Sphingomonas sp.

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Sphingomonadaceae; Sphingomonas; unclassified Sphingomonas

Average proteome isoelectric point is 6.67

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3455 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A520EKZ8|A0A520EKZ8_SPHSX DNA-directed RNA polymerase (Fragment) OS=Sphingomonas sp. OX=28214 GN=EOP66_16400 PE=4 SV=1
QQ1 pKa = 7.2FGSNVIDD8 pKa = 4.21GEE10 pKa = 4.41IQFSSTFDD18 pKa = 3.46FDD20 pKa = 5.22FDD22 pKa = 3.91PTDD25 pKa = 4.57GISAGTYY32 pKa = 10.46DD33 pKa = 4.63FIGVAIHH40 pKa = 6.37EE41 pKa = 4.9LGHH44 pKa = 6.78ALGFLSGVEE53 pKa = 4.38DD54 pKa = 4.14FDD56 pKa = 5.19FSVGGGFPVDD66 pKa = 4.36DD67 pKa = 4.03YY68 pKa = 10.78WWGYY72 pKa = 8.07GADD75 pKa = 3.66MFRR78 pKa = 11.84YY79 pKa = 8.86SAPGKK84 pKa = 10.2LDD86 pKa = 3.14WTFGTDD92 pKa = 3.65SYY94 pKa = 11.68FSLDD98 pKa = 3.57GGDD101 pKa = 3.37TAFMGGYY108 pKa = 9.2WSTGSNFGDD117 pKa = 3.72GWQASHH123 pKa = 6.24WKK125 pKa = 10.23EE126 pKa = 3.75PAQSCGDD133 pKa = 3.49FLGIMNPYY141 pKa = 8.73ICSGLEE147 pKa = 4.01DD148 pKa = 3.57QVEE151 pKa = 4.2ALDD154 pKa = 5.03LALLDD159 pKa = 4.71AIGWNVNVDD168 pKa = 3.3VLANPGYY175 pKa = 9.15TFTTGAAFGLVPEE188 pKa = 4.33PTTWAMMIGGMGLVGGAMRR207 pKa = 11.84RR208 pKa = 11.84RR209 pKa = 11.84RR210 pKa = 11.84TSVRR214 pKa = 11.84FATAKK219 pKa = 10.44AA220 pKa = 3.41

Molecular weight: 23.69 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A520ENI6|A0A520ENI6_SPHSX 50S ribosomal protein L16 (Fragment) OS=Sphingomonas sp. OX=28214 GN=rplP PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84ARR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.37GFRR19 pKa = 11.84ARR21 pKa = 11.84MATVGGRR28 pKa = 11.84AVIHH32 pKa = 6.17ARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.02KK41 pKa = 10.61LSAA44 pKa = 4.03

Molecular weight: 5.03 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3455

0

3455

776787

22

1323

224.8

24.4

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.511 ± 0.059

0.795 ± 0.014

6.143 ± 0.037

5.007 ± 0.043

3.56 ± 0.027

8.56 ± 0.044

2.071 ± 0.026

5.026 ± 0.03

3.174 ± 0.035

9.582 ± 0.056

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.452 ± 0.026

2.706 ± 0.035

5.152 ± 0.031

3.195 ± 0.027

7.291 ± 0.04

5.512 ± 0.037

5.918 ± 0.042

7.443 ± 0.032

1.446 ± 0.021

2.455 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski