Pusillimonas sp. YR330

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Alcaligenaceae; Pusillimonas; unclassified Pusillimonas

Average proteome isoelectric point is 6.67

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3724 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4R1LH01|A0A4R1LH01_9BURK Aminotransferase OS=Pusillimonas sp. YR330 OX=2135726 GN=C8K33_2963 PE=3 SV=1
MM1 pKa = 7.25NASSHH6 pKa = 5.17IVEE9 pKa = 4.39TLEE12 pKa = 4.24HH13 pKa = 6.67GLVLDD18 pKa = 3.75VTIEE22 pKa = 3.98DD23 pKa = 3.93LQFRR27 pKa = 11.84AYY29 pKa = 10.56VVISEE34 pKa = 4.14PDD36 pKa = 3.13INLVADD42 pKa = 4.39FVPRR46 pKa = 11.84EE47 pKa = 3.95QFEE50 pKa = 4.12QDD52 pKa = 2.9GDD54 pKa = 3.96VHH56 pKa = 6.46VAAIYY61 pKa = 10.48SPEE64 pKa = 4.0EE65 pKa = 3.7VHH67 pKa = 6.84EE68 pKa = 4.33QIQDD72 pKa = 2.89ITFNMNLGDD81 pKa = 3.98AAVFLCTSQQVYY93 pKa = 10.96SDD95 pKa = 3.61ALEE98 pKa = 4.09EE99 pKa = 4.36LGQNLTTQQ107 pKa = 3.46

Molecular weight: 11.98 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4R1LNG7|A0A4R1LNG7_9BURK Uncharacterized protein OS=Pusillimonas sp. YR330 OX=2135726 GN=C8K33_1312 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVTRR11 pKa = 11.84RR12 pKa = 11.84KK13 pKa = 8.0RR14 pKa = 11.84THH16 pKa = 5.76GFRR19 pKa = 11.84VRR21 pKa = 11.84MKK23 pKa = 9.39TRR25 pKa = 11.84GGRR28 pKa = 11.84AVINARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.58GRR39 pKa = 11.84KK40 pKa = 8.75RR41 pKa = 11.84LAVV44 pKa = 3.41

Molecular weight: 5.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3724

0

3724

1229091

29

3178

330.0

36.0

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.299 ± 0.042

0.933 ± 0.012

5.278 ± 0.036

5.126 ± 0.034

3.667 ± 0.024

8.004 ± 0.048

2.202 ± 0.021

5.448 ± 0.028

3.837 ± 0.035

10.649 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.634 ± 0.02

3.158 ± 0.024

4.923 ± 0.026

4.222 ± 0.026

5.873 ± 0.041

6.017 ± 0.029

5.321 ± 0.03

7.437 ± 0.038

1.425 ± 0.017

2.548 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski