Sweet potato leaf curl Hubei virus
Average proteome isoelectric point is 7.81
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A345GSG2|A0A345GSG2_9GEMI Protein V2 OS=Sweet potato leaf curl Hubei virus OX=2282485 PE=3 SV=1
MM1 pKa = 7.17 EE2 pKa = 4.15 QSLWDD7 pKa = 4.38 PLTHH11 pKa = 6.93 PLPEE15 pKa = 4.21 TLHH18 pKa = 6.51 GFRR21 pKa = 11.84 CMLSLKK27 pKa = 10.18 YY28 pKa = 10.11 MSNIRR33 pKa = 11.84 DD34 pKa = 3.47 KK35 pKa = 11.56 YY36 pKa = 9.73 EE37 pKa = 4.39 PGTLGHH43 pKa = 6.61 EE44 pKa = 4.23 LAVLLIRR51 pKa = 11.84 SLRR54 pKa = 11.84 GKK56 pKa = 10.16 NYY58 pKa = 9.19 VRR60 pKa = 11.84 STSSYY65 pKa = 10.99 EE66 pKa = 3.99 EE67 pKa = 4.04 VCALLSEE74 pKa = 4.68 TAGSSPSKK82 pKa = 10.03 LHH84 pKa = 6.56 DD85 pKa = 3.16 RR86 pKa = 11.84 HH87 pKa = 6.5 RR88 pKa = 11.84 PVCGEE93 pKa = 4.33 CCPACCSNVRR103 pKa = 11.84 SSSCEE108 pKa = 3.54 GKK110 pKa = 10.29 EE111 pKa = 3.64 ADD113 pKa = 3.54 LSKK116 pKa = 10.75 EE117 pKa = 3.77 RR118 pKa = 11.84 RR119 pKa = 11.84 LDD121 pKa = 3.48 SS122 pKa = 3.79
Molecular weight: 13.66 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.365
IPC2_protein 6.376
IPC_protein 6.517
Toseland 6.453
ProMoST 6.839
Dawson 7.015
Bjellqvist 6.942
Wikipedia 6.985
Rodwell 7.015
Grimsley 6.517
Solomon 7.044
Lehninger 7.059
Nozaki 7.307
DTASelect 7.322
Thurlkill 7.351
EMBOSS 7.366
Sillero 7.468
Patrickios 3.821
IPC_peptide 7.059
IPC2_peptide 7.176
IPC2.peptide.svr19 7.014
Protein with the highest isoelectric point:
>tr|A0A345GSF9|A0A345GSF9_9GEMI AC4 OS=Sweet potato leaf curl Hubei virus OX=2282485 PE=3 SV=1
MM1 pKa = 7.79 SDD3 pKa = 3.26 RR4 pKa = 11.84 LRR6 pKa = 11.84 VTRR9 pKa = 11.84 RR10 pKa = 11.84 YY11 pKa = 10.01 APYY14 pKa = 10.32 SRR16 pKa = 11.84 RR17 pKa = 11.84 PQAARR22 pKa = 11.84 RR23 pKa = 11.84 LNFTTDD29 pKa = 2.51 IVPYY33 pKa = 9.65 VGNAAPLAAATYY45 pKa = 10.31 VPVPVKK51 pKa = 10.37 ARR53 pKa = 11.84 KK54 pKa = 7.52 RR55 pKa = 11.84 TSRR58 pKa = 11.84 KK59 pKa = 9.25 RR60 pKa = 11.84 GDD62 pKa = 3.66 WIPRR66 pKa = 11.84 GCVGPCKK73 pKa = 10.08 VQDD76 pKa = 3.86 YY77 pKa = 8.72 EE78 pKa = 4.55 FKK80 pKa = 10.3 MDD82 pKa = 3.6 VPHH85 pKa = 7.17 GGTFVCVSDD94 pKa = 3.96 FTRR97 pKa = 11.84 GTGLTHH103 pKa = 7.28 RR104 pKa = 11.84 LGKK107 pKa = 9.86 RR108 pKa = 11.84 VCIKK112 pKa = 11.1 SMGFSGKK119 pKa = 7.94 VWMDD123 pKa = 3.53 DD124 pKa = 3.61 NIAKK128 pKa = 9.84 KK129 pKa = 10.52 DD130 pKa = 3.43 HH131 pKa = 6.37 TNIITFWLVRR141 pKa = 11.84 DD142 pKa = 3.76 RR143 pKa = 11.84 RR144 pKa = 11.84 PNKK147 pKa = 10.42 DD148 pKa = 3.21 PLTFSQLFHH157 pKa = 6.56 MFDD160 pKa = 4.05 NEE162 pKa = 3.9 PLTAKK167 pKa = 10.05 VRR169 pKa = 11.84 TDD171 pKa = 2.9 LRR173 pKa = 11.84 DD174 pKa = 3.17 RR175 pKa = 11.84 FQVLRR180 pKa = 11.84 TFSVTVSGGPYY191 pKa = 9.23 AHH193 pKa = 7.66 KK194 pKa = 10.09 EE195 pKa = 3.71 QAQVRR200 pKa = 11.84 RR201 pKa = 11.84 FFKK204 pKa = 10.71 GLNNHH209 pKa = 6.26 VIYY212 pKa = 10.49 NHH214 pKa = 6.53 KK215 pKa = 10.73 EE216 pKa = 3.41 EE217 pKa = 4.8 AKK219 pKa = 10.88 YY220 pKa = 10.75 EE221 pKa = 4.12 NQLEE225 pKa = 4.18 NAMLVYY231 pKa = 10.27 SASSHH236 pKa = 6.1 ASNPVYY242 pKa = 10.32 QSLRR246 pKa = 11.84 VRR248 pKa = 11.84 AYY250 pKa = 10.48 FYY252 pKa = 10.58 DD253 pKa = 3.16 SHH255 pKa = 6.48 MNN257 pKa = 3.42
Molecular weight: 29.7 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.29
IPC2_protein 9.575
IPC_protein 9.984
Toseland 10.218
ProMoST 9.94
Dawson 10.423
Bjellqvist 10.116
Wikipedia 10.613
Rodwell 10.73
Grimsley 10.496
Solomon 10.452
Lehninger 10.423
Nozaki 10.233
DTASelect 10.116
Thurlkill 10.277
EMBOSS 10.628
Sillero 10.335
Patrickios 10.306
IPC_peptide 10.452
IPC2_peptide 9.004
IPC2.peptide.svr19 8.596
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
1165
122
360
194.2
22.09
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.408 ± 0.805
2.661 ± 0.861
4.807 ± 0.412
4.979 ± 1.091
4.206 ± 0.581
5.236 ± 0.278
3.691 ± 0.346
5.15 ± 1.054
5.923 ± 0.395
8.24 ± 1.262
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.974 ± 0.608
4.549 ± 0.883
6.438 ± 0.748
4.378 ± 0.66
6.867 ± 1.138
9.871 ± 1.614
5.408 ± 0.851
5.15 ± 1.032
1.459 ± 0.214
3.605 ± 0.49
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here