Clostridium tepidiprofundi DSM 19306

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Clostridium; Clostridium tepidiprofundi

Average proteome isoelectric point is 6.95

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2724 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A151B4R4|A0A151B4R4_9CLOT Ribosomal RNA large subunit methyltransferase H OS=Clostridium tepidiprofundi DSM 19306 OX=1121338 GN=rlmH PE=3 SV=1
MM1 pKa = 7.8EE2 pKa = 4.91EE3 pKa = 4.31NKK5 pKa = 10.59SKK7 pKa = 10.9SNEE10 pKa = 3.92YY11 pKa = 10.38KK12 pKa = 10.73EE13 pKa = 4.41NEE15 pKa = 4.38CEE17 pKa = 4.14VGHH20 pKa = 6.36SEE22 pKa = 4.93EE23 pKa = 4.19EE24 pKa = 4.43FEE26 pKa = 5.22ALTVDD31 pKa = 5.44LEE33 pKa = 4.41DD34 pKa = 3.68EE35 pKa = 4.36EE36 pKa = 5.85GNIIHH41 pKa = 6.87CEE43 pKa = 3.82IIDD46 pKa = 3.74GFEE49 pKa = 4.23FNDD52 pKa = 3.45DD53 pKa = 3.45EE54 pKa = 4.84YY55 pKa = 11.81AVVQNPQDD63 pKa = 3.36EE64 pKa = 4.56TLYY67 pKa = 10.2LFKK70 pKa = 11.04VIGDD74 pKa = 4.03DD75 pKa = 3.66EE76 pKa = 4.75VGEE79 pKa = 4.32LVLPDD84 pKa = 4.08DD85 pKa = 5.75DD86 pKa = 3.9EE87 pKa = 4.94FEE89 pKa = 4.32RR90 pKa = 11.84ARR92 pKa = 11.84EE93 pKa = 4.04YY94 pKa = 11.19YY95 pKa = 9.93EE96 pKa = 4.23SLIEE100 pKa = 4.41SEE102 pKa = 4.47EE103 pKa = 3.97

Molecular weight: 12.07 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A151B5L9|A0A151B5L9_9CLOT DD-transpeptidase OS=Clostridium tepidiprofundi DSM 19306 OX=1121338 GN=ponA PE=3 SV=1
MM1 pKa = 7.37ARR3 pKa = 11.84KK4 pKa = 9.48ALIQKK9 pKa = 8.04WNKK12 pKa = 7.43EE13 pKa = 4.11PKK15 pKa = 9.1YY16 pKa = 9.38STRR19 pKa = 11.84AYY21 pKa = 8.1TRR23 pKa = 11.84CRR25 pKa = 11.84ICGRR29 pKa = 11.84PHH31 pKa = 6.44AVLRR35 pKa = 11.84KK36 pKa = 9.63YY37 pKa = 9.83GICRR41 pKa = 11.84ICFRR45 pKa = 11.84EE46 pKa = 3.97LAYY49 pKa = 9.88KK50 pKa = 10.14GQIPGCRR57 pKa = 11.84KK58 pKa = 10.14ASWW61 pKa = 3.01

Molecular weight: 7.23 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2724

0

2724

818141

29

2939

300.3

34.14

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.228 ± 0.044

1.262 ± 0.02

5.62 ± 0.038

7.211 ± 0.051

4.389 ± 0.035

6.033 ± 0.043

1.391 ± 0.018

10.549 ± 0.057

9.647 ± 0.053

8.683 ± 0.057

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.618 ± 0.024

6.75 ± 0.052

2.683 ± 0.028

2.209 ± 0.022

3.548 ± 0.031

6.083 ± 0.034

4.645 ± 0.048

6.374 ± 0.046

0.669 ± 0.014

4.407 ± 0.037
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski