Dyadobacter koreensis
Average proteome isoelectric point is 6.74
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5986 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1H6XL40|A0A1H6XL40_9BACT 50S ribosomal protein L20 OS=Dyadobacter koreensis OX=408657 GN=rplT PE=3 SV=1
MM1 pKa = 8.24 DD2 pKa = 4.91 SFLEE6 pKa = 4.39 KK7 pKa = 10.6 YY8 pKa = 10.03 SPKK11 pKa = 10.0 IEE13 pKa = 4.36 NEE15 pKa = 3.85 PASLQDD21 pKa = 3.28 IAQLEE26 pKa = 4.37 AEE28 pKa = 4.46 LGIRR32 pKa = 11.84 LPSDD36 pKa = 3.96 FSTFLRR42 pKa = 11.84 NVNGFEE48 pKa = 4.05 GFIGNSYY55 pKa = 10.97 AIFAPLQAIYY65 pKa = 10.98 QMTLDD70 pKa = 4.16 YY71 pKa = 10.89 CAEE74 pKa = 4.64 LYY76 pKa = 9.82 PWAVFIGSNGGLEE89 pKa = 3.96 MFIIDD94 pKa = 4.3 TRR96 pKa = 11.84 SSPYY100 pKa = 10.45 QFGLLPFIADD110 pKa = 3.59 DD111 pKa = 3.79 SDD113 pKa = 4.05 FVGFGQSLEE122 pKa = 4.15 QFLEE126 pKa = 4.05 MLANLL131 pKa = 4.27
Molecular weight: 14.7 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.752
IPC2_protein 3.821
IPC_protein 3.732
Toseland 3.554
ProMoST 3.897
Dawson 3.719
Bjellqvist 3.872
Wikipedia 3.63
Rodwell 3.579
Grimsley 3.465
Solomon 3.694
Lehninger 3.643
Nozaki 3.846
DTASelect 3.999
Thurlkill 3.605
EMBOSS 3.643
Sillero 3.859
Patrickios 0.769
IPC_peptide 3.694
IPC2_peptide 3.834
IPC2.peptide.svr19 3.763
Protein with the highest isoelectric point:
>tr|A0A1H6R6A5|A0A1H6R6A5_9BACT Uncharacterized protein OS=Dyadobacter koreensis OX=408657 GN=SAMN04487995_1058 PE=4 SV=1
MM1 pKa = 7.45 AASKK5 pKa = 10.5 KK6 pKa = 9.99 KK7 pKa = 10.72 RR8 pKa = 11.84 NTKK11 pKa = 9.79 KK12 pKa = 10.68 NKK14 pKa = 10.21 LGVKK18 pKa = 10.16 NSLVNNINARR28 pKa = 11.84 KK29 pKa = 9.73 KK30 pKa = 10.56 KK31 pKa = 9.01 GTSRR35 pKa = 11.84 PKK37 pKa = 10.24 KK38 pKa = 9.91 KK39 pKa = 9.29 KK40 pKa = 7.84 TVSKK44 pKa = 10.67 KK45 pKa = 10.71 SYY47 pKa = 10.36 TKK49 pKa = 9.61 MQNNWGKK56 pKa = 10.38 KK57 pKa = 8.82 RR58 pKa = 11.84 STKK61 pKa = 8.93 NRR63 pKa = 11.84 KK64 pKa = 9.21 RR65 pKa = 11.84 KK66 pKa = 7.53 TKK68 pKa = 10.2 RR69 pKa = 11.84 KK70 pKa = 8.93 SRR72 pKa = 11.84 KK73 pKa = 8.85
Molecular weight: 8.57 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.366
IPC2_protein 11.096
IPC_protein 12.325
Toseland 12.544
ProMoST 12.983
Dawson 12.544
Bjellqvist 12.501
Wikipedia 12.983
Rodwell 12.691
Grimsley 12.574
Solomon 12.998
Lehninger 12.91
Nozaki 12.53
DTASelect 12.501
Thurlkill 12.53
EMBOSS 13.027
Sillero 12.53
Patrickios 12.398
IPC_peptide 12.998
IPC2_peptide 11.974
IPC2.peptide.svr19 8.849
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5986
0
5986
2116298
38
2392
353.5
39.67
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.14 ± 0.033
0.718 ± 0.01
5.336 ± 0.023
5.976 ± 0.036
5.097 ± 0.022
6.921 ± 0.032
1.788 ± 0.017
7.14 ± 0.026
6.863 ± 0.026
9.513 ± 0.032
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.253 ± 0.015
5.362 ± 0.028
3.894 ± 0.019
3.665 ± 0.017
4.215 ± 0.019
6.848 ± 0.026
5.68 ± 0.027
6.396 ± 0.021
1.254 ± 0.013
3.944 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here