Bacillus phage Gamma

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Wbetavirus; unclassified Wbetavirus

Average proteome isoelectric point is 6.56

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 53 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q3HKZ5|Q3HKZ5_9CAUD Putative phage regulatory protein OS=Bacillus phage Gamma OX=347962 GN=GAMMALSU_0024 PE=4 SV=1
MM1 pKa = 7.19NRR3 pKa = 11.84VNDD6 pKa = 3.8YY7 pKa = 11.02FGLEE11 pKa = 4.22SKK13 pKa = 10.61SDD15 pKa = 4.29CIWFYY20 pKa = 11.51GFFSISTILFLIDD33 pKa = 4.04MIIALII39 pKa = 3.93

Molecular weight: 4.6 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q3HKZ2|Q3HKZ2_9CAUD Site-specific recombinase OS=Bacillus phage Gamma OX=347962 GN=GAMMALSU_0027 PE=4 SV=1
MM1 pKa = 7.57IIVEE5 pKa = 4.46FKK7 pKa = 10.96DD8 pKa = 3.18RR9 pKa = 11.84LRR11 pKa = 11.84QLRR14 pKa = 11.84RR15 pKa = 11.84EE16 pKa = 4.16RR17 pKa = 11.84NLTQHH22 pKa = 7.28DD23 pKa = 4.44LGQAIGVTAGSITVTNNQLL42 pKa = 3.04

Molecular weight: 4.81 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

53

0

53

11221

39

1288

211.7

24.19

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.417 ± 0.583

0.775 ± 0.169

5.811 ± 0.256

8.894 ± 0.29

3.903 ± 0.205

5.917 ± 0.306

1.622 ± 0.229

6.916 ± 0.259

9.589 ± 0.391

8.448 ± 0.34

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.745 ± 0.161

5.383 ± 0.215

2.558 ± 0.252

4.171 ± 0.333

4.741 ± 0.225

5.713 ± 0.296

5.534 ± 0.308

6.176 ± 0.207

1.221 ± 0.175

3.467 ± 0.236
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski