Rotavirus F chicken/03V0568/DEU/2003

Taxonomy: Viruses; Riboviria; Orthornavirae; Duplornaviricota; Resentoviricetes; Reovirales; Reoviridae; Sedoreovirinae; Rotavirus; Rotavirus F

Average proteome isoelectric point is 6.32

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 11 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|M4H2P5|M4H2P5_9REOV NSP4 OS=Rotavirus F chicken/03V0568/DEU/2003 OX=994994 PE=4 SV=1
MM1 pKa = 8.03ASRR4 pKa = 11.84FWVGIINDD12 pKa = 3.89WQNVQARR19 pKa = 11.84QQTIAQTSNKK29 pKa = 9.52KK30 pKa = 10.19FEE32 pKa = 4.39TYY34 pKa = 10.25IDD36 pKa = 3.6TSNVGTAQLPPSNFSNDD53 pKa = 3.27NMSQPSEE60 pKa = 4.25DD61 pKa = 3.48VSSSLWYY68 pKa = 10.56GPNKK72 pKa = 9.63PQILTNAEE80 pKa = 4.0YY81 pKa = 10.63QQVKK85 pKa = 8.39PAKK88 pKa = 10.17LFFKK92 pKa = 10.92FEE94 pKa = 4.2TPKK97 pKa = 10.55QYY99 pKa = 11.46LMWQVDD105 pKa = 3.51NYY107 pKa = 11.62AAGFYY112 pKa = 10.63KK113 pKa = 10.59KK114 pKa = 10.33GDD116 pKa = 3.59NFGTEE121 pKa = 4.19VIKK124 pKa = 10.74DD125 pKa = 3.42DD126 pKa = 5.0SIIIVYY132 pKa = 10.46DD133 pKa = 3.14KK134 pKa = 11.07TSNQKK139 pKa = 10.46LFTSTIINSIVFSISEE155 pKa = 4.0MTSNQLEE162 pKa = 4.17LTAYY166 pKa = 8.58QGNMSRR172 pKa = 11.84SIANITDD179 pKa = 3.15QNFNLDD185 pKa = 3.12IRR187 pKa = 11.84QDD189 pKa = 3.92YY190 pKa = 9.45YY191 pKa = 11.83SCASTLFPTLYY202 pKa = 9.84KK203 pKa = 10.86NEE205 pKa = 3.67MHH207 pKa = 6.79YY208 pKa = 10.88VIEE211 pKa = 4.47RR212 pKa = 11.84NAKK215 pKa = 8.95VLSKK219 pKa = 10.9ALTEE223 pKa = 4.11EE224 pKa = 4.59TVVSQAGLWKK234 pKa = 9.56EE235 pKa = 3.72VRR237 pKa = 11.84YY238 pKa = 9.47AADD241 pKa = 2.91ITLRR245 pKa = 11.84FYY247 pKa = 10.74FQSEE251 pKa = 4.84AIRR254 pKa = 11.84KK255 pKa = 9.51GGFGYY260 pKa = 10.47QFSTSNFKK268 pKa = 10.23PWTSVYY274 pKa = 10.67SYY276 pKa = 11.04TRR278 pKa = 11.84DD279 pKa = 3.62NEE281 pKa = 4.09NVTAHH286 pKa = 5.12VTVTLNGLSVYY297 pKa = 7.34SQRR300 pKa = 11.84GAVPTDD306 pKa = 3.51FAIDD310 pKa = 3.32RR311 pKa = 11.84MEE313 pKa = 5.66CITNDD318 pKa = 2.74TYY320 pKa = 11.09MYY322 pKa = 10.12IDD324 pKa = 3.63YY325 pKa = 9.92WDD327 pKa = 3.59TSNLFRR333 pKa = 11.84NMVYY337 pKa = 10.33VRR339 pKa = 11.84DD340 pKa = 3.95LKK342 pKa = 11.25VEE344 pKa = 3.85QSSIRR349 pKa = 11.84IGFWPYY355 pKa = 9.83MRR357 pKa = 11.84SLTLPVGSITPYY369 pKa = 9.91YY370 pKa = 9.81PFGITPNGVINADD383 pKa = 4.94LICEE387 pKa = 5.32GITLSTQFTDD397 pKa = 3.39AASMNSLRR405 pKa = 11.84FIFKK409 pKa = 9.98FKK411 pKa = 11.13NFDD414 pKa = 3.76PPNGNITRR422 pKa = 11.84MKK424 pKa = 8.89VTGVPAAYY432 pKa = 8.5MQYY435 pKa = 10.63AQVGGNPGNPGDD447 pKa = 3.77PFNYY451 pKa = 9.43NVRR454 pKa = 11.84FSMISLIPANDD465 pKa = 3.72EE466 pKa = 4.17YY467 pKa = 10.32KK468 pKa = 10.41TPIAASVTVRR478 pKa = 11.84QDD480 pKa = 3.34LDD482 pKa = 3.32IKK484 pKa = 10.81LSQLRR489 pKa = 11.84QEE491 pKa = 4.55FNQVAEE497 pKa = 4.23NLAVSQAIGLATMPFDD513 pKa = 3.87MLSMFSGTFDD523 pKa = 3.26VVQNIGDD530 pKa = 3.79FTSTLFSKK538 pKa = 10.8FSKK541 pKa = 7.86TTTGVQLLKK550 pKa = 8.92YY551 pKa = 8.69TKK553 pKa = 9.54YY554 pKa = 9.06TDD556 pKa = 3.55EE557 pKa = 4.65LVDD560 pKa = 3.69VATSTDD566 pKa = 3.5DD567 pKa = 3.52VLEE570 pKa = 4.26VATIANKK577 pKa = 10.23AKK579 pKa = 10.18IMKK582 pKa = 9.88SVEE585 pKa = 4.1TMTDD589 pKa = 2.88SLDD592 pKa = 3.78FEE594 pKa = 5.81DD595 pKa = 5.44ISTAVLNKK603 pKa = 10.07AMRR606 pKa = 11.84EE607 pKa = 3.85IGTQTDD613 pKa = 4.05YY614 pKa = 11.94NSIAQIAAEE623 pKa = 4.8SIDD626 pKa = 4.19MIPTKK631 pKa = 10.35AYY633 pKa = 10.14RR634 pKa = 11.84VIDD637 pKa = 3.78NASDD641 pKa = 3.4TVYY644 pKa = 10.65EE645 pKa = 4.1VSKK648 pKa = 10.61DD649 pKa = 3.35ANIAYY654 pKa = 9.64KK655 pKa = 9.55LTDD658 pKa = 3.9LSQIQFDD665 pKa = 3.19IDD667 pKa = 3.55KK668 pKa = 10.21FKK670 pKa = 11.01KK671 pKa = 10.27LSSEE675 pKa = 4.33SPVLSAIIDD684 pKa = 4.12FKK686 pKa = 9.6TIKK689 pKa = 9.92MMNQANGKK697 pKa = 9.18LDD699 pKa = 5.59RR700 pKa = 11.84ITLDD704 pKa = 3.45NLLASNPQILKK715 pKa = 9.82TMIAQNNPIIVRR727 pKa = 11.84RR728 pKa = 11.84VQEE731 pKa = 5.41LIDD734 pKa = 3.46QCRR737 pKa = 11.84LL738 pKa = 3.16

Molecular weight:
83.33 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|M4H1X6|M4H1X6_9REOV Inner capsid protein VP2 OS=Rotavirus F chicken/03V0568/DEU/2003 OX=994994 PE=3 SV=1
MM1 pKa = 6.9GTYY4 pKa = 10.18DD5 pKa = 4.56ALLEE9 pKa = 4.22EE10 pKa = 5.27YY11 pKa = 10.28IHH13 pKa = 6.67FLYY16 pKa = 10.85SDD18 pKa = 4.56DD19 pKa = 4.07SLSVIPIYY27 pKa = 9.58YY28 pKa = 8.97TSNVDD33 pKa = 2.95IHH35 pKa = 7.08QEE37 pKa = 3.43LSKK40 pKa = 11.23YY41 pKa = 8.19HH42 pKa = 6.43TEE44 pKa = 4.11LIEE47 pKa = 3.97YY48 pKa = 9.9NKK50 pKa = 10.26IKK52 pKa = 10.83ARR54 pKa = 11.84TKK56 pKa = 10.06KK57 pKa = 9.88HH58 pKa = 6.45DD59 pKa = 3.78FNKK62 pKa = 10.51LEE64 pKa = 4.67SIHH67 pKa = 6.62AKK69 pKa = 9.95YY70 pKa = 10.56NEE72 pKa = 4.74SISKK76 pKa = 9.81SRR78 pKa = 11.84RR79 pKa = 11.84LSIVVYY85 pKa = 10.26AYY87 pKa = 9.99GKK89 pKa = 9.8HH90 pKa = 6.18DD91 pKa = 3.63AVEE94 pKa = 4.11RR95 pKa = 11.84KK96 pKa = 8.65IVKK99 pKa = 9.88YY100 pKa = 9.16ATKK103 pKa = 10.34KK104 pKa = 10.35PLIADD109 pKa = 4.37LTANEE114 pKa = 4.9LDD116 pKa = 3.81YY117 pKa = 11.68EE118 pKa = 4.42NNKK121 pKa = 8.14LTKK124 pKa = 10.14DD125 pKa = 3.38LYY127 pKa = 10.86DD128 pKa = 3.65EE129 pKa = 4.93KK130 pKa = 11.31SYY132 pKa = 11.32TDD134 pKa = 3.81YY135 pKa = 11.84LMDD138 pKa = 4.51PAISTSLTSNLNACMFTIEE157 pKa = 4.43TEE159 pKa = 4.3TNEE162 pKa = 4.27KK163 pKa = 8.94QRR165 pKa = 11.84KK166 pKa = 8.57RR167 pKa = 11.84YY168 pKa = 9.57YY169 pKa = 10.33EE170 pKa = 4.07LFTIVASTLHH180 pKa = 6.54HH181 pKa = 6.12YY182 pKa = 8.84GIPRR186 pKa = 11.84HH187 pKa = 5.66DD188 pKa = 3.4SKK190 pKa = 11.67YY191 pKa = 10.11RR192 pKa = 11.84YY193 pKa = 8.06TYY195 pKa = 10.16EE196 pKa = 3.87QMRR199 pKa = 11.84DD200 pKa = 2.95KK201 pKa = 10.59PYY203 pKa = 11.12YY204 pKa = 8.81IVPWANSAIEE214 pKa = 4.0MLMSVNNHH222 pKa = 6.62DD223 pKa = 4.31DD224 pKa = 3.66FVIARR229 pKa = 11.84EE230 pKa = 4.11LITYY234 pKa = 8.73SFSNRR239 pKa = 11.84STLAKK244 pKa = 9.77IVSSPMSIMTALLDD258 pKa = 3.61INGTFITDD266 pKa = 3.17EE267 pKa = 4.17SLQLQYY273 pKa = 10.9SSKK276 pKa = 9.86MIKK279 pKa = 10.55ALVTDD284 pKa = 3.89EE285 pKa = 4.29AKK287 pKa = 10.68KK288 pKa = 10.36EE289 pKa = 3.93LEE291 pKa = 3.96EE292 pKa = 4.2MLQKK296 pKa = 10.42MIDD299 pKa = 3.8LGLVDD304 pKa = 4.96IPNILLSWIEE314 pKa = 3.98NPRR317 pKa = 11.84LDD319 pKa = 3.65TFHH322 pKa = 7.26RR323 pKa = 11.84VANVYY328 pKa = 9.54SWSFHH333 pKa = 4.96VGYY336 pKa = 10.17RR337 pKa = 11.84KK338 pKa = 9.6QLMQDD343 pKa = 3.15AVQDD347 pKa = 3.92QLSVEE352 pKa = 4.09YY353 pKa = 10.73SEE355 pKa = 5.96NVDD358 pKa = 2.62KK359 pKa = 11.23TMYY362 pKa = 10.49DD363 pKa = 2.86EE364 pKa = 4.43YY365 pKa = 11.51YY366 pKa = 11.0NYY368 pKa = 10.03IKK370 pKa = 10.46EE371 pKa = 4.17DD372 pKa = 4.11YY373 pKa = 10.71IRR375 pKa = 11.84MLKK378 pKa = 10.58DD379 pKa = 3.54DD380 pKa = 4.71VIKK383 pKa = 10.25STTILKK389 pKa = 8.97QHH391 pKa = 6.22EE392 pKa = 4.33LAGLLSMSSASNGKK406 pKa = 9.99KK407 pKa = 8.97MEE409 pKa = 4.14IKK411 pKa = 10.46FGSKK415 pKa = 10.3KK416 pKa = 10.72LFTTRR421 pKa = 11.84KK422 pKa = 8.47NMHH425 pKa = 5.69VMSDD429 pKa = 3.56VSSGNYY435 pKa = 9.16NSSIVPPVDD444 pKa = 2.91ATSPIPLGRR453 pKa = 11.84RR454 pKa = 11.84DD455 pKa = 3.43VPGRR459 pKa = 11.84RR460 pKa = 11.84TRR462 pKa = 11.84VIFILPYY469 pKa = 10.05EE470 pKa = 4.26YY471 pKa = 10.73FLAQHH476 pKa = 7.04AIVEE480 pKa = 4.38LLLKK484 pKa = 9.29QAKK487 pKa = 7.48HH488 pKa = 4.67TRR490 pKa = 11.84EE491 pKa = 3.38FSEE494 pKa = 5.56FYY496 pKa = 11.03SSANQLLSYY505 pKa = 10.05GDD507 pKa = 3.56TMRR510 pKa = 11.84EE511 pKa = 4.2LNPSTIIIYY520 pKa = 10.06TDD522 pKa = 3.15VSQWDD527 pKa = 4.35GSQHH531 pKa = 4.36NTQPFRR537 pKa = 11.84KK538 pKa = 9.54AIMAAMQDD546 pKa = 3.69LQAYY550 pKa = 5.96TTDD553 pKa = 3.62QNVEE557 pKa = 3.84RR558 pKa = 11.84ALNKK562 pKa = 10.15YY563 pKa = 10.47YY564 pKa = 8.48EE565 pKa = 4.36TQRR568 pKa = 11.84QLMNSYY574 pKa = 8.42VTIDD578 pKa = 4.18DD579 pKa = 5.23KK580 pKa = 11.09IIQYY584 pKa = 10.46GAVASGEE591 pKa = 4.23KK592 pKa = 7.29QTKK595 pKa = 8.23IANSIANLALIKK607 pKa = 9.89TVLNKK612 pKa = 10.31LSAKK616 pKa = 8.5YY617 pKa = 8.72TFNIKK622 pKa = 10.5LIRR625 pKa = 11.84VDD627 pKa = 3.99GDD629 pKa = 3.57DD630 pKa = 3.82NYY632 pKa = 11.49TVIEE636 pKa = 4.26SRR638 pKa = 11.84SQVTQEE644 pKa = 4.25LLSNLSKK651 pKa = 10.71DD652 pKa = 3.53IKK654 pKa = 10.23DD655 pKa = 3.67TYY657 pKa = 11.9ALMNVKK663 pKa = 9.89VKK665 pKa = 10.91ALVSTTGLEE674 pKa = 3.79MAKK677 pKa = 10.03RR678 pKa = 11.84YY679 pKa = 9.17IAGGRR684 pKa = 11.84IYY686 pKa = 10.48FRR688 pKa = 11.84AGINILNNEE697 pKa = 4.19KK698 pKa = 10.52RR699 pKa = 11.84NQQTAWDD706 pKa = 3.89QAAILYY712 pKa = 10.21ASYY715 pKa = 9.67TVNKK719 pKa = 10.03LRR721 pKa = 11.84GFITNRR727 pKa = 11.84TFILTKK733 pKa = 9.8IMQMTSVKK741 pKa = 10.04ISGVLRR747 pKa = 11.84IFPASDD753 pKa = 3.1VLTLNSTYY761 pKa = 10.98KK762 pKa = 10.7VFDD765 pKa = 3.74EE766 pKa = 3.66VDD768 pKa = 3.28YY769 pKa = 11.33AVNYY773 pKa = 10.21KK774 pKa = 9.93VDD776 pKa = 3.95ADD778 pKa = 3.83SLMLQKK784 pKa = 11.04LLIKK788 pKa = 10.56VSQSRR793 pKa = 11.84SKK795 pKa = 11.09VADD798 pKa = 4.66EE799 pKa = 4.24ISKK802 pKa = 9.87SHH804 pKa = 6.45IFSNYY809 pKa = 7.11VKK811 pKa = 10.6YY812 pKa = 10.73ISTMLLKK819 pKa = 10.34IPNKK823 pKa = 9.95TIEE826 pKa = 4.03QGLAKK831 pKa = 9.86TEE833 pKa = 4.0KK834 pKa = 10.48AKK836 pKa = 11.0LNSYY840 pKa = 9.99PPISLEE846 pKa = 4.12KK847 pKa = 10.56RR848 pKa = 11.84KK849 pKa = 7.8TQLDD853 pKa = 3.95TLIKK857 pKa = 8.58TLQYY861 pKa = 10.13PIIYY865 pKa = 10.12VGGKK869 pKa = 5.13TTLNDD874 pKa = 3.19VVSVVDD880 pKa = 3.52QYY882 pKa = 12.22VEE884 pKa = 4.12YY885 pKa = 10.94KK886 pKa = 10.88SIDD889 pKa = 3.45PEE891 pKa = 4.03KK892 pKa = 10.46KK893 pKa = 8.7YY894 pKa = 11.0RR895 pKa = 11.84NYY897 pKa = 11.16SPMLPTDD904 pKa = 3.07ISEE907 pKa = 4.48VITVIGSRR915 pKa = 11.84LSSYY919 pKa = 10.1EE920 pKa = 3.68DD921 pKa = 3.37HH922 pKa = 6.96AGKK925 pKa = 10.2SAIAKK930 pKa = 9.41LISTYY935 pKa = 10.44SVYY938 pKa = 10.48RR939 pKa = 11.84PSIEE943 pKa = 3.78EE944 pKa = 4.09LYY946 pKa = 10.93AIITKK951 pKa = 10.22RR952 pKa = 11.84EE953 pKa = 3.95NEE955 pKa = 3.77IKK957 pKa = 10.5LYY959 pKa = 9.26MLSLSVPLQDD969 pKa = 2.46VDD971 pKa = 5.3AYY973 pKa = 9.97IASQTYY979 pKa = 9.99KK980 pKa = 9.84RR981 pKa = 11.84DD982 pKa = 3.05KK983 pKa = 11.01YY984 pKa = 11.07LILQSYY990 pKa = 10.18VYY992 pKa = 10.86DD993 pKa = 4.12LLSINYY999 pKa = 8.58GAYY1002 pKa = 9.93QLFDD1006 pKa = 3.9FEE1008 pKa = 6.1SEE1010 pKa = 4.25FFSKK1014 pKa = 10.89YY1015 pKa = 5.71ITYY1018 pKa = 10.59VAMSKK1023 pKa = 10.32VPSAEE1028 pKa = 4.03FLVGTYY1034 pKa = 10.41VKK1036 pKa = 10.68LKK1038 pKa = 10.25IINRR1042 pKa = 11.84YY1043 pKa = 5.86LTEE1046 pKa = 4.31RR1047 pKa = 11.84KK1048 pKa = 8.79WYY1050 pKa = 9.11SANITIPKK1058 pKa = 8.8HH1059 pKa = 5.85EE1060 pKa = 4.11YY1061 pKa = 9.43MKK1063 pKa = 10.51INKK1066 pKa = 7.43LTWEE1070 pKa = 4.15ISSVRR1075 pKa = 11.84SPYY1078 pKa = 9.55TSANFFQEE1086 pKa = 3.91

Molecular weight:
125.38 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

11

0

11

5735

169

1086

521.4

59.89

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.242 ± 0.352

1.255 ± 0.399

5.754 ± 0.286

5.841 ± 0.633

3.854 ± 0.367

3.4 ± 0.321

1.779 ± 0.274

7.463 ± 0.366

6.556 ± 0.603

8.596 ± 0.527

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.173 ± 0.238

6.207 ± 0.463

2.964 ± 0.294

4.725 ± 0.388

4.917 ± 0.364

7.393 ± 0.597

7.027 ± 0.351

6.643 ± 0.368

0.837 ± 0.101

5.371 ± 0.518

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski