Amino acid dipepetide frequency for Metabacillus litoralis

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	4.78AlaAla: 4.78 ± 0.069	0.539AlaCys: 0.539 ± 0.019	3.035AlaAsp: 3.035 ± 0.049	4.169AlaGlu: 4.169 ± 0.066	3.1AlaPhe: 3.1 ± 0.055	4.695AlaGly: 4.695 ± 0.07	1.174AlaHis: 1.174 ± 0.032	5.782AlaIle: 5.782 ± 0.071	4.561AlaLys: 4.561 ± 0.06	6.575AlaLeu: 6.575 ± 0.081	1.824AlaMet: 1.824 ± 0.034	2.993AlaAsn: 2.993 ± 0.044	1.883AlaPro: 1.883 ± 0.04	1.944AlaGln: 1.944 ± 0.033	2.186AlaArg: 2.186 ± 0.033	3.926AlaSer: 3.926 ± 0.059	3.37AlaThr: 3.37 ± 0.054	4.741AlaVal: 4.741 ± 0.058	0.612AlaTrp: 0.612 ± 0.019	2.146AlaTyr: 2.146 ± 0.036	0.0AlaXaa: 0.0 ± 0.0
Cys	0.397CysAla: 0.397 ± 0.017	0.11CysCys: 0.11 ± 0.009	0.388CysAsp: 0.388 ± 0.015	0.497CysGlu: 0.497 ± 0.017	0.334CysPhe: 0.334 ± 0.016	0.625CysGly: 0.625 ± 0.021	0.21CysHis: 0.21 ± 0.012	0.563CysIle: 0.563 ± 0.021	0.405CysLys: 0.405 ± 0.017	0.696CysLeu: 0.696 ± 0.021	0.198CysMet: 0.198 ± 0.011	0.319CysAsn: 0.319 ± 0.013	0.342CysPro: 0.342 ± 0.015	0.226CysGln: 0.226 ± 0.012	0.274CysArg: 0.274 ± 0.015	0.563CysSer: 0.563 ± 0.019	0.388CysThr: 0.388 ± 0.014	0.437CysVal: 0.437 ± 0.018	0.08CysTrp: 0.08 ± 0.008	0.244CysTyr: 0.244 ± 0.014	0.0CysXaa: 0.0 ± 0.0
Asp	3.002AspAla: 3.002 ± 0.052	0.373AspCys: 0.373 ± 0.017	2.485AspAsp: 2.485 ± 0.046	4.452AspGlu: 4.452 ± 0.062	2.409AspPhe: 2.409 ± 0.042	3.387AspGly: 3.387 ± 0.063	1.267AspHis: 1.267 ± 0.031	4.245AspIle: 4.245 ± 0.058	3.204AspLys: 3.204 ± 0.053	5.106AspLeu: 5.106 ± 0.063	1.27AspMet: 1.27 ± 0.03	1.964AspAsn: 1.964 ± 0.038	2.005AspPro: 2.005 ± 0.039	2.135AspGln: 2.135 ± 0.041	2.096AspArg: 2.096 ± 0.038	2.802AspSer: 2.802 ± 0.046	2.426AspThr: 2.426 ± 0.039	3.611AspVal: 3.611 ± 0.049	0.673AspTrp: 0.673 ± 0.022	2.192AspTyr: 2.192 ± 0.041	0.0AspXaa: 0.0 ± 0.0
Glu	4.904GluAla: 4.904 ± 0.064	0.417GluCys: 0.417 ± 0.015	3.819GluAsp: 3.819 ± 0.049	6.762GluGlu: 6.762 ± 0.079	2.79GluPhe: 2.79 ± 0.044	4.29GluGly: 4.29 ± 0.072	1.565GluHis: 1.565 ± 0.035	5.947GluIle: 5.947 ± 0.063	6.486GluLys: 6.486 ± 0.073	7.178GluLeu: 7.178 ± 0.077	2.126GluMet: 2.126 ± 0.038	4.097GluAsn: 4.097 ± 0.056	1.851GluPro: 1.851 ± 0.034	3.307GluGln: 3.307 ± 0.054	3.11GluArg: 3.11 ± 0.05	3.667GluSer: 3.667 ± 0.052	3.829GluThr: 3.829 ± 0.056	5.028GluVal: 5.028 ± 0.063	0.848GluTrp: 0.848 ± 0.023	2.423GluTyr: 2.423 ± 0.044	0.0GluXaa: 0.0 ± 0.0
Phe	2.845PheAla: 2.845 ± 0.048	0.384PheCys: 0.384 ± 0.016	2.408PheAsp: 2.408 ± 0.043	2.927PheGlu: 2.927 ± 0.047	2.429PhePhe: 2.429 ± 0.053	3.299PheGly: 3.299 ± 0.055	1.037PheHis: 1.037 ± 0.03	4.237PheIle: 4.237 ± 0.072	2.668PheLys: 2.668 ± 0.043	4.675PheLeu: 4.675 ± 0.071	1.167PheMet: 1.167 ± 0.032	2.139PheAsn: 2.139 ± 0.044	1.656PhePro: 1.656 ± 0.035	1.642PheGln: 1.642 ± 0.032	1.51PheArg: 1.51 ± 0.033	3.429PheSer: 3.429 ± 0.048	2.754PheThr: 2.754 ± 0.047	3.186PheVal: 3.186 ± 0.052	0.536PheTrp: 0.536 ± 0.016	1.788PheTyr: 1.788 ± 0.038	0.0PheXaa: 0.0 ± 0.0
Gly	4.435GlyAla: 4.435 ± 0.07	0.635GlyCys: 0.635 ± 0.024	3.167GlyAsp: 3.167 ± 0.055	4.486GlyGlu: 4.486 ± 0.06	3.426GlyPhe: 3.426 ± 0.053	4.577GlyGly: 4.577 ± 0.066	1.287GlyHis: 1.287 ± 0.033	6.143GlyIle: 6.143 ± 0.088	4.824GlyLys: 4.824 ± 0.059	6.52GlyLeu: 6.52 ± 0.078	2.036GlyMet: 2.036 ± 0.038	2.993GlyAsn: 2.993 ± 0.053	1.717GlyPro: 1.717 ± 0.038	2.077GlyGln: 2.077 ± 0.042	2.36GlyArg: 2.36 ± 0.044	4.009GlySer: 4.009 ± 0.052	3.823GlyThr: 3.823 ± 0.055	4.935GlyVal: 4.935 ± 0.056	0.851GlyTrp: 0.851 ± 0.024	2.825GlyTyr: 2.825 ± 0.047	0.0GlyXaa: 0.0 ± 0.0
His	1.263HisAla: 1.263 ± 0.041	0.185HisCys: 0.185 ± 0.011	1.056HisAsp: 1.056 ± 0.029	1.396HisGlu: 1.396 ± 0.037	1.138HisPhe: 1.138 ± 0.027	1.324HisGly: 1.324 ± 0.035	0.638HisHis: 0.638 ± 0.023	1.62HisIle: 1.62 ± 0.031	1.121HisLys: 1.121 ± 0.029	2.123HisLeu: 2.123 ± 0.036	0.473HisMet: 0.473 ± 0.018	0.851HisAsn: 0.851 ± 0.024	1.087HisPro: 1.087 ± 0.029	0.83HisGln: 0.83 ± 0.023	0.805HisArg: 0.805 ± 0.025	1.291HisSer: 1.291 ± 0.031	1.066HisThr: 1.066 ± 0.027	1.352HisVal: 1.352 ± 0.032	0.252HisTrp: 0.252 ± 0.014	0.938HisTyr: 0.938 ± 0.027	0.0HisXaa: 0.0 ± 0.0
Ile	5.811IleAla: 5.811 ± 0.072	0.707IleCys: 0.707 ± 0.023	4.699IleAsp: 4.699 ± 0.059	6.289IleGlu: 6.289 ± 0.075	3.605IlePhe: 3.605 ± 0.061	6.233IleGly: 6.233 ± 0.078	1.721IleHis: 1.721 ± 0.037	6.669IleIle: 6.669 ± 0.104	5.107IleLys: 5.107 ± 0.068	7.469IleLeu: 7.469 ± 0.098	1.92IleMet: 1.92 ± 0.04	3.912IleAsn: 3.912 ± 0.054	3.506IlePro: 3.506 ± 0.05	2.971IleGln: 2.971 ± 0.043	2.959IleArg: 2.959 ± 0.051	5.613IleSer: 5.613 ± 0.069	4.658IleThr: 4.658 ± 0.056	5.762IleVal: 5.762 ± 0.073	0.764IleTrp: 0.764 ± 0.025	2.706IleTyr: 2.706 ± 0.047	0.0IleXaa: 0.0 ± 0.0
Lys	4.361LysAla: 4.361 ± 0.057	0.338LysCys: 0.338 ± 0.018	3.991LysAsp: 3.991 ± 0.059	6.719LysGlu: 6.719 ± 0.067	2.004LysPhe: 2.004 ± 0.036	4.425LysGly: 4.425 ± 0.061	1.417LysHis: 1.417 ± 0.029	5.082LysIle: 5.082 ± 0.069	5.699LysLys: 5.699 ± 0.075	6.162LysLeu: 6.162 ± 0.064	2.135LysMet: 2.135 ± 0.039	3.637LysAsn: 3.637 ± 0.049	2.214LysPro: 2.214 ± 0.038	3.371LysGln: 3.371 ± 0.046	3.086LysArg: 3.086 ± 0.05	3.655LysSer: 3.655 ± 0.051	3.733LysThr: 3.733 ± 0.046	4.772LysVal: 4.772 ± 0.054	0.866LysTrp: 0.866 ± 0.025	2.299LysTyr: 2.299 ± 0.037	0.0LysXaa: 0.0 ± 0.0
Leu	6.765LeuAla: 6.765 ± 0.065	0.705LeuCys: 0.705 ± 0.022	4.783LeuAsp: 4.783 ± 0.065	6.463LeuGlu: 6.463 ± 0.069	4.983LeuPhe: 4.983 ± 0.084	6.189LeuGly: 6.189 ± 0.076	1.904LeuHis: 1.904 ± 0.042	7.765LeuIle: 7.765 ± 0.106	6.812LeuLys: 6.812 ± 0.071	10.365LeuLeu: 10.365 ± 0.11	2.375LeuMet: 2.375 ± 0.041	4.642LeuAsn: 4.642 ± 0.056	3.91LeuPro: 3.91 ± 0.051	3.636LeuGln: 3.636 ± 0.054	3.224LeuArg: 3.224 ± 0.057	7.147LeuSer: 7.147 ± 0.085	5.874LeuThr: 5.874 ± 0.068	6.301LeuVal: 6.301 ± 0.063	0.912LeuTrp: 0.912 ± 0.027	3.278LeuTyr: 3.278 ± 0.051	0.0LeuXaa: 0.0 ± 0.0
Met	1.746MetAla: 1.746 ± 0.037	0.154MetCys: 0.154 ± 0.009	1.304MetAsp: 1.304 ± 0.029	1.764MetGlu: 1.764 ± 0.031	1.149MetPhe: 1.149 ± 0.03	1.642MetGly: 1.642 ± 0.042	0.409MetHis: 0.409 ± 0.015	2.345MetIle: 2.345 ± 0.042	2.478MetLys: 2.478 ± 0.039	2.473MetLeu: 2.473 ± 0.047	0.839MetMet: 0.839 ± 0.02	1.606MetAsn: 1.606 ± 0.035	0.928MetPro: 0.928 ± 0.027	0.818MetGln: 0.818 ± 0.02	0.889MetArg: 0.889 ± 0.028	1.647MetSer: 1.647 ± 0.036	1.583MetThr: 1.583 ± 0.03	1.777MetVal: 1.777 ± 0.03	0.2MetTrp: 0.2 ± 0.011	0.762MetTyr: 0.762 ± 0.023	0.0MetXaa: 0.0 ± 0.0
Asn	2.646AsnAla: 2.646 ± 0.043	0.336AsnCys: 0.336 ± 0.017	2.547AsnAsp: 2.547 ± 0.046	4.034AsnGlu: 4.034 ± 0.061	1.756AsnPhe: 1.756 ± 0.037	3.673AsnGly: 3.673 ± 0.061	1.158AsnHis: 1.158 ± 0.029	3.776AsnIle: 3.776 ± 0.051	3.399AsnLys: 3.399 ± 0.051	4.184AsnLeu: 4.184 ± 0.059	1.234AsnMet: 1.234 ± 0.029	2.399AsnAsn: 2.399 ± 0.043	2.23AsnPro: 2.23 ± 0.044	2.172AsnGln: 2.172 ± 0.045	2.02AsnArg: 2.02 ± 0.039	2.806AsnSer: 2.806 ± 0.042	2.384AsnThr: 2.384 ± 0.045	3.33AsnVal: 3.33 ± 0.049	0.634AsnTrp: 0.634 ± 0.02	1.762AsnTyr: 1.762 ± 0.034	0.0AsnXaa: 0.0 ± 0.0
Pro	2.051ProAla: 2.051 ± 0.038	0.227ProCys: 0.227 ± 0.011	1.896ProAsp: 1.896 ± 0.037	2.847ProGlu: 2.847 ± 0.05	2.034ProPhe: 2.034 ± 0.035	2.159ProGly: 2.159 ± 0.041	0.756ProHis: 0.756 ± 0.024	3.069ProIle: 3.069 ± 0.047	2.127ProLys: 2.127 ± 0.035	3.429ProLeu: 3.429 ± 0.049	0.827ProMet: 0.827 ± 0.026	1.884ProAsn: 1.884 ± 0.037	0.946ProPro: 0.946 ± 0.026	1.08ProGln: 1.08 ± 0.028	0.999ProArg: 0.999 ± 0.03	2.255ProSer: 2.255 ± 0.038	2.121ProThr: 2.121 ± 0.042	2.637ProVal: 2.637 ± 0.043	0.455ProTrp: 0.455 ± 0.02	1.438ProTyr: 1.438 ± 0.032	0.0ProXaa: 0.0 ± 0.0
Gln	2.522GlnAla: 2.522 ± 0.044	0.208GlnCys: 0.208 ± 0.013	1.705GlnAsp: 1.705 ± 0.036	2.742GlnGlu: 2.742 ± 0.039	1.753GlnPhe: 1.753 ± 0.028	2.145GlnGly: 2.145 ± 0.039	0.774GlnHis: 0.774 ± 0.023	2.823GlnIle: 2.823 ± 0.047	2.711GlnLys: 2.711 ± 0.046	4.059GlnLeu: 4.059 ± 0.052	1.035GlnMet: 1.035 ± 0.028	1.759GlnAsn: 1.759 ± 0.034	1.175GlnPro: 1.175 ± 0.026	1.816GlnGln: 1.816 ± 0.041	1.341GlnArg: 1.341 ± 0.031	2.235GlnSer: 2.235 ± 0.04	1.983GlnThr: 1.983 ± 0.037	2.37GlnVal: 2.37 ± 0.042	0.425GlnTrp: 0.425 ± 0.017	1.402GlnTyr: 1.402 ± 0.031	0.0GlnXaa: 0.0 ± 0.0
Arg	2.129ArgAla: 2.129 ± 0.046	0.231ArgCys: 0.231 ± 0.012	1.92ArgAsp: 1.92 ± 0.032	2.892ArgGlu: 2.892 ± 0.045	1.807ArgPhe: 1.807 ± 0.035	2.145ArgGly: 2.145 ± 0.039	0.733ArgHis: 0.733 ± 0.021	2.918ArgIle: 2.918 ± 0.05	2.902ArgLys: 2.902 ± 0.046	3.612ArgLeu: 3.612 ± 0.058	1.1ArgMet: 1.1 ± 0.027	1.901ArgAsn: 1.901 ± 0.037	1.178ArgPro: 1.178 ± 0.026	1.3ArgGln: 1.3 ± 0.028	1.531ArgArg: 1.531 ± 0.033	2.084ArgSer: 2.084 ± 0.036	1.926ArgThr: 1.926 ± 0.041	2.393ArgVal: 2.393 ± 0.042	0.37ArgTrp: 0.37 ± 0.015	1.461ArgTyr: 1.461 ± 0.033	0.0ArgXaa: 0.0 ± 0.0
Ser	3.607SerAla: 3.607 ± 0.052	0.415SerCys: 0.415 ± 0.017	2.916SerAsp: 2.916 ± 0.044	4.232SerGlu: 4.232 ± 0.058	3.522SerPhe: 3.522 ± 0.056	4.45SerGly: 4.45 ± 0.065	1.299SerHis: 1.299 ± 0.043	5.687SerIle: 5.687 ± 0.068	4.135SerLys: 4.135 ± 0.057	6.512SerLeu: 6.512 ± 0.079	1.655SerMet: 1.655 ± 0.038	2.944SerAsn: 2.944 ± 0.052	2.157SerPro: 2.157 ± 0.044	2.076SerGln: 2.076 ± 0.041	2.139SerArg: 2.139 ± 0.038	4.354SerSer: 4.354 ± 0.06	3.431SerThr: 3.431 ± 0.047	4.141SerVal: 4.141 ± 0.058	0.699SerTrp: 0.699 ± 0.021	2.383SerTyr: 2.383 ± 0.04	0.0SerXaa: 0.0 ± 0.0
Thr	3.583ThrAla: 3.583 ± 0.056	0.366ThrCys: 0.366 ± 0.016	2.743ThrAsp: 2.743 ± 0.049	3.552ThrGlu: 3.552 ± 0.049	2.831ThrPhe: 2.831 ± 0.047	3.979ThrGly: 3.979 ± 0.054	1.001ThrHis: 1.001 ± 0.029	5.032ThrIle: 5.032 ± 0.064	3.575ThrLys: 3.575 ± 0.048	5.448ThrLeu: 5.448 ± 0.06	1.295ThrMet: 1.295 ± 0.029	2.769ThrAsn: 2.769 ± 0.045	2.266ThrPro: 2.266 ± 0.043	1.437ThrGln: 1.437 ± 0.032	1.74ThrArg: 1.74 ± 0.036	3.611ThrSer: 3.611 ± 0.052	3.103ThrThr: 3.103 ± 0.05	4.034ThrVal: 4.034 ± 0.052	0.575ThrTrp: 0.575 ± 0.022	2.084ThrTyr: 2.084 ± 0.037	0.0ThrXaa: 0.0 ± 0.0
Val	4.588ValAla: 4.588 ± 0.063	0.565ValCys: 0.565 ± 0.023	3.668ValAsp: 3.668 ± 0.059	4.871ValGlu: 4.871 ± 0.06	3.137ValPhe: 3.137 ± 0.049	4.614ValGly: 4.614 ± 0.061	1.324ValHis: 1.324 ± 0.028	5.808ValIle: 5.808 ± 0.073	4.699ValLys: 4.699 ± 0.058	6.555ValLeu: 6.555 ± 0.072	1.757ValMet: 1.757 ± 0.034	3.389ValAsn: 3.389 ± 0.051	2.476ValPro: 2.476 ± 0.044	2.198ValGln: 2.198 ± 0.045	2.362ValArg: 2.362 ± 0.04	4.63ValSer: 4.63 ± 0.061	4.141ValThr: 4.141 ± 0.052	4.807ValVal: 4.807 ± 0.061	0.658ValTrp: 0.658 ± 0.021	2.281ValTyr: 2.281 ± 0.038	0.0ValXaa: 0.0 ± 0.0
Trp	0.635TrpAla: 0.635 ± 0.02	0.086TrpCys: 0.086 ± 0.008	0.594TrpAsp: 0.594 ± 0.022	0.731TrpGlu: 0.731 ± 0.023	0.534TrpPhe: 0.534 ± 0.022	0.745TrpGly: 0.745 ± 0.026	0.227TrpHis: 0.227 ± 0.012	0.869TrpIle: 0.869 ± 0.026	0.784TrpLys: 0.784 ± 0.027	1.197TrpLeu: 1.197 ± 0.032	0.372TrpMet: 0.372 ± 0.014	0.635TrpAsn: 0.635 ± 0.023	0.27TrpPro: 0.27 ± 0.017	0.396TrpGln: 0.396 ± 0.018	0.425TrpArg: 0.425 ± 0.015	0.721TrpSer: 0.721 ± 0.022	0.553TrpThr: 0.553 ± 0.022	0.687TrpVal: 0.687 ± 0.022	0.163TrpTrp: 0.163 ± 0.011	0.386TrpTyr: 0.386 ± 0.013	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.967TyrAla: 1.967 ± 0.041	0.315TyrCys: 0.315 ± 0.014	1.98TyrAsp: 1.98 ± 0.042	2.622TyrGlu: 2.622 ± 0.045	1.975TyrPhe: 1.975 ± 0.041	2.527TyrGly: 2.527 ± 0.041	0.91TyrHis: 0.91 ± 0.028	2.703TyrIle: 2.703 ± 0.049	2.267TyrLys: 2.267 ± 0.04	3.695TyrLeu: 3.695 ± 0.058	0.875TyrMet: 0.875 ± 0.022	1.646TyrAsn: 1.646 ± 0.034	1.441TyrPro: 1.441 ± 0.03	1.553TyrGln: 1.553 ± 0.032	1.474TyrArg: 1.474 ± 0.034	2.313TyrSer: 2.313 ± 0.041	1.875TyrThr: 1.875 ± 0.038	2.226TyrVal: 2.226 ± 0.04	0.436TyrTrp: 0.436 ± 0.016	1.524TyrTyr: 1.524 ± 0.036	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4919 proteins (1507023 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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