Circovirus-like genome DCCV-3

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Arfiviricetes; Cirlivirales; Circoviridae; unclassified Circoviridae

Average proteome isoelectric point is 7.06

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A190WHA2|A0A190WHA2_9CIRC Uncharacterized protein OS=Circovirus-like genome DCCV-3 OX=1788443 PE=4 SV=1
MM1 pKa = 6.07TTNYY5 pKa = 9.58YY6 pKa = 10.11RR7 pKa = 11.84PKK9 pKa = 10.11NCWKK13 pKa = 9.41QPHH16 pKa = 5.65MEE18 pKa = 4.15YY19 pKa = 10.18TEE21 pKa = 4.32SRR23 pKa = 11.84SEE25 pKa = 4.21TVEE28 pKa = 3.77HH29 pKa = 6.7NIYY32 pKa = 10.5RR33 pKa = 11.84DD34 pKa = 3.34MDD36 pKa = 3.41IGQIKK41 pKa = 10.37SPSLQSKK48 pKa = 9.67RR49 pKa = 11.84LSPEE53 pKa = 4.13HH54 pKa = 6.48ISNVQRR60 pKa = 11.84EE61 pKa = 4.36MPEE64 pKa = 3.77RR65 pKa = 11.84TNDD68 pKa = 3.05IVLKK72 pKa = 9.72TEE74 pKa = 3.56ITQNMEE80 pKa = 3.95YY81 pKa = 10.46YY82 pKa = 10.32HH83 pKa = 7.37NEE85 pKa = 3.69VDD87 pKa = 4.96EE88 pKa = 4.45TTLWHH93 pKa = 6.49SKK95 pKa = 9.38TIYY98 pKa = 10.24SQVV101 pKa = 2.91

Molecular weight: 12.21 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A190WHB4|A0A190WHB4_9CIRC ATP-dependent helicase Rep OS=Circovirus-like genome DCCV-3 OX=1788443 PE=3 SV=1
MM1 pKa = 7.14FRR3 pKa = 11.84RR4 pKa = 11.84RR5 pKa = 11.84NRR7 pKa = 11.84THH9 pKa = 6.24RR10 pKa = 11.84RR11 pKa = 11.84KK12 pKa = 9.51PRR14 pKa = 11.84RR15 pKa = 11.84SARR18 pKa = 11.84RR19 pKa = 11.84KK20 pKa = 8.95KK21 pKa = 9.0PLRR24 pKa = 11.84RR25 pKa = 11.84IMRR28 pKa = 11.84PSMRR32 pKa = 11.84MIRR35 pKa = 11.84SPIPEE40 pKa = 4.4RR41 pKa = 11.84IFVKK45 pKa = 10.41LRR47 pKa = 11.84YY48 pKa = 9.41SEE50 pKa = 4.43ISTVTCPVAYY60 pKa = 9.78QLYY63 pKa = 9.48QFATYY68 pKa = 10.22SSSVYY73 pKa = 10.48DD74 pKa = 4.02PNKK77 pKa = 10.45SGIGHH82 pKa = 5.19QPMWRR87 pKa = 11.84DD88 pKa = 3.49QYY90 pKa = 10.03ATLYY94 pKa = 9.66QEE96 pKa = 3.79YY97 pKa = 9.22RR98 pKa = 11.84VRR100 pKa = 11.84GFTYY104 pKa = 10.04RR105 pKa = 11.84VQAANRR111 pKa = 11.84NANEE115 pKa = 4.23AGWFGVNHH123 pKa = 6.61SSSQVITDD131 pKa = 3.16TDD133 pKa = 3.37IQNWCEE139 pKa = 3.47RR140 pKa = 11.84RR141 pKa = 11.84TAKK144 pKa = 10.15VRR146 pKa = 11.84MLGGLPGGSSKK157 pKa = 10.96SALKK161 pKa = 10.67GYY163 pKa = 10.67LSVAKK168 pKa = 9.45TNGVAPTVVAHH179 pKa = 7.22DD180 pKa = 4.04DD181 pKa = 3.65RR182 pKa = 11.84YY183 pKa = 9.36FAQVGTDD190 pKa = 3.71PPTMAFLQVAAQGAWAGQVFDD211 pKa = 3.95VQVDD215 pKa = 3.45LTYY218 pKa = 10.83YY219 pKa = 10.84VEE221 pKa = 5.22FFDD224 pKa = 6.01LVTPSPSS231 pKa = 2.85

Molecular weight: 26.47 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

644

101

312

214.7

24.94

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.969 ± 1.888

1.708 ± 0.501

5.124 ± 0.579

6.056 ± 1.925

2.64 ± 0.991

5.901 ± 1.496

2.795 ± 0.729

7.143 ± 1.757

5.901 ± 0.945

6.056 ± 0.992

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.261 ± 0.505

6.211 ± 1.264

4.658 ± 0.691

4.814 ± 0.578

7.609 ± 1.675

5.901 ± 1.437

6.522 ± 1.038

5.124 ± 1.659

1.553 ± 0.173

6.056 ± 0.295
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski