Metabacillus litoralis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Metabacillus

Average proteome isoelectric point is 6.24

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4919 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A179T3M1|A0A179T3M1_9BACI Beta-galactosidase OS=Metabacillus litoralis OX=152268 GN=A6K24_17855 PE=3 SV=1
MM1 pKa = 7.58LSSQQVEE8 pKa = 4.47TFRR11 pKa = 11.84SQLLKK16 pKa = 10.45MKK18 pKa = 10.67DD19 pKa = 3.32EE20 pKa = 4.2IEE22 pKa = 4.3QRR24 pKa = 11.84FEE26 pKa = 4.16MNGHH30 pKa = 5.87YY31 pKa = 10.39GLEE34 pKa = 4.09EE35 pKa = 4.0GHH37 pKa = 6.23AHH39 pKa = 6.1EE40 pKa = 5.26SVGEE44 pKa = 3.8LSSYY48 pKa = 11.08DD49 pKa = 3.38NHH51 pKa = 6.93PADD54 pKa = 4.36EE55 pKa = 4.21ATEE58 pKa = 4.28LYY60 pKa = 10.44EE61 pKa = 4.18RR62 pKa = 11.84EE63 pKa = 3.81KK64 pKa = 11.05DD65 pKa = 3.16IALNEE70 pKa = 4.15HH71 pKa = 5.71TEE73 pKa = 4.09EE74 pKa = 4.25EE75 pKa = 4.26LTDD78 pKa = 3.42IDD80 pKa = 4.4RR81 pKa = 11.84ALQAVDD87 pKa = 2.62NGTYY91 pKa = 10.25GKK93 pKa = 10.76CEE95 pKa = 3.89VCGMEE100 pKa = 4.69IPLDD104 pKa = 3.87RR105 pKa = 11.84LDD107 pKa = 5.53AIPTATTCKK116 pKa = 9.53EE117 pKa = 3.94HH118 pKa = 8.0APEE121 pKa = 4.03QVVSHH126 pKa = 6.01NRR128 pKa = 11.84PIEE131 pKa = 4.24EE132 pKa = 4.32GVLMPPFGKK141 pKa = 10.32FNYY144 pKa = 9.75DD145 pKa = 3.49DD146 pKa = 3.54QDD148 pKa = 3.66EE149 pKa = 4.45NVAFDD154 pKa = 5.5AEE156 pKa = 4.08DD157 pKa = 3.87AYY159 pKa = 11.3QIVNSFGSSEE169 pKa = 4.3TPSDD173 pKa = 3.15LTYY176 pKa = 10.86PKK178 pKa = 10.64DD179 pKa = 3.7HH180 pKa = 6.77YY181 pKa = 11.35NDD183 pKa = 3.34MYY185 pKa = 10.59MEE187 pKa = 4.36SEE189 pKa = 4.18EE190 pKa = 4.02PDD192 pKa = 4.01GYY194 pKa = 11.62VEE196 pKa = 5.13DD197 pKa = 4.32YY198 pKa = 10.96EE199 pKa = 5.14NFVGTDD205 pKa = 3.22IEE207 pKa = 4.53GKK209 pKa = 10.17EE210 pKa = 3.64ITVYY214 pKa = 10.52PSNQHH219 pKa = 4.79EE220 pKa = 4.49QYY222 pKa = 11.43EE223 pKa = 4.28DD224 pKa = 3.44MLDD227 pKa = 3.6EE228 pKa = 4.99EE229 pKa = 5.06GLMTIFGDD237 pKa = 3.66LPGYY241 pKa = 10.36EE242 pKa = 4.05KK243 pKa = 10.87DD244 pKa = 3.46PYY246 pKa = 10.4TEE248 pKa = 4.23EE249 pKa = 4.16EE250 pKa = 4.38VV251 pKa = 3.4

Molecular weight: 28.73 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A179SSJ2|A0A179SSJ2_9BACI Peptidase M20 OS=Metabacillus litoralis OX=152268 GN=A6K24_07755 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.42RR3 pKa = 11.84TYY5 pKa = 10.34QPNKK9 pKa = 8.21RR10 pKa = 11.84KK11 pKa = 9.62RR12 pKa = 11.84SKK14 pKa = 9.59VHH16 pKa = 5.83GFRR19 pKa = 11.84SRR21 pKa = 11.84MSTANGRR28 pKa = 11.84KK29 pKa = 8.48VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 8.94VLSAA44 pKa = 4.11

Molecular weight: 5.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4919

0

4919

1507023

26

2126

306.4

34.48

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.404 ± 0.036

0.73 ± 0.009

5.111 ± 0.027

7.445 ± 0.035

4.672 ± 0.03

6.713 ± 0.034

2.081 ± 0.016

8.308 ± 0.039

6.996 ± 0.032

9.803 ± 0.037

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.651 ± 0.015

4.848 ± 0.027

3.524 ± 0.021

3.698 ± 0.023

3.653 ± 0.021

6.276 ± 0.024

5.502 ± 0.023

6.853 ± 0.027

1.101 ± 0.014

3.632 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski