Adhaeribacter aerolatus
Average proteome isoelectric point is 6.86
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5168 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A512AST3|A0A512AST3_9BACT PINc domain-containing protein OS=Adhaeribacter aerolatus OX=670289 GN=AAE02nite_04370 PE=4 SV=1
MM1 pKa = 7.7 NDD3 pKa = 2.49 IKK5 pKa = 10.79 IYY7 pKa = 8.94 VQEE10 pKa = 4.1 PSGDD14 pKa = 3.68 KK15 pKa = 10.7 TPVDD19 pKa = 4.01 APTDD23 pKa = 3.47 MGLSLMEE30 pKa = 3.91 VLKK33 pKa = 11.16 ASGYY37 pKa = 9.79 PIAATCGGMALCATCHH53 pKa = 6.23 IEE55 pKa = 4.03 VLSDD59 pKa = 3.71 HH60 pKa = 6.62 EE61 pKa = 5.05 LPPPSDD67 pKa = 3.78 DD68 pKa = 3.3 EE69 pKa = 6.34 GYY71 pKa = 9.69 MLDD74 pKa = 4.03 SLPAVTDD81 pKa = 3.55 NSRR84 pKa = 11.84 LSCQVRR90 pKa = 11.84 LTPEE94 pKa = 3.99 LDD96 pKa = 3.46 EE97 pKa = 5.34 LVVKK101 pKa = 10.75 LGAGDD106 pKa = 3.45
Molecular weight: 11.25 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.799
IPC2_protein 4.024
IPC_protein 3.973
Toseland 3.77
ProMoST 4.062
Dawson 3.961
Bjellqvist 4.177
Wikipedia 3.897
Rodwell 3.808
Grimsley 3.681
Solomon 3.948
Lehninger 3.91
Nozaki 4.088
DTASelect 4.304
Thurlkill 3.834
EMBOSS 3.91
Sillero 4.101
Patrickios 1.939
IPC_peptide 3.948
IPC2_peptide 4.075
IPC2.peptide.svr19 3.999
Protein with the highest isoelectric point:
>tr|A0A512AZT9|A0A512AZT9_9BACT Uncharacterized protein OS=Adhaeribacter aerolatus OX=670289 GN=AAE02nite_28950 PE=4 SV=1
MM1 pKa = 7.41 KK2 pKa = 10.34 KK3 pKa = 10.23 IIATLGVAFIFSTVQAQTTKK23 pKa = 10.59 SVPAQTRR30 pKa = 11.84 PEE32 pKa = 4.2 KK33 pKa = 10.34 NNHH36 pKa = 4.58 GTVVSTAAHH45 pKa = 3.94 QTYY48 pKa = 8.86 PQSEE52 pKa = 4.16 KK53 pKa = 10.87 GKK55 pKa = 9.55 FISRR59 pKa = 11.84 IARR62 pKa = 11.84 SKK64 pKa = 10.66 SRR66 pKa = 11.84 IVGAKK71 pKa = 9.03 KK72 pKa = 10.22 QPTVNPKK79 pKa = 10.08 KK80 pKa = 10.64 LSTSKK85 pKa = 10.26 PQNPKK90 pKa = 10.92 AKK92 pKa = 9.41 GLQINRR98 pKa = 11.84 PARR101 pKa = 11.84 PVPAQRR107 pKa = 11.84 RR108 pKa = 11.84 ILVQRR113 pKa = 11.84 PTSKK117 pKa = 10.55 GQSVRR122 pKa = 11.84 LNRR125 pKa = 11.84 PNPVRR130 pKa = 11.84 RR131 pKa = 11.84 PVGNGRR137 pKa = 11.84 FTQVGRR143 pKa = 11.84 GG144 pKa = 3.37
Molecular weight: 15.79 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.344
IPC2_protein 10.862
IPC_protein 12.281
Toseland 12.457
ProMoST 12.939
Dawson 12.471
Bjellqvist 12.442
Wikipedia 12.925
Rodwell 12.31
Grimsley 12.501
Solomon 12.954
Lehninger 12.852
Nozaki 12.457
DTASelect 12.442
Thurlkill 12.457
EMBOSS 12.954
Sillero 12.457
Patrickios 12.032
IPC_peptide 12.954
IPC2_peptide 11.93
IPC2.peptide.svr19 9.009
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5168
0
5168
1833445
44
2721
354.8
39.68
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.165 ± 0.032
0.698 ± 0.011
4.874 ± 0.023
5.854 ± 0.035
4.678 ± 0.024
7.026 ± 0.037
1.866 ± 0.015
6.479 ± 0.032
6.252 ± 0.033
9.964 ± 0.039
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.077 ± 0.017
5.527 ± 0.033
4.418 ± 0.022
4.361 ± 0.027
4.414 ± 0.026
5.692 ± 0.026
5.982 ± 0.038
6.431 ± 0.023
1.241 ± 0.014
4.001 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here