Sphingobacterium sp. CZ-2

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Sphingobacteriia; Sphingobacteriales; Sphingobacteriaceae; Sphingobacterium; unclassified Sphingobacterium

Average proteome isoelectric point is 6.54

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3302 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4P7DU53|A0A4P7DU53_9SPHI EVE domain-containing protein OS=Sphingobacterium sp. CZ-2 OX=2557994 GN=E3D81_08930 PE=4 SV=1
MM1 pKa = 7.19YY2 pKa = 7.77WTLEE6 pKa = 4.21LASHH10 pKa = 7.01LEE12 pKa = 4.08DD13 pKa = 4.93APWPATKK20 pKa = 10.54DD21 pKa = 3.44EE22 pKa = 5.26LIDD25 pKa = 3.48YY26 pKa = 8.56AIRR29 pKa = 11.84SGAPVEE35 pKa = 4.53VIEE38 pKa = 4.56NLQALEE44 pKa = 4.69DD45 pKa = 4.28DD46 pKa = 4.31GEE48 pKa = 4.31PYY50 pKa = 11.05EE51 pKa = 4.65NIEE54 pKa = 4.81EE55 pKa = 4.08IWPDD59 pKa = 3.85YY60 pKa = 7.47PTKK63 pKa = 10.94DD64 pKa = 3.05DD65 pKa = 4.15FFFNEE70 pKa = 4.51DD71 pKa = 3.17EE72 pKa = 4.46YY73 pKa = 11.99

Molecular weight: 8.61 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4P7DSY3|A0A4P7DSY3_9SPHI Gfo/Idh/MocA family oxidoreductase OS=Sphingobacterium sp. CZ-2 OX=2557994 GN=E3D81_11605 PE=4 SV=1
MM1 pKa = 7.85PVPKK5 pKa = 9.98IQQRR9 pKa = 11.84PSSLPAQRR17 pKa = 11.84RR18 pKa = 11.84NPKK21 pKa = 8.82NQNIQKK27 pKa = 9.52VARR30 pKa = 11.84ANPVKK35 pKa = 10.38NAKK38 pKa = 7.97VTNAGAVVPTALAGAVLQPLPP59 pKa = 4.09

Molecular weight: 6.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3302

0

3302

1148466

24

1972

347.8

39.25

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.872 ± 0.041

0.684 ± 0.013

5.477 ± 0.025

6.471 ± 0.038

5.043 ± 0.03

6.672 ± 0.036

1.884 ± 0.022

7.457 ± 0.034

7.298 ± 0.044

9.628 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.465 ± 0.02

5.652 ± 0.041

3.607 ± 0.024

3.879 ± 0.023

3.999 ± 0.026

6.421 ± 0.029

5.074 ± 0.029

6.171 ± 0.035

1.136 ± 0.016

4.108 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski