Amino acid dipepetide frequency for Sphingobacterium sp. CZ-2

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.193AlaAla: 5.193 ± 0.088
0.599AlaCys: 0.599 ± 0.025
3.916AlaAsp: 3.916 ± 0.07
4.516AlaGlu: 4.516 ± 0.068
3.22AlaPhe: 3.22 ± 0.059
4.913AlaGly: 4.913 ± 0.081
1.173AlaHis: 1.173 ± 0.034
5.377AlaIle: 5.377 ± 0.078
4.941AlaLys: 4.941 ± 0.084
6.558AlaLeu: 6.558 ± 0.084
1.807AlaMet: 1.807 ± 0.039
3.634AlaAsn: 3.634 ± 0.061
1.945AlaPro: 1.945 ± 0.046
2.71AlaGln: 2.71 ± 0.045
2.367AlaArg: 2.367 ± 0.049
4.361AlaSer: 4.361 ± 0.072
3.293AlaThr: 3.293 ± 0.062
4.562AlaVal: 4.562 ± 0.076
0.785AlaTrp: 0.785 ± 0.026
2.852AlaTyr: 2.852 ± 0.052
0.0AlaXaa: 0.0 ± 0.0
Cys
0.425CysAla: 0.425 ± 0.022
0.134CysCys: 0.134 ± 0.011
0.337CysAsp: 0.337 ± 0.018
0.335CysGlu: 0.335 ± 0.019
0.361CysPhe: 0.361 ± 0.017
0.549CysGly: 0.549 ± 0.025
0.185CysHis: 0.185 ± 0.014
0.523CysIle: 0.523 ± 0.025
0.423CysLys: 0.423 ± 0.024
0.738CysLeu: 0.738 ± 0.032
0.177CysMet: 0.177 ± 0.013
0.33CysAsn: 0.33 ± 0.018
0.287CysPro: 0.287 ± 0.018
0.231CysGln: 0.231 ± 0.015
0.267CysArg: 0.267 ± 0.017
0.493CysSer: 0.493 ± 0.022
0.347CysThr: 0.347 ± 0.017
0.359CysVal: 0.359 ± 0.019
0.059CysTrp: 0.059 ± 0.006
0.281CysTyr: 0.281 ± 0.018
0.0CysXaa: 0.0 ± 0.0
Asp
3.8AspAla: 3.8 ± 0.063
0.324AspCys: 0.324 ± 0.019
2.422AspAsp: 2.422 ± 0.049
3.539AspGlu: 3.539 ± 0.067
3.305AspPhe: 3.305 ± 0.053
3.794AspGly: 3.794 ± 0.065
1.109AspHis: 1.109 ± 0.039
4.038AspIle: 4.038 ± 0.059
3.986AspLys: 3.986 ± 0.061
5.558AspLeu: 5.558 ± 0.063
1.366AspMet: 1.366 ± 0.036
2.718AspAsn: 2.718 ± 0.05
2.212AspPro: 2.212 ± 0.046
2.179AspGln: 2.179 ± 0.044
2.492AspArg: 2.492 ± 0.045
3.0AspSer: 3.0 ± 0.063
2.132AspThr: 2.132 ± 0.048
3.382AspVal: 3.382 ± 0.052
0.778AspTrp: 0.778 ± 0.025
2.635AspTyr: 2.635 ± 0.05
0.0AspXaa: 0.0 ± 0.0
Glu
4.358GluAla: 4.358 ± 0.072
0.309GluCys: 0.309 ± 0.019
3.404GluAsp: 3.404 ± 0.066
5.127GluGlu: 5.127 ± 0.099
2.849GluPhe: 2.849 ± 0.056
3.816GluGly: 3.816 ± 0.057
1.249GluHis: 1.249 ± 0.03
5.249GluIle: 5.249 ± 0.076
5.598GluLys: 5.598 ± 0.086
6.342GluLeu: 6.342 ± 0.088
1.657GluMet: 1.657 ± 0.039
3.928GluAsn: 3.928 ± 0.063
1.673GluPro: 1.673 ± 0.042
2.666GluGln: 2.666 ± 0.06
2.924GluArg: 2.924 ± 0.057
3.317GluSer: 3.317 ± 0.057
3.017GluThr: 3.017 ± 0.054
4.398GluVal: 4.398 ± 0.065
0.657GluTrp: 0.657 ± 0.024
2.179GluTyr: 2.179 ± 0.052
0.0GluXaa: 0.0 ± 0.0
Phe
3.227PheAla: 3.227 ± 0.047
0.385PheCys: 0.385 ± 0.018
3.07PheAsp: 3.07 ± 0.055
3.061PheGlu: 3.061 ± 0.054
2.599PhePhe: 2.599 ± 0.059
3.589PheGly: 3.589 ± 0.069
0.994PheHis: 0.994 ± 0.026
3.465PheIle: 3.465 ± 0.058
3.285PheLys: 3.285 ± 0.057
4.762PheLeu: 4.762 ± 0.083
1.228PheMet: 1.228 ± 0.028
2.905PheAsn: 2.905 ± 0.065
1.893PhePro: 1.893 ± 0.042
1.917PheGln: 1.917 ± 0.046
1.98PheArg: 1.98 ± 0.04
3.781PheSer: 3.781 ± 0.07
2.603PheThr: 2.603 ± 0.043
3.036PheVal: 3.036 ± 0.051
0.591PheTrp: 0.591 ± 0.026
2.056PheTyr: 2.056 ± 0.042
0.0PheXaa: 0.0 ± 0.0
Gly
4.198GlyAla: 4.198 ± 0.07
0.536GlyCys: 0.536 ± 0.026
3.312GlyAsp: 3.312 ± 0.052
3.625GlyGlu: 3.625 ± 0.057
3.681GlyPhe: 3.681 ± 0.059
4.478GlyGly: 4.478 ± 0.088
1.185GlyHis: 1.185 ± 0.034
5.412GlyIle: 5.412 ± 0.071
5.502GlyLys: 5.502 ± 0.068
6.428GlyLeu: 6.428 ± 0.091
1.869GlyMet: 1.869 ± 0.047
3.705GlyAsn: 3.705 ± 0.073
1.513GlyPro: 1.513 ± 0.039
2.239GlyGln: 2.239 ± 0.044
2.712GlyArg: 2.712 ± 0.054
4.321GlySer: 4.321 ± 0.07
3.848GlyThr: 3.848 ± 0.064
4.186GlyVal: 4.186 ± 0.063
0.818GlyTrp: 0.818 ± 0.027
3.152GlyTyr: 3.152 ± 0.058
0.0GlyXaa: 0.0 ± 0.0
His
1.256HisAla: 1.256 ± 0.035
0.183HisCys: 0.183 ± 0.014
0.945HisAsp: 0.945 ± 0.038
1.073HisGlu: 1.073 ± 0.033
1.101HisPhe: 1.101 ± 0.038
1.196HisGly: 1.196 ± 0.039
0.473HisHis: 0.473 ± 0.02
1.504HisIle: 1.504 ± 0.036
1.049HisLys: 1.049 ± 0.029
1.891HisLeu: 1.891 ± 0.039
0.408HisMet: 0.408 ± 0.021
0.904HisAsn: 0.904 ± 0.034
0.988HisPro: 0.988 ± 0.029
0.874HisGln: 0.874 ± 0.03
0.825HisArg: 0.825 ± 0.029
1.088HisSer: 1.088 ± 0.027
0.918HisThr: 0.918 ± 0.029
1.065HisVal: 1.065 ± 0.03
0.226HisTrp: 0.226 ± 0.013
0.87HisTyr: 0.87 ± 0.027
0.0HisXaa: 0.0 ± 0.0
Ile
5.607IleAla: 5.607 ± 0.075
0.66IleCys: 0.66 ± 0.023
4.361IleAsp: 4.361 ± 0.063
4.48IleGlu: 4.48 ± 0.072
3.385IlePhe: 3.385 ± 0.061
5.065IleGly: 5.065 ± 0.074
1.476IleHis: 1.476 ± 0.039
5.294IleIle: 5.294 ± 0.079
5.17IleLys: 5.17 ± 0.073
6.985IleLeu: 6.985 ± 0.099
1.526IleMet: 1.526 ± 0.041
4.274IleAsn: 4.274 ± 0.067
3.382IlePro: 3.382 ± 0.054
2.98IleGln: 2.98 ± 0.059
3.075IleArg: 3.075 ± 0.053
5.395IleSer: 5.395 ± 0.065
3.701IleThr: 3.701 ± 0.053
4.235IleVal: 4.235 ± 0.065
0.761IleTrp: 0.761 ± 0.026
2.76IleTyr: 2.76 ± 0.052
0.0IleXaa: 0.0 ± 0.0
Lys
5.039LysAla: 5.039 ± 0.079
0.242LysCys: 0.242 ± 0.019
4.582LysAsp: 4.582 ± 0.064
5.897LysGlu: 5.897 ± 0.089
2.936LysPhe: 2.936 ± 0.053
4.644LysGly: 4.644 ± 0.07
1.377LysHis: 1.377 ± 0.038
5.3LysIle: 5.3 ± 0.083
5.653LysLys: 5.653 ± 0.088
6.24LysLeu: 6.24 ± 0.068
2.001LysMet: 2.001 ± 0.039
4.371LysAsn: 4.371 ± 0.071
2.621LysPro: 2.621 ± 0.047
2.804LysGln: 2.804 ± 0.05
2.954LysArg: 2.954 ± 0.055
4.287LysSer: 4.287 ± 0.067
3.876LysThr: 3.876 ± 0.056
4.53LysVal: 4.53 ± 0.062
0.753LysTrp: 0.753 ± 0.026
2.879LysTyr: 2.879 ± 0.046
0.0LysXaa: 0.0 ± 0.0
Leu
6.873LeuAla: 6.873 ± 0.093
0.684LeuCys: 0.684 ± 0.023
5.438LeuAsp: 5.438 ± 0.073
6.026LeuGlu: 6.026 ± 0.089
4.895LeuPhe: 4.895 ± 0.074
6.38LeuGly: 6.38 ± 0.093
1.703LeuHis: 1.703 ± 0.041
6.839LeuIle: 6.839 ± 0.093
7.281LeuLys: 7.281 ± 0.088
9.359LeuLeu: 9.359 ± 0.123
2.506LeuMet: 2.506 ± 0.048
5.491LeuAsn: 5.491 ± 0.073
3.8LeuPro: 3.8 ± 0.06
3.681LeuGln: 3.681 ± 0.064
3.701LeuArg: 3.701 ± 0.054
6.921LeuSer: 6.921 ± 0.087
4.981LeuThr: 4.981 ± 0.065
5.493LeuVal: 5.493 ± 0.075
0.876LeuTrp: 0.876 ± 0.032
3.356LeuTyr: 3.356 ± 0.055
0.0LeuXaa: 0.0 ± 0.0
Met
1.92MetAla: 1.92 ± 0.051
0.13MetCys: 0.13 ± 0.01
1.504MetAsp: 1.504 ± 0.033
1.889MetGlu: 1.889 ± 0.04
0.925MetPhe: 0.925 ± 0.029
1.672MetGly: 1.672 ± 0.038
0.428MetHis: 0.428 ± 0.02
1.566MetIle: 1.566 ± 0.037
2.094MetLys: 2.094 ± 0.041
2.258MetLeu: 2.258 ± 0.046
0.714MetMet: 0.714 ± 0.026
1.457MetAsn: 1.457 ± 0.039
0.972MetPro: 0.972 ± 0.032
0.953MetGln: 0.953 ± 0.031
1.045MetArg: 1.045 ± 0.031
1.425MetSer: 1.425 ± 0.037
1.22MetThr: 1.22 ± 0.034
1.559MetVal: 1.559 ± 0.038
0.201MetTrp: 0.201 ± 0.015
0.716MetTyr: 0.716 ± 0.025
0.0MetXaa: 0.0 ± 0.0
Asn
3.521AsnAla: 3.521 ± 0.063
0.299AsnCys: 0.299 ± 0.016
2.67AsnAsp: 2.67 ± 0.046
3.115AsnGlu: 3.115 ± 0.055
2.874AsnPhe: 2.874 ± 0.055
3.757AsnGly: 3.757 ± 0.076
1.06AsnHis: 1.06 ± 0.032
4.243AsnIle: 4.243 ± 0.067
3.998AsnLys: 3.998 ± 0.068
5.586AsnLeu: 5.586 ± 0.083
1.346AsnMet: 1.346 ± 0.039
3.338AsnAsn: 3.338 ± 0.082
2.844AsnPro: 2.844 ± 0.052
2.45AsnGln: 2.45 ± 0.049
2.516AsnArg: 2.516 ± 0.049
3.511AsnSer: 3.511 ± 0.064
2.866AsnThr: 2.866 ± 0.062
3.143AsnVal: 3.143 ± 0.051
0.778AsnTrp: 0.778 ± 0.027
2.609AsnTyr: 2.609 ± 0.055
0.0AsnXaa: 0.0 ± 0.0
Pro
2.496ProAla: 2.496 ± 0.051
0.213ProCys: 0.213 ± 0.014
2.13ProAsp: 2.13 ± 0.047
2.832ProGlu: 2.832 ± 0.049
1.882ProPhe: 1.882 ± 0.041
2.278ProGly: 2.278 ± 0.053
0.676ProHis: 0.676 ± 0.024
2.827ProIle: 2.827 ± 0.051
2.547ProLys: 2.547 ± 0.043
3.216ProLeu: 3.216 ± 0.057
0.796ProMet: 0.796 ± 0.027
2.196ProAsn: 2.196 ± 0.046
0.739ProPro: 0.739 ± 0.028
1.27ProGln: 1.27 ± 0.036
1.133ProArg: 1.133 ± 0.034
2.371ProSer: 2.371 ± 0.049
2.038ProThr: 2.038 ± 0.048
2.504ProVal: 2.504 ± 0.052
0.392ProTrp: 0.392 ± 0.018
1.536ProTyr: 1.536 ± 0.039
0.0ProXaa: 0.0 ± 0.0
Gln
2.721GlnAla: 2.721 ± 0.049
0.16GlnCys: 0.16 ± 0.012
1.995GlnAsp: 1.995 ± 0.043
3.0GlnGlu: 3.0 ± 0.059
1.763GlnPhe: 1.763 ± 0.043
2.335GlnGly: 2.335 ± 0.043
0.84GlnHis: 0.84 ± 0.025
2.904GlnIle: 2.904 ± 0.05
2.806GlnLys: 2.806 ± 0.061
3.851GlnLeu: 3.851 ± 0.066
0.905GlnMet: 0.905 ± 0.028
1.976GlnAsn: 1.976 ± 0.043
1.189GlnPro: 1.189 ± 0.033
2.039GlnGln: 2.039 ± 0.053
1.573GlnArg: 1.573 ± 0.04
2.129GlnSer: 2.129 ± 0.043
2.033GlnThr: 2.033 ± 0.043
2.608GlnVal: 2.608 ± 0.049
0.423GlnTrp: 0.423 ± 0.018
1.537GlnTyr: 1.537 ± 0.038
0.0GlnXaa: 0.0 ± 0.0
Arg
2.462ArgAla: 2.462 ± 0.049
0.217ArgCys: 0.217 ± 0.014
2.015ArgAsp: 2.015 ± 0.038
2.566ArgGlu: 2.566 ± 0.049
2.223ArgPhe: 2.223 ± 0.042
2.237ArgGly: 2.237 ± 0.049
0.684ArgHis: 0.684 ± 0.026
3.301ArgIle: 3.301 ± 0.051
3.196ArgLys: 3.196 ± 0.062
3.793ArgLeu: 3.793 ± 0.068
1.162ArgMet: 1.162 ± 0.034
2.346ArgAsn: 2.346 ± 0.046
1.364ArgPro: 1.364 ± 0.037
1.397ArgGln: 1.397 ± 0.034
1.63ArgArg: 1.63 ± 0.04
2.438ArgSer: 2.438 ± 0.057
2.12ArgThr: 2.12 ± 0.04
2.475ArgVal: 2.475 ± 0.047
0.515ArgTrp: 0.515 ± 0.021
1.854ArgTyr: 1.854 ± 0.041
0.0ArgXaa: 0.0 ± 0.0
Ser
4.063SerAla: 4.063 ± 0.063
0.599SerCys: 0.599 ± 0.021
3.187SerAsp: 3.187 ± 0.052
3.603SerGlu: 3.603 ± 0.054
3.637SerPhe: 3.637 ± 0.063
4.486SerGly: 4.486 ± 0.073
1.102SerHis: 1.102 ± 0.032
5.132SerIle: 5.132 ± 0.068
4.454SerLys: 4.454 ± 0.061
6.546SerLeu: 6.546 ± 0.091
1.487SerMet: 1.487 ± 0.032
3.711SerAsn: 3.711 ± 0.067
2.27SerPro: 2.27 ± 0.041
2.125SerGln: 2.125 ± 0.042
2.537SerArg: 2.537 ± 0.05
4.48SerSer: 4.48 ± 0.083
3.431SerThr: 3.431 ± 0.064
3.783SerVal: 3.783 ± 0.057
0.748SerTrp: 0.748 ± 0.03
2.827SerTyr: 2.827 ± 0.05
0.0SerXaa: 0.0 ± 0.0
Thr
3.844ThrAla: 3.844 ± 0.062
0.307ThrCys: 0.307 ± 0.016
2.842ThrAsp: 2.842 ± 0.048
3.033ThrGlu: 3.033 ± 0.056
2.64ThrPhe: 2.64 ± 0.057
3.894ThrGly: 3.894 ± 0.06
0.906ThrHis: 0.906 ± 0.029
3.856ThrIle: 3.856 ± 0.062
3.158ThrLys: 3.158 ± 0.047
4.846ThrLeu: 4.846 ± 0.066
1.034ThrMet: 1.034 ± 0.029
2.647ThrAsn: 2.647 ± 0.053
2.059ThrPro: 2.059 ± 0.043
1.7ThrGln: 1.7 ± 0.038
1.668ThrArg: 1.668 ± 0.04
3.243ThrSer: 3.243 ± 0.055
2.702ThrThr: 2.702 ± 0.054
3.462ThrVal: 3.462 ± 0.059
0.657ThrTrp: 0.657 ± 0.029
2.24ThrTyr: 2.24 ± 0.041
0.0ThrXaa: 0.0 ± 0.0
Val
4.213ValAla: 4.213 ± 0.071
0.457ValCys: 0.457 ± 0.021
3.721ValAsp: 3.721 ± 0.061
3.987ValGlu: 3.987 ± 0.057
3.202ValPhe: 3.202 ± 0.055
4.057ValGly: 4.057 ± 0.081
1.068ValHis: 1.068 ± 0.03
4.378ValIle: 4.378 ± 0.064
4.308ValLys: 4.308 ± 0.068
6.2ValLeu: 6.2 ± 0.071
1.437ValMet: 1.437 ± 0.037
3.541ValAsn: 3.541 ± 0.058
2.356ValPro: 2.356 ± 0.049
2.251ValGln: 2.251 ± 0.038
2.352ValArg: 2.352 ± 0.043
4.22ValSer: 4.22 ± 0.065
2.898ValThr: 2.898 ± 0.051
4.079ValVal: 4.079 ± 0.074
0.657ValTrp: 0.657 ± 0.027
2.333ValTyr: 2.333 ± 0.047
0.0ValXaa: 0.0 ± 0.0
Trp
0.718TrpAla: 0.718 ± 0.027
0.096TrpCys: 0.096 ± 0.009
0.622TrpAsp: 0.622 ± 0.027
0.72TrpGlu: 0.72 ± 0.025
0.562TrpPhe: 0.562 ± 0.027
0.764TrpGly: 0.764 ± 0.033
0.223TrpHis: 0.223 ± 0.014
0.787TrpIle: 0.787 ± 0.028
0.923TrpLys: 0.923 ± 0.028
1.058TrpLeu: 1.058 ± 0.033
0.365TrpMet: 0.365 ± 0.02
0.721TrpAsn: 0.721 ± 0.025
0.27TrpPro: 0.27 ± 0.017
0.454TrpGln: 0.454 ± 0.022
0.516TrpArg: 0.516 ± 0.022
0.683TrpSer: 0.683 ± 0.025
0.63TrpThr: 0.63 ± 0.027
0.652TrpVal: 0.652 ± 0.025
0.142TrpTrp: 0.142 ± 0.011
0.454TrpTyr: 0.454 ± 0.018
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.789TyrAla: 2.789 ± 0.048
0.31TyrCys: 0.31 ± 0.02
2.299TyrAsp: 2.299 ± 0.042
2.293TyrGlu: 2.293 ± 0.044
2.386TyrPhe: 2.386 ± 0.045
2.819TyrGly: 2.819 ± 0.057
0.926TyrHis: 0.926 ± 0.033
2.576TyrIle: 2.576 ± 0.049
2.612TyrLys: 2.612 ± 0.052
4.066TyrLeu: 4.066 ± 0.068
0.856TyrMet: 0.856 ± 0.027
2.33TyrAsn: 2.33 ± 0.055
1.694TyrPro: 1.694 ± 0.038
1.867TyrGln: 1.867 ± 0.043
1.728TyrArg: 1.728 ± 0.043
2.745TyrSer: 2.745 ± 0.049
2.087TyrThr: 2.087 ± 0.05
2.2TyrVal: 2.2 ± 0.039
0.541TyrTrp: 0.541 ± 0.022
1.96TyrTyr: 1.96 ± 0.043
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3302 proteins (1148466 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski