Prevotella corporis
Average proteome isoelectric point is 6.99
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2956 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A133PSX3|A0A133PSX3_9BACT YqeY-like protein OS=Prevotella corporis OX=28128 GN=HMPREF3226_02801 PE=4 SV=1
MM1 pKa = 7.23 TKK3 pKa = 10.1 VFVMSTCPDD12 pKa = 3.51 CTQVKK17 pKa = 9.72 AQLAGNPNYY26 pKa = 10.32 EE27 pKa = 4.5 LIDD30 pKa = 3.5 IGEE33 pKa = 4.24 HH34 pKa = 5.26 VRR36 pKa = 11.84 NLKK39 pKa = 10.12 QFLTLRR45 pKa = 11.84 DD46 pKa = 3.99 SNPAFDD52 pKa = 4.6 DD53 pKa = 3.78 ARR55 pKa = 11.84 AHH57 pKa = 6.37 GYY59 pKa = 10.07 VGIPCFVLDD68 pKa = 4.85 DD69 pKa = 4.16 GTVSFSIDD77 pKa = 3.51 DD78 pKa = 3.83 VVLEE82 pKa = 4.56 DD83 pKa = 3.84 TPEE86 pKa = 4.17 GASCNLDD93 pKa = 3.25 GTGCC97 pKa = 3.64
Molecular weight: 10.48 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.854
IPC2_protein 4.126
IPC_protein 4.075
Toseland 3.859
ProMoST 4.253
Dawson 4.075
Bjellqvist 4.228
Wikipedia 4.037
Rodwell 3.897
Grimsley 3.77
Solomon 4.062
Lehninger 4.024
Nozaki 4.202
DTASelect 4.457
Thurlkill 3.923
EMBOSS 4.037
Sillero 4.202
Patrickios 1.977
IPC_peptide 4.062
IPC2_peptide 4.177
IPC2.peptide.svr19 4.087
Protein with the highest isoelectric point:
>tr|A0A133PW65|A0A133PW65_9BACT Uncharacterized protein OS=Prevotella corporis OX=28128 GN=HMPREF3226_02334 PE=4 SV=1
MM1 pKa = 7.35 NGHH4 pKa = 6.53 MPNCQHH10 pKa = 5.37 ITARR14 pKa = 11.84 HH15 pKa = 4.5 LTAIIAALLLLVMEE29 pKa = 5.69 GCTSSRR35 pKa = 11.84 FGEE38 pKa = 4.69 GGHH41 pKa = 6.21 TFRR44 pKa = 11.84 QDD46 pKa = 2.83 NPEE49 pKa = 3.38 IDD51 pKa = 3.57 RR52 pKa = 11.84 RR53 pKa = 11.84 FQIADD58 pKa = 3.38 LGGQQLEE65 pKa = 3.98 AATRR69 pKa = 11.84 YY70 pKa = 9.97 GLSKK74 pKa = 10.17 PIRR77 pKa = 11.84 RR78 pKa = 11.84 RR79 pKa = 11.84 SGIWLRR85 pKa = 11.84 AWRR88 pKa = 11.84 LRR90 pKa = 11.84 KK91 pKa = 9.49 IKK93 pKa = 9.18 TNRR96 pKa = 11.84 YY97 pKa = 8.44 YY98 pKa = 11.26 LLDD101 pKa = 4.33 PMPHH105 pKa = 6.2 SNPYY109 pKa = 8.14 LTSGGKK115 pKa = 8.42 WVLKK119 pKa = 9.53 TIAKK123 pKa = 9.94 RR124 pKa = 11.84 FQKK127 pKa = 10.44 KK128 pKa = 8.5 LRR130 pKa = 11.84 KK131 pKa = 9.34 AGYY134 pKa = 8.0 RR135 pKa = 11.84 EE136 pKa = 3.54 HH137 pKa = 7.47 RR138 pKa = 11.84 IIVTSMLRR146 pKa = 11.84 TRR148 pKa = 11.84 EE149 pKa = 4.23 DD150 pKa = 2.68 IGGLKK155 pKa = 10.11 KK156 pKa = 10.28 INRR159 pKa = 11.84 VAATNSSHH167 pKa = 6.78 MYY169 pKa = 8.37 GTTFDD174 pKa = 3.5 VSFTRR179 pKa = 11.84 FNRR182 pKa = 11.84 ISMDD186 pKa = 2.72 GMQPSNRR193 pKa = 11.84 LMANLLGEE201 pKa = 4.62 VICEE205 pKa = 3.8 LRR207 pKa = 11.84 EE208 pKa = 4.0 EE209 pKa = 4.81 GEE211 pKa = 3.93 CWIIFEE217 pKa = 4.55 RR218 pKa = 11.84 SQHH221 pKa = 5.68 CFHH224 pKa = 6.02 VTARR228 pKa = 11.84 RR229 pKa = 3.41
Molecular weight: 26.49 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.353
IPC2_protein 9.619
IPC_protein 10.409
Toseland 10.613
ProMoST 10.438
Dawson 10.716
Bjellqvist 10.438
Wikipedia 10.921
Rodwell 10.906
Grimsley 10.774
Solomon 10.818
Lehninger 10.789
Nozaki 10.613
DTASelect 10.423
Thurlkill 10.613
EMBOSS 11.008
Sillero 10.657
Patrickios 10.613
IPC_peptide 10.833
IPC2_peptide 9.56
IPC2.peptide.svr19 8.63
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2956
0
2956
835971
23
2529
282.8
32.02
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.742 ± 0.042
1.276 ± 0.018
5.607 ± 0.034
6.348 ± 0.046
4.621 ± 0.031
6.526 ± 0.043
2.038 ± 0.019
7.209 ± 0.046
7.196 ± 0.04
8.881 ± 0.049
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.856 ± 0.024
5.352 ± 0.042
3.52 ± 0.023
3.652 ± 0.026
4.79 ± 0.04
6.157 ± 0.034
5.6 ± 0.039
6.277 ± 0.035
1.1 ± 0.017
4.251 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here