Streptomyces cinnamoneus (Streptoverticillium cinnamoneum)
Average proteome isoelectric point is 6.64
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5593 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2G1XPG7|A0A2G1XPG7_STRCJ Uncharacterized protein OS=Streptomyces cinnamoneus OX=53446 GN=BLA24_03300 PE=4 SV=1
MM1 pKa = 7.57 RR2 pKa = 11.84 LIVAAFQDD10 pKa = 4.17 VPEE13 pKa = 4.35 EE14 pKa = 3.93 TWGRR18 pKa = 11.84 SAGEE22 pKa = 4.33 LEE24 pKa = 4.03 WDD26 pKa = 3.12 CWEE29 pKa = 4.36 TVEE32 pKa = 5.76 HH33 pKa = 6.59 LADD36 pKa = 5.71 DD37 pKa = 4.4 MFCYY41 pKa = 10.41 ALQLSAPNPPLDD53 pKa = 3.84 SYY55 pKa = 11.95 VPTLMTCQRR64 pKa = 11.84 DD65 pKa = 3.61 GG66 pKa = 3.49
Molecular weight: 7.52 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.783
IPC2_protein 3.948
IPC_protein 3.821
Toseland 3.643
ProMoST 3.999
Dawson 3.821
Bjellqvist 3.973
Wikipedia 3.757
Rodwell 3.668
Grimsley 3.567
Solomon 3.795
Lehninger 3.745
Nozaki 3.948
DTASelect 4.113
Thurlkill 3.706
EMBOSS 3.757
Sillero 3.948
Patrickios 0.693
IPC_peptide 3.783
IPC2_peptide 3.923
IPC2.peptide.svr19 3.859
Protein with the highest isoelectric point:
>tr|A0A2G1XHN3|A0A2G1XHN3_STRCJ Uncharacterized protein OS=Streptomyces cinnamoneus OX=53446 GN=BLA24_18335 PE=4 SV=1
MM1 pKa = 7.69 SKK3 pKa = 9.0 RR4 pKa = 11.84 TFQPNNRR11 pKa = 11.84 RR12 pKa = 11.84 RR13 pKa = 11.84 AKK15 pKa = 8.7 THH17 pKa = 5.15 GFRR20 pKa = 11.84 LRR22 pKa = 11.84 MRR24 pKa = 11.84 TRR26 pKa = 11.84 AGRR29 pKa = 11.84 AILASRR35 pKa = 11.84 RR36 pKa = 11.84 GKK38 pKa = 10.36 GRR40 pKa = 11.84 ARR42 pKa = 11.84 LSAA45 pKa = 3.91
Molecular weight: 5.25 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.527
IPC2_protein 11.316
IPC_protein 12.925
Toseland 13.086
ProMoST 13.583
Dawson 13.086
Bjellqvist 13.086
Wikipedia 13.554
Rodwell 12.676
Grimsley 13.13
Solomon 13.583
Lehninger 13.481
Nozaki 13.086
DTASelect 13.086
Thurlkill 13.086
EMBOSS 13.583
Sillero 13.086
Patrickios 12.398
IPC_peptide 13.583
IPC2_peptide 12.574
IPC2.peptide.svr19 9.243
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5593
0
5593
1876415
28
5768
335.5
35.83
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
14.005 ± 0.05
0.85 ± 0.008
5.85 ± 0.024
5.736 ± 0.032
2.704 ± 0.019
9.756 ± 0.035
2.351 ± 0.012
2.777 ± 0.023
2.17 ± 0.029
10.344 ± 0.04
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.726 ± 0.012
1.614 ± 0.016
6.262 ± 0.034
2.629 ± 0.018
8.352 ± 0.035
4.819 ± 0.024
5.89 ± 0.026
8.68 ± 0.036
1.494 ± 0.013
1.991 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here