Altererythrobacter confluentis
Average proteome isoelectric point is 6.21
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2678 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A6L7GKP7|A0A6L7GKP7_9SPHN Prolyl oligopeptidase family serine peptidase OS=Altererythrobacter confluentis OX=1849021 GN=GRI44_10820 PE=3 SV=1
MM1 pKa = 7.45 TGLAVLIPLALCMGALGLAACFWAMKK27 pKa = 9.95 SGQYY31 pKa = 10.89 DD32 pKa = 3.83 DD33 pKa = 5.82 LDD35 pKa = 4.27 GAAHH39 pKa = 7.08 RR40 pKa = 11.84 VLLDD44 pKa = 4.96 DD45 pKa = 5.17 EE46 pKa = 5.22 DD47 pKa = 5.68 LEE49 pKa = 4.5
Molecular weight: 5.2 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.761
IPC2_protein 3.923
IPC_protein 3.783
Toseland 3.592
ProMoST 3.923
Dawson 3.808
Bjellqvist 4.101
Wikipedia 3.808
Rodwell 3.63
Grimsley 3.516
Solomon 3.77
Lehninger 3.732
Nozaki 3.961
DTASelect 4.19
Thurlkill 3.681
EMBOSS 3.808
Sillero 3.923
Patrickios 1.926
IPC_peptide 3.77
IPC2_peptide 3.884
IPC2.peptide.svr19 3.888
Protein with the highest isoelectric point:
>tr|A0A6L7GHN9|A0A6L7GHN9_9SPHN Prolyl oligopeptidase family serine peptidase OS=Altererythrobacter confluentis OX=1849021 GN=GRI44_06695 PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.61 RR3 pKa = 11.84 TFQPSNLVRR12 pKa = 11.84 ARR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.89 GFFARR21 pKa = 11.84 KK22 pKa = 8.02 ATVGGRR28 pKa = 11.84 KK29 pKa = 8.67 ILRR32 pKa = 11.84 ARR34 pKa = 11.84 RR35 pKa = 11.84 ARR37 pKa = 11.84 GRR39 pKa = 11.84 KK40 pKa = 9.02 KK41 pKa = 10.61 LSAA44 pKa = 4.03
Molecular weight: 5.11 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.242
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.676
Grimsley 13.042
Solomon 13.495
Lehninger 13.408
Nozaki 13.013
DTASelect 12.998
Thurlkill 13.013
EMBOSS 13.51
Sillero 13.013
Patrickios 12.398
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.177
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2678
0
2678
877066
38
2912
327.5
35.47
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.774 ± 0.066
0.839 ± 0.013
6.155 ± 0.037
5.677 ± 0.046
3.651 ± 0.026
8.698 ± 0.052
1.974 ± 0.025
5.314 ± 0.034
3.298 ± 0.041
9.71 ± 0.052
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.666 ± 0.025
2.803 ± 0.029
4.993 ± 0.038
3.479 ± 0.026
6.794 ± 0.041
5.613 ± 0.035
5.204 ± 0.042
6.809 ± 0.033
1.381 ± 0.022
2.168 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here