Betaproteobacteria bacterium SCGC AG-212-J23

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; unclassified Betaproteobacteria

Average proteome isoelectric point is 7.45

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2067 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A177R0S4|A0A177R0S4_9PROT Enoyl-CoA hydratase OS=Betaproteobacteria bacterium SCGC AG-212-J23 OX=1799662 GN=AYO46_11035 PE=3 SV=1
MM1 pKa = 7.66TEE3 pKa = 3.78NQVQYY8 pKa = 8.74KK9 pKa = 8.09TWMCLICGFVYY20 pKa = 10.69DD21 pKa = 4.88EE22 pKa = 4.62AAGLPDD28 pKa = 3.87EE29 pKa = 5.79GIAPGTRR36 pKa = 11.84WDD38 pKa = 4.21DD39 pKa = 3.52VPMNWTCPEE48 pKa = 3.86CGARR52 pKa = 11.84KK53 pKa = 9.51EE54 pKa = 3.97DD55 pKa = 3.81FEE57 pKa = 4.37MVEE60 pKa = 4.0II61 pKa = 4.86

Molecular weight: 6.99 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A177QU73|A0A177QU73_9PROT CMD domain-containing protein (Fragment) OS=Betaproteobacteria bacterium SCGC AG-212-J23 OX=1799662 GN=AYO46_10620 PE=4 SV=1
MM1 pKa = 7.41SFAEE5 pKa = 5.72KK6 pKa = 10.18LVAWQRR12 pKa = 11.84RR13 pKa = 11.84QGRR16 pKa = 11.84HH17 pKa = 5.54DD18 pKa = 4.92LPWQGTRR25 pKa = 11.84DD26 pKa = 3.83PYY28 pKa = 10.85RR29 pKa = 11.84IWLSEE34 pKa = 3.93VMLQQTQVATVIPYY48 pKa = 8.3YY49 pKa = 10.73EE50 pKa = 4.53RR51 pKa = 11.84FLKK54 pKa = 10.24KK55 pKa = 10.08YY56 pKa = 9.9PSVEE60 pKa = 4.06FLASASQEE68 pKa = 3.88EE69 pKa = 4.68VLQLWSGLGYY79 pKa = 9.3YY80 pKa = 10.52ARR82 pKa = 11.84GRR84 pKa = 11.84NLHH87 pKa = 6.31AAAKK91 pKa = 9.25EE92 pKa = 3.64IARR95 pKa = 11.84NGFPKK100 pKa = 10.46DD101 pKa = 3.1IAKK104 pKa = 10.49LPGVGRR110 pKa = 11.84STAAAIAVFAFGEE123 pKa = 4.21RR124 pKa = 11.84AAIMDD129 pKa = 4.19GNAKK133 pKa = 9.74RR134 pKa = 11.84VLARR138 pKa = 11.84VFGVPDD144 pKa = 4.6PDD146 pKa = 3.06WDD148 pKa = 3.75LAEE151 pKa = 5.21RR152 pKa = 11.84EE153 pKa = 4.4LPQKK157 pKa = 10.66DD158 pKa = 3.5VAVYY162 pKa = 8.13TQALMDD168 pKa = 4.99LGATVCTRR176 pKa = 11.84RR177 pKa = 11.84PDD179 pKa = 3.55CARR182 pKa = 11.84CPVQRR187 pKa = 11.84GCVARR192 pKa = 11.84RR193 pKa = 11.84KK194 pKa = 10.4DD195 pKa = 3.56RR196 pKa = 11.84VAEE199 pKa = 4.11LPAPRR204 pKa = 11.84VRR206 pKa = 11.84KK207 pKa = 9.45VLPLRR212 pKa = 11.84HH213 pKa = 5.82ATWSILKK220 pKa = 10.56RR221 pKa = 11.84NDD223 pKa = 3.12QILLEE228 pKa = 4.4RR229 pKa = 11.84RR230 pKa = 11.84PSSGIWGGLWCFPEE244 pKa = 4.07AAKK247 pKa = 10.59PGGRR251 pKa = 11.84KK252 pKa = 9.13LPPIEE257 pKa = 5.19HH258 pKa = 6.82GFTHH262 pKa = 6.88FRR264 pKa = 11.84LKK266 pKa = 10.03IQPVLRR272 pKa = 11.84EE273 pKa = 3.7VRR275 pKa = 11.84KK276 pKa = 10.09RR277 pKa = 11.84IPAGPAQRR285 pKa = 11.84WMRR288 pKa = 11.84LADD291 pKa = 3.5VASAAVPTPVRR302 pKa = 11.84RR303 pKa = 11.84LISSLRR309 pKa = 3.54

Molecular weight: 34.93 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2067

0

2067

629598

37

2126

304.6

33.29

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.175 ± 0.071

0.908 ± 0.018

5.001 ± 0.03

5.885 ± 0.051

3.846 ± 0.035

8.341 ± 0.052

2.04 ± 0.026

4.689 ± 0.037

4.442 ± 0.051

10.374 ± 0.06

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.402 ± 0.028

2.597 ± 0.033

5.158 ± 0.036

3.248 ± 0.032

7.201 ± 0.06

5.213 ± 0.04

4.793 ± 0.059

7.768 ± 0.041

1.414 ± 0.024

2.504 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski