Acinetobacter sp.
Average proteome isoelectric point is 6.61
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3709 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A519JEX7|A0A519JEX7_ACISP Pyridoxal phosphate homeostasis protein OS=Acinetobacter sp. OX=472 GN=EON51_03365 PE=3 SV=1
MM1 pKa = 7.48 LKK3 pKa = 10.41 KK4 pKa = 10.06 IALATALIASLGTAHH19 pKa = 8.12 AYY21 pKa = 7.95 QAEE24 pKa = 4.41 VGGTIALVDD33 pKa = 4.34 PDD35 pKa = 4.66 NGDD38 pKa = 3.19 TSTGFGIDD46 pKa = 2.85 ATYY49 pKa = 11.22 YY50 pKa = 10.13 FDD52 pKa = 5.41 SVRR55 pKa = 11.84 LKK57 pKa = 10.58 NDD59 pKa = 3.68 PYY61 pKa = 11.63 NEE63 pKa = 3.9 AAFLNRR69 pKa = 11.84 ASNLNAAFSYY79 pKa = 10.55 IDD81 pKa = 3.49 YY82 pKa = 10.9 DD83 pKa = 3.85 LYY85 pKa = 11.64 EE86 pKa = 4.31 NTTFNAGIEE95 pKa = 4.26 YY96 pKa = 9.24 FVPNSDD102 pKa = 4.23 FYY104 pKa = 11.11 ISGSLGQVSEE114 pKa = 4.21 EE115 pKa = 4.33 TEE117 pKa = 4.05 GFEE120 pKa = 6.02 DD121 pKa = 5.87 FDD123 pKa = 3.96 TTLYY127 pKa = 10.78 AAEE130 pKa = 4.85 LGYY133 pKa = 11.01 LPIPGLLLAVGLAGYY148 pKa = 10.87 DD149 pKa = 3.4 NDD151 pKa = 5.12 RR152 pKa = 11.84 DD153 pKa = 4.16 DD154 pKa = 5.84 GVDD157 pKa = 3.11 PTLRR161 pKa = 11.84 AKK163 pKa = 10.45 YY164 pKa = 6.91 VTRR167 pKa = 11.84 VGQYY171 pKa = 11.34 DD172 pKa = 3.65 MNFEE176 pKa = 4.38 AGANFGDD183 pKa = 4.0 LDD185 pKa = 4.62 AYY187 pKa = 9.77 TLGTDD192 pKa = 4.46 LYY194 pKa = 11.2 LDD196 pKa = 3.61 NTFSAGLGFADD207 pKa = 3.32 SDD209 pKa = 3.89 VRR211 pKa = 11.84 NSDD214 pKa = 3.29 VFTIRR219 pKa = 11.84 AKK221 pKa = 10.69 KK222 pKa = 9.98 FLNQQVSLEE231 pKa = 3.95 GSINFADD238 pKa = 4.03 AGNIFALRR246 pKa = 11.84 GAYY249 pKa = 9.69 RR250 pKa = 11.84 FF251 pKa = 3.75
Molecular weight: 27.29 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.727
IPC2_protein 3.923
IPC_protein 3.935
Toseland 3.706
ProMoST 4.101
Dawson 3.923
Bjellqvist 4.075
Wikipedia 3.872
Rodwell 3.757
Grimsley 3.605
Solomon 3.923
Lehninger 3.872
Nozaki 4.037
DTASelect 4.304
Thurlkill 3.757
EMBOSS 3.884
Sillero 4.05
Patrickios 1.214
IPC_peptide 3.91
IPC2_peptide 4.024
IPC2.peptide.svr19 3.924
Protein with the highest isoelectric point:
>tr|A0A2D7VG37|A0A2D7VG37_ACISP 50S ribosomal protein L28 OS=Acinetobacter sp. OX=472 GN=rpmB PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.55 RR3 pKa = 11.84 TFQPSEE9 pKa = 3.97 LKK11 pKa = 10.13 RR12 pKa = 11.84 KK13 pKa = 8.98 RR14 pKa = 11.84 VHH16 pKa = 6.36 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.4 AGRR28 pKa = 11.84 QVLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.18 GRR39 pKa = 11.84 HH40 pKa = 5.1 SLTVV44 pKa = 3.06
Molecular weight: 5.18 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.402
IPC2_protein 10.862
IPC_protein 12.442
Toseland 12.618
ProMoST 13.1
Dawson 12.618
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.31
Grimsley 12.647
Solomon 13.1
Lehninger 13.013
Nozaki 12.618
DTASelect 12.603
Thurlkill 12.618
EMBOSS 13.115
Sillero 12.618
Patrickios 12.047
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 9.077
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3709
0
3709
1144572
25
2004
308.6
34.46
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.474 ± 0.047
0.908 ± 0.014
5.153 ± 0.035
5.828 ± 0.044
4.465 ± 0.033
6.62 ± 0.043
2.191 ± 0.023
6.988 ± 0.037
6.098 ± 0.036
10.197 ± 0.047
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.502 ± 0.022
4.813 ± 0.033
3.903 ± 0.026
4.997 ± 0.042
4.12 ± 0.033
6.136 ± 0.032
5.391 ± 0.033
6.556 ± 0.034
1.235 ± 0.016
3.424 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here