Amino acid dipepetide frequency for Betaproteobacteria bacterium SCGC AG-212-J23

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
16.8AlaAla: 16.8 ± 0.267
1.078AlaCys: 1.078 ± 0.044
5.751AlaAsp: 5.751 ± 0.09
7.049AlaGlu: 7.049 ± 0.118
4.389AlaPhe: 4.389 ± 0.086
10.494AlaGly: 10.494 ± 0.127
2.09AlaHis: 2.09 ± 0.058
5.626AlaIle: 5.626 ± 0.104
5.654AlaLys: 5.654 ± 0.106
13.615AlaLeu: 13.615 ± 0.197
3.277AlaMet: 3.277 ± 0.075
2.756AlaAsn: 2.756 ± 0.076
5.473AlaPro: 5.473 ± 0.123
4.292AlaGln: 4.292 ± 0.088
8.671AlaArg: 8.671 ± 0.147
6.218AlaSer: 6.218 ± 0.102
5.434AlaThr: 5.434 ± 0.099
8.675AlaVal: 8.675 ± 0.135
1.707AlaTrp: 1.707 ± 0.057
2.702AlaTyr: 2.702 ± 0.068
0.0AlaXaa: 0.0 ± 0.0
Cys
1.015CysAla: 1.015 ± 0.037
0.118CysCys: 0.118 ± 0.014
0.542CysAsp: 0.542 ± 0.032
0.484CysGlu: 0.484 ± 0.027
0.361CysPhe: 0.361 ± 0.028
0.993CysGly: 0.993 ± 0.039
0.292CysHis: 0.292 ± 0.028
0.438CysIle: 0.438 ± 0.031
0.276CysLys: 0.276 ± 0.022
0.761CysLeu: 0.761 ± 0.038
0.181CysMet: 0.181 ± 0.02
0.245CysAsn: 0.245 ± 0.02
0.434CysPro: 0.434 ± 0.031
0.222CysGln: 0.222 ± 0.016
0.646CysArg: 0.646 ± 0.034
0.51CysSer: 0.51 ± 0.034
0.454CysThr: 0.454 ± 0.031
0.758CysVal: 0.758 ± 0.036
0.098CysTrp: 0.098 ± 0.014
0.249CysTyr: 0.249 ± 0.021
0.0CysXaa: 0.0 ± 0.0
Asp
6.39AspAla: 6.39 ± 0.101
0.494AspCys: 0.494 ± 0.03
2.333AspAsp: 2.333 ± 0.068
3.382AspGlu: 3.382 ± 0.074
2.243AspPhe: 2.243 ± 0.059
4.134AspGly: 4.134 ± 0.087
0.904AspHis: 0.904 ± 0.041
2.328AspIle: 2.328 ± 0.065
1.919AspLys: 1.919 ± 0.058
5.284AspLeu: 5.284 ± 0.096
1.061AspMet: 1.061 ± 0.042
1.077AspAsn: 1.077 ± 0.044
3.116AspPro: 3.116 ± 0.073
1.317AspGln: 1.317 ± 0.046
3.513AspArg: 3.513 ± 0.073
2.1AspSer: 2.1 ± 0.06
2.057AspThr: 2.057 ± 0.059
4.041AspVal: 4.041 ± 0.086
0.888AspTrp: 0.888 ± 0.042
1.431AspTyr: 1.431 ± 0.049
0.0AspXaa: 0.0 ± 0.0
Glu
6.889GluAla: 6.889 ± 0.143
0.419GluCys: 0.419 ± 0.03
2.294GluAsp: 2.294 ± 0.054
3.332GluGlu: 3.332 ± 0.093
2.152GluPhe: 2.152 ± 0.058
4.069GluGly: 4.069 ± 0.099
1.307GluHis: 1.307 ± 0.055
3.294GluIle: 3.294 ± 0.083
3.412GluLys: 3.412 ± 0.087
6.169GluLeu: 6.169 ± 0.106
1.501GluMet: 1.501 ± 0.052
1.564GluAsn: 1.564 ± 0.05
2.646GluPro: 2.646 ± 0.072
2.251GluGln: 2.251 ± 0.072
5.531GluArg: 5.531 ± 0.119
2.803GluSer: 2.803 ± 0.069
2.776GluThr: 2.776 ± 0.068
4.293GluVal: 4.293 ± 0.089
0.801GluTrp: 0.801 ± 0.037
1.347GluTyr: 1.347 ± 0.046
0.0GluXaa: 0.0 ± 0.0
Phe
4.684PheAla: 4.684 ± 0.102
0.4PheCys: 0.4 ± 0.025
2.365PheAsp: 2.365 ± 0.062
2.241PheGlu: 2.241 ± 0.064
1.53PhePhe: 1.53 ± 0.052
3.539PheGly: 3.539 ± 0.078
0.739PheHis: 0.739 ± 0.035
1.576PheIle: 1.576 ± 0.051
1.321PheLys: 1.321 ± 0.046
3.634PheLeu: 3.634 ± 0.098
0.82PheMet: 0.82 ± 0.04
1.036PheAsn: 1.036 ± 0.047
1.819PhePro: 1.819 ± 0.058
1.074PheGln: 1.074 ± 0.041
2.475PheArg: 2.475 ± 0.056
2.511PheSer: 2.511 ± 0.064
2.025PheThr: 2.025 ± 0.063
3.085PheVal: 3.085 ± 0.085
0.572PheTrp: 0.572 ± 0.034
1.015PheTyr: 1.015 ± 0.041
0.0PheXaa: 0.0 ± 0.0
Gly
9.201GlyAla: 9.201 ± 0.15
0.926GlyCys: 0.926 ± 0.038
3.736GlyAsp: 3.736 ± 0.086
5.014GlyGlu: 5.014 ± 0.089
3.496GlyPhe: 3.496 ± 0.085
6.968GlyGly: 6.968 ± 0.133
1.709GlyHis: 1.709 ± 0.055
4.354GlyIle: 4.354 ± 0.089
4.395GlyLys: 4.395 ± 0.094
8.069GlyLeu: 8.069 ± 0.132
2.352GlyMet: 2.352 ± 0.065
2.182GlyAsn: 2.182 ± 0.066
3.096GlyPro: 3.096 ± 0.068
2.537GlyGln: 2.537 ± 0.075
5.726GlyArg: 5.726 ± 0.114
4.593GlySer: 4.593 ± 0.085
4.414GlyThr: 4.414 ± 0.112
6.763GlyVal: 6.763 ± 0.104
1.361GlyTrp: 1.361 ± 0.049
2.522GlyTyr: 2.522 ± 0.059
0.0GlyXaa: 0.0 ± 0.0
His
2.375HisAla: 2.375 ± 0.059
0.218HisCys: 0.218 ± 0.019
1.066HisAsp: 1.066 ± 0.045
1.153HisGlu: 1.153 ± 0.048
0.909HisPhe: 0.909 ± 0.039
1.944HisGly: 1.944 ± 0.055
0.564HisHis: 0.564 ± 0.033
0.867HisIle: 0.867 ± 0.034
0.658HisLys: 0.658 ± 0.029
1.997HisLeu: 1.997 ± 0.056
0.453HisMet: 0.453 ± 0.026
0.529HisAsn: 0.529 ± 0.029
1.372HisPro: 1.372 ± 0.049
0.543HisGln: 0.543 ± 0.035
1.31HisArg: 1.31 ± 0.049
0.98HisSer: 0.98 ± 0.035
0.894HisThr: 0.894 ± 0.037
1.652HisVal: 1.652 ± 0.049
0.305HisTrp: 0.305 ± 0.023
0.616HisTyr: 0.616 ± 0.034
0.0HisXaa: 0.0 ± 0.0
Ile
6.552IleAla: 6.552 ± 0.107
0.47IleCys: 0.47 ± 0.03
3.056IleAsp: 3.056 ± 0.08
3.139IleGlu: 3.139 ± 0.069
1.552IlePhe: 1.552 ± 0.05
4.45IleGly: 4.45 ± 0.093
0.918IleHis: 0.918 ± 0.042
1.614IleIle: 1.614 ± 0.057
1.639IleLys: 1.639 ± 0.055
4.25IleLeu: 4.25 ± 0.096
0.785IleMet: 0.785 ± 0.038
1.329IleAsn: 1.329 ± 0.048
2.34IlePro: 2.34 ± 0.067
1.244IleGln: 1.244 ± 0.053
2.981IleArg: 2.981 ± 0.072
2.524IleSer: 2.524 ± 0.071
2.362IleThr: 2.362 ± 0.072
4.028IleVal: 4.028 ± 0.082
0.497IleTrp: 0.497 ± 0.027
1.158IleTyr: 1.158 ± 0.047
0.0IleXaa: 0.0 ± 0.0
Lys
4.786LysAla: 4.786 ± 0.1
0.267LysCys: 0.267 ± 0.022
2.355LysAsp: 2.355 ± 0.066
2.475LysGlu: 2.475 ± 0.064
1.301LysPhe: 1.301 ± 0.045
3.409LysGly: 3.409 ± 0.081
0.864LysHis: 0.864 ± 0.037
2.16LysIle: 2.16 ± 0.064
2.637LysLys: 2.637 ± 0.079
4.724LysLeu: 4.724 ± 0.087
1.152LysMet: 1.152 ± 0.042
1.414LysAsn: 1.414 ± 0.048
2.74LysPro: 2.74 ± 0.07
1.612LysGln: 1.612 ± 0.057
3.216LysArg: 3.216 ± 0.078
2.259LysSer: 2.259 ± 0.061
2.271LysThr: 2.271 ± 0.06
3.266LysVal: 3.266 ± 0.075
0.542LysTrp: 0.542 ± 0.029
0.969LysTyr: 0.969 ± 0.041
0.0LysXaa: 0.0 ± 0.0
Leu
14.555LeuAla: 14.555 ± 0.203
0.955LeuCys: 0.955 ± 0.04
5.329LeuAsp: 5.329 ± 0.086
6.336LeuGlu: 6.336 ± 0.128
3.791LeuPhe: 3.791 ± 0.083
8.502LeuGly: 8.502 ± 0.135
1.998LeuHis: 1.998 ± 0.051
4.384LeuIle: 4.384 ± 0.104
4.714LeuLys: 4.714 ± 0.094
10.577LeuLeu: 10.577 ± 0.161
2.105LeuMet: 2.105 ± 0.059
2.53LeuAsn: 2.53 ± 0.063
5.418LeuPro: 5.418 ± 0.102
3.213LeuGln: 3.213 ± 0.076
7.402LeuArg: 7.402 ± 0.131
5.661LeuSer: 5.661 ± 0.105
4.724LeuThr: 4.724 ± 0.108
7.908LeuVal: 7.908 ± 0.124
1.361LeuTrp: 1.361 ± 0.049
2.281LeuTyr: 2.281 ± 0.059
0.0LeuXaa: 0.0 ± 0.0
Met
2.749MetAla: 2.749 ± 0.065
0.186MetCys: 0.186 ± 0.019
1.09MetAsp: 1.09 ± 0.042
1.142MetGlu: 1.142 ± 0.041
0.721MetPhe: 0.721 ± 0.037
1.817MetGly: 1.817 ± 0.056
0.534MetHis: 0.534 ± 0.026
0.988MetIle: 0.988 ± 0.043
1.515MetLys: 1.515 ± 0.049
2.606MetLeu: 2.606 ± 0.059
0.562MetMet: 0.562 ± 0.028
0.764MetAsn: 0.764 ± 0.029
1.417MetPro: 1.417 ± 0.053
0.939MetGln: 0.939 ± 0.038
1.935MetArg: 1.935 ± 0.042
1.391MetSer: 1.391 ± 0.046
1.358MetThr: 1.358 ± 0.052
1.661MetVal: 1.661 ± 0.055
0.226MetTrp: 0.226 ± 0.019
0.419MetTyr: 0.419 ± 0.027
0.0MetXaa: 0.0 ± 0.0
Asn
2.962AsnAla: 2.962 ± 0.077
0.283AsnCys: 0.283 ± 0.022
1.223AsnAsp: 1.223 ± 0.049
1.342AsnGlu: 1.342 ± 0.046
1.012AsnPhe: 1.012 ± 0.044
2.143AsnGly: 2.143 ± 0.069
0.467AsnHis: 0.467 ± 0.031
1.19AsnIle: 1.19 ± 0.047
0.902AsnLys: 0.902 ± 0.038
2.74AsnLeu: 2.74 ± 0.066
0.553AsnMet: 0.553 ± 0.029
0.712AsnAsn: 0.712 ± 0.041
2.019AsnPro: 2.019 ± 0.062
0.747AsnGln: 0.747 ± 0.031
1.863AsnArg: 1.863 ± 0.056
1.228AsnSer: 1.228 ± 0.055
1.301AsnThr: 1.301 ± 0.056
2.146AsnVal: 2.146 ± 0.065
0.432AsnTrp: 0.432 ± 0.026
0.708AsnTyr: 0.708 ± 0.03
0.0AsnXaa: 0.0 ± 0.0
Pro
6.263ProAla: 6.263 ± 0.135
0.338ProCys: 0.338 ± 0.022
3.026ProAsp: 3.026 ± 0.078
3.736ProGlu: 3.736 ± 0.079
2.055ProPhe: 2.055 ± 0.057
4.67ProGly: 4.67 ± 0.096
1.134ProHis: 1.134 ± 0.046
2.122ProIle: 2.122 ± 0.054
2.257ProLys: 2.257 ± 0.068
4.697ProLeu: 4.697 ± 0.086
1.236ProMet: 1.236 ± 0.046
1.315ProAsn: 1.315 ± 0.054
2.579ProPro: 2.579 ± 0.084
1.903ProGln: 1.903 ± 0.052
3.226ProArg: 3.226 ± 0.077
2.532ProSer: 2.532 ± 0.072
2.201ProThr: 2.201 ± 0.063
4.195ProVal: 4.195 ± 0.077
0.754ProTrp: 0.754 ± 0.034
1.342ProTyr: 1.342 ± 0.049
0.0ProXaa: 0.0 ± 0.0
Gln
3.999GlnAla: 3.999 ± 0.1
0.26GlnCys: 0.26 ± 0.02
1.369GlnAsp: 1.369 ± 0.048
1.625GlnGlu: 1.625 ± 0.052
1.199GlnPhe: 1.199 ± 0.042
2.517GlnGly: 2.517 ± 0.066
0.726GlnHis: 0.726 ± 0.033
1.719GlnIle: 1.719 ± 0.051
1.655GlnLys: 1.655 ± 0.05
3.159GlnLeu: 3.159 ± 0.084
0.899GlnMet: 0.899 ± 0.039
0.786GlnAsn: 0.786 ± 0.037
1.793GlnPro: 1.793 ± 0.058
1.31GlnGln: 1.31 ± 0.056
2.614GlnArg: 2.614 ± 0.08
1.587GlnSer: 1.587 ± 0.057
1.509GlnThr: 1.509 ± 0.055
2.497GlnVal: 2.497 ± 0.066
0.419GlnTrp: 0.419 ± 0.024
0.837GlnTyr: 0.837 ± 0.036
0.0GlnXaa: 0.0 ± 0.0
Arg
8.134ArgAla: 8.134 ± 0.148
0.594ArgCys: 0.594 ± 0.031
3.807ArgAsp: 3.807 ± 0.078
4.822ArgGlu: 4.822 ± 0.111
3.05ArgPhe: 3.05 ± 0.078
5.232ArgGly: 5.232 ± 0.107
1.668ArgHis: 1.668 ± 0.061
3.95ArgIle: 3.95 ± 0.077
2.95ArgLys: 2.95 ± 0.073
7.551ArgLeu: 7.551 ± 0.131
1.865ArgMet: 1.865 ± 0.053
1.917ArgAsn: 1.917 ± 0.059
3.224ArgPro: 3.224 ± 0.082
2.287ArgGln: 2.287 ± 0.08
5.44ArgArg: 5.44 ± 0.109
3.626ArgSer: 3.626 ± 0.084
3.164ArgThr: 3.164 ± 0.066
5.454ArgVal: 5.454 ± 0.112
1.142ArgTrp: 1.142 ± 0.046
2.138ArgTyr: 2.138 ± 0.06
0.0ArgXaa: 0.0 ± 0.0
Ser
5.753SerAla: 5.753 ± 0.101
0.492SerCys: 0.492 ± 0.026
2.328SerAsp: 2.328 ± 0.069
2.705SerGlu: 2.705 ± 0.072
2.351SerPhe: 2.351 ± 0.056
5.434SerGly: 5.434 ± 0.114
1.056SerHis: 1.056 ± 0.038
2.51SerIle: 2.51 ± 0.084
2.004SerLys: 2.004 ± 0.052
5.529SerLeu: 5.529 ± 0.095
1.355SerMet: 1.355 ± 0.046
1.288SerAsn: 1.288 ± 0.051
2.822SerPro: 2.822 ± 0.07
1.642SerGln: 1.642 ± 0.053
3.626SerArg: 3.626 ± 0.079
2.903SerSer: 2.903 ± 0.075
2.541SerThr: 2.541 ± 0.078
3.677SerVal: 3.677 ± 0.082
0.759SerTrp: 0.759 ± 0.036
1.356SerTyr: 1.356 ± 0.044
0.0SerXaa: 0.0 ± 0.0
Thr
5.253ThrAla: 5.253 ± 0.096
0.367ThrCys: 0.367 ± 0.026
2.222ThrAsp: 2.222 ± 0.058
2.27ThrGlu: 2.27 ± 0.064
1.852ThrPhe: 1.852 ± 0.059
4.287ThrGly: 4.287 ± 0.102
1.04ThrHis: 1.04 ± 0.042
2.332ThrIle: 2.332 ± 0.066
1.609ThrLys: 1.609 ± 0.056
5.801ThrLeu: 5.801 ± 0.12
1.121ThrMet: 1.121 ± 0.042
1.229ThrAsn: 1.229 ± 0.058
3.21ThrPro: 3.21 ± 0.101
1.469ThrGln: 1.469 ± 0.067
3.253ThrArg: 3.253 ± 0.069
2.419ThrSer: 2.419 ± 0.084
2.462ThrThr: 2.462 ± 0.102
3.915ThrVal: 3.915 ± 0.095
0.669ThrTrp: 0.669 ± 0.034
1.147ThrTyr: 1.147 ± 0.042
0.0ThrXaa: 0.0 ± 0.0
Val
9.335ValAla: 9.335 ± 0.129
0.759ValCys: 0.759 ± 0.043
4.092ValAsp: 4.092 ± 0.076
4.614ValGlu: 4.614 ± 0.098
2.924ValPhe: 2.924 ± 0.066
5.499ValGly: 5.499 ± 0.104
1.528ValHis: 1.528 ± 0.056
3.812ValIle: 3.812 ± 0.08
3.267ValLys: 3.267 ± 0.078
8.196ValLeu: 8.196 ± 0.131
1.874ValMet: 1.874 ± 0.059
2.16ValAsn: 2.16 ± 0.062
4.136ValPro: 4.136 ± 0.092
2.409ValGln: 2.409 ± 0.065
5.376ValArg: 5.376 ± 0.099
4.192ValSer: 4.192 ± 0.089
4.06ValThr: 4.06 ± 0.1
6.717ValVal: 6.717 ± 0.127
0.978ValTrp: 0.978 ± 0.036
1.754ValTyr: 1.754 ± 0.059
0.0ValXaa: 0.0 ± 0.0
Trp
1.229TrpAla: 1.229 ± 0.048
0.152TrpCys: 0.152 ± 0.015
0.651TrpAsp: 0.651 ± 0.033
0.615TrpGlu: 0.615 ± 0.029
0.519TrpPhe: 0.519 ± 0.034
0.996TrpGly: 0.996 ± 0.044
0.364TrpHis: 0.364 ± 0.024
0.723TrpIle: 0.723 ± 0.033
0.699TrpLys: 0.699 ± 0.035
1.854TrpLeu: 1.854 ± 0.062
0.376TrpMet: 0.376 ± 0.029
0.51TrpAsn: 0.51 ± 0.031
0.605TrpPro: 0.605 ± 0.032
0.65TrpGln: 0.65 ± 0.031
1.226TrpArg: 1.226 ± 0.049
0.761TrpSer: 0.761 ± 0.039
0.678TrpThr: 0.678 ± 0.04
0.931TrpVal: 0.931 ± 0.038
0.227TrpTrp: 0.227 ± 0.019
0.378TrpTyr: 0.378 ± 0.025
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.83TyrAla: 2.83 ± 0.07
0.3TyrCys: 0.3 ± 0.022
1.377TyrAsp: 1.377 ± 0.047
1.375TyrGlu: 1.375 ± 0.046
1.053TyrPhe: 1.053 ± 0.04
2.317TyrGly: 2.317 ± 0.064
0.502TyrHis: 0.502 ± 0.031
0.902TyrIle: 0.902 ± 0.034
0.932TyrLys: 0.932 ± 0.035
2.532TyrLeu: 2.532 ± 0.064
0.492TyrMet: 0.492 ± 0.029
0.627TyrAsn: 0.627 ± 0.036
1.321TyrPro: 1.321 ± 0.044
0.821TyrGln: 0.821 ± 0.036
1.984TyrArg: 1.984 ± 0.051
1.336TyrSer: 1.336 ± 0.051
1.24TyrThr: 1.24 ± 0.045
2.02TyrVal: 2.02 ± 0.056
0.405TyrTrp: 0.405 ± 0.025
0.669TyrTyr: 0.669 ± 0.039
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2067 proteins (629598 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski