Rotavirus F chicken/03V0568/DEU/2003
Average proteome isoelectric point is 6.32
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 11 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|M4H2P5|M4H2P5_9REOV NSP4 OS=Rotavirus F chicken/03V0568/DEU/2003 OX=994994 PE=4 SV=1
MM1 pKa = 8.03 ASRR4 pKa = 11.84 FWVGIINDD12 pKa = 3.89 WQNVQARR19 pKa = 11.84 QQTIAQTSNKK29 pKa = 9.52 KK30 pKa = 10.19 FEE32 pKa = 4.39 TYY34 pKa = 10.25 IDD36 pKa = 3.6 TSNVGTAQLPPSNFSNDD53 pKa = 3.27 NMSQPSEE60 pKa = 4.25 DD61 pKa = 3.48 VSSSLWYY68 pKa = 10.56 GPNKK72 pKa = 9.63 PQILTNAEE80 pKa = 4.0 YY81 pKa = 10.63 QQVKK85 pKa = 8.39 PAKK88 pKa = 10.17 LFFKK92 pKa = 10.92 FEE94 pKa = 4.2 TPKK97 pKa = 10.55 QYY99 pKa = 11.46 LMWQVDD105 pKa = 3.51 NYY107 pKa = 11.62 AAGFYY112 pKa = 10.63 KK113 pKa = 10.59 KK114 pKa = 10.33 GDD116 pKa = 3.59 NFGTEE121 pKa = 4.19 VIKK124 pKa = 10.74 DD125 pKa = 3.42 DD126 pKa = 5.0 SIIIVYY132 pKa = 10.46 DD133 pKa = 3.14 KK134 pKa = 11.07 TSNQKK139 pKa = 10.46 LFTSTIINSIVFSISEE155 pKa = 4.0 MTSNQLEE162 pKa = 4.17 LTAYY166 pKa = 8.58 QGNMSRR172 pKa = 11.84 SIANITDD179 pKa = 3.15 QNFNLDD185 pKa = 3.12 IRR187 pKa = 11.84 QDD189 pKa = 3.92 YY190 pKa = 9.45 YY191 pKa = 11.83 SCASTLFPTLYY202 pKa = 9.84 KK203 pKa = 10.86 NEE205 pKa = 3.67 MHH207 pKa = 6.79 YY208 pKa = 10.88 VIEE211 pKa = 4.47 RR212 pKa = 11.84 NAKK215 pKa = 8.95 VLSKK219 pKa = 10.9 ALTEE223 pKa = 4.11 EE224 pKa = 4.59 TVVSQAGLWKK234 pKa = 9.56 EE235 pKa = 3.72 VRR237 pKa = 11.84 YY238 pKa = 9.47 AADD241 pKa = 2.91 ITLRR245 pKa = 11.84 FYY247 pKa = 10.74 FQSEE251 pKa = 4.84 AIRR254 pKa = 11.84 KK255 pKa = 9.51 GGFGYY260 pKa = 10.47 QFSTSNFKK268 pKa = 10.23 PWTSVYY274 pKa = 10.67 SYY276 pKa = 11.04 TRR278 pKa = 11.84 DD279 pKa = 3.62 NEE281 pKa = 4.09 NVTAHH286 pKa = 5.12 VTVTLNGLSVYY297 pKa = 7.34 SQRR300 pKa = 11.84 GAVPTDD306 pKa = 3.51 FAIDD310 pKa = 3.32 RR311 pKa = 11.84 MEE313 pKa = 5.66 CITNDD318 pKa = 2.74 TYY320 pKa = 11.09 MYY322 pKa = 10.12 IDD324 pKa = 3.63 YY325 pKa = 9.92 WDD327 pKa = 3.59 TSNLFRR333 pKa = 11.84 NMVYY337 pKa = 10.33 VRR339 pKa = 11.84 DD340 pKa = 3.95 LKK342 pKa = 11.25 VEE344 pKa = 3.85 QSSIRR349 pKa = 11.84 IGFWPYY355 pKa = 9.83 MRR357 pKa = 11.84 SLTLPVGSITPYY369 pKa = 9.91 YY370 pKa = 9.81 PFGITPNGVINADD383 pKa = 4.94 LICEE387 pKa = 5.32 GITLSTQFTDD397 pKa = 3.39 AASMNSLRR405 pKa = 11.84 FIFKK409 pKa = 9.98 FKK411 pKa = 11.13 NFDD414 pKa = 3.76 PPNGNITRR422 pKa = 11.84 MKK424 pKa = 8.89 VTGVPAAYY432 pKa = 8.5 MQYY435 pKa = 10.63 AQVGGNPGNPGDD447 pKa = 3.77 PFNYY451 pKa = 9.43 NVRR454 pKa = 11.84 FSMISLIPANDD465 pKa = 3.72 EE466 pKa = 4.17 YY467 pKa = 10.32 KK468 pKa = 10.41 TPIAASVTVRR478 pKa = 11.84 QDD480 pKa = 3.34 LDD482 pKa = 3.32 IKK484 pKa = 10.81 LSQLRR489 pKa = 11.84 QEE491 pKa = 4.55 FNQVAEE497 pKa = 4.23 NLAVSQAIGLATMPFDD513 pKa = 3.87 MLSMFSGTFDD523 pKa = 3.26 VVQNIGDD530 pKa = 3.79 FTSTLFSKK538 pKa = 10.8 FSKK541 pKa = 7.86 TTTGVQLLKK550 pKa = 8.92 YY551 pKa = 8.69 TKK553 pKa = 9.54 YY554 pKa = 9.06 TDD556 pKa = 3.55 EE557 pKa = 4.65 LVDD560 pKa = 3.69 VATSTDD566 pKa = 3.5 DD567 pKa = 3.52 VLEE570 pKa = 4.26 VATIANKK577 pKa = 10.23 AKK579 pKa = 10.18 IMKK582 pKa = 9.88 SVEE585 pKa = 4.1 TMTDD589 pKa = 2.88 SLDD592 pKa = 3.78 FEE594 pKa = 5.81 DD595 pKa = 5.44 ISTAVLNKK603 pKa = 10.07 AMRR606 pKa = 11.84 EE607 pKa = 3.85 IGTQTDD613 pKa = 4.05 YY614 pKa = 11.94 NSIAQIAAEE623 pKa = 4.8 SIDD626 pKa = 4.19 MIPTKK631 pKa = 10.35 AYY633 pKa = 10.14 RR634 pKa = 11.84 VIDD637 pKa = 3.78 NASDD641 pKa = 3.4 TVYY644 pKa = 10.65 EE645 pKa = 4.1 VSKK648 pKa = 10.61 DD649 pKa = 3.35 ANIAYY654 pKa = 9.64 KK655 pKa = 9.55 LTDD658 pKa = 3.9 LSQIQFDD665 pKa = 3.19 IDD667 pKa = 3.55 KK668 pKa = 10.21 FKK670 pKa = 11.01 KK671 pKa = 10.27 LSSEE675 pKa = 4.33 SPVLSAIIDD684 pKa = 4.12 FKK686 pKa = 9.6 TIKK689 pKa = 9.92 MMNQANGKK697 pKa = 9.18 LDD699 pKa = 5.59 RR700 pKa = 11.84 ITLDD704 pKa = 3.45 NLLASNPQILKK715 pKa = 9.82 TMIAQNNPIIVRR727 pKa = 11.84 RR728 pKa = 11.84 VQEE731 pKa = 5.41 LIDD734 pKa = 3.46 QCRR737 pKa = 11.84 LL738 pKa = 3.16
Molecular weight: 83.33 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.001
IPC2_protein 5.105
IPC_protein 5.054
Toseland 4.902
ProMoST 5.169
Dawson 5.016
Bjellqvist 5.156
Wikipedia 4.902
Rodwell 4.902
Grimsley 4.813
Solomon 5.016
Lehninger 4.965
Nozaki 5.118
DTASelect 5.334
Thurlkill 4.902
EMBOSS 4.927
Sillero 5.181
Patrickios 3.986
IPC_peptide 5.016
IPC2_peptide 5.169
IPC2.peptide.svr19 5.206
Protein with the highest isoelectric point:
>tr|M4H1X6|M4H1X6_9REOV Inner capsid protein VP2 OS=Rotavirus F chicken/03V0568/DEU/2003 OX=994994 PE=3 SV=1
MM1 pKa = 6.9 GTYY4 pKa = 10.18 DD5 pKa = 4.56 ALLEE9 pKa = 4.22 EE10 pKa = 5.27 YY11 pKa = 10.28 IHH13 pKa = 6.67 FLYY16 pKa = 10.85 SDD18 pKa = 4.56 DD19 pKa = 4.07 SLSVIPIYY27 pKa = 9.58 YY28 pKa = 8.97 TSNVDD33 pKa = 2.95 IHH35 pKa = 7.08 QEE37 pKa = 3.43 LSKK40 pKa = 11.23 YY41 pKa = 8.19 HH42 pKa = 6.43 TEE44 pKa = 4.11 LIEE47 pKa = 3.97 YY48 pKa = 9.9 NKK50 pKa = 10.26 IKK52 pKa = 10.83 ARR54 pKa = 11.84 TKK56 pKa = 10.06 KK57 pKa = 9.88 HH58 pKa = 6.45 DD59 pKa = 3.78 FNKK62 pKa = 10.51 LEE64 pKa = 4.67 SIHH67 pKa = 6.62 AKK69 pKa = 9.95 YY70 pKa = 10.56 NEE72 pKa = 4.74 SISKK76 pKa = 9.81 SRR78 pKa = 11.84 RR79 pKa = 11.84 LSIVVYY85 pKa = 10.26 AYY87 pKa = 9.99 GKK89 pKa = 9.8 HH90 pKa = 6.18 DD91 pKa = 3.63 AVEE94 pKa = 4.11 RR95 pKa = 11.84 KK96 pKa = 8.65 IVKK99 pKa = 9.88 YY100 pKa = 9.16 ATKK103 pKa = 10.34 KK104 pKa = 10.35 PLIADD109 pKa = 4.37 LTANEE114 pKa = 4.9 LDD116 pKa = 3.81 YY117 pKa = 11.68 EE118 pKa = 4.42 NNKK121 pKa = 8.14 LTKK124 pKa = 10.14 DD125 pKa = 3.38 LYY127 pKa = 10.86 DD128 pKa = 3.65 EE129 pKa = 4.93 KK130 pKa = 11.31 SYY132 pKa = 11.32 TDD134 pKa = 3.81 YY135 pKa = 11.84 LMDD138 pKa = 4.51 PAISTSLTSNLNACMFTIEE157 pKa = 4.43 TEE159 pKa = 4.3 TNEE162 pKa = 4.27 KK163 pKa = 8.94 QRR165 pKa = 11.84 KK166 pKa = 8.57 RR167 pKa = 11.84 YY168 pKa = 9.57 YY169 pKa = 10.33 EE170 pKa = 4.07 LFTIVASTLHH180 pKa = 6.54 HH181 pKa = 6.12 YY182 pKa = 8.84 GIPRR186 pKa = 11.84 HH187 pKa = 5.66 DD188 pKa = 3.4 SKK190 pKa = 11.67 YY191 pKa = 10.11 RR192 pKa = 11.84 YY193 pKa = 8.06 TYY195 pKa = 10.16 EE196 pKa = 3.87 QMRR199 pKa = 11.84 DD200 pKa = 2.95 KK201 pKa = 10.59 PYY203 pKa = 11.12 YY204 pKa = 8.81 IVPWANSAIEE214 pKa = 4.0 MLMSVNNHH222 pKa = 6.62 DD223 pKa = 4.31 DD224 pKa = 3.66 FVIARR229 pKa = 11.84 EE230 pKa = 4.11 LITYY234 pKa = 8.73 SFSNRR239 pKa = 11.84 STLAKK244 pKa = 9.77 IVSSPMSIMTALLDD258 pKa = 3.61 INGTFITDD266 pKa = 3.17 EE267 pKa = 4.17 SLQLQYY273 pKa = 10.9 SSKK276 pKa = 9.86 MIKK279 pKa = 10.55 ALVTDD284 pKa = 3.89 EE285 pKa = 4.29 AKK287 pKa = 10.68 KK288 pKa = 10.36 EE289 pKa = 3.93 LEE291 pKa = 3.96 EE292 pKa = 4.2 MLQKK296 pKa = 10.42 MIDD299 pKa = 3.8 LGLVDD304 pKa = 4.96 IPNILLSWIEE314 pKa = 3.98 NPRR317 pKa = 11.84 LDD319 pKa = 3.65 TFHH322 pKa = 7.26 RR323 pKa = 11.84 VANVYY328 pKa = 9.54 SWSFHH333 pKa = 4.96 VGYY336 pKa = 10.17 RR337 pKa = 11.84 KK338 pKa = 9.6 QLMQDD343 pKa = 3.15 AVQDD347 pKa = 3.92 QLSVEE352 pKa = 4.09 YY353 pKa = 10.73 SEE355 pKa = 5.96 NVDD358 pKa = 2.62 KK359 pKa = 11.23 TMYY362 pKa = 10.49 DD363 pKa = 2.86 EE364 pKa = 4.43 YY365 pKa = 11.51 YY366 pKa = 11.0 NYY368 pKa = 10.03 IKK370 pKa = 10.46 EE371 pKa = 4.17 DD372 pKa = 4.11 YY373 pKa = 10.71 IRR375 pKa = 11.84 MLKK378 pKa = 10.58 DD379 pKa = 3.54 DD380 pKa = 4.71 VIKK383 pKa = 10.25 STTILKK389 pKa = 8.97 QHH391 pKa = 6.22 EE392 pKa = 4.33 LAGLLSMSSASNGKK406 pKa = 9.99 KK407 pKa = 8.97 MEE409 pKa = 4.14 IKK411 pKa = 10.46 FGSKK415 pKa = 10.3 KK416 pKa = 10.72 LFTTRR421 pKa = 11.84 KK422 pKa = 8.47 NMHH425 pKa = 5.69 VMSDD429 pKa = 3.56 VSSGNYY435 pKa = 9.16 NSSIVPPVDD444 pKa = 2.91 ATSPIPLGRR453 pKa = 11.84 RR454 pKa = 11.84 DD455 pKa = 3.43 VPGRR459 pKa = 11.84 RR460 pKa = 11.84 TRR462 pKa = 11.84 VIFILPYY469 pKa = 10.05 EE470 pKa = 4.26 YY471 pKa = 10.73 FLAQHH476 pKa = 7.04 AIVEE480 pKa = 4.38 LLLKK484 pKa = 9.29 QAKK487 pKa = 7.48 HH488 pKa = 4.67 TRR490 pKa = 11.84 EE491 pKa = 3.38 FSEE494 pKa = 5.56 FYY496 pKa = 11.03 SSANQLLSYY505 pKa = 10.05 GDD507 pKa = 3.56 TMRR510 pKa = 11.84 EE511 pKa = 4.2 LNPSTIIIYY520 pKa = 10.06 TDD522 pKa = 3.15 VSQWDD527 pKa = 4.35 GSQHH531 pKa = 4.36 NTQPFRR537 pKa = 11.84 KK538 pKa = 9.54 AIMAAMQDD546 pKa = 3.69 LQAYY550 pKa = 5.96 TTDD553 pKa = 3.62 QNVEE557 pKa = 3.84 RR558 pKa = 11.84 ALNKK562 pKa = 10.15 YY563 pKa = 10.47 YY564 pKa = 8.48 EE565 pKa = 4.36 TQRR568 pKa = 11.84 QLMNSYY574 pKa = 8.42 VTIDD578 pKa = 4.18 DD579 pKa = 5.23 KK580 pKa = 11.09 IIQYY584 pKa = 10.46 GAVASGEE591 pKa = 4.23 KK592 pKa = 7.29 QTKK595 pKa = 8.23 IANSIANLALIKK607 pKa = 9.89 TVLNKK612 pKa = 10.31 LSAKK616 pKa = 8.5 YY617 pKa = 8.72 TFNIKK622 pKa = 10.5 LIRR625 pKa = 11.84 VDD627 pKa = 3.99 GDD629 pKa = 3.57 DD630 pKa = 3.82 NYY632 pKa = 11.49 TVIEE636 pKa = 4.26 SRR638 pKa = 11.84 SQVTQEE644 pKa = 4.25 LLSNLSKK651 pKa = 10.71 DD652 pKa = 3.53 IKK654 pKa = 10.23 DD655 pKa = 3.67 TYY657 pKa = 11.9 ALMNVKK663 pKa = 9.89 VKK665 pKa = 10.91 ALVSTTGLEE674 pKa = 3.79 MAKK677 pKa = 10.03 RR678 pKa = 11.84 YY679 pKa = 9.17 IAGGRR684 pKa = 11.84 IYY686 pKa = 10.48 FRR688 pKa = 11.84 AGINILNNEE697 pKa = 4.19 KK698 pKa = 10.52 RR699 pKa = 11.84 NQQTAWDD706 pKa = 3.89 QAAILYY712 pKa = 10.21 ASYY715 pKa = 9.67 TVNKK719 pKa = 10.03 LRR721 pKa = 11.84 GFITNRR727 pKa = 11.84 TFILTKK733 pKa = 9.8 IMQMTSVKK741 pKa = 10.04 ISGVLRR747 pKa = 11.84 IFPASDD753 pKa = 3.1 VLTLNSTYY761 pKa = 10.98 KK762 pKa = 10.7 VFDD765 pKa = 3.74 EE766 pKa = 3.66 VDD768 pKa = 3.28 YY769 pKa = 11.33 AVNYY773 pKa = 10.21 KK774 pKa = 9.93 VDD776 pKa = 3.95 ADD778 pKa = 3.83 SLMLQKK784 pKa = 11.04 LLIKK788 pKa = 10.56 VSQSRR793 pKa = 11.84 SKK795 pKa = 11.09 VADD798 pKa = 4.66 EE799 pKa = 4.24 ISKK802 pKa = 9.87 SHH804 pKa = 6.45 IFSNYY809 pKa = 7.11 VKK811 pKa = 10.6 YY812 pKa = 10.73 ISTMLLKK819 pKa = 10.34 IPNKK823 pKa = 9.95 TIEE826 pKa = 4.03 QGLAKK831 pKa = 9.86 TEE833 pKa = 4.0 KK834 pKa = 10.48 AKK836 pKa = 11.0 LNSYY840 pKa = 9.99 PPISLEE846 pKa = 4.12 KK847 pKa = 10.56 RR848 pKa = 11.84 KK849 pKa = 7.8 TQLDD853 pKa = 3.95 TLIKK857 pKa = 8.58 TLQYY861 pKa = 10.13 PIIYY865 pKa = 10.12 VGGKK869 pKa = 5.13 TTLNDD874 pKa = 3.19 VVSVVDD880 pKa = 3.52 QYY882 pKa = 12.22 VEE884 pKa = 4.12 YY885 pKa = 10.94 KK886 pKa = 10.88 SIDD889 pKa = 3.45 PEE891 pKa = 4.03 KK892 pKa = 10.46 KK893 pKa = 8.7 YY894 pKa = 11.0 RR895 pKa = 11.84 NYY897 pKa = 11.16 SPMLPTDD904 pKa = 3.07 ISEE907 pKa = 4.48 VITVIGSRR915 pKa = 11.84 LSSYY919 pKa = 10.1 EE920 pKa = 3.68 DD921 pKa = 3.37 HH922 pKa = 6.96 AGKK925 pKa = 10.2 SAIAKK930 pKa = 9.41 LISTYY935 pKa = 10.44 SVYY938 pKa = 10.48 RR939 pKa = 11.84 PSIEE943 pKa = 3.78 EE944 pKa = 4.09 LYY946 pKa = 10.93 AIITKK951 pKa = 10.22 RR952 pKa = 11.84 EE953 pKa = 3.95 NEE955 pKa = 3.77 IKK957 pKa = 10.5 LYY959 pKa = 9.26 MLSLSVPLQDD969 pKa = 2.46 VDD971 pKa = 5.3 AYY973 pKa = 9.97 IASQTYY979 pKa = 9.99 KK980 pKa = 9.84 RR981 pKa = 11.84 DD982 pKa = 3.05 KK983 pKa = 11.01 YY984 pKa = 11.07 LILQSYY990 pKa = 10.18 VYY992 pKa = 10.86 DD993 pKa = 4.12 LLSINYY999 pKa = 8.58 GAYY1002 pKa = 9.93 QLFDD1006 pKa = 3.9 FEE1008 pKa = 6.1 SEE1010 pKa = 4.25 FFSKK1014 pKa = 10.89 YY1015 pKa = 5.71 ITYY1018 pKa = 10.59 VAMSKK1023 pKa = 10.32 VPSAEE1028 pKa = 4.03 FLVGTYY1034 pKa = 10.41 VKK1036 pKa = 10.68 LKK1038 pKa = 10.25 IINRR1042 pKa = 11.84 YY1043 pKa = 5.86 LTEE1046 pKa = 4.31 RR1047 pKa = 11.84 KK1048 pKa = 8.79 WYY1050 pKa = 9.11 SANITIPKK1058 pKa = 8.8 HH1059 pKa = 5.85 EE1060 pKa = 4.11 YY1061 pKa = 9.43 MKK1063 pKa = 10.51 INKK1066 pKa = 7.43 LTWEE1070 pKa = 4.15 ISSVRR1075 pKa = 11.84 SPYY1078 pKa = 9.55 TSANFFQEE1086 pKa = 3.91
Molecular weight: 125.38 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.155
IPC2_protein 8.317
IPC_protein 8.185
Toseland 8.697
ProMoST 8.624
Dawson 9.063
Bjellqvist 8.814
Wikipedia 9.297
Rodwell 9.194
Grimsley 9.18
Solomon 9.092
Lehninger 9.033
Nozaki 8.697
DTASelect 8.814
Thurlkill 8.873
EMBOSS 9.151
Sillero 8.99
Patrickios 4.075
IPC_peptide 9.077
IPC2_peptide 7.366
IPC2.peptide.svr19 7.591
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
11
0
11
5735
169
1086
521.4
59.89
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.242 ± 0.352
1.255 ± 0.399
5.754 ± 0.286
5.841 ± 0.633
3.854 ± 0.367
3.4 ± 0.321
1.779 ± 0.274
7.463 ± 0.366
6.556 ± 0.603
8.596 ± 0.527
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.173 ± 0.238
6.207 ± 0.463
2.964 ± 0.294
4.725 ± 0.388
4.917 ± 0.364
7.393 ± 0.597
7.027 ± 0.351
6.643 ± 0.368
0.837 ± 0.101
5.371 ± 0.518
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here