Amino acid dipepetide frequency for Bacillus phage Gamma

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.991AlaAla: 4.991 ± 1.027
0.446AlaCys: 0.446 ± 0.184
3.387AlaAsp: 3.387 ± 0.746
5.704AlaGlu: 5.704 ± 0.807
3.209AlaPhe: 3.209 ± 0.396
4.456AlaGly: 4.456 ± 0.855
0.535AlaHis: 0.535 ± 0.207
5.348AlaIle: 5.348 ± 0.63
4.813AlaLys: 4.813 ± 0.734
5.437AlaLeu: 5.437 ± 0.722
1.426AlaMet: 1.426 ± 0.397
3.387AlaAsn: 3.387 ± 0.535
1.159AlaPro: 1.159 ± 0.279
2.139AlaGln: 2.139 ± 0.347
2.674AlaArg: 2.674 ± 0.458
4.011AlaSer: 4.011 ± 0.873
4.011AlaThr: 4.011 ± 0.763
4.189AlaVal: 4.189 ± 0.852
0.98AlaTrp: 0.98 ± 0.298
1.872AlaTyr: 1.872 ± 0.346
0.0AlaXaa: 0.0 ± 0.0
Cys
0.178CysAla: 0.178 ± 0.135
0.089CysCys: 0.089 ± 0.084
0.535CysAsp: 0.535 ± 0.151
0.713CysGlu: 0.713 ± 0.323
0.178CysPhe: 0.178 ± 0.115
0.624CysGly: 0.624 ± 0.197
0.178CysHis: 0.178 ± 0.121
0.713CysIle: 0.713 ± 0.231
0.535CysLys: 0.535 ± 0.2
0.535CysLeu: 0.535 ± 0.223
0.178CysMet: 0.178 ± 0.13
0.267CysAsn: 0.267 ± 0.146
0.713CysPro: 0.713 ± 0.295
0.357CysGln: 0.357 ± 0.167
0.446CysArg: 0.446 ± 0.191
0.357CysSer: 0.357 ± 0.186
0.267CysThr: 0.267 ± 0.13
0.357CysVal: 0.357 ± 0.152
0.0CysTrp: 0.0 ± 0.0
0.535CysTyr: 0.535 ± 0.257
0.0CysXaa: 0.0 ± 0.0
Asp
3.119AspAla: 3.119 ± 0.594
0.446AspCys: 0.446 ± 0.212
3.476AspAsp: 3.476 ± 0.538
5.348AspGlu: 5.348 ± 0.647
2.406AspPhe: 2.406 ± 0.469
4.1AspGly: 4.1 ± 0.504
0.713AspHis: 0.713 ± 0.215
3.654AspIle: 3.654 ± 0.527
6.506AspLys: 6.506 ± 0.517
5.08AspLeu: 5.08 ± 0.582
2.05AspMet: 2.05 ± 0.352
2.496AspAsn: 2.496 ± 0.555
1.426AspPro: 1.426 ± 0.419
2.05AspGln: 2.05 ± 0.372
2.941AspArg: 2.941 ± 0.499
2.406AspSer: 2.406 ± 0.428
2.763AspThr: 2.763 ± 0.48
4.278AspVal: 4.278 ± 0.515
0.802AspTrp: 0.802 ± 0.227
2.05AspTyr: 2.05 ± 0.584
0.0AspXaa: 0.0 ± 0.0
Glu
5.615GluAla: 5.615 ± 0.553
0.802GluCys: 0.802 ± 0.251
4.545GluAsp: 4.545 ± 0.523
8.645GluGlu: 8.645 ± 1.058
3.209GluPhe: 3.209 ± 0.471
4.278GluGly: 4.278 ± 0.543
1.426GluHis: 1.426 ± 0.325
7.932GluIle: 7.932 ± 0.636
8.378GluLys: 8.378 ± 0.927
8.734GluLeu: 8.734 ± 0.888
3.387GluMet: 3.387 ± 0.597
5.08GluAsn: 5.08 ± 0.522
1.159GluPro: 1.159 ± 0.375
4.456GluGln: 4.456 ± 0.8
4.189GluArg: 4.189 ± 0.629
4.189GluSer: 4.189 ± 0.771
4.367GluThr: 4.367 ± 0.695
4.902GluVal: 4.902 ± 0.63
0.891GluTrp: 0.891 ± 0.245
2.763GluTyr: 2.763 ± 0.445
0.0GluXaa: 0.0 ± 0.0
Phe
2.763PheAla: 2.763 ± 0.476
0.446PheCys: 0.446 ± 0.162
2.852PheAsp: 2.852 ± 0.542
2.585PheGlu: 2.585 ± 0.371
1.248PhePhe: 1.248 ± 0.307
2.496PheGly: 2.496 ± 0.434
0.713PheHis: 0.713 ± 0.223
1.872PheIle: 1.872 ± 0.483
3.654PheLys: 3.654 ± 0.425
3.922PheLeu: 3.922 ± 0.701
1.337PheMet: 1.337 ± 0.354
2.317PheAsn: 2.317 ± 0.387
0.891PhePro: 0.891 ± 0.275
1.248PheGln: 1.248 ± 0.339
2.139PheArg: 2.139 ± 0.449
2.139PheSer: 2.139 ± 0.504
2.317PheThr: 2.317 ± 0.529
2.05PheVal: 2.05 ± 0.392
0.446PheTrp: 0.446 ± 0.181
1.604PheTyr: 1.604 ± 0.385
0.0PheXaa: 0.0 ± 0.0
Gly
3.476GlyAla: 3.476 ± 0.564
0.357GlyCys: 0.357 ± 0.186
2.763GlyAsp: 2.763 ± 0.507
5.615GlyGlu: 5.615 ± 0.784
3.832GlyPhe: 3.832 ± 0.511
3.743GlyGly: 3.743 ± 0.622
1.159GlyHis: 1.159 ± 0.254
4.456GlyIle: 4.456 ± 0.659
6.061GlyLys: 6.061 ± 0.644
5.348GlyLeu: 5.348 ± 0.798
2.228GlyMet: 2.228 ± 0.66
2.763GlyAsn: 2.763 ± 0.551
0.624GlyPro: 0.624 ± 0.232
1.872GlyGln: 1.872 ± 0.341
2.228GlyArg: 2.228 ± 0.427
3.387GlySer: 3.387 ± 0.415
2.05GlyThr: 2.05 ± 0.45
3.922GlyVal: 3.922 ± 0.473
0.98GlyTrp: 0.98 ± 0.329
2.317GlyTyr: 2.317 ± 0.415
0.0GlyXaa: 0.0 ± 0.0
His
0.891HisAla: 0.891 ± 0.26
0.535HisCys: 0.535 ± 0.245
0.535HisAsp: 0.535 ± 0.217
1.337HisGlu: 1.337 ± 0.28
0.624HisPhe: 0.624 ± 0.244
0.535HisGly: 0.535 ± 0.19
0.535HisHis: 0.535 ± 0.241
0.98HisIle: 0.98 ± 0.271
1.248HisLys: 1.248 ± 0.286
1.159HisLeu: 1.159 ± 0.245
0.802HisMet: 0.802 ± 0.275
0.891HisAsn: 0.891 ± 0.293
0.535HisPro: 0.535 ± 0.257
0.535HisGln: 0.535 ± 0.191
0.98HisArg: 0.98 ± 0.217
0.713HisSer: 0.713 ± 0.366
1.07HisThr: 1.07 ± 0.407
0.624HisVal: 0.624 ± 0.258
0.357HisTrp: 0.357 ± 0.145
1.337HisTyr: 1.337 ± 0.363
0.0HisXaa: 0.0 ± 0.0
Ile
4.991IleAla: 4.991 ± 0.506
0.624IleCys: 0.624 ± 0.205
5.348IleAsp: 5.348 ± 0.721
6.506IleGlu: 6.506 ± 0.653
1.693IlePhe: 1.693 ± 0.422
3.476IleGly: 3.476 ± 0.632
1.07IleHis: 1.07 ± 0.313
2.941IleIle: 2.941 ± 0.456
6.774IleLys: 6.774 ± 0.809
5.526IleLeu: 5.526 ± 0.741
1.248IleMet: 1.248 ± 0.316
4.011IleAsn: 4.011 ± 0.565
2.496IlePro: 2.496 ± 0.508
3.298IleGln: 3.298 ± 0.645
3.922IleArg: 3.922 ± 0.45
4.724IleSer: 4.724 ± 0.539
3.565IleThr: 3.565 ± 0.51
4.189IleVal: 4.189 ± 0.551
0.98IleTrp: 0.98 ± 0.251
1.783IleTyr: 1.783 ± 0.469
0.0IleXaa: 0.0 ± 0.0
Lys
6.15LysAla: 6.15 ± 0.876
0.624LysCys: 0.624 ± 0.173
3.832LysAsp: 3.832 ± 0.512
9.804LysGlu: 9.804 ± 1.136
2.941LysPhe: 2.941 ± 0.413
5.882LysGly: 5.882 ± 0.685
1.961LysHis: 1.961 ± 0.474
7.041LysIle: 7.041 ± 0.838
9.626LysLys: 9.626 ± 0.963
8.913LysLeu: 8.913 ± 0.941
3.03LysMet: 3.03 ± 0.499
5.08LysAsn: 5.08 ± 0.728
2.585LysPro: 2.585 ± 0.533
3.119LysGln: 3.119 ± 0.496
4.278LysArg: 4.278 ± 0.612
4.902LysSer: 4.902 ± 0.617
5.08LysThr: 5.08 ± 0.755
6.863LysVal: 6.863 ± 0.6
1.159LysTrp: 1.159 ± 0.276
2.941LysTyr: 2.941 ± 0.563
0.0LysXaa: 0.0 ± 0.0
Leu
4.813LeuAla: 4.813 ± 0.759
0.624LeuCys: 0.624 ± 0.307
5.258LeuAsp: 5.258 ± 0.647
6.952LeuGlu: 6.952 ± 0.692
3.119LeuPhe: 3.119 ± 0.576
4.991LeuGly: 4.991 ± 0.497
1.07LeuHis: 1.07 ± 0.305
4.635LeuIle: 4.635 ± 0.612
9.002LeuLys: 9.002 ± 0.793
6.506LeuLeu: 6.506 ± 0.881
1.961LeuMet: 1.961 ± 0.357
5.526LeuAsn: 5.526 ± 0.757
1.961LeuPro: 1.961 ± 0.412
4.278LeuGln: 4.278 ± 0.773
3.654LeuArg: 3.654 ± 0.66
5.437LeuSer: 5.437 ± 0.586
5.615LeuThr: 5.615 ± 0.491
4.813LeuVal: 4.813 ± 0.623
0.891LeuTrp: 0.891 ± 0.297
3.387LeuTyr: 3.387 ± 0.713
0.0LeuXaa: 0.0 ± 0.0
Met
1.515MetAla: 1.515 ± 0.406
0.089MetCys: 0.089 ± 0.09
1.961MetAsp: 1.961 ± 0.439
1.783MetGlu: 1.783 ± 0.264
0.713MetPhe: 0.713 ± 0.385
1.515MetGly: 1.515 ± 0.408
0.446MetHis: 0.446 ± 0.232
2.05MetIle: 2.05 ± 0.375
3.387MetLys: 3.387 ± 0.561
1.426MetLeu: 1.426 ± 0.25
0.535MetMet: 0.535 ± 0.189
1.515MetAsn: 1.515 ± 0.446
0.891MetPro: 0.891 ± 0.29
1.07MetGln: 1.07 ± 0.301
1.693MetArg: 1.693 ± 0.312
1.872MetSer: 1.872 ± 0.348
2.228MetThr: 2.228 ± 0.393
1.248MetVal: 1.248 ± 0.349
0.713MetTrp: 0.713 ± 0.216
0.802MetTyr: 0.802 ± 0.326
0.0MetXaa: 0.0 ± 0.0
Asn
4.011AsnAla: 4.011 ± 0.531
0.089AsnCys: 0.089 ± 0.089
2.674AsnAsp: 2.674 ± 0.322
5.704AsnGlu: 5.704 ± 0.685
1.961AsnPhe: 1.961 ± 0.438
4.011AsnGly: 4.011 ± 0.529
1.248AsnHis: 1.248 ± 0.426
3.209AsnIle: 3.209 ± 0.467
4.902AsnLys: 4.902 ± 0.533
3.298AsnLeu: 3.298 ± 0.481
1.515AsnMet: 1.515 ± 0.371
2.05AsnAsn: 2.05 ± 0.349
1.872AsnPro: 1.872 ± 0.414
2.763AsnGln: 2.763 ± 0.596
4.189AsnArg: 4.189 ± 0.602
2.585AsnSer: 2.585 ± 0.515
2.585AsnThr: 2.585 ± 0.557
3.209AsnVal: 3.209 ± 0.527
0.802AsnTrp: 0.802 ± 0.304
1.159AsnTyr: 1.159 ± 0.363
0.0AsnXaa: 0.0 ± 0.0
Pro
1.604ProAla: 1.604 ± 0.325
0.267ProCys: 0.267 ± 0.198
1.159ProAsp: 1.159 ± 0.329
2.406ProGlu: 2.406 ± 0.485
0.624ProPhe: 0.624 ± 0.181
1.159ProGly: 1.159 ± 0.191
0.713ProHis: 0.713 ± 0.298
1.872ProIle: 1.872 ± 0.519
1.604ProLys: 1.604 ± 0.414
2.05ProLeu: 2.05 ± 0.47
0.713ProMet: 0.713 ± 0.254
1.337ProAsn: 1.337 ± 0.296
0.624ProPro: 0.624 ± 0.206
1.159ProGln: 1.159 ± 0.295
0.891ProArg: 0.891 ± 0.354
2.496ProSer: 2.496 ± 0.36
1.693ProThr: 1.693 ± 0.404
1.872ProVal: 1.872 ± 0.386
0.267ProTrp: 0.267 ± 0.234
1.07ProTyr: 1.07 ± 0.313
0.0ProXaa: 0.0 ± 0.0
Gln
3.119GlnAla: 3.119 ± 0.817
0.178GlnCys: 0.178 ± 0.147
1.961GlnAsp: 1.961 ± 0.408
3.298GlnGlu: 3.298 ± 0.475
1.872GlnPhe: 1.872 ± 0.408
2.139GlnGly: 2.139 ± 0.394
0.802GlnHis: 0.802 ± 0.24
2.496GlnIle: 2.496 ± 0.382
4.456GlnLys: 4.456 ± 0.753
2.585GlnLeu: 2.585 ± 0.514
1.426GlnMet: 1.426 ± 0.36
2.496GlnAsn: 2.496 ± 0.633
1.693GlnPro: 1.693 ± 0.312
2.585GlnGln: 2.585 ± 0.648
1.961GlnArg: 1.961 ± 0.409
2.852GlnSer: 2.852 ± 0.459
1.604GlnThr: 1.604 ± 0.353
2.496GlnVal: 2.496 ± 0.428
0.089GlnTrp: 0.089 ± 0.1
1.604GlnTyr: 1.604 ± 0.336
0.0GlnXaa: 0.0 ± 0.0
Arg
3.03ArgAla: 3.03 ± 0.448
0.357ArgCys: 0.357 ± 0.171
3.387ArgAsp: 3.387 ± 0.51
4.545ArgGlu: 4.545 ± 0.481
1.961ArgPhe: 1.961 ± 0.363
2.763ArgGly: 2.763 ± 0.598
0.624ArgHis: 0.624 ± 0.278
3.387ArgIle: 3.387 ± 0.633
4.011ArgLys: 4.011 ± 0.767
4.545ArgLeu: 4.545 ± 0.556
0.98ArgMet: 0.98 ± 0.262
2.585ArgAsn: 2.585 ± 0.671
0.98ArgPro: 0.98 ± 0.309
2.317ArgGln: 2.317 ± 0.485
1.783ArgArg: 1.783 ± 0.417
2.941ArgSer: 2.941 ± 0.566
1.961ArgThr: 1.961 ± 0.326
2.585ArgVal: 2.585 ± 0.403
0.624ArgTrp: 0.624 ± 0.224
2.05ArgTyr: 2.05 ± 0.473
0.0ArgXaa: 0.0 ± 0.0
Ser
3.387SerAla: 3.387 ± 0.825
0.267SerCys: 0.267 ± 0.156
4.011SerAsp: 4.011 ± 0.464
3.387SerGlu: 3.387 ± 0.587
2.139SerPhe: 2.139 ± 0.592
3.832SerGly: 3.832 ± 0.574
1.159SerHis: 1.159 ± 0.327
3.832SerIle: 3.832 ± 0.532
6.595SerLys: 6.595 ± 0.845
5.882SerLeu: 5.882 ± 0.67
1.515SerMet: 1.515 ± 0.382
3.298SerAsn: 3.298 ± 0.63
1.248SerPro: 1.248 ± 0.402
2.05SerGln: 2.05 ± 0.412
2.05SerArg: 2.05 ± 0.414
2.941SerSer: 2.941 ± 0.551
3.565SerThr: 3.565 ± 0.555
3.832SerVal: 3.832 ± 0.505
0.624SerTrp: 0.624 ± 0.309
1.515SerTyr: 1.515 ± 0.371
0.0SerXaa: 0.0 ± 0.0
Thr
3.922ThrAla: 3.922 ± 0.715
0.089ThrCys: 0.089 ± 0.085
3.209ThrAsp: 3.209 ± 0.463
5.08ThrGlu: 5.08 ± 0.869
2.496ThrPhe: 2.496 ± 0.456
3.476ThrGly: 3.476 ± 0.628
0.624ThrHis: 0.624 ± 0.281
4.813ThrIle: 4.813 ± 0.639
4.724ThrLys: 4.724 ± 0.572
4.813ThrLeu: 4.813 ± 0.593
1.159ThrMet: 1.159 ± 0.285
2.406ThrAsn: 2.406 ± 0.478
2.05ThrPro: 2.05 ± 0.524
2.406ThrGln: 2.406 ± 0.438
2.05ThrArg: 2.05 ± 0.357
2.763ThrSer: 2.763 ± 0.446
3.743ThrThr: 3.743 ± 0.61
3.654ThrVal: 3.654 ± 0.466
0.0ThrTrp: 0.0 ± 0.0
1.872ThrTyr: 1.872 ± 0.349
0.0ThrXaa: 0.0 ± 0.0
Val
3.654ValAla: 3.654 ± 0.627
0.535ValCys: 0.535 ± 0.29
4.545ValAsp: 4.545 ± 0.496
5.526ValGlu: 5.526 ± 0.842
2.228ValPhe: 2.228 ± 0.505
3.03ValGly: 3.03 ± 0.468
0.624ValHis: 0.624 ± 0.249
4.635ValIle: 4.635 ± 0.576
4.991ValLys: 4.991 ± 0.574
4.724ValLeu: 4.724 ± 0.877
0.98ValMet: 0.98 ± 0.302
3.654ValAsn: 3.654 ± 0.694
1.783ValPro: 1.783 ± 0.375
2.674ValGln: 2.674 ± 0.496
3.119ValArg: 3.119 ± 0.416
4.011ValSer: 4.011 ± 0.603
3.922ValThr: 3.922 ± 0.567
3.654ValVal: 3.654 ± 0.553
0.713ValTrp: 0.713 ± 0.271
2.763ValTyr: 2.763 ± 0.605
0.0ValXaa: 0.0 ± 0.0
Trp
0.624TrpAla: 0.624 ± 0.317
0.178TrpCys: 0.178 ± 0.123
0.802TrpAsp: 0.802 ± 0.242
0.98TrpGlu: 0.98 ± 0.35
0.624TrpPhe: 0.624 ± 0.251
0.624TrpGly: 0.624 ± 0.259
0.178TrpHis: 0.178 ± 0.101
1.07TrpIle: 1.07 ± 0.393
0.713TrpLys: 0.713 ± 0.268
1.159TrpLeu: 1.159 ± 0.36
0.178TrpMet: 0.178 ± 0.114
0.713TrpAsn: 0.713 ± 0.211
0.089TrpPro: 0.089 ± 0.084
0.446TrpGln: 0.446 ± 0.192
0.535TrpArg: 0.535 ± 0.187
1.07TrpSer: 1.07 ± 0.295
0.802TrpThr: 0.802 ± 0.261
0.713TrpVal: 0.713 ± 0.246
0.267TrpTrp: 0.267 ± 0.177
0.446TrpTyr: 0.446 ± 0.162
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.317TyrAla: 2.317 ± 0.501
0.713TyrCys: 0.713 ± 0.301
1.872TyrAsp: 1.872 ± 0.469
3.03TyrGlu: 3.03 ± 0.482
1.961TyrPhe: 1.961 ± 0.331
2.05TyrGly: 2.05 ± 0.377
0.446TyrHis: 0.446 ± 0.176
2.228TyrIle: 2.228 ± 0.432
3.922TyrLys: 3.922 ± 0.732
2.852TyrLeu: 2.852 ± 0.74
0.713TyrMet: 0.713 ± 0.173
1.961TyrAsn: 1.961 ± 0.436
0.802TyrPro: 0.802 ± 0.27
0.891TyrGln: 0.891 ± 0.299
1.693TyrArg: 1.693 ± 0.429
1.337TyrSer: 1.337 ± 0.229
2.139TyrThr: 2.139 ± 0.322
2.317TyrVal: 2.317 ± 0.396
0.624TyrTrp: 0.624 ± 0.219
0.802TyrTyr: 0.802 ± 0.262
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 53 proteins (11221 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski