Changuinola virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Duplornaviricota; Resentoviricetes; Reovirales; Reoviridae; Sedoreovirinae; Orbivirus

Average proteome isoelectric point is 6.49

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|U5YL69|U5YL69_9REOV Core protein VP7 OS=Changuinola virus OX=40052 GN=CGLV_VP7 PE=3 SV=1
MM1 pKa = 7.4EE2 pKa = 5.5SKK4 pKa = 10.37PKK6 pKa = 10.28RR7 pKa = 11.84VFTKK11 pKa = 8.18TVCVLDD17 pKa = 4.6AYY19 pKa = 10.83GKK21 pKa = 6.63TFCGNVAKK29 pKa = 10.63LCNKK33 pKa = 8.62PYY35 pKa = 10.66CLIKK39 pKa = 9.96IGRR42 pKa = 11.84VVSIEE47 pKa = 4.18AVDD50 pKa = 3.65NPRR53 pKa = 11.84PKK55 pKa = 10.57SYY57 pKa = 10.44ILQIEE62 pKa = 4.22QVGSYY67 pKa = 10.0RR68 pKa = 11.84IQDD71 pKa = 3.65GQDD74 pKa = 3.43NISLIITNQGVEE86 pKa = 4.13ATVEE90 pKa = 4.02RR91 pKa = 11.84WEE93 pKa = 3.91EE94 pKa = 3.75WKK96 pKa = 10.86FEE98 pKa = 4.13NLSPIPMATMIMVADD113 pKa = 3.74KK114 pKa = 10.98RR115 pKa = 11.84VDD117 pKa = 3.43AEE119 pKa = 4.47VKK121 pKa = 9.58FVKK124 pKa = 10.48GMGIIAPYY132 pKa = 8.86TKK134 pKa = 10.77NEE136 pKa = 3.67MDD138 pKa = 3.65RR139 pKa = 11.84RR140 pKa = 11.84DD141 pKa = 3.76VPDD144 pKa = 4.18LPGVMPSSMGVKK156 pKa = 9.74EE157 pKa = 4.11LRR159 pKa = 11.84EE160 pKa = 3.69KK161 pKa = 10.81LRR163 pKa = 11.84NEE165 pKa = 3.89RR166 pKa = 11.84EE167 pKa = 3.78QGKK170 pKa = 9.27VRR172 pKa = 11.84EE173 pKa = 4.27SEE175 pKa = 3.93IVRR178 pKa = 11.84PAVVNHH184 pKa = 5.65VVEE187 pKa = 4.23QPKK190 pKa = 9.28WEE192 pKa = 4.48DD193 pKa = 3.52LDD195 pKa = 3.92TKK197 pKa = 10.52IAAVTDD203 pKa = 3.82WNADD207 pKa = 3.47VPSEE211 pKa = 4.13IPHH214 pKa = 6.21GSSEE218 pKa = 4.14EE219 pKa = 3.99EE220 pKa = 4.0EE221 pKa = 4.56EE222 pKa = 4.31EE223 pKa = 4.17GGEE226 pKa = 4.17YY227 pKa = 9.74LTGKK231 pKa = 9.69YY232 pKa = 7.73MKK234 pKa = 10.04KK235 pKa = 10.24VSTIVKK241 pKa = 10.05NPDD244 pKa = 3.21PSVLGMAFGYY254 pKa = 9.22PKK256 pKa = 10.32TSGPFDD262 pKa = 4.79RR263 pKa = 11.84IIVEE267 pKa = 5.15KK268 pKa = 9.9EE269 pKa = 4.11CLWTGVPIYY278 pKa = 10.87DD279 pKa = 4.01VDD281 pKa = 3.96QTDD284 pKa = 2.62KK285 pKa = 11.12SYY287 pKa = 10.65RR288 pKa = 11.84LVMIGEE294 pKa = 4.07ATKK297 pKa = 11.08YY298 pKa = 10.19LLAVKK303 pKa = 9.27GQNYY307 pKa = 8.55FVLPAGAGSAA317 pKa = 3.72

Molecular weight: 35.51 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|U5NU19|U5NU19_9REOV Outer capsid protein VP2 OS=Changuinola virus OX=40052 GN=CGLV_VP2 PE=3 SV=1
MM1 pKa = 7.56LSEE4 pKa = 3.89LAARR8 pKa = 11.84FEE10 pKa = 4.36AGKK13 pKa = 9.84QRR15 pKa = 11.84EE16 pKa = 4.23LEE18 pKa = 4.15EE19 pKa = 4.41VNMLNEE25 pKa = 4.26TAVVPYY31 pKa = 8.85TRR33 pKa = 11.84PPSYY37 pKa = 11.08APTAPTTFAPAHH49 pKa = 6.18LSLNILNNAMSNGTAKK65 pKa = 10.05TNALKK70 pKa = 10.5EE71 pKa = 4.18EE72 pKa = 4.19KK73 pKa = 10.36VAFGSYY79 pKa = 10.82AEE81 pKa = 4.35AFRR84 pKa = 11.84DD85 pKa = 4.09EE86 pKa = 4.72PAVQQIKK93 pKa = 9.82SHH95 pKa = 5.52VNEE98 pKa = 4.05QIIPKK103 pKa = 10.06LKK105 pKa = 10.55RR106 pKa = 11.84EE107 pKa = 3.69LAGYY111 pKa = 8.88KK112 pKa = 9.4KK113 pKa = 10.45KK114 pKa = 10.45RR115 pKa = 11.84WLVHH119 pKa = 4.87LTMLIAAGVALFTSLGTLVKK139 pKa = 10.19DD140 pKa = 3.53VQIRR144 pKa = 11.84IPGNNSTAGANEE156 pKa = 4.57YY157 pKa = 10.17IHH159 pKa = 7.01LPTWYY164 pKa = 8.69TSLGAIFGIVNLGATGLMISCARR187 pKa = 11.84IEE189 pKa = 3.96KK190 pKa = 10.62SYY192 pKa = 11.43DD193 pKa = 3.08EE194 pKa = 5.34SIAFLKK200 pKa = 10.48KK201 pKa = 10.15EE202 pKa = 3.94LMKK205 pKa = 10.69KK206 pKa = 10.44RR207 pKa = 11.84SYY209 pKa = 11.42NDD211 pKa = 4.13AIRR214 pKa = 11.84MSIKK218 pKa = 10.89DD219 pKa = 3.62MGSLTNLFSEE229 pKa = 4.89EE230 pKa = 3.8QHH232 pKa = 5.35QQ233 pKa = 3.54

Molecular weight: 25.82 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10

0

10

6321

233

1309

632.1

72.43

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.138 ± 0.66

1.155 ± 0.281

5.759 ± 0.332

7.293 ± 0.512

4.018 ± 0.354

5.885 ± 0.396

2.421 ± 0.233

7.087 ± 0.326

6.186 ± 0.707

8.448 ± 0.321

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.654 ± 0.399

4.888 ± 0.271

3.734 ± 0.347

3.623 ± 0.277

6.423 ± 0.496

5.474 ± 0.492

5.363 ± 0.398

7.182 ± 0.326

1.202 ± 0.181

4.066 ± 0.344
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski