Beggiatoa sp. SS
Average proteome isoelectric point is 6.92
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1437 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A7BL97|A7BL97_9GAMM Uncharacterized protein OS=Beggiatoa sp. SS OX=422288 GN=BGS_0189 PE=4 SV=1
MM1 pKa = 8.1 PDD3 pKa = 3.22 GTSVSLFTKK12 pKa = 10.34 GGTIGQSIGGGAFTTTTEE30 pKa = 4.21 LGRR33 pKa = 11.84 ATAILQSANPSTPLLGGVATYY54 pKa = 10.86 RR55 pKa = 11.84 EE56 pKa = 4.0 VGYY59 pKa = 10.62 DD60 pKa = 3.2 CSGNYY65 pKa = 10.51 AFVTSPGTPLLLCSNPGLVTIVAFTTGSEE94 pKa = 4.25 SFVDD98 pKa = 3.55 KK99 pKa = 11.12 NGNGLYY105 pKa = 10.63 DD106 pKa = 4.6 ADD108 pKa = 5.8 DD109 pKa = 4.32 SFTDD113 pKa = 2.97 ICEE116 pKa = 4.03 PFIDD120 pKa = 5.15 GNDD123 pKa = 3.18 NGQFDD128 pKa = 4.21 EE129 pKa = 5.06 GEE131 pKa = 4.23 LYY133 pKa = 10.23 IDD135 pKa = 4.01 VNSNGAFDD143 pKa = 3.72 TGNGQFDD150 pKa = 4.45 GPGGPSEE157 pKa = 4.55 NTTIWYY163 pKa = 6.47 STRR166 pKa = 11.84 VLFSDD171 pKa = 3.98 RR172 pKa = 11.84 TAPIQVKK179 pKa = 8.84 PASFSIPNGGSEE191 pKa = 4.26 TFTIQNISDD200 pKa = 3.47 IYY202 pKa = 11.28 GNALVKK208 pKa = 10.72 NSQFNVTTNNGKK220 pKa = 10.18 LGGVTHH226 pKa = 6.31 FTFGDD231 pKa = 4.04 SNTPAQSGIQFTLSSNPCSNSVTNEE256 pKa = 4.0 DD257 pKa = 3.39 GSVTEE262 pKa = 4.63 KK263 pKa = 10.89 CPPPP267 pKa = 4.78
Molecular weight: 27.76 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.73
IPC2_protein 3.91
IPC_protein 3.897
Toseland 3.681
ProMoST 4.062
Dawson 3.884
Bjellqvist 4.037
Wikipedia 3.821
Rodwell 3.719
Grimsley 3.592
Solomon 3.872
Lehninger 3.834
Nozaki 3.999
DTASelect 4.24
Thurlkill 3.732
EMBOSS 3.834
Sillero 4.012
Patrickios 1.074
IPC_peptide 3.872
IPC2_peptide 3.986
IPC2.peptide.svr19 3.891
Protein with the highest isoelectric point:
>tr|A7BMI9|A7BMI9_9GAMM Extracellular ligand-binding receptor OS=Beggiatoa sp. SS OX=422288 GN=BGS_0341 PE=4 SV=1
MM1 pKa = 7.57 ASVSISCPSCSATDD15 pKa = 3.7 GVVRR19 pKa = 11.84 NGKK22 pKa = 8.27 STAGHH27 pKa = 4.88 QRR29 pKa = 11.84 YY30 pKa = 8.94 LCSHH34 pKa = 6.46 CRR36 pKa = 11.84 KK37 pKa = 7.49 TWQLQFTYY45 pKa = 9.73 TASQPGTHH53 pKa = 5.79 QKK55 pKa = 10.33 IIDD58 pKa = 3.52 MAMNGVGCRR67 pKa = 11.84 ATARR71 pKa = 11.84 IMGVGLNTILRR82 pKa = 11.84 HH83 pKa = 5.55 LKK85 pKa = 10.06 NSGRR89 pKa = 11.84 SRR91 pKa = 3.37
Molecular weight: 9.87 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.353
IPC2_protein 9.545
IPC_protein 10.087
Toseland 10.467
ProMoST 10.452
Dawson 10.599
Bjellqvist 10.321
Wikipedia 10.774
Rodwell 10.877
Grimsley 10.643
Solomon 10.672
Lehninger 10.657
Nozaki 10.555
DTASelect 10.277
Thurlkill 10.482
EMBOSS 10.862
Sillero 10.54
Patrickios 10.672
IPC_peptide 10.672
IPC2_peptide 9.765
IPC2.peptide.svr19 8.547
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1437
0
1437
212427
35
1482
147.8
16.55
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.347 ± 0.085
1.232 ± 0.036
4.96 ± 0.107
5.858 ± 0.086
4.458 ± 0.073
6.729 ± 0.099
2.472 ± 0.043
6.575 ± 0.068
5.509 ± 0.074
10.562 ± 0.114
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.439 ± 0.047
4.503 ± 0.064
4.933 ± 0.073
4.904 ± 0.078
4.928 ± 0.069
6.432 ± 0.078
5.731 ± 0.086
5.982 ± 0.066
1.277 ± 0.032
3.077 ± 0.048
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here