969 ± 0.552
0.315MetMet: 0.315 ± 0.131
0.394MetAsn: 0.394 ± 0.175
1.654MetPro: 1.654 ± 0.312
0.709MetGln: 0.709 ± 0.201
1.575MetArg: 1.575 ± 0.304
2.599MetSer: 2.599 ± 0.479
2.284MetThr: 2.284 ± 0.498
2.126MetVal: 2.126 ± 0.408
0.709MetTrp: 0.709 ± 0.19
0.709MetTyr: 0.709 ± 0.205
0.0MetXaa: 0.0 ± 0.0
Asn
4.961AsnAla: 4.961 ± 0.864
0.157AsnCys: 0.157 ± 0.177
1.417AsnAsp: 1.417 ± 0.314
1.575AsnGlu: 1.575 ± 0.466
0.709AsnPhe: 0.709 ± 0.227
3.386AsnGly: 3.386 ± 0.531
0.63AsnHis: 0.63 ± 0.257
1.181AsnIle: 1.181 ± 0.315
1.339AsnLys: 1.339 ± 0.345
3.071AsnLeu: 3.071 ± 0.64
0.63AsnMet: 0.63 ± 0.19
1.26AsnAsn: 1.26 ± 0.288
3.386AsnPro: 3.386 ± 0.398
2.284AsnGln: 2.284 ± 1.022
2.205AsnArg: 2.205 ± 0.285
1.575AsnSer: 1.575 ± 0.409
2.126AsnThr: 2.126 ± 0.382
2.205AsnVal: 2.205 ± 0.395
0.315AsnTrp: 0.315 ± 0.183
1.102AsnTyr: 1.102 ± 0.22
0.0AsnXaa: 0.0 ± 0.0
Pro
5.985ProAla: 5.985 ± 0.731
0.472ProCys: 0.472 ± 0.231
2.441ProAsp: 2.441 ± 0.52
4.567ProGlu: 4.567 ± 0.56
1.102ProPhe: 1.102 ± 0.292
5.827ProGly: 5.827 ± 0.872
1.575ProHis: 1.575 ± 0.348
2.52ProIle: 2.52 ± 0.43
1.89ProLys: 1.89 ± 0.353
2.52ProLeu: 2.52 ± 0.463
1.575ProMet: 1.575 ± 0.321
1.969ProAsn: 1.969 ± 0.89
2.914ProPro: 2.914 ± 0.63
2.914ProGln: 2.914 ± 1.483
3.15ProArg: 3.15 ± 0.567
3.859ProSer: 3.859 ± 0.752
2.992ProThr: 2.992 ± 0.518
4.567ProVal: 4.567 ± 0.605
1.417ProTrp: 1.417 ± 0.363
1.26ProTyr: 1.26 ± 0.298
0.0ProXaa: 0.0 ± 0.0
Gln
5.512GlnAla: 5.512 ± 1.075
0.157GlnCys: 0.157 ± 0.098
2.284GlnAsp: 2.284 ± 0.407
1.89GlnGlu: 1.89 ± 0.694
1.969GlnPhe: 1.969 ± 0.924
3.15GlnGly: 3.15 ± 0.652
0.394GlnHis: 0.394 ± 0.228
1.89GlnIle: 1.89 ± 0.539
0.787GlnLys: 0.787 ± 0.212
2.205GlnLeu: 2.205 ± 0.393
0.787GlnMet: 0.787 ± 0.22
0.394GlnAsn: 0.394 ± 0.162
3.386GlnPro: 3.386 ± 1.395
2.126GlnGln: 2.126 ± 1.248
1.811GlnArg: 1.811 ± 0.368
1.811GlnSer: 1.811 ± 0.356
2.362GlnThr: 2.362 ± 0.43
2.599GlnVal: 2.599 ± 0.382
0.866GlnTrp: 0.866 ± 0.285
0.866GlnTyr: 0.866 ± 0.296
0.0GlnXaa: 0.0 ± 0.0
Arg
8.111ArgAla: 8.111 ± 0.667
0.315ArgCys: 0.315 ± 0.169
3.937ArgAsp: 3.937 ± 0.5
5.433ArgGlu: 5.433 ± 0.747
1.102ArgPhe: 1.102 ± 0.278
3.465ArgGly: 3.465 ± 0.521
0.866ArgHis: 0.866 ± 0.195
2.835ArgIle: 2.835 ± 0.483
2.284ArgLys: 2.284 ± 0.467
4.016ArgLeu: 4.016 ± 0.459
1.654ArgMet: 1.654 ± 0.499
1.811ArgAsn: 1.811 ± 0.389
3.701ArgPro: 3.701 ± 0.559
1.417ArgGln: 1.417 ± 0.325
2.914ArgArg: 2.914 ± 0.561
2.756ArgSer: 2.756 ± 0.567
3.859ArgThr: 3.859 ± 0.538
5.04ArgVal: 5.04 ± 0.597
0.472ArgTrp: 0.472 ± 0.185
1.417ArgTyr: 1.417 ± 0.341
0.0ArgXaa: 0.0 ± 0.0
Ser
5.591SerAla: 5.591 ± 1.013
0.315SerCys: 0.315 ± 0.163
3.15SerAsp: 3.15 ± 0.484
4.489SerGlu: 4.489 ± 0.799
1.732SerPhe: 1.732 ± 0.294
4.882SerGly: 4.882 ± 0.883
0.866SerHis: 0.866 ± 0.221
2.441SerIle: 2.441 ± 0.411
2.756SerLys: 2.756 ± 0.37
5.433SerLeu: 5.433 ± 0.609
1.024SerMet: 1.024 ± 0.308
2.677SerAsn: 2.677 ± 0.48
2.677SerPro: 2.677 ± 0.427
2.362SerGln: 2.362 ± 0.436
3.15SerArg: 3.15 ± 0.496
3.15SerSer: 3.15 ± 0.469
4.725SerThr: 4.725 ± 0.575
4.882SerVal: 4.882 ± 0.626
1.181SerTrp: 1.181 ± 0.367
2.205SerTyr: 2.205 ± 0.412
0.0SerXaa: 0.0 ± 0.0
Thr
8.583ThrAla: 8.583 ± 0.909
0.63ThrCys: 0.63 ± 0.313
4.567ThrAsp: 4.567 ± 0.717
3.386ThrGlu: 3.386 ± 0.46
2.205ThrPhe: 2.205 ± 0.474
5.985ThrGly: 5.985 ± 0.776
1.575ThrHis: 1.575 ± 0.468
2.441ThrIle: 2.441 ± 0.474
2.756ThrLys: 2.756 ± 0.679
6.93ThrLeu: 6.93 ± 0.602
1.969ThrMet: 1.969 ± 0.436
2.599ThrAsn: 2.599 ± 0.806
4.961ThrPro: 4.961 ± 0.55
1.811ThrGln: 1.811 ± 0.349
3.465ThrArg: 3.465 ± 0.508
4.804ThrSer: 4.804 ± 1.186
3.859ThrThr: 3.859 ± 0.436
7.166ThrVal: 7.166 ± 0.712
1.417ThrTrp: 1.417 ± 0.289
1.811ThrTyr: 1.811 ± 0.421
0.0ThrXaa: 0.0 ± 0.0
Val
7.638ValAla: 7.638 ± 0.68
0.315ValCys: 0.315 ± 0.14
5.67ValAsp: 5.67 ± 0.705
5.355ValGlu: 5.355 ± 0.617
1.654ValPhe: 1.654 ± 0.297
6.063ValGly: 6.063 ± 0.66
1.339ValHis: 1.339 ± 0.319
3.307ValIle: 3.307 ± 0.42
3.229ValLys: 3.229 ± 0.459
5.512ValLeu: 5.512 ± 0.928
1.969ValMet: 1.969 ± 0.385
3.15ValAsn: 3.15 ± 0.468
4.41ValPro: 4.41 ± 0.511
2.914ValGln: 2.914 ± 0.464
3.78ValArg: 3.78 ± 0.67
5.197ValSer: 5.197 ± 0.597
6.93ValThr: 6.93 ± 0.733
5.276ValVal: 5.276 ± 0.818
1.417ValTrp: 1.417 ± 0.28
1.26ValTyr: 1.26 ± 0.265
0.0ValXaa: 0.0 ± 0.0
Trp
2.047TrpAla: 2.047 ± 0.456
0.079TrpCys: 0.079 ± 0.072
2.047TrpAsp: 2.047 ± 0.67
1.732TrpGlu: 1.732 ± 0.396
0.63TrpPhe: 0.63 ± 0.247
1.181TrpGly: 1.181 ± 0.345
0.236TrpHis: 0.236 ± 0.141
1.102TrpIle: 1.102 ± 0.229
0.551TrpLys: 0.551 ± 0.217
2.205TrpLeu: 2.205 ± 0.27
0.709TrpMet: 0.709 ± 0.266
0.866TrpAsn: 0.866 ± 0.293
0.866TrpPro: 0.866 ± 0.238
0.63TrpGln: 0.63 ± 0.224
1.181TrpArg: 1.181 ± 0.276
1.26TrpSer: 1.26 ± 0.326
1.575TrpThr: 1.575 ± 0.365
1.339TrpVal: 1.339 ± 0.336
0.236TrpTrp: 0.236 ± 0.131
0.472TrpTyr: 0.472 ± 0.182
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.599TyrAla: 2.599 ± 0.514
0.236TyrCys: 0.236 ± 0.146
1.811TyrAsp: 1.811 ± 0.408
0.866TyrGlu: 0.866 ± 0.229
0.63TyrPhe: 0.63 ± 0.186
2.362TyrGly: 2.362 ± 0.376
0.787TyrHis: 0.787 ± 0.218
1.417TyrIle: 1.417 ± 0.283
1.102TyrLys: 1.102 ± 0.304
2.835TyrLeu: 2.835 ± 0.632
0.551TyrMet: 0.551 ± 0.232
0.709TyrAsn: 0.709 ± 0.197
1.102TyrPro: 1.102 ± 0.312
0.551TyrGln: 0.551 ± 0.186
2.284TyrArg: 2.284 ± 0.408
1.811TyrSer: 1.811 ± 0.466
1.496TyrThr: 1.496 ± 0.342
1.654TyrVal: 1.654 ± 0.43
0.472TyrTrp: 0.472 ± 0.183
0.472TyrTyr: 0.472 ± 0.223
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 54 proteins (12700 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski