Amino acid dipepetide frequency for Pusillimonas sp. YR330

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
13.872AlaAla: 13.872 ± 0.168
1.203AlaCys: 1.203 ± 0.032
5.887AlaAsp: 5.887 ± 0.083
5.538AlaGlu: 5.538 ± 0.066
3.794AlaPhe: 3.794 ± 0.059
9.337AlaGly: 9.337 ± 0.093
2.312AlaHis: 2.312 ± 0.04
6.277AlaIle: 6.277 ± 0.088
4.129AlaLys: 4.129 ± 0.073
12.919AlaLeu: 12.919 ± 0.132
3.326AlaMet: 3.326 ± 0.058
3.223AlaAsn: 3.223 ± 0.051
4.76AlaPro: 4.76 ± 0.074
5.342AlaGln: 5.342 ± 0.072
6.525AlaArg: 6.525 ± 0.08
6.769AlaSer: 6.769 ± 0.087
5.446AlaThr: 5.446 ± 0.072
7.947AlaVal: 7.947 ± 0.095
1.677AlaTrp: 1.677 ± 0.043
2.703AlaTyr: 2.703 ± 0.054
0.0AlaXaa: 0.0 ± 0.0
Cys
1.017CysAla: 1.017 ± 0.031
0.133CysCys: 0.133 ± 0.011
0.473CysAsp: 0.473 ± 0.022
0.451CysGlu: 0.451 ± 0.019
0.353CysPhe: 0.353 ± 0.019
0.951CysGly: 0.951 ± 0.03
0.261CysHis: 0.261 ± 0.014
0.476CysIle: 0.476 ± 0.02
0.256CysLys: 0.256 ± 0.016
0.897CysLeu: 0.897 ± 0.027
0.231CysMet: 0.231 ± 0.013
0.308CysAsn: 0.308 ± 0.017
0.461CysPro: 0.461 ± 0.021
0.345CysGln: 0.345 ± 0.016
0.531CysArg: 0.531 ± 0.019
0.602CysSer: 0.602 ± 0.023
0.454CysThr: 0.454 ± 0.018
0.741CysVal: 0.741 ± 0.025
0.14CysTrp: 0.14 ± 0.012
0.243CysTyr: 0.243 ± 0.013
0.0CysXaa: 0.0 ± 0.0
Asp
5.839AspAla: 5.839 ± 0.089
0.477AspCys: 0.477 ± 0.021
2.726AspAsp: 2.726 ± 0.059
2.944AspGlu: 2.944 ± 0.054
2.163AspPhe: 2.163 ± 0.044
4.123AspGly: 4.123 ± 0.089
1.116AspHis: 1.116 ± 0.03
3.327AspIle: 3.327 ± 0.054
2.084AspLys: 2.084 ± 0.051
5.34AspLeu: 5.34 ± 0.074
1.403AspMet: 1.403 ± 0.035
1.513AspAsn: 1.513 ± 0.044
2.811AspPro: 2.811 ± 0.049
1.953AspGln: 1.953 ± 0.037
2.748AspArg: 2.748 ± 0.052
2.761AspSer: 2.761 ± 0.05
2.924AspThr: 2.924 ± 0.06
4.003AspVal: 4.003 ± 0.059
0.897AspTrp: 0.897 ± 0.027
1.626AspTyr: 1.626 ± 0.039
0.0AspXaa: 0.0 ± 0.0
Glu
5.504GluAla: 5.504 ± 0.07
0.404GluCys: 0.404 ± 0.018
2.191GluAsp: 2.191 ± 0.046
2.409GluGlu: 2.409 ± 0.051
1.879GluPhe: 1.879 ± 0.04
3.406GluGly: 3.406 ± 0.059
1.371GluHis: 1.371 ± 0.039
3.032GluIle: 3.032 ± 0.062
2.324GluLys: 2.324 ± 0.052
5.59GluLeu: 5.59 ± 0.073
1.303GluMet: 1.303 ± 0.031
1.774GluAsn: 1.774 ± 0.039
2.361GluPro: 2.361 ± 0.043
2.847GluGln: 2.847 ± 0.055
3.747GluArg: 3.747 ± 0.064
2.899GluSer: 2.899 ± 0.052
2.701GluThr: 2.701 ± 0.042
3.58GluVal: 3.58 ± 0.063
0.705GluTrp: 0.705 ± 0.025
1.235GluTyr: 1.235 ± 0.031
0.0GluXaa: 0.0 ± 0.0
Phe
3.751PheAla: 3.751 ± 0.055
0.416PheCys: 0.416 ± 0.02
2.458PheAsp: 2.458 ± 0.046
1.973PheGlu: 1.973 ± 0.042
1.527PhePhe: 1.527 ± 0.039
3.306PheGly: 3.306 ± 0.055
0.703PheHis: 0.703 ± 0.024
1.996PheIle: 1.996 ± 0.047
1.384PheLys: 1.384 ± 0.034
3.309PheLeu: 3.309 ± 0.053
0.967PheMet: 0.967 ± 0.027
1.342PheAsn: 1.342 ± 0.032
1.62PhePro: 1.62 ± 0.034
1.215PheGln: 1.215 ± 0.035
1.79PheArg: 1.79 ± 0.042
2.6PheSer: 2.6 ± 0.047
2.068PheThr: 2.068 ± 0.042
2.671PheVal: 2.671 ± 0.053
0.564PheTrp: 0.564 ± 0.021
1.011PheTyr: 1.011 ± 0.031
0.0PheXaa: 0.0 ± 0.0
Gly
8.056GlyAla: 8.056 ± 0.091
0.868GlyCys: 0.868 ± 0.028
3.708GlyAsp: 3.708 ± 0.072
3.806GlyGlu: 3.806 ± 0.063
3.158GlyPhe: 3.158 ± 0.05
6.352GlyGly: 6.352 ± 0.13
1.838GlyHis: 1.838 ± 0.039
4.782GlyIle: 4.782 ± 0.069
3.848GlyLys: 3.848 ± 0.071
8.858GlyLeu: 8.858 ± 0.089
2.48GlyMet: 2.48 ± 0.048
2.527GlyAsn: 2.527 ± 0.058
2.9GlyPro: 2.9 ± 0.05
3.336GlyGln: 3.336 ± 0.054
4.491GlyArg: 4.491 ± 0.063
4.773GlySer: 4.773 ± 0.068
4.324GlyThr: 4.324 ± 0.072
6.138GlyVal: 6.138 ± 0.074
1.289GlyTrp: 1.289 ± 0.036
2.508GlyTyr: 2.508 ± 0.05
0.0GlyXaa: 0.0 ± 0.0
His
2.478HisAla: 2.478 ± 0.043
0.264HisCys: 0.264 ± 0.016
1.208HisAsp: 1.208 ± 0.034
1.153HisGlu: 1.153 ± 0.032
0.779HisPhe: 0.779 ± 0.027
1.923HisGly: 1.923 ± 0.044
0.589HisHis: 0.589 ± 0.021
1.269HisIle: 1.269 ± 0.032
0.714HisLys: 0.714 ± 0.024
2.05HisLeu: 2.05 ± 0.047
0.548HisMet: 0.548 ± 0.022
0.689HisAsn: 0.689 ± 0.026
1.46HisPro: 1.46 ± 0.037
0.848HisGln: 0.848 ± 0.024
1.224HisArg: 1.224 ± 0.034
1.203HisSer: 1.203 ± 0.031
1.147HisThr: 1.147 ± 0.033
1.481HisVal: 1.481 ± 0.041
0.325HisTrp: 0.325 ± 0.016
0.666HisTyr: 0.666 ± 0.025
0.0HisXaa: 0.0 ± 0.0
Ile
6.733IleAla: 6.733 ± 0.087
0.512IleCys: 0.512 ± 0.02
3.387IleAsp: 3.387 ± 0.057
3.341IleGlu: 3.341 ± 0.057
1.843IlePhe: 1.843 ± 0.048
4.733IleGly: 4.733 ± 0.074
1.041IleHis: 1.041 ± 0.029
2.625IleIle: 2.625 ± 0.052
2.263IleLys: 2.263 ± 0.049
4.684IleLeu: 4.684 ± 0.072
1.203IleMet: 1.203 ± 0.035
1.954IleAsn: 1.954 ± 0.039
2.614IlePro: 2.614 ± 0.044
1.925IleGln: 1.925 ± 0.038
2.832IleArg: 2.832 ± 0.05
3.512IleSer: 3.512 ± 0.056
3.154IleThr: 3.154 ± 0.063
4.265IleVal: 4.265 ± 0.066
0.641IleTrp: 0.641 ± 0.024
1.221IleTyr: 1.221 ± 0.034
0.0IleXaa: 0.0 ± 0.0
Lys
4.236LysAla: 4.236 ± 0.074
0.162LysCys: 0.162 ± 0.011
1.977LysAsp: 1.977 ± 0.046
2.017LysGlu: 2.017 ± 0.044
1.063LysPhe: 1.063 ± 0.034
2.773LysGly: 2.773 ± 0.054
0.86LysHis: 0.86 ± 0.028
1.981LysIle: 1.981 ± 0.047
1.925LysLys: 1.925 ± 0.051
4.025LysLeu: 4.025 ± 0.059
1.028LysMet: 1.028 ± 0.033
1.333LysAsn: 1.333 ± 0.036
2.269LysPro: 2.269 ± 0.054
1.716LysGln: 1.716 ± 0.039
2.329LysArg: 2.329 ± 0.045
2.18LysSer: 2.18 ± 0.041
2.354LysThr: 2.354 ± 0.048
2.824LysVal: 2.824 ± 0.059
0.479LysTrp: 0.479 ± 0.018
0.842LysTyr: 0.842 ± 0.029
0.0LysXaa: 0.0 ± 0.0
Leu
13.415LeuAla: 13.415 ± 0.15
1.075LeuCys: 1.075 ± 0.033
5.917LeuAsp: 5.917 ± 0.082
5.221LeuGlu: 5.221 ± 0.069
3.8LeuPhe: 3.8 ± 0.061
8.452LeuGly: 8.452 ± 0.086
2.322LeuHis: 2.322 ± 0.041
5.454LeuIle: 5.454 ± 0.076
3.918LeuLys: 3.918 ± 0.059
11.138LeuLeu: 11.138 ± 0.152
2.639LeuMet: 2.639 ± 0.048
3.298LeuAsn: 3.298 ± 0.054
5.718LeuPro: 5.718 ± 0.075
3.992LeuGln: 3.992 ± 0.058
6.498LeuArg: 6.498 ± 0.081
7.027LeuSer: 7.027 ± 0.078
5.541LeuThr: 5.541 ± 0.072
7.563LeuVal: 7.563 ± 0.09
1.22LeuTrp: 1.22 ± 0.034
2.28LeuTyr: 2.28 ± 0.042
0.0LeuXaa: 0.0 ± 0.0
Met
3.087MetAla: 3.087 ± 0.047
0.199MetCys: 0.199 ± 0.013
1.193MetAsp: 1.193 ± 0.031
1.112MetGlu: 1.112 ± 0.033
0.828MetPhe: 0.828 ± 0.026
1.982MetGly: 1.982 ± 0.05
0.533MetHis: 0.533 ± 0.02
1.246MetIle: 1.246 ± 0.035
1.089MetLys: 1.089 ± 0.03
2.956MetLeu: 2.956 ± 0.05
0.697MetMet: 0.697 ± 0.021
0.904MetAsn: 0.904 ± 0.031
1.535MetPro: 1.535 ± 0.036
1.276MetGln: 1.276 ± 0.031
1.676MetArg: 1.676 ± 0.036
1.811MetSer: 1.811 ± 0.041
1.669MetThr: 1.669 ± 0.034
1.855MetVal: 1.855 ± 0.046
0.216MetTrp: 0.216 ± 0.013
0.475MetTyr: 0.475 ± 0.018
0.0MetXaa: 0.0 ± 0.0
Asn
3.502AsnAla: 3.502 ± 0.059
0.289AsnCys: 0.289 ± 0.016
1.662AsnAsp: 1.662 ± 0.043
1.591AsnGlu: 1.591 ± 0.041
1.075AsnPhe: 1.075 ± 0.028
2.591AsnGly: 2.591 ± 0.061
0.622AsnHis: 0.622 ± 0.019
1.817AsnIle: 1.817 ± 0.04
1.266AsnLys: 1.266 ± 0.031
3.062AsnLeu: 3.062 ± 0.057
0.784AsnMet: 0.784 ± 0.028
1.085AsnAsn: 1.085 ± 0.031
2.071AsnPro: 2.071 ± 0.044
1.181AsnGln: 1.181 ± 0.034
1.83AsnArg: 1.83 ± 0.042
1.691AsnSer: 1.691 ± 0.039
1.902AsnThr: 1.902 ± 0.041
2.211AsnVal: 2.211 ± 0.047
0.491AsnTrp: 0.491 ± 0.021
0.851AsnTyr: 0.851 ± 0.027
0.0AsnXaa: 0.0 ± 0.0
Pro
5.617ProAla: 5.617 ± 0.078
0.363ProCys: 0.363 ± 0.017
3.262ProAsp: 3.262 ± 0.048
3.337ProGlu: 3.337 ± 0.051
1.824ProPhe: 1.824 ± 0.04
4.115ProGly: 4.115 ± 0.063
1.012ProHis: 1.012 ± 0.028
2.468ProIle: 2.468 ± 0.039
1.769ProLys: 1.769 ± 0.046
4.862ProLeu: 4.862 ± 0.061
1.243ProMet: 1.243 ± 0.031
1.534ProAsn: 1.534 ± 0.035
2.004ProPro: 2.004 ± 0.051
1.955ProGln: 1.955 ± 0.04
2.267ProArg: 2.267 ± 0.048
2.859ProSer: 2.859 ± 0.054
2.464ProThr: 2.464 ± 0.04
4.133ProVal: 4.133 ± 0.062
0.734ProTrp: 0.734 ± 0.028
1.411ProTyr: 1.411 ± 0.035
0.0ProXaa: 0.0 ± 0.0
Gln
5.933GlnAla: 5.933 ± 0.073
0.286GlnCys: 0.286 ± 0.015
1.935GlnAsp: 1.935 ± 0.04
2.062GlnGlu: 2.062 ± 0.042
1.329GlnPhe: 1.329 ± 0.028
3.315GlnGly: 3.315 ± 0.051
1.088GlnHis: 1.088 ± 0.031
2.097GlnIle: 2.097 ± 0.046
1.425GlnLys: 1.425 ± 0.035
4.178GlnLeu: 4.178 ± 0.07
0.988GlnMet: 0.988 ± 0.03
1.187GlnAsn: 1.187 ± 0.033
2.028GlnPro: 2.028 ± 0.042
2.224GlnGln: 2.224 ± 0.052
2.94GlnArg: 2.94 ± 0.058
2.267GlnSer: 2.267 ± 0.049
2.158GlnThr: 2.158 ± 0.045
3.098GlnVal: 3.098 ± 0.05
0.665GlnTrp: 0.665 ± 0.022
1.017GlnTyr: 1.017 ± 0.03
0.0GlnXaa: 0.0 ± 0.0
Arg
5.645ArgAla: 5.645 ± 0.07
0.535ArgCys: 0.535 ± 0.023
3.0ArgAsp: 3.0 ± 0.052
3.204ArgGlu: 3.204 ± 0.061
2.437ArgPhe: 2.437 ± 0.047
3.745ArgGly: 3.745 ± 0.062
1.526ArgHis: 1.526 ± 0.035
3.557ArgIle: 3.557 ± 0.048
2.294ArgLys: 2.294 ± 0.046
6.711ArgLeu: 6.711 ± 0.092
1.644ArgMet: 1.644 ± 0.034
1.985ArgAsn: 1.985 ± 0.043
2.599ArgPro: 2.599 ± 0.047
2.769ArgGln: 2.769 ± 0.048
3.909ArgArg: 3.909 ± 0.067
3.326ArgSer: 3.326 ± 0.055
2.816ArgThr: 2.816 ± 0.054
4.224ArgVal: 4.224 ± 0.062
0.9ArgTrp: 0.9 ± 0.031
1.901ArgTyr: 1.901 ± 0.044
0.0ArgXaa: 0.0 ± 0.0
Ser
6.298SerAla: 6.298 ± 0.089
0.509SerCys: 0.509 ± 0.023
3.073SerAsp: 3.073 ± 0.054
2.729SerGlu: 2.729 ± 0.049
2.416SerPhe: 2.416 ± 0.043
5.515SerGly: 5.515 ± 0.066
1.322SerHis: 1.322 ± 0.032
3.355SerIle: 3.355 ± 0.058
2.08SerLys: 2.08 ± 0.046
6.565SerLeu: 6.565 ± 0.081
1.676SerMet: 1.676 ± 0.039
1.875SerAsn: 1.875 ± 0.047
2.961SerPro: 2.961 ± 0.054
2.388SerGln: 2.388 ± 0.039
3.485SerArg: 3.485 ± 0.055
3.816SerSer: 3.816 ± 0.066
3.232SerThr: 3.232 ± 0.05
4.428SerVal: 4.428 ± 0.064
0.937SerTrp: 0.937 ± 0.028
1.514SerTyr: 1.514 ± 0.041
0.0SerXaa: 0.0 ± 0.0
Thr
5.588ThrAla: 5.588 ± 0.067
0.424ThrCys: 0.424 ± 0.018
2.761ThrAsp: 2.761 ± 0.059
2.656ThrGlu: 2.656 ± 0.049
1.799ThrPhe: 1.799 ± 0.042
4.628ThrGly: 4.628 ± 0.066
1.211ThrHis: 1.211 ± 0.035
2.866ThrIle: 2.866 ± 0.062
1.508ThrLys: 1.508 ± 0.04
6.289ThrLeu: 6.289 ± 0.071
1.259ThrMet: 1.259 ± 0.029
1.478ThrAsn: 1.478 ± 0.036
3.398ThrPro: 3.398 ± 0.054
2.204ThrGln: 2.204 ± 0.044
3.022ThrArg: 3.022 ± 0.05
3.049ThrSer: 3.049 ± 0.045
2.87ThrThr: 2.87 ± 0.054
4.297ThrVal: 4.297 ± 0.069
0.7ThrTrp: 0.7 ± 0.025
1.206ThrTyr: 1.206 ± 0.031
0.0ThrXaa: 0.0 ± 0.0
Val
8.432ValAla: 8.432 ± 0.093
0.762ValCys: 0.762 ± 0.024
3.968ValAsp: 3.968 ± 0.057
3.787ValGlu: 3.787 ± 0.06
2.97ValPhe: 2.97 ± 0.053
5.527ValGly: 5.527 ± 0.064
1.472ValHis: 1.472 ± 0.038
4.03ValIle: 4.03 ± 0.06
2.735ValLys: 2.735 ± 0.052
8.3ValLeu: 8.3 ± 0.101
1.938ValMet: 1.938 ± 0.036
2.363ValAsn: 2.363 ± 0.046
3.64ValPro: 3.64 ± 0.064
2.875ValGln: 2.875 ± 0.052
4.073ValArg: 4.073 ± 0.061
4.74ValSer: 4.74 ± 0.067
3.897ValThr: 3.897 ± 0.071
6.126ValVal: 6.126 ± 0.078
0.993ValTrp: 0.993 ± 0.033
1.744ValTyr: 1.744 ± 0.039
0.0ValXaa: 0.0 ± 0.0
Trp
1.267TrpAla: 1.267 ± 0.034
0.149TrpCys: 0.149 ± 0.01
0.582TrpAsp: 0.582 ± 0.023
0.596TrpGlu: 0.596 ± 0.022
0.6TrpPhe: 0.6 ± 0.021
0.979TrpGly: 0.979 ± 0.034
0.354TrpHis: 0.354 ± 0.017
0.708TrpIle: 0.708 ± 0.023
0.484TrpLys: 0.484 ± 0.02
2.04TrpLeu: 2.04 ± 0.044
0.422TrpMet: 0.422 ± 0.017
0.488TrpAsn: 0.488 ± 0.023
0.679TrpPro: 0.679 ± 0.026
0.764TrpGln: 0.764 ± 0.025
1.074TrpArg: 1.074 ± 0.031
0.817TrpSer: 0.817 ± 0.025
0.663TrpThr: 0.663 ± 0.023
0.985TrpVal: 0.985 ± 0.028
0.254TrpTrp: 0.254 ± 0.016
0.346TrpTyr: 0.346 ± 0.016
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.717TyrAla: 2.717 ± 0.048
0.299TyrCys: 0.299 ± 0.017
1.409TyrAsp: 1.409 ± 0.037
1.335TyrGlu: 1.335 ± 0.032
1.032TyrPhe: 1.032 ± 0.028
2.288TyrGly: 2.288 ± 0.044
0.464TyrHis: 0.464 ± 0.021
1.122TyrIle: 1.122 ± 0.031
0.879TyrLys: 0.879 ± 0.029
2.713TyrLeu: 2.713 ± 0.049
0.56TyrMet: 0.56 ± 0.022
0.714TyrAsn: 0.714 ± 0.028
1.343TyrPro: 1.343 ± 0.037
1.064TyrGln: 1.064 ± 0.029
1.737TyrArg: 1.737 ± 0.036
1.47TyrSer: 1.47 ± 0.036
1.428TyrThr: 1.428 ± 0.033
1.801TyrVal: 1.801 ± 0.035
0.424TyrTrp: 0.424 ± 0.019
0.679TyrTyr: 0.679 ± 0.028
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3724 proteins (1229091 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski